Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= brP-0316
(750 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g58420.1 68418.m07315 40S ribosomal protein S4 (RPS4D) riboso... 366 e-102
At5g07090.1 68418.m00804 40S ribosomal protein S4 (RPS4B) 366 e-101
At2g17360.1 68415.m02005 40S ribosomal protein S4 (RPS4A) contai... 366 e-101
At1g29760.1 68414.m03639 expressed protein 31 0.82
At1g15780.1 68414.m01893 expressed protein 31 1.1
At4g38440.1 68417.m05432 expressed protein 30 1.9
At4g10710.1 68417.m01751 transcriptional regulator-related simil... 30 1.9
At5g16910.1 68418.m01982 cellulose synthase family protein simil... 29 2.5
At3g03050.1 68416.m00301 cellulose synthase family protein (CslD... 29 3.3
At2g38050.1 68415.m04671 3-oxo-5-alpha-steroid 4-dehydrogenase, ... 29 3.3
At1g02730.1 68414.m00226 cellulose synthase family protein simil... 29 3.3
At5g63860.1 68418.m08016 UVB-resistance protein (UVR8) identical... 29 4.4
At4g08180.3 68417.m01353 oxysterol-binding family protein simila... 28 5.8
At4g08180.2 68417.m01352 oxysterol-binding family protein simila... 28 5.8
At4g08180.1 68417.m01351 oxysterol-binding family protein simila... 28 5.8
At4g38190.1 68417.m05391 cellulose synthase family protein simil... 28 7.6
At2g26730.1 68415.m03206 leucine-rich repeat transmembrane prote... 28 7.6
>At5g58420.1 68418.m07315 40S ribosomal protein S4 (RPS4D) ribosomal
protein S4, Arabidopsis thaliana, PIR:T48480
Length = 262
Score = 366 bits (901), Expect = e-102
Identities = 166/236 (70%), Positives = 198/236 (83%)
Frame = +3
Query: 3 LKRLNAPKHGMLDKLGGVYAPRPSTGPHKLRECLPLVIFLRNRLKYALTGNEVLKIVKQR 182
LKRLNAPKH MLDKLGG +AP+PS+GPHK RECLPLV+ +RNRLKYALT EV+ I+ QR
Sbjct: 9 LKRLNAPKHWMLDKLGGAFAPKPSSGPHKSRECLPLVLIIRNRLKYALTYREVISILMQR 68
Query: 183 LIKVDGKVRTDPTYPAGFMDVVSIEKTNELFRLIYDVKGRFTIHRITPEEAKYKLCKVKR 362
I+VDGKVRTD TYPAGFMDVVSI KTNE FRL+YD KGRF +H I EEAK+KLCKV+
Sbjct: 69 HIQVDGKVRTDKTYPAGFMDVVSIPKTNENFRLLYDTKGRFRLHSIKDEEAKFKLCKVRS 128
Query: 363 VATGPKNVPYLVTHDGRTIRYPDPLIKVNDSIQLDIATTKIMDFIKFESGNLCMITGGRN 542
+ G K +PYL T+DGRTIRYPDPLIK ND+I+LD+ KI++FIKF+ GN+ M+TGGRN
Sbjct: 129 IQFGQKGIPYLNTYDGRTIRYPDPLIKPNDTIKLDLEANKIVEFIKFDVGNVVMVTGGRN 188
Query: 543 LGRVGTIVSRERHPGSFDIVHIKDSTGHTFATRLNNVFIIGKGTKAYISLPRGKGI 710
GRVG I +RE+H GSF+ +HI+DSTGH FATRL NV+ IGKGTK ++SLP+GKGI
Sbjct: 189 RGRVGVIKNREKHKGSFETIHIQDSTGHEFATRLGNVYTIGKGTKPWVSLPKGKGI 244
>At5g07090.1 68418.m00804 40S ribosomal protein S4 (RPS4B)
Length = 262
Score = 366 bits (900), Expect = e-101
Identities = 166/236 (70%), Positives = 198/236 (83%)
Frame = +3
Query: 3 LKRLNAPKHGMLDKLGGVYAPRPSTGPHKLRECLPLVIFLRNRLKYALTGNEVLKIVKQR 182
LKRLNAPKH MLDKLGG +AP+PS+GPHK RECLPLV+ +RNRLKYALT EV+ I+ QR
Sbjct: 9 LKRLNAPKHWMLDKLGGAFAPKPSSGPHKSRECLPLVLIIRNRLKYALTYREVISILMQR 68
Query: 183 LIKVDGKVRTDPTYPAGFMDVVSIEKTNELFRLIYDVKGRFTIHRITPEEAKYKLCKVKR 362
I+VDGKVRTD TYPAGFMDVVSI KTNE FRL+YD KGRF +H I EEAK+KLCKV+
Sbjct: 69 HIQVDGKVRTDKTYPAGFMDVVSIPKTNENFRLLYDTKGRFRLHSIKDEEAKFKLCKVRS 128
Query: 363 VATGPKNVPYLVTHDGRTIRYPDPLIKVNDSIQLDIATTKIMDFIKFESGNLCMITGGRN 542
+ G K +PYL T+DGRTIRYPDPLIK ND+I+LD+ KI++FIKF+ GN+ M+TGGRN
Sbjct: 129 IQFGQKGIPYLNTYDGRTIRYPDPLIKPNDTIKLDLEENKIVEFIKFDVGNVVMVTGGRN 188
Query: 543 LGRVGTIVSRERHPGSFDIVHIKDSTGHTFATRLNNVFIIGKGTKAYISLPRGKGI 710
GRVG I +RE+H GSF+ +HI+DSTGH FATRL NV+ IGKGTK ++SLP+GKGI
Sbjct: 189 RGRVGVIKNREKHKGSFETIHIQDSTGHEFATRLGNVYTIGKGTKPWVSLPKGKGI 244
>At2g17360.1 68415.m02005 40S ribosomal protein S4 (RPS4A) contains
ribosomal protein S4 signature from residues 8 to 22
Length = 261
Score = 366 bits (900), Expect = e-101
Identities = 166/236 (70%), Positives = 198/236 (83%)
Frame = +3
Query: 3 LKRLNAPKHGMLDKLGGVYAPRPSTGPHKLRECLPLVIFLRNRLKYALTGNEVLKIVKQR 182
LKRLNAPKH MLDKLGG +AP+PS+GPHK RECLPLV+ +RNRLKYALT EV+ I+ QR
Sbjct: 9 LKRLNAPKHWMLDKLGGAFAPKPSSGPHKSRECLPLVLIIRNRLKYALTYREVISILMQR 68
Query: 183 LIKVDGKVRTDPTYPAGFMDVVSIEKTNELFRLIYDVKGRFTIHRITPEEAKYKLCKVKR 362
I+VDGKVRTD TYPAGFMDVVSI KTNE FRL+YD KGRF +H I EEAK+KLCKV+
Sbjct: 69 HIQVDGKVRTDKTYPAGFMDVVSIPKTNENFRLLYDTKGRFRLHSIKDEEAKFKLCKVRS 128
Query: 363 VATGPKNVPYLVTHDGRTIRYPDPLIKVNDSIQLDIATTKIMDFIKFESGNLCMITGGRN 542
+ G K +PYL T+DGRTIRYPDPLIK ND+I+LD+ KI++FIKF+ GN+ M+TGGRN
Sbjct: 129 IQFGQKGIPYLNTYDGRTIRYPDPLIKPNDTIKLDLEENKIVEFIKFDVGNVVMVTGGRN 188
Query: 543 LGRVGTIVSRERHPGSFDIVHIKDSTGHTFATRLNNVFIIGKGTKAYISLPRGKGI 710
GRVG I +RE+H GSF+ +HI+DSTGH FATRL NV+ IGKGTK ++SLP+GKGI
Sbjct: 189 RGRVGVIKNREKHKGSFETIHIQDSTGHEFATRLGNVYTIGKGTKPWVSLPKGKGI 244
>At1g29760.1 68414.m03639 expressed protein
Length = 526
Score = 31.1 bits (67), Expect = 0.82
Identities = 14/31 (45%), Positives = 17/31 (54%)
Frame = -3
Query: 214 SVLTFPSTFMRRCFTIFRTSFPVKAYFRRFL 122
S+LTFP +R CF F F + RRFL
Sbjct: 195 SLLTFPPWLLRNCFLFFFDPFSTIRFGRRFL 225
>At1g15780.1 68414.m01893 expressed protein
Length = 1335
Score = 30.7 bits (66), Expect = 1.1
Identities = 13/43 (30%), Positives = 25/43 (58%)
Frame = -2
Query: 716 RRNALAARQRDVRLRALADYEHVVQPRGEGVSRGVLDVHNVEG 588
++ L ARQ+ +L+ + D + +G VSRG+ H+++G
Sbjct: 861 QQQQLQARQQAAQLQQMNDMNDLTSRQGMNVSRGMFQQHSMQG 903
>At4g38440.1 68417.m05432 expressed protein
Length = 1465
Score = 29.9 bits (64), Expect = 1.9
Identities = 25/104 (24%), Positives = 45/104 (43%), Gaps = 4/104 (3%)
Frame = +3
Query: 6 KRLNAPKHGMLDKLGGVYAPRPSTGPHKLRECLPL----VIFLRNRLKYALTGNEVLKIV 173
+R+ P H L + V++ + STGP + E L + V FL + G+ +V
Sbjct: 1140 QRMPLPPHWFLSAISAVHSGKTSTGPPESTELLEVAKAGVFFLAGLESSSGFGSLPSPVV 1199
Query: 174 KQRLIKVDGKVRTDPTYPAGFMDVVSIEKTNELFRLIYDVKGRF 305
L+ K T MD++ + T L+ + ++ G+F
Sbjct: 1200 SVPLV---WKFHALSTVLLVGMDIIEDKNTRNLYNYLQELYGQF 1240
>At4g10710.1 68417.m01751 transcriptional regulator-related similar
to chromatin-specific transcription elongation factor
FACT 140 kDa subunit (GI:5499741) [Homo sapiens]
Length = 1074
Score = 29.9 bits (64), Expect = 1.9
Identities = 11/20 (55%), Positives = 13/20 (65%)
Frame = -3
Query: 103 KHSRNLWGPVDGLGAYTPPS 44
KHS +LWG D L TPP+
Sbjct: 44 KHSADLWGSADALAIATPPA 63
>At5g16910.1 68418.m01982 cellulose synthase family protein similar
to gi:2827143 cellulose synthase catalytic subunit,
Arabidopsis thaliana, gi:9622886 cellulose synthase-7
from Zea mays
Length = 1145
Score = 29.5 bits (63), Expect = 2.5
Identities = 13/32 (40%), Positives = 19/32 (59%)
Frame = -3
Query: 670 PLPIMNTLFNLVAKVCPVESLMCTMSKEPGCL 575
PL NT+ +++A PVE L C +S + G L
Sbjct: 395 PLVTANTILSILAAEYPVEKLSCYVSDDGGAL 426
>At3g03050.1 68416.m00301 cellulose synthase family protein (CslD3)
similar to cellulose synthase catalytic subunit
gi:2827143 from [Arabidopsis thaliana], cellulose
synthase-7 (gi:9622886) from Zea mays; contains Pfam
profile PF03552: Cellulose synthase
Length = 1145
Score = 29.1 bits (62), Expect = 3.3
Identities = 13/32 (40%), Positives = 19/32 (59%)
Frame = -3
Query: 670 PLPIMNTLFNLVAKVCPVESLMCTMSKEPGCL 575
PL NT+ +++A PVE L C +S + G L
Sbjct: 392 PLVTSNTILSILAADYPVEKLACYVSDDGGAL 423
>At2g38050.1 68415.m04671 3-oxo-5-alpha-steroid 4-dehydrogenase,
putative / steroid 5-alpha-reductase, putative identical
to gi:1280611; contains a steroid 5-alpha reductase,
C-terminal domain
Length = 262
Score = 29.1 bits (62), Expect = 3.3
Identities = 15/39 (38%), Positives = 19/39 (48%)
Frame = -3
Query: 193 TFMRRCFTIFRTSFPVKAYFRRFLRKITRGKHSRNLWGP 77
TF R C + P A +FL+ GKH+R WGP
Sbjct: 8 TFFRYCLLTLIFAGPPTAVLLKFLQA-PYGKHNRTGWGP 45
>At1g02730.1 68414.m00226 cellulose synthase family protein similar
to cellulose synthase catalytic subunit [gi:13925881]
from Nicotiana alata, cellulose synthase-4 [gi:9622880]
from Zea mays
Length = 1181
Score = 29.1 bits (62), Expect = 3.3
Identities = 13/32 (40%), Positives = 19/32 (59%)
Frame = -3
Query: 670 PLPIMNTLFNLVAKVCPVESLMCTMSKEPGCL 575
PL NT+ +++A PVE L C +S + G L
Sbjct: 416 PLVTANTILSILAVDYPVEKLACYLSDDGGAL 447
>At5g63860.1 68418.m08016 UVB-resistance protein (UVR8) identical to
UVB-resistance protein UVR8 (GI:5478530, GB:AAD43920.1)
[Arabidopsis thaliana]; contains Pfam 00415: Regulator
of chromosome condensation (RCC1)
Length = 440
Score = 28.7 bits (61), Expect = 4.4
Identities = 17/54 (31%), Positives = 25/54 (46%), Gaps = 1/54 (1%)
Frame = +3
Query: 534 GRNLGRVGTIVSRERHPGSFDIVHIKDSTGHTFA-TRLNNVFIIGKGTKAYISL 692
G NL + + + R P +V + HT A T NNVF G+GT + +
Sbjct: 311 GNNLDQCSPV--QVRFPDDQKVVQVSCGWRHTLAVTERNNVFAWGRGTNGQLGI 362
>At4g08180.3 68417.m01353 oxysterol-binding family protein similar
to SP|Q969R2 Oxysterol-binding protein 2 {Homo sapiens};
contains Pfam profiles PF00169: PH domain, PF01237:
Oxysterol-binding protein
Length = 813
Score = 28.3 bits (60), Expect = 5.8
Identities = 15/46 (32%), Positives = 26/46 (56%), Gaps = 1/46 (2%)
Frame = +3
Query: 126 NRLKYALTGNEVLKIVKQRLIKVDGKVRTDPTY-PAGFMDVVSIEK 260
N ++A+T NE+ +K+RL D ++R D Y G ++ + EK
Sbjct: 703 NLTRFAITLNELTPGLKERLPPTDSRLRPDQRYLENGEFEMANTEK 748
>At4g08180.2 68417.m01352 oxysterol-binding family protein similar
to SP|Q969R2 Oxysterol-binding protein 2 {Homo sapiens};
contains Pfam profiles PF00169: PH domain, PF01237:
Oxysterol-binding protein
Length = 813
Score = 28.3 bits (60), Expect = 5.8
Identities = 15/46 (32%), Positives = 26/46 (56%), Gaps = 1/46 (2%)
Frame = +3
Query: 126 NRLKYALTGNEVLKIVKQRLIKVDGKVRTDPTY-PAGFMDVVSIEK 260
N ++A+T NE+ +K+RL D ++R D Y G ++ + EK
Sbjct: 703 NLTRFAITLNELTPGLKERLPPTDSRLRPDQRYLENGEFEMANTEK 748
>At4g08180.1 68417.m01351 oxysterol-binding family protein similar
to SP|Q969R2 Oxysterol-binding protein 2 {Homo sapiens};
contains Pfam profiles PF00169: PH domain, PF01237:
Oxysterol-binding protein
Length = 814
Score = 28.3 bits (60), Expect = 5.8
Identities = 15/46 (32%), Positives = 26/46 (56%), Gaps = 1/46 (2%)
Frame = +3
Query: 126 NRLKYALTGNEVLKIVKQRLIKVDGKVRTDPTY-PAGFMDVVSIEK 260
N ++A+T NE+ +K+RL D ++R D Y G ++ + EK
Sbjct: 704 NLTRFAITLNELTPGLKERLPPTDSRLRPDQRYLENGEFEMANTEK 749
>At4g38190.1 68417.m05391 cellulose synthase family protein similar
to cellulose synthase catalytic subunit gi:2827143 from
[Arabidopsis thaliana], cellulose synthase-5
(gi:9622882) from Zea mays
Length = 1111
Score = 27.9 bits (59), Expect = 7.6
Identities = 12/32 (37%), Positives = 19/32 (59%)
Frame = -3
Query: 670 PLPIMNTLFNLVAKVCPVESLMCTMSKEPGCL 575
PL NT+ +++A PVE + C +S + G L
Sbjct: 370 PLVTANTILSILAVDYPVEKVSCYLSDDGGAL 401
>At2g26730.1 68415.m03206 leucine-rich repeat transmembrane protein
kinase, putative
Length = 658
Score = 27.9 bits (59), Expect = 7.6
Identities = 13/44 (29%), Positives = 23/44 (52%)
Frame = +3
Query: 402 HDGRTIRYPDPLIKVNDSIQLDIATTKIMDFIKFESGNLCMITG 533
H+ + +P ++N+ I+LDI++ I F NL +TG
Sbjct: 124 HNEFSGEFPTSFTQLNNLIRLDISSNNFTGSIPFSVNNLTHLTG 167
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 17,711,155
Number of Sequences: 28952
Number of extensions: 402000
Number of successful extensions: 1271
Number of sequences better than 10.0: 17
Number of HSP's better than 10.0 without gapping: 1206
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1269
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1663169840
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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