BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= brP-0312 (750 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC16H5.08c |||ribosome biogenesis ATPase, Arb family |Schizosa... 125 6e-30 SPBC29A3.09c |||AAA family ATPase Gcn20 |Schizosaccharomyces pom... 103 2e-23 SPCC825.01 |||ribosome biogenesis ATPase, Arb family |Schizosacc... 99 6e-22 SPAC3C7.08c |elf1||AAA family ATPase ELf1|Schizosaccharomyces po... 53 5e-08 SPCC417.08 |tef3||translation elongation factor eEF3|Schizosacch... 50 5e-07 SPAC20G4.01 ||SPAC22F8.13|CCR4-Not complex subunit Caf16|Schizos... 37 0.003 SPAC323.04 |||mitochondrial ATPase |Schizosaccharomyces pombe|ch... 34 0.019 SPBC359.05 |abc3||ABC transporter Abc3|Schizosaccharomyces pombe... 29 0.94 SPAC3F10.11c |abc2||glutathione S-conjugate-exporting ATPase Abc... 28 1.2 SPAC15A10.01 |atm1|SPAC8C9.18|ABC family iron transporter Atm1|S... 28 1.6 SPAC222.06 |mak16||nuclear HMG-like acidic protein Mak16|Schizos... 27 2.2 SPBC25B2.02c |mam1|SPBC2G5.09c|M-factor transporter Mam1 |Schizo... 27 2.2 SPBC14F5.06 |||iron-sulfur protein|Schizosaccharomyces pombe|chr... 27 2.9 SPCC737.03c |||conserved eukaryotic protein|Schizosaccharomyces ... 26 6.6 SPAC1296.04 |mug65||spore wall assembly protein |Schizosaccharom... 26 6.6 SPAP8A3.11c |||mitochondrial GTPase Mtg2|Schizosaccharomyces pom... 26 6.6 SPBC23E6.07c |rfc1||DNA replication factor C complex subunit Rfc... 26 6.6 SPAC9E9.14 |vps24||vacuolar sorting protein Vps24|Schizosaccharo... 26 6.6 SPAC1556.01c |rad50|SPAP4C9.01c|DNA repair protein Rad50|Schizos... 25 8.7 SPAC4F10.13c |mpd2||GYF domain|Schizosaccharomyces pombe|chr 1||... 25 8.7 >SPBC16H5.08c |||ribosome biogenesis ATPase, Arb family |Schizosaccharomyces pombe|chr 2|||Manual Length = 618 Score = 125 bits (302), Expect = 6e-30 Identities = 67/152 (44%), Positives = 96/152 (63%), Gaps = 1/152 (0%) Frame = +2 Query: 236 RSCTGSLAVHPRSRDIKIANFSITFYGSELLQDTLLELNCGRRYGLVGLNGCGKSSLLAA 415 RS +G L P SRDIKI +++++F+G L+++ +ELN G+RYGL+G NG GKS+ L + Sbjct: 61 RSASGVLTSQPMSRDIKIDSYTLSFHGRLLIENATIELNHGQRYGLLGDNGSGKSTFLES 120 Query: 416 LGRREVPIPEHIDIFHLTREMPASDKTALQCVMEVDEERV-KLEKLAEMLAHCEDDESQE 592 + R+V PEHID + L E SD A+ +++ +++V KLE E L+ DD Sbjct: 121 VAARDVEYPEHIDSYLLNAEAEPSDVNAVDYIIQSAKDKVQKLEAEIEELS-TADDVDDV 179 Query: 593 QLMDVYDRLDDLSADTAEARAANILHGLGFXQ 688 L Y+ LDD+ T EA+AA ILHGLGF Q Sbjct: 180 LLESKYEELDDMDPSTFEAKAAMILHGLGFTQ 211 Score = 27.9 bits (59), Expect = 1.6 Identities = 14/43 (32%), Positives = 23/43 (53%) Frame = +2 Query: 323 LLQDTLLELNCGRRYGLVGLNGCGKSSLLAALGRREVPIPEHI 451 L +D ++ R +VG NG GKS+LL + +PI ++ Sbjct: 405 LYRDLSFGIDMDSRVAIVGKNGTGKSTLLNLITGLLIPIEGNV 447 >SPBC29A3.09c |||AAA family ATPase Gcn20 |Schizosaccharomyces pombe|chr 2|||Manual Length = 736 Score = 103 bits (248), Expect = 2e-23 Identities = 67/167 (40%), Positives = 91/167 (54%), Gaps = 27/167 (16%) Frame = +2 Query: 269 RSRDIKIANFSITFYGSELLQDTLLELNCGRRYGLVGLNGCGKSSLLAALGRREVPIPEH 448 +S+DIKI + F G +L L L GRRYGL G NG GKS+LL AL RRE+ IP H Sbjct: 179 KSKDIKIDGIDLAFAGHRILTGASLTLAQGRRYGLTGRNGIGKSTLLRALSRREIAIPTH 238 Query: 449 IDIFHLTREMPASDKTALQCVMEV---------DEERV-----KLEKLAEMLA------- 565 I I H+ +EM D ALQ V++ D+E++ +EK E L+ Sbjct: 239 ITILHVEQEMTGDDTPALQSVLDADVWRKYLIQDQEKITNRLSTIEKELEELSKDQTADQ 298 Query: 566 ------HCEDDESQEQLMDVYDRLDDLSADTAEARAANILHGLGFXQ 688 E DE +L+D+ ++L ++ +D AE+RAA IL GLGF Q Sbjct: 299 AISRRLERERDELDLRLLDIQNKLSEMDSDRAESRAATILAGLGFTQ 345 Score = 29.1 bits (62), Expect = 0.71 Identities = 13/35 (37%), Positives = 21/35 (60%) Frame = +2 Query: 314 GSELLQDTLLELNCGRRYGLVGLNGCGKSSLLAAL 418 G +L+ +++ R G+VG NG GKS++L L Sbjct: 530 GHPILKHVDIDVQMDSRIGVVGPNGAGKSTMLKLL 564 >SPCC825.01 |||ribosome biogenesis ATPase, Arb family |Schizosaccharomyces pombe|chr 3|||Manual Length = 822 Score = 99.1 bits (236), Expect = 6e-22 Identities = 54/147 (36%), Positives = 87/147 (59%), Gaps = 1/147 (0%) Frame = +2 Query: 245 TGSLAVHPRSRDIKIANFSITFYGSELLQDTLLELNCGRRYGLVGLNGCGKSSLLAALGR 424 TG+L P SRD+++ S++ +G L++D+ L L GRRYGL+ NG GKS+LL A+ Sbjct: 264 TGNLLSPPNSRDLQVEKLSVSAWGKLLIKDSELNLINGRRYGLIAPNGSGKSTLLHAIAC 323 Query: 425 REVPIPEHIDIFHLTREMPASDKTALQCVMEVDE-ERVKLEKLAEMLAHCEDDESQEQLM 601 +P P +D + L RE ++ T ++ V++++E ER LE + E L + D++ +L Sbjct: 324 GLIPTPSSLDFYLLDREYIPNELTCVEAVLDINEQERKHLEAMMEDLLD-DPDKNAVELD 382 Query: 602 DVYDRLDDLSADTAEARAANILHGLGF 682 + RL DL + ++ R IL GL F Sbjct: 383 TIQTRLTDLETENSDHRVYKILRGLQF 409 Score = 25.4 bits (53), Expect = 8.7 Identities = 16/53 (30%), Positives = 25/53 (47%), Gaps = 1/53 (1%) Frame = +2 Query: 281 IKIANFSITFYGSELLQDTL-LELNCGRRYGLVGLNGCGKSSLLAALGRREVP 436 IK + S + G + L L+ R LVG NG GK++L+ + + P Sbjct: 594 IKFQDVSFNYPGGPTIFSKLNFGLDLKSRVALVGPNGAGKTTLIKLILEKVQP 646 >SPAC3C7.08c |elf1||AAA family ATPase ELf1|Schizosaccharomyces pombe|chr 1|||Manual Length = 1057 Score = 52.8 bits (121), Expect = 5e-08 Identities = 28/69 (40%), Positives = 39/69 (56%), Gaps = 3/69 (4%) Frame = +2 Query: 293 NFSITFYGSELLQDTLLELNCGRRYGLVGLNGCGKSSLLAALGRREV---PIPEHIDIFH 463 +FS+ + G LL T L L G RYG+VG NGCGKS+LL A+G +V P P+ + Sbjct: 449 DFSLAYGGRLLLSHTNLHLYRGHRYGVVGHNGCGKSTLLRAIGDYKVENFPSPDEVKTCF 508 Query: 464 LTREMPASD 490 + + D Sbjct: 509 VAHSLQGED 517 Score = 29.1 bits (62), Expect = 0.71 Identities = 16/54 (29%), Positives = 29/54 (53%), Gaps = 2/54 (3%) Frame = +2 Query: 281 IKIANFSITFYGSEL--LQDTLLELNCGRRYGLVGLNGCGKSSLLAALGRREVP 436 +K+ N S T+ ++ L + + L+ R ++G NG GKS+L+ L +P Sbjct: 692 LKMTNASYTYPNAKKKSLDNVTVGLSLSSRVAILGPNGAGKSTLIKVLIGEVIP 745 >SPCC417.08 |tef3||translation elongation factor eEF3|Schizosaccharomyces pombe|chr 3|||Manual Length = 1047 Score = 49.6 bits (113), Expect = 5e-07 Identities = 29/111 (26%), Positives = 56/111 (50%), Gaps = 1/111 (0%) Frame = +2 Query: 278 DIKIANFSITFYGSELLQDTLLELNCGRRYGLVGLNGCGKSSLLAALGRREVP-IPEHID 454 D+ FS+ + LL T L L GRRYGL G NG GKS+L+ A+ +V P H+ Sbjct: 434 DLCNCEFSLAYGAKILLNRTRLRLKRGRRYGLCGPNGSGKSTLMRAIVNGQVEGFPTHLR 493 Query: 455 IFHLTREMPASDKTALQCVMEVDEERVKLEKLAEMLAHCEDDESQEQLMDV 607 ++ ++ S+ + + V ++ E++ +++ ++L+++ Sbjct: 494 TVYVEHDIDESEADTPSVDFILQDPAVPIKDRDEIVKALKENSFTDELINM 544 Score = 30.3 bits (65), Expect = 0.31 Identities = 16/54 (29%), Positives = 28/54 (51%), Gaps = 2/54 (3%) Frame = +2 Query: 281 IKIANFSITFYGSEL--LQDTLLELNCGRRYGLVGLNGCGKSSLLAALGRREVP 436 IK+ + S + G+ L D +++ R ++G NG GKS+L+ L +P Sbjct: 673 IKVQHMSFQYPGTSKPQLNDISFQVSLSSRIAVIGPNGAGKSTLIKVLTGELLP 726 >SPAC20G4.01 ||SPAC22F8.13|CCR4-Not complex subunit Caf16|Schizosaccharomyces pombe|chr 1|||Manual Length = 280 Score = 37.1 bits (82), Expect = 0.003 Identities = 22/62 (35%), Positives = 32/62 (51%), Gaps = 2/62 (3%) Frame = +2 Query: 278 DIKIANFSITFYGSE--LLQDTLLELNCGRRYGLVGLNGCGKSSLLAALGRREVPIPEHI 451 ++ ++N S TF + L L+L G R LVG NG GKS+LL L + + HI Sbjct: 2 EVTVSNLSYTFSPKQPLSLDHVTLDLPKGSRTLLVGANGAGKSTLLKLLSGKSLAKAGHI 61 Query: 452 DI 457 + Sbjct: 62 SV 63 >SPAC323.04 |||mitochondrial ATPase |Schizosaccharomyces pombe|chr 1|||Manual Length = 487 Score = 34.3 bits (75), Expect = 0.019 Identities = 15/44 (34%), Positives = 26/44 (59%) Frame = +2 Query: 281 IKIANFSITFYGSELLQDTLLELNCGRRYGLVGLNGCGKSSLLA 412 I + + + ++G ++L D + G R+ L G NG GK++LLA Sbjct: 265 ISMEHLNCVYWGRKVLSDINWTIREGERWALTGSNGSGKTTLLA 308 >SPBC359.05 |abc3||ABC transporter Abc3|Schizosaccharomyces pombe|chr 2|||Manual Length = 1465 Score = 28.7 bits (61), Expect = 0.94 Identities = 16/44 (36%), Positives = 24/44 (54%) Frame = +2 Query: 326 LQDTLLELNCGRRYGLVGLNGCGKSSLLAALGRREVPIPEHIDI 457 L + +E++ + G+VG G GKS+L AL R P I+I Sbjct: 1243 LNNINIEISPREKIGIVGRTGAGKSTLAMALFRIIEPTEGKIEI 1286 >SPAC3F10.11c |abc2||glutathione S-conjugate-exporting ATPase Abc2|Schizosaccharomyces pombe|chr 1|||Manual Length = 1463 Score = 28.3 bits (60), Expect = 1.2 Identities = 19/66 (28%), Positives = 31/66 (46%), Gaps = 2/66 (3%) Frame = +2 Query: 266 PRSRDIKIANFSITFYGSE--LLQDTLLELNCGRRYGLVGLNGCGKSSLLAALGRREVPI 439 P IK ++S+ + + +L D + + + G+VG G GKS+L AL R P Sbjct: 1219 PSHGAIKFDHYSVRYRENLPLVLNDISVNIKPQEKIGIVGRTGAGKSTLTLALFRLIEPT 1278 Query: 440 PEHIDI 457 I + Sbjct: 1279 SGDIQL 1284 Score = 26.6 bits (56), Expect = 3.8 Identities = 21/56 (37%), Positives = 29/56 (51%), Gaps = 5/56 (8%) Frame = +2 Query: 278 DIKIANFSITFYGSELLQDTLLELNCGRRYG----LVGLNGCGKSSLL-AALGRRE 430 +IK FS + G + TL +++ R G +VG G GKSSLL A LG + Sbjct: 579 EIKKGTFSWSGPGQNAAEPTLRDIDFVARRGELCCIVGKVGMGKSSLLEACLGNMQ 634 >SPAC15A10.01 |atm1|SPAC8C9.18|ABC family iron transporter Atm1|Schizosaccharomyces pombe|chr 1|||Manual Length = 693 Score = 27.9 bits (59), Expect = 1.6 Identities = 12/34 (35%), Positives = 18/34 (52%) Frame = +2 Query: 323 LLQDTLLELNCGRRYGLVGLNGCGKSSLLAALGR 424 +L + G + VG +GCGKS++L L R Sbjct: 458 ILNGCSFNIPAGAKVAFVGASGCGKSTILRLLFR 491 >SPAC222.06 |mak16||nuclear HMG-like acidic protein Mak16|Schizosaccharomyces pombe|chr 1|||Manual Length = 302 Score = 27.5 bits (58), Expect = 2.2 Identities = 15/45 (33%), Positives = 25/45 (55%), Gaps = 1/45 (2%) Frame = +2 Query: 518 VDEERVKLEKLAEMLAHCEDDESQEQLMDVYDRL-DDLSADTAEA 649 +DE + E+ L DDE +E++ D+ D L D S +T+E+ Sbjct: 197 IDEGEEEEEREEAELEFVSDDEDEEEISDLEDWLGSDQSMETSES 241 >SPBC25B2.02c |mam1|SPBC2G5.09c|M-factor transporter Mam1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 1336 Score = 27.5 bits (58), Expect = 2.2 Identities = 16/49 (32%), Positives = 28/49 (57%), Gaps = 1/49 (2%) Frame = +2 Query: 326 LQDTLLELNCGRRYGLVGLNGCGKSSLLAALGRREVPIPE-HIDIFHLT 469 L + L + + +VG++G GKS+L+ L R+ P + +ID + LT Sbjct: 1118 LNNVSLSIEAREKVAIVGISGSGKSTLVELL-RKTYPSEDIYIDGYPLT 1165 >SPBC14F5.06 |||iron-sulfur protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 593 Score = 27.1 bits (57), Expect = 2.9 Identities = 13/27 (48%), Positives = 16/27 (59%) Frame = +2 Query: 356 GRRYGLVGLNGCGKSSLLAALGRREVP 436 G+ GLVG NG GKS+ L L + P Sbjct: 103 GQVLGLVGTNGIGKSTALKILSGKMKP 129 >SPCC737.03c |||conserved eukaryotic protein|Schizosaccharomyces pombe|chr 3|||Manual Length = 615 Score = 25.8 bits (54), Expect = 6.6 Identities = 15/35 (42%), Positives = 21/35 (60%) Frame = -3 Query: 394 STPIKSD*PISSPTIQLQESILK*LTPIESN*KVS 290 S+P++ P+ PT+QLQ S L L P E KV+ Sbjct: 437 SSPVR---PMLKPTLQLQNSPLSKLVPQEVGNKVN 468 >SPAC1296.04 |mug65||spore wall assembly protein |Schizosaccharomyces pombe|chr 1|||Manual Length = 248 Score = 25.8 bits (54), Expect = 6.6 Identities = 10/32 (31%), Positives = 11/32 (34%) Frame = +1 Query: 649 ACC*YPAWSWLXPRECXRRQPKTSSGGWPYAF 744 A C P+W W R GW Y F Sbjct: 56 ATCPDPSWEWAWQRWYIDMSGDVDESGWQYGF 87 >SPAP8A3.11c |||mitochondrial GTPase Mtg2|Schizosaccharomyces pombe|chr 1|||Manual Length = 419 Score = 25.8 bits (54), Expect = 6.6 Identities = 15/36 (41%), Positives = 19/36 (52%) Frame = +2 Query: 341 LELNCGRRYGLVGLNGCGKSSLLAALGRREVPIPEH 448 LEL GLVGL GKS+LL L + + E+ Sbjct: 231 LELKTICEIGLVGLPNAGKSTLLNCLTASKSKVGEY 266 >SPBC23E6.07c |rfc1||DNA replication factor C complex subunit Rfc1|Schizosaccharomyces pombe|chr 2|||Manual Length = 934 Score = 25.8 bits (54), Expect = 6.6 Identities = 12/57 (21%), Positives = 26/57 (45%) Frame = +2 Query: 422 RREVPIPEHIDIFHLTREMPASDKTALQCVMEVDEERVKLEKLAEMLAHCEDDESQE 592 + ++P +F+L +PAS T ++ + +K+ E +A +D +E Sbjct: 298 QHKIPAINEDGLFYLITHLPASGGTGAAAQAAQQKKEQEEKKILETVARMDDSNKKE 354 >SPAC9E9.14 |vps24||vacuolar sorting protein Vps24|Schizosaccharomyces pombe|chr 1|||Manual Length = 231 Score = 25.8 bits (54), Expect = 6.6 Identities = 16/54 (29%), Positives = 27/54 (50%), Gaps = 1/54 (1%) Frame = +2 Query: 476 MPA-SDKTALQCVMEVDEERVKLEKLAEMLAHCEDDESQEQLMDVYDRLDDLSA 634 +PA S+K A + L L+ +A + +EQL+D+ D+LD L + Sbjct: 178 IPAPSEKAADAATHREQSLKQALPSLSNGIAKDSTEIDEEQLLDIRDKLDALKS 231 >SPAC1556.01c |rad50|SPAP4C9.01c|DNA repair protein Rad50|Schizosaccharomyces pombe|chr 1|||Manual Length = 1290 Score = 25.4 bits (53), Expect = 8.7 Identities = 17/60 (28%), Positives = 30/60 (50%), Gaps = 1/60 (1%) Frame = +2 Query: 467 TREMPASDKTALQCVMEVDEERVKLEK-LAEMLAHCEDDESQEQLMDVYDRLDDLSADTA 643 TRE ASD L+ +M EE V + L +H + + +E++ + + L+A+ A Sbjct: 1000 TRERNASDNLRLRALMRQLEEAVTQKNYLLSQQSHDDRESFRERMQILKSKYGALNAERA 1059 >SPAC4F10.13c |mpd2||GYF domain|Schizosaccharomyces pombe|chr 1|||Manual Length = 992 Score = 25.4 bits (53), Expect = 8.7 Identities = 11/28 (39%), Positives = 18/28 (64%) Frame = -1 Query: 474 SRVRWNMSMCSGIGTSRRPKAASNDDFP 391 SR + +M G+GT ++P+ A +D FP Sbjct: 200 SRSAVSGNMNPGVGTLQQPQRAGSDTFP 227 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,817,936 Number of Sequences: 5004 Number of extensions: 54511 Number of successful extensions: 286 Number of sequences better than 10.0: 20 Number of HSP's better than 10.0 without gapping: 161 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 285 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 357280532 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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