BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= brP-0319 (500 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At2g02580.1 68415.m00198 cytochrome P450 family protein 31 0.33 At3g61220.1 68416.m06851 short-chain dehydrogenase/reductase (SD... 31 0.58 At2g03550.1 68415.m00315 expressed protein similar to PrMC3 [Pin... 30 0.76 At1g19190.1 68414.m02389 expressed protein contains similarity t... 30 0.76 At4g37160.1 68417.m05261 multi-copper oxidase type I family prot... 29 1.3 At2g24930.1 68415.m02981 Ulp1 protease family protein contains P... 29 1.3 At2g24190.1 68415.m02890 short-chain dehydrogenase/reductase (SD... 29 1.3 At1g76590.1 68414.m08912 zinc-binding family protein similar to ... 29 2.3 At3g26300.1 68416.m03282 cytochrome P450 family protein contains... 27 5.4 At2g23630.1 68415.m02819 multi-copper oxidase type I family prot... 27 5.4 At2g17580.1 68415.m02034 polynucleotide adenylyltransferase fami... 27 5.4 At1g71800.1 68414.m08298 cleavage stimulation factor, putative s... 27 5.4 At1g62440.1 68414.m07044 leucine-rich repeat family protein / ex... 27 5.4 At1g22020.1 68414.m02755 glycine hydroxymethyltransferase, putat... 27 5.4 At4g28910.2 68417.m04132 expressed protein 27 7.1 At4g28910.1 68417.m04131 expressed protein 27 7.1 At4g36195.1 68417.m05150 serine carboxypeptidase S28 family prot... 27 9.4 At3g26320.1 68416.m03284 cytochrome P450 71B36, putative (CYP71B... 27 9.4 At1g35940.1 68414.m04463 AT hook motif-containing protein-relate... 27 9.4 >At2g02580.1 68415.m00198 cytochrome P450 family protein Length = 500 Score = 31.5 bits (68), Expect = 0.33 Identities = 12/34 (35%), Positives = 19/34 (55%) Frame = +2 Query: 17 LKLIFICAIVYYADAHKNNKRQTEQKPAEPDGPV 118 L L+F+C I+ A HK + +Q P+ P P+ Sbjct: 7 LSLLFLCCILLAAFKHKKRRTNQQQPPSPPGFPI 40 >At3g61220.1 68416.m06851 short-chain dehydrogenase/reductase (SDR) family protein similar to carbonyl reductase GI:1049108 from [Mus musculus] Length = 296 Score = 30.7 bits (66), Expect = 0.58 Identities = 14/44 (31%), Positives = 22/44 (50%) Frame = +2 Query: 269 RVDASKEGPACPLPVPPTYYVDEDCLRINVYTPSNNVSKPLPVI 400 R A KEG + TY + E+C++IN Y P +P++ Sbjct: 108 RAGAGKEGFKWDEIITETYELTEECIKINYYGPKRMCEAFIPLL 151 >At2g03550.1 68415.m00315 expressed protein similar to PrMC3 [Pinus radiata] GI:5487873; contains an esterase/lipase/thioesterase active site serine domain (prosite: PS50187) Length = 312 Score = 30.3 bits (65), Expect = 0.76 Identities = 13/37 (35%), Positives = 17/37 (45%) Frame = +2 Query: 335 EDCLRINVYTPSNNVSKPLPVIFYIHAGAFYSMTGRS 445 E L + +Y P K LP++ Y H G F T S Sbjct: 50 EKNLSLRIYLPEKVTVKKLPILIYFHGGGFIIETAFS 86 >At1g19190.1 68414.m02389 expressed protein contains similarity to anther-specific and pathogenesis response protein (PrMC3) GI:5487873 from [Pinus radiata] Length = 318 Score = 30.3 bits (65), Expect = 0.76 Identities = 15/46 (32%), Positives = 22/46 (47%), Gaps = 4/46 (8%) Frame = +2 Query: 326 YVDEDCLRINVYTPSNNV----SKPLPVIFYIHAGAFYSMTGRSDL 451 Y E L + +Y P N+V K +P++ Y H G F T S + Sbjct: 47 YSPEKNLSLRIYLPQNSVYETGEKKIPLLVYFHGGGFIMETAFSPI 92 >At4g37160.1 68417.m05261 multi-copper oxidase type I family protein contains Pfam profile: PF00394 Multicopper oxidase Length = 541 Score = 29.5 bits (63), Expect = 1.3 Identities = 14/38 (36%), Positives = 17/38 (44%) Frame = +2 Query: 218 VGKLRFQPPKLIRQYKERVDASKEGPACPLPVPPTYYV 331 V RF P L R SK PLP+ PTY++ Sbjct: 279 VASTRFTKPVLTTTASLRYQGSKNAAYGPLPIGPTYHI 316 >At2g24930.1 68415.m02981 Ulp1 protease family protein contains Pfam profile PF02902: Ulp1 protease family, C-terminal catalytic domain Length = 926 Score = 29.5 bits (63), Expect = 1.3 Identities = 19/74 (25%), Positives = 33/74 (44%), Gaps = 1/74 (1%) Frame = +2 Query: 71 NKRQTEQKPAEPDGPVTRSQSG-SFLGSWMETRRGKQFQAYRGIRYAEPPVGKLRFQPPK 247 N+R +KP P+ P + + + L TRRG+ + + P K+R PK Sbjct: 516 NRRLRSRKPPSPNVPKHKKEKTLNELIQKPPTRRGRGRKPLEQPKKVPPTALKIRINKPK 575 Query: 248 LIRQYKERVDASKE 289 + K + + +KE Sbjct: 576 PSEETKSKAEEAKE 589 >At2g24190.1 68415.m02890 short-chain dehydrogenase/reductase (SDR) family protein contains INTERPRO family IPR002198 short-chain dehydrogenase/reductase (SDR) superfamily Length = 296 Score = 29.5 bits (63), Expect = 1.3 Identities = 13/44 (29%), Positives = 21/44 (47%) Frame = +2 Query: 269 RVDASKEGPACPLPVPPTYYVDEDCLRINVYTPSNNVSKPLPVI 400 R KEG + TY + E+C++IN Y P +P++ Sbjct: 108 RAGTGKEGFKWEETITETYELAEECIKINYYGPKRMCEAFIPLL 151 >At1g76590.1 68414.m08912 zinc-binding family protein similar to zinc-binding protein [Pisum sativum] GI:16117799; contains Pfam profile PF04640 : Protein of unknown function, DUF597 Length = 245 Score = 28.7 bits (61), Expect = 2.3 Identities = 16/44 (36%), Positives = 21/44 (47%) Frame = -3 Query: 204 YLMPL*AWNCFPRRVSIHEPKNEPDCERVTGPSGSAGFCSVCLL 73 +L+P+ N F SIH N+ +C S FCS CLL Sbjct: 20 WLIPMLRANYFIP-CSIHAASNKSECNMFCLDCSSEAFCSYCLL 62 >At3g26300.1 68416.m03282 cytochrome P450 family protein contains Pfam profile: PF00067 cytochrome P450 Length = 500 Score = 27.5 bits (58), Expect = 5.4 Identities = 12/35 (34%), Positives = 17/35 (48%) Frame = +2 Query: 14 LLKLIFICAIVYYADAHKNNKRQTEQKPAEPDGPV 118 LL LIF+ I+ HKN + P+ P P+ Sbjct: 6 LLSLIFVICILVAVFNHKNRRNYQRTPPSPPGCPI 40 >At2g23630.1 68415.m02819 multi-copper oxidase type I family protein contains Pfam profile: PF00394 Multicopper oxidase Length = 541 Score = 27.5 bits (58), Expect = 5.4 Identities = 14/38 (36%), Positives = 18/38 (47%) Frame = +2 Query: 218 VGKLRFQPPKLIRQYKERVDASKEGPACPLPVPPTYYV 331 V RF P L SK P+ PLP+ PTY++ Sbjct: 277 VASTRFTKPILTTTGILSYQGSKIRPSHPLPIGPTYHI 314 >At2g17580.1 68415.m02034 polynucleotide adenylyltransferase family protein similar to SP|P13685 Poly(A) polymerase (EC 2.7.7.19) {Escherichia coli O157:H7}; contains Pfam profile PF01743: polyA polymerase family protein Length = 757 Score = 27.5 bits (58), Expect = 5.4 Identities = 14/41 (34%), Positives = 24/41 (58%) Frame = +2 Query: 56 DAHKNNKRQTEQKPAEPDGPVTRSQSGSFLGSWMETRRGKQ 178 D+ +NN ++TE P P GP ++Q S + ++ RR K+ Sbjct: 593 DSEQNNDQETEVCPRTPSGP-AKNQDQSVV-QMLKRRRSKE 631 >At1g71800.1 68414.m08298 cleavage stimulation factor, putative similar to cleavage stimulation factor 64 kilodalton subunit GB:AAD47839 GI:5713194 from [Drosophila melanogaster], SP|P33240 Cleavage stimulation factor, 64 kDa subunit {Homo sapiens}; contains InterPro entry IPR000504: RNA-binding region RNP-1 (RNA recognition motif) (RRM) Length = 461 Score = 27.5 bits (58), Expect = 5.4 Identities = 16/51 (31%), Positives = 26/51 (50%) Frame = +1 Query: 154 DGDTSRKAVPGL*GHKIR*ATGRETTIPAPKTNSAIQRTR*REQRGPGLPS 306 D +T+ A L ++I GR+ + + + +TR + Q GPGLPS Sbjct: 59 DEETALSARRNLQSYEIN---GRQLRVDFAENDKGTDKTRDQSQGGPGLPS 106 >At1g62440.1 68414.m07044 leucine-rich repeat family protein / extensin family protein similar to extensin-like protein [Lycopersicon esculentum] gi|5917664|gb|AAD55979; contains leucine-rich repeats, Pfam:PF00560; contains proline rich extensin domains, INTERPRO:IPR002965 Length = 826 Score = 27.5 bits (58), Expect = 5.4 Identities = 15/36 (41%), Positives = 17/36 (47%), Gaps = 1/36 (2%) Frame = +2 Query: 302 PLPVPPTYY-VDEDCLRINVYTPSNNVSKPLPVIFY 406 P P PPTYY V VY P S P P ++Y Sbjct: 619 PPPPPPTYYAVQSPPPPPPVYYPPVTASPPPPPVYY 654 >At1g22020.1 68414.m02755 glycine hydroxymethyltransferase, putative / serine hydroxymethyltransferase, putative / serine/threonine aldolase, putative similar to serine hydroxymethyltransferase [Chlamydomonas reinhardtii] GI:17066746; contains Pfam profile PF00464: serine hydroxymethyltransferase Length = 599 Score = 27.5 bits (58), Expect = 5.4 Identities = 12/37 (32%), Positives = 21/37 (56%), Gaps = 3/37 (8%) Frame = +2 Query: 296 ACPLPVPPTYYVDEDCLRINV---YTPSNNVSKPLPV 397 A PLP PP + +D + + + + PS+N P+P+ Sbjct: 18 ALPLPHPPRIPIADDSITLQIDSSFRPSSNPMPPVPL 54 >At4g28910.2 68417.m04132 expressed protein Length = 425 Score = 27.1 bits (57), Expect = 7.1 Identities = 13/27 (48%), Positives = 14/27 (51%) Frame = +2 Query: 56 DAHKNNKRQTEQKPAEPDGPVTRSQSG 136 D KN T Q+PAEP RS SG Sbjct: 50 DDFKNFLHPTSQRPAEPSSGSQRSDSG 76 >At4g28910.1 68417.m04131 expressed protein Length = 425 Score = 27.1 bits (57), Expect = 7.1 Identities = 13/27 (48%), Positives = 14/27 (51%) Frame = +2 Query: 56 DAHKNNKRQTEQKPAEPDGPVTRSQSG 136 D KN T Q+PAEP RS SG Sbjct: 50 DDFKNFLHPTSQRPAEPSSGSQRSDSG 76 >At4g36195.1 68417.m05150 serine carboxypeptidase S28 family protein contains Pfam PF05577: Serine carboxypeptidase S28 Length = 488 Score = 26.6 bits (56), Expect = 9.4 Identities = 13/56 (23%), Positives = 27/56 (48%) Frame = -2 Query: 451 QVAPARHGVERSGVNVKYHRERLRDIVTRSIHVDSQAVFVDVVSGRDGQRAGRALF 284 QVAPA + +N +YH + + + + ++ + A ++ G D A + +F Sbjct: 350 QVAPANDSIRSHQINTEYHLDLCKSLFGKGVYPEVDA--TNLYYGSDRIAATKIIF 403 >At3g26320.1 68416.m03284 cytochrome P450 71B36, putative (CYP71B36) identical to Cytochrome P450 71B36 (SP:Q9LIP4) [Arabidopsis thaliana]; contains Pfam profile: PF00067 cytochrome P450 Length = 500 Score = 26.6 bits (56), Expect = 9.4 Identities = 12/34 (35%), Positives = 20/34 (58%) Frame = +2 Query: 11 MLLKLIFICAIVYYADAHKNNKRQTEQKPAEPDG 112 + L L+F+ I+ A HK ++Q ++KP P G Sbjct: 5 LFLSLLFLSCILLAAFTHK-KRQQHQRKPPSPPG 37 >At1g35940.1 68414.m04463 AT hook motif-containing protein-related very low similarity to SP|Q9UUA2 DNA repair and recombination protein pif1, mitochondrial precursor {Schizosaccharomyces pombe}; contains weak hit to PF02178: AT hook motif Length = 1678 Score = 26.6 bits (56), Expect = 9.4 Identities = 14/49 (28%), Positives = 28/49 (57%), Gaps = 5/49 (10%) Frame = -2 Query: 394 RERLRDIVTRSIHVDSQAVFVDVVS-----GRDGQRAGRALFARVNAFF 263 + + D++ R +VDSQ + ++ G++G+RA L+A + A+F Sbjct: 1027 KSNIEDVLERVANVDSQFMAWLTLNRRNAVGKNGKRARECLYAEIPAYF 1075 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 11,506,337 Number of Sequences: 28952 Number of extensions: 256807 Number of successful extensions: 711 Number of sequences better than 10.0: 19 Number of HSP's better than 10.0 without gapping: 687 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 709 length of database: 12,070,560 effective HSP length: 76 effective length of database: 9,870,208 effective search space used: 888318720 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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