Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= brP-0327
(683 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At2g05630.1 68415.m00599 autophagy 8d (APG8d) identical to autop... 140 6e-34
At4g16520.2 68417.m02501 autophagy 8f (APG8f) identical to autop... 135 2e-32
At4g16520.1 68417.m02500 autophagy 8f (APG8f) identical to autop... 135 2e-32
At2g45170.2 68415.m05624 autophagy 8e (APG8e) identical to autop... 133 9e-32
At2g45170.1 68415.m05623 autophagy 8e (APG8e) identical to autop... 133 9e-32
At1g62040.1 68414.m06997 autophagy 8c (APG8c) identical to autop... 133 9e-32
At4g21980.1 68417.m03182 autophagy 8a (APG8a) identical to autop... 132 2e-31
At4g04620.2 68417.m00676 autophagy 8b (APG8b) identical to autop... 131 4e-31
At4g04620.1 68417.m00675 autophagy 8b (APG8b) identical to autop... 131 4e-31
At3g60640.1 68416.m06785 autophagy 8g (APG8g) identical to autop... 130 1e-30
At3g06420.1 68416.m00740 autophagy 8h (APG8h) identical to autop... 110 1e-24
At3g15580.1 68416.m01974 autophagy 8i (APG8i) identical to autop... 109 1e-24
At5g06680.1 68418.m00754 tubulin family protein similar to SP|Q9... 30 1.2
At4g19080.1 68417.m02813 hypothetical protein contains Pfam doma... 28 5.0
At1g75120.1 68414.m08724 expressed protein 28 5.0
At1g49050.1 68414.m05500 aspartyl protease family protein contai... 28 5.0
At5g54830.1 68418.m06829 DOMON domain-containing protein / dopam... 28 6.6
At4g28650.1 68417.m04095 leucine-rich repeat transmembrane prote... 28 6.6
>At2g05630.1 68415.m00599 autophagy 8d (APG8d) identical to
autophagy 8d [Arabidopsis thaliana] GI:19912157;
contains Pfam profile PF02991: Microtubule associated
protein 1A/1B, light chain 3
Length = 120
Score = 140 bits (340), Expect = 6e-34
Identities = 61/114 (53%), Positives = 86/114 (75%)
Frame = +3
Query: 66 SNTKKNIHLRRERLRAKKSAGKYPDRVPVIVEKAPKARLGDLDKKKYLVPSDLTVGQFYF 245
S+ K L + + A + KYPDR+PVIVE+A K+ + D+D+KKYLVP+DLTVGQF +
Sbjct: 4 SSFKHEHPLEKRQAEAARIREKYPDRIPVIVERAEKSDVPDIDRKKYLVPADLTVGQFVY 63
Query: 246 LIRKRIHLRPEDALFFFVNNVIPPTSATMGSLYQEHHDEDFFLYIAFSDENVYG 407
++RKRI L PE A+F FV N++PPT+A M ++Y+EH DED FLY+++S EN +G
Sbjct: 64 VVRKRIKLSPEKAIFIFVKNILPPTAAIMSAIYEEHKDEDGFLYMSYSGENTFG 117
>At4g16520.2 68417.m02501 autophagy 8f (APG8f) identical to
autophagy 8f [Arabidopsis thaliana] GI:19912161;
contains Pfam profile PF02991: Microtubule associated
protein 1A/1B, light chain 3
Length = 121
Score = 135 bits (327), Expect = 2e-32
Identities = 62/114 (54%), Positives = 82/114 (71%)
Frame = +3
Query: 66 SNTKKNIHLRRERLRAKKSAGKYPDRVPVIVEKAPKARLGDLDKKKYLVPSDLTVGQFYF 245
S+ K+ L + R A + KYPDR+PVIVEKA K+ + +DKKKYLVP+DLTVGQF +
Sbjct: 4 SSFKQEHDLEKRRAEAARIREKYPDRIPVIVEKAEKSDIPTIDKKKYLVPADLTVGQFVY 63
Query: 246 LIRKRIHLRPEDALFFFVNNVIPPTSATMGSLYQEHHDEDFFLYIAFSDENVYG 407
+IRKRI L E A+F FV+NV+PP A M S+Y+E D+D FLY+ +S EN +G
Sbjct: 64 VIRKRIKLSAEKAIFIFVDNVLPPAGALMSSVYEEKKDDDGFLYVTYSGENTFG 117
>At4g16520.1 68417.m02500 autophagy 8f (APG8f) identical to
autophagy 8f [Arabidopsis thaliana] GI:19912161;
contains Pfam profile PF02991: Microtubule associated
protein 1A/1B, light chain 3
Length = 121
Score = 135 bits (327), Expect = 2e-32
Identities = 62/114 (54%), Positives = 82/114 (71%)
Frame = +3
Query: 66 SNTKKNIHLRRERLRAKKSAGKYPDRVPVIVEKAPKARLGDLDKKKYLVPSDLTVGQFYF 245
S+ K+ L + R A + KYPDR+PVIVEKA K+ + +DKKKYLVP+DLTVGQF +
Sbjct: 4 SSFKQEHDLEKRRAEAARIREKYPDRIPVIVEKAEKSDIPTIDKKKYLVPADLTVGQFVY 63
Query: 246 LIRKRIHLRPEDALFFFVNNVIPPTSATMGSLYQEHHDEDFFLYIAFSDENVYG 407
+IRKRI L E A+F FV+NV+PP A M S+Y+E D+D FLY+ +S EN +G
Sbjct: 64 VIRKRIKLSAEKAIFIFVDNVLPPAGALMSSVYEEKKDDDGFLYVTYSGENTFG 117
>At2g45170.2 68415.m05624 autophagy 8e (APG8e) identical to
autophagy 8e [Arabidopsis thaliana] GI:19912159;
contains Pfam profile PF02991: Microtubule associated
protein 1A/1B, light chain 3
Length = 122
Score = 133 bits (322), Expect = 9e-32
Identities = 61/111 (54%), Positives = 80/111 (72%)
Frame = +3
Query: 75 KKNIHLRRERLRAKKSAGKYPDRVPVIVEKAPKARLGDLDKKKYLVPSDLTVGQFYFLIR 254
K + + + A + KYPDR+PVIVEKA K+ + ++DKKKYLVPSDLTVGQF ++IR
Sbjct: 8 KMDDDFEKRKAEAGRIREKYPDRIPVIVEKAEKSEVPNIDKKKYLVPSDLTVGQFVYVIR 67
Query: 255 KRIHLRPEDALFFFVNNVIPPTSATMGSLYQEHHDEDFFLYIAFSDENVYG 407
KRI L E A+F FV+NV+PPT M S+Y++ DED FLYI +S EN +G
Sbjct: 68 KRIKLSAEKAIFIFVDNVLPPTGELMSSVYEDKKDEDGFLYITYSGENTFG 118
>At2g45170.1 68415.m05623 autophagy 8e (APG8e) identical to
autophagy 8e [Arabidopsis thaliana] GI:19912159;
contains Pfam profile PF02991: Microtubule associated
protein 1A/1B, light chain 3
Length = 122
Score = 133 bits (322), Expect = 9e-32
Identities = 61/111 (54%), Positives = 80/111 (72%)
Frame = +3
Query: 75 KKNIHLRRERLRAKKSAGKYPDRVPVIVEKAPKARLGDLDKKKYLVPSDLTVGQFYFLIR 254
K + + + A + KYPDR+PVIVEKA K+ + ++DKKKYLVPSDLTVGQF ++IR
Sbjct: 8 KMDDDFEKRKAEAGRIREKYPDRIPVIVEKAEKSEVPNIDKKKYLVPSDLTVGQFVYVIR 67
Query: 255 KRIHLRPEDALFFFVNNVIPPTSATMGSLYQEHHDEDFFLYIAFSDENVYG 407
KRI L E A+F FV+NV+PPT M S+Y++ DED FLYI +S EN +G
Sbjct: 68 KRIKLSAEKAIFIFVDNVLPPTGELMSSVYEDKKDEDGFLYITYSGENTFG 118
>At1g62040.1 68414.m06997 autophagy 8c (APG8c) identical to
autophagy 8c [Arabidopsis thaliana] GI:19912155;
contains Pfam profile PF02991: Microtubule associated
protein 1A/1B, light chain 3
Length = 119
Score = 133 bits (322), Expect = 9e-32
Identities = 59/115 (51%), Positives = 84/115 (73%)
Frame = +3
Query: 63 NSNTKKNIHLRRERLRAKKSAGKYPDRVPVIVEKAPKARLGDLDKKKYLVPSDLTVGQFY 242
NS+ K L R ++ + + KYPDR+PVIVE+A ++ + ++DKKKYLVP+DLTVGQF
Sbjct: 3 NSSFKLEHPLERRQIESSRIREKYPDRIPVIVERAERSDVPNIDKKKYLVPADLTVGQFV 62
Query: 243 FLIRKRIHLRPEDALFFFVNNVIPPTSATMGSLYQEHHDEDFFLYIAFSDENVYG 407
+++RKRI L E A+F FV N +PPT+A M ++Y E+ DED FLY+ +S EN +G
Sbjct: 63 YVVRKRIKLSAEKAIFVFVKNTLPPTAAMMSAIYDENKDEDGFLYMTYSGENTFG 117
>At4g21980.1 68417.m03182 autophagy 8a (APG8a) identical to
autophagy 8a [Arabidopsis thaliana] GI:19912151;
contains Pfam profile PF02991: Microtubule associated
protein 1A/1B, light chain 3
Length = 122
Score = 132 bits (320), Expect = 2e-31
Identities = 57/94 (60%), Positives = 76/94 (80%)
Frame = +3
Query: 129 KYPDRVPVIVEKAPKARLGDLDKKKYLVPSDLTVGQFYFLIRKRIHLRPEDALFFFVNNV 308
KYPDR+PVIVEKA ++ + D+DKKKYLVP+DLTVGQF +++RKRI L E A+F FV N
Sbjct: 25 KYPDRIPVIVEKAGQSDVPDIDKKKYLVPADLTVGQFVYVVRKRIKLGAEKAIFVFVKNT 84
Query: 309 IPPTSATMGSLYQEHHDEDFFLYIAFSDENVYGN 410
+PPT+A M ++Y+EH DED FLY+ +S EN +G+
Sbjct: 85 LPPTAALMSAIYEEHKDEDGFLYMTYSGENTFGS 118
>At4g04620.2 68417.m00676 autophagy 8b (APG8b) identical to
autophagy 8b [Arabidopsis thaliana] GI:19912153;
contains Pfam profile PF02991: Microtubule associated
protein 1A/1B, light chain 3
Length = 122
Score = 131 bits (317), Expect = 4e-31
Identities = 57/96 (59%), Positives = 76/96 (79%)
Frame = +3
Query: 129 KYPDRVPVIVEKAPKARLGDLDKKKYLVPSDLTVGQFYFLIRKRIHLRPEDALFFFVNNV 308
KYP+RVPVIVEKA ++ + D+DKKKYLVP+DLT+GQF +++RKRI L E A+F FV N
Sbjct: 25 KYPERVPVIVEKAGQSDVPDIDKKKYLVPADLTIGQFVYVVRKRIKLGAEKAIFVFVKNT 84
Query: 309 IPPTSATMGSLYQEHHDEDFFLYIAFSDENVYGN*F 416
+PPT+A M ++Y+EH DED FLY+ +S EN +G F
Sbjct: 85 LPPTAALMSAIYEEHKDEDGFLYMTYSGENTFGGSF 120
>At4g04620.1 68417.m00675 autophagy 8b (APG8b) identical to
autophagy 8b [Arabidopsis thaliana] GI:19912153;
contains Pfam profile PF02991: Microtubule associated
protein 1A/1B, light chain 3
Length = 122
Score = 131 bits (317), Expect = 4e-31
Identities = 57/96 (59%), Positives = 76/96 (79%)
Frame = +3
Query: 129 KYPDRVPVIVEKAPKARLGDLDKKKYLVPSDLTVGQFYFLIRKRIHLRPEDALFFFVNNV 308
KYP+RVPVIVEKA ++ + D+DKKKYLVP+DLT+GQF +++RKRI L E A+F FV N
Sbjct: 25 KYPERVPVIVEKAGQSDVPDIDKKKYLVPADLTIGQFVYVVRKRIKLGAEKAIFVFVKNT 84
Query: 309 IPPTSATMGSLYQEHHDEDFFLYIAFSDENVYGN*F 416
+PPT+A M ++Y+EH DED FLY+ +S EN +G F
Sbjct: 85 LPPTAALMSAIYEEHKDEDGFLYMTYSGENTFGGSF 120
>At3g60640.1 68416.m06785 autophagy 8g (APG8g) identical to
autophagy 8g [Arabidopsis thaliana] GI:19912163;
contains Pfam profile PF02991: Microtubule associated
protein 1A/1B, light chain 3; supporting cDNA
gi|19912162|dbj|AB073181.1|
Length = 121
Score = 130 bits (313), Expect = 1e-30
Identities = 58/116 (50%), Positives = 84/116 (72%)
Frame = +3
Query: 63 NSNTKKNIHLRRERLRAKKSAGKYPDRVPVIVEKAPKARLGDLDKKKYLVPSDLTVGQFY 242
N + +++ + + A + KY DRVPVIVEK+ K+ + ++DKKKYLVP+DLTVGQF
Sbjct: 3 NVSFRQDHDFEKRKAEALRIREKYSDRVPVIVEKSEKSDIPNIDKKKYLVPADLTVGQFV 62
Query: 243 FLIRKRIHLRPEDALFFFVNNVIPPTSATMGSLYQEHHDEDFFLYIAFSDENVYGN 410
++IRKRI L E A+F FV+NV+PPT A M ++Y E+ +ED FLY+ +S EN +G+
Sbjct: 63 YVIRKRIQLSAEKAIFIFVDNVLPPTGAMMSTIYDENKEEDGFLYVTYSGENTFGS 118
>At3g06420.1 68416.m00740 autophagy 8h (APG8h) identical to
autophagy 8h [Arabidopsis thaliana] GI:19912165;
contains Pfam profile PF02991: Microtubule associated
protein 1A/1B, light chain 3; supporting cDNA
gi|19912164|dbj|AB073182.1|
Length = 119
Score = 110 bits (264), Expect = 1e-24
Identities = 51/104 (49%), Positives = 69/104 (66%), Gaps = 1/104 (0%)
Frame = +3
Query: 99 ERLRAKKSA-GKYPDRVPVIVEKAPKARLGDLDKKKYLVPSDLTVGQFYFLIRKRIHLRP 275
ERL+ + KYPDR+PVI+EK A L D++K KYLVP D+TVG F ++ KR+ L P
Sbjct: 16 ERLKESNNIIAKYPDRIPVIIEKYSNADLPDMEKNKYLVPRDMTVGHFIHMLSKRMQLDP 75
Query: 276 EDALFFFVNNVIPPTSATMGSLYQEHHDEDFFLYIAFSDENVYG 407
ALF FV+N +P T++ M SLY +ED FLY+ +S E +G
Sbjct: 76 SKALFVFVHNTLPQTASRMDSLYNTFKEEDGFLYMCYSTEKTFG 119
>At3g15580.1 68416.m01974 autophagy 8i (APG8i) identical to
autophagy 8i [Arabidopsis thaliana] GI:19912167;
contains Pfam profile PF02991: Microtubule associated
protein 1A/1B, light chain 3; supporting cDNA
gi|21636957|gb|AF492760.1|
Length = 115
Score = 109 bits (263), Expect = 1e-24
Identities = 49/104 (47%), Positives = 72/104 (69%), Gaps = 1/104 (0%)
Frame = +3
Query: 99 ERL-RAKKSAGKYPDRVPVIVEKAPKARLGDLDKKKYLVPSDLTVGQFYFLIRKRIHLRP 275
ERL +++ KYP R+PVI EK K L ++KKK+LVP D++VGQF +++ R+HL P
Sbjct: 12 ERLAESREIIAKYPTRIPVIAEKYCKTDLPAIEKKKFLVPRDMSVGQFIYILSARLHLSP 71
Query: 276 EDALFFFVNNVIPPTSATMGSLYQEHHDEDFFLYIAFSDENVYG 407
ALF FVNN +P T+A M S+Y+ + D+D F+Y+ +S E +G
Sbjct: 72 GKALFVFVNNTLPQTAALMDSVYESYKDDDGFVYMCYSSEKTFG 115
>At5g06680.1 68418.m00754 tubulin family protein similar to
SP|Q96CW5 Gamma-tubulin complex component 3 {Homo
sapiens}
Length = 838
Score = 30.3 bits (65), Expect = 1.2
Identities = 15/37 (40%), Positives = 19/37 (51%), Gaps = 1/37 (2%)
Frame = +2
Query: 401 LWKLI-PNYSLSNSFTLSDHMVKFNLSLRLKLCIILW 508
+WK + PN SNSF VK L L+ C +LW
Sbjct: 622 IWKTMKPNCITSNSFVKLQSSVKLQLLSALRRCQVLW 658
>At4g19080.1 68417.m02813 hypothetical protein contains Pfam domain,
PF04578: Protein of unknown function, DUF594
Length = 327
Score = 28.3 bits (60), Expect = 5.0
Identities = 18/63 (28%), Positives = 32/63 (50%)
Frame = +2
Query: 362 RFLFIHSIFRRKCLWKLIPNYSLSNSFTLSDHMVKFNLSLRLKLCIILWYFFYY*LAYNT 541
R L+ +F R CL + NY L F++SD + + + R +L LW F + + + +
Sbjct: 13 RTLYPFRLFIRFCLGIPLINYLLE-FFSISDQVNEIVFTSRTRLTKELWEFIFEEVKHRS 71
Query: 542 IFV 550
+ V
Sbjct: 72 LLV 74
>At1g75120.1 68414.m08724 expressed protein
Length = 402
Score = 28.3 bits (60), Expect = 5.0
Identities = 12/34 (35%), Positives = 18/34 (52%)
Frame = -1
Query: 179 PSLRRFLYNYRNAIWIFXCGFFRPQPFSSQMNVL 78
P++ R Y N IW+F GFF +P + +L
Sbjct: 266 PTMTRSRTVYTNRIWVFNSGFFYLRPTLPSIELL 299
>At1g49050.1 68414.m05500 aspartyl protease family protein contains
Pfam PF00026: Eukaryotic aspartyl protease; contains
similarity to nucellin GI:2290203 from [Hordeum vulgare]
Length = 583
Score = 28.3 bits (60), Expect = 5.0
Identities = 11/32 (34%), Positives = 20/32 (62%)
Frame = +3
Query: 192 DKKKYLVPSDLTVGQFYFLIRKRIHLRPEDAL 287
D KK+ P L +G + +I +++ ++PED L
Sbjct: 485 DVKKFFRPITLQIGSKWLIISRKLLIQPEDYL 516
>At5g54830.1 68418.m06829 DOMON domain-containing protein / dopamine
beta-monooxygenase N-terminal domain-containing protein
contains Pfam PF03351: DOMON domain
Length = 907
Score = 27.9 bits (59), Expect = 6.6
Identities = 17/39 (43%), Positives = 22/39 (56%), Gaps = 5/39 (12%)
Frame = +2
Query: 368 LFIHSIFRRKCLWKL-IPNYSLS----NSFTLSDHMVKF 469
L+ HSIF+ K WK+ + + LS NS L D VKF
Sbjct: 490 LYYHSIFQEKMGWKVQVVDGGLSDMYNNSVNLDDQQVKF 528
>At4g28650.1 68417.m04095 leucine-rich repeat transmembrane protein
kinase, putative receptor-like protein kinase 5,
Arabidopsis thaliana, PIR1:S27756
Length = 1013
Score = 27.9 bits (59), Expect = 6.6
Identities = 26/84 (30%), Positives = 34/84 (40%), Gaps = 2/84 (2%)
Frame = -3
Query: 318 WVE*HCSRRRKV-RLQAAGEYVSGSRSRT-GQLLNQTELNTSFCRGXXXXXXXXXXXXQE 145
W C+ V +L AG ++G S + QL + N S C G
Sbjct: 62 WTGVRCNSNGNVEKLDLAGMNLTGKISDSISQLSSLVSFNIS-CNGFESLLPKSIPPL-- 118
Query: 144 RDLDIXLRIFSPSAFLFSNECSSL 73
+ +DI FS S FLFSNE L
Sbjct: 119 KSIDISQNSFSGSLFLFSNESLGL 142
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 13,280,705
Number of Sequences: 28952
Number of extensions: 269954
Number of successful extensions: 673
Number of sequences better than 10.0: 18
Number of HSP's better than 10.0 without gapping: 654
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 671
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1447936096
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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