Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= brP-0310
(750 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBPB2B2.12c |||UDP-glucose 4-epimerase|Schizosaccharomyces pomb... 163 2e-41
SPBC365.14c |||UDP-glucose 4-epimerase |Schizosaccharomyces pomb... 163 3e-41
SPBC2A9.02 |||NAD dependent epimerase/dehydratase family protein... 40 3e-04
SPAC513.07 |||flavonol reductase/cinnamoyl-CoA reductase family|... 33 0.057
SPAC3G9.02 |oar2||3-oxoacyl-[acyl-carrier-protein] reductase Oar... 30 0.31
SPBC16G5.15c |fkh2||fork head transcription factor Fkh2 |Schizos... 27 2.2
SPBC1773.04 |||flavonol reductase/cinnamoyl-CoA reductase family... 27 2.2
SPBC3F6.02c |||3 beta-hydroxysteroid dehydrogenase/delta 5-->4-i... 27 2.9
SPBC16H5.14c ||SPBC21H7.08|short chain dehydrogenase DHRS family... 27 2.9
SPCC1840.09 |||NAD dependent epimerase/dehydratase family protei... 27 3.8
SPAC186.08c |||L-lactate dehydrogenase |Schizosaccharomyces pomb... 26 6.6
>SPBPB2B2.12c |||UDP-glucose 4-epimerase|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 713
Score = 163 bits (397), Expect = 2e-41
Identities = 91/193 (47%), Positives = 112/193 (58%), Gaps = 4/193 (2%)
Frame = +1
Query: 121 ILVTGGAGYIGSHCVVTLLEAGHEVIAIDNFTNSVEDEDGSPALQRAEKITGKKITFYKA 300
ILVTGGAGYIGSH V+ L+ G++VI +DN NS D A+ R E I K I F+K
Sbjct: 8 ILVTGGAGYIGSHTVIELINHGYKVIIVDNLCNSCYD-----AVARVEFIVRKSIKFFKL 62
Query: 301 DLLDKPQINAIFDKHPVDCVIHFAALKAXGESMQQPXXXXXXXXXXXXXXXEIMRSHNCY 480
DL DK + IFD + VIHFAALKA GESM+ P +MR H
Sbjct: 63 DLRDKEGLAQIFDTFKIKGVIHFAALKAVGESMKLPLEYYDNNICGTITLLNVMREHRVK 122
Query: 481 QMVFSSSCTVYGEPEH----LPITETHXTGSITNVYGRTKYFIEEMLKDLSAADDKWNII 648
+VFSSS TVYG+ +PI E+ TN YG+TKY IE ++KDL +D+ W
Sbjct: 123 TVVFSSSATVYGDATRFDNMIPIPES-CPNDPTNPYGKTKYAIENIIKDLHTSDNTWRGA 181
Query: 649 SLRYFNPVGAHTS 687
LRYFNP+GAH S
Sbjct: 182 ILRYFNPIGAHPS 194
Score = 31.9 bits (69), Expect = 0.10
Identities = 13/20 (65%), Positives = 15/20 (75%)
Frame = +2
Query: 689 GLIGEDPTKXXTNLMPFLAQ 748
GL+GEDP NL+PFLAQ
Sbjct: 195 GLLGEDPLGIPNNLLPFLAQ 214
>SPBC365.14c |||UDP-glucose 4-epimerase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 355
Score = 163 bits (395), Expect = 3e-41
Identities = 87/193 (45%), Positives = 115/193 (59%), Gaps = 4/193 (2%)
Frame = +1
Query: 121 ILVTGGAGYIGSHCVVTLLEAGHEVIAIDNFTNSVEDEDGSPALQRAEKITGKKITFYKA 300
+LVTGGAGYIGSH V LLE G++V+ +DN NS + A+ R EK+TGKK+ F++
Sbjct: 9 VLVTGGAGYIGSHTCVVLLEKGYDVVIVDNLCNSRVE-----AVHRIEKLTGKKVIFHQV 63
Query: 301 DLLDKPQINAIFDKHPVDCVIHFAALKAXGESMQQPXXXXXXXXXXXXXXXEIMRSHNCY 480
DLLD+P ++ +F + VIHFA LKA GES+Q P E M+ +N
Sbjct: 64 DLLDEPALDKVFANQNISAVIHFAGLKAVGESVQVPLSYYKNNISGTINLIECMKKYNVR 123
Query: 481 QMVFSSSCTVYGEPEH----LPITETHXTGSITNVYGRTKYFIEEMLKDLSAADDKWNII 648
VFSSS TVYG+P +PI E+ T+ YGRTK FIE +++D + + N
Sbjct: 124 DFVFSSSATVYGDPTRPGGTIPIPESCPREG-TSPYGRTKLFIENIIEDETKVNKSLNAA 182
Query: 649 SLRYFNPVGAHTS 687
LRYFNP GAH S
Sbjct: 183 LLRYFNPGGAHPS 195
Score = 27.1 bits (57), Expect = 2.9
Identities = 10/20 (50%), Positives = 14/20 (70%)
Frame = +2
Query: 689 GLIGEDPTKXXTNLMPFLAQ 748
G +GEDP NL+P++AQ
Sbjct: 196 GELGEDPLGIPNNLLPYIAQ 215
>SPBC2A9.02 |||NAD dependent epimerase/dehydratase family
protein|Schizosaccharomyces pombe|chr 2|||Manual
Length = 295
Score = 40.3 bits (90), Expect = 3e-04
Identities = 18/28 (64%), Positives = 21/28 (75%)
Frame = +1
Query: 121 ILVTGGAGYIGSHCVVTLLEAGHEVIAI 204
I VTG AG+IGS V LLEAGHEV+ +
Sbjct: 3 IFVTGAAGFIGSEIVRQLLEAGHEVVGL 30
>SPAC513.07 |||flavonol reductase/cinnamoyl-CoA reductase
family|Schizosaccharomyces pombe|chr 1|||Manual
Length = 336
Score = 32.7 bits (71), Expect = 0.057
Identities = 14/27 (51%), Positives = 19/27 (70%)
Frame = +1
Query: 115 KNILVTGGAGYIGSHCVVTLLEAGHEV 195
K +LVTG G+IG+H LL+AG+ V
Sbjct: 4 KLVLVTGVTGFIGAHVAEQLLQAGYRV 30
>SPAC3G9.02 |oar2||3-oxoacyl-[acyl-carrier-protein] reductase Oar2
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 236
Score = 30.3 bits (65), Expect = 0.31
Identities = 20/89 (22%), Positives = 40/89 (44%), Gaps = 1/89 (1%)
Frame = +1
Query: 115 KNILVTGGAGYIGSHCVVTLLEAGHEVIAIDNFTNSVEDEDGSPALQRAEKITGKKITFY 294
+ +L+TGG+ +G + GH+ + +++ LQ G++ T
Sbjct: 2 RKVLITGGSSGLGKRIAQIWSQKGHQCHIVGRNEFHLKE-----TLQSLSVAKGQQHTLT 56
Query: 295 KADL-LDKPQINAIFDKHPVDCVIHFAAL 378
AD+ D + +IF+ +D V+H A +
Sbjct: 57 IADVQSDMKNLKSIFESVEIDTVVHAAGV 85
>SPBC16G5.15c |fkh2||fork head transcription factor Fkh2
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 642
Score = 27.5 bits (58), Expect = 2.2
Identities = 12/36 (33%), Positives = 19/36 (52%)
Frame = +1
Query: 187 HEVIAIDNFTNSVEDEDGSPALQRAEKITGKKITFY 294
HE+ +D+++NS E S +Q K G T+Y
Sbjct: 55 HEMRVVDDYSNSKNAERHSGEIQAYAKFAGSTWTYY 90
>SPBC1773.04 |||flavonol reductase/cinnamoyl-CoA reductase
family|Schizosaccharomyces pombe|chr 2|||Manual
Length = 336
Score = 27.5 bits (58), Expect = 2.2
Identities = 11/36 (30%), Positives = 18/36 (50%)
Frame = +1
Query: 121 ILVTGGAGYIGSHCVVTLLEAGHEVIAIDNFTNSVE 228
+L+TG G++ SH LL G+ V F ++
Sbjct: 5 VLITGITGFVASHSAEALLSQGYRVRGTYRFQEKLD 40
>SPBC3F6.02c |||3 beta-hydroxysteroid dehydrogenase/delta
5-->4-isomerase |Schizosaccharomyces pombe|chr
2|||Manual
Length = 340
Score = 27.1 bits (57), Expect = 2.9
Identities = 22/87 (25%), Positives = 39/87 (44%), Gaps = 1/87 (1%)
Frame = +1
Query: 118 NILVTGGAGYIGSHCVVTLLEAGH-EVIAIDNFTNSVEDEDGSPALQRAEKITGKKITFY 294
N ++ G+G++G H + L E + + A D F N + ++ G+ T Y
Sbjct: 4 NSVLVIGSGFLGGHIIRQLCERENLRIAAFDLFENE----------KLLHELHGQ-FTMY 52
Query: 295 KADLLDKPQINAIFDKHPVDCVIHFAA 375
DL + I +F++ VIH A+
Sbjct: 53 TGDLTKQGDIERVFEEFHPRVVIHTAS 79
>SPBC16H5.14c ||SPBC21H7.08|short chain dehydrogenase DHRS family
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 286
Score = 27.1 bits (57), Expect = 2.9
Identities = 10/30 (33%), Positives = 19/30 (63%)
Frame = +1
Query: 121 ILVTGGAGYIGSHCVVTLLEAGHEVIAIDN 210
I++TGG+G +G + L+ G V ++D+
Sbjct: 38 IVITGGSGILGHAIIQEALDRGFSVASLDS 67
>SPCC1840.09 |||NAD dependent epimerase/dehydratase family
protein|Schizosaccharomyces pombe|chr 3|||Manual
Length = 276
Score = 26.6 bits (56), Expect = 3.8
Identities = 9/28 (32%), Positives = 19/28 (67%)
Frame = +1
Query: 121 ILVTGGAGYIGSHCVVTLLEAGHEVIAI 204
I+V GG+G++G + + G+EV+++
Sbjct: 3 IVVLGGSGFLGHNICKLAIAKGYEVVSV 30
>SPAC186.08c |||L-lactate dehydrogenase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 330
Score = 25.8 bits (54), Expect = 6.6
Identities = 18/44 (40%), Positives = 24/44 (54%), Gaps = 3/44 (6%)
Frame = +1
Query: 112 FKNI-LVTGGAGYIGSHCVVTLLEAG--HEVIAIDNFTNSVEDE 234
FK+I +V GAG +GS TLL +G E++ ID E E
Sbjct: 18 FKSIKIVIVGAGNVGSTTAFTLLLSGLAAEIVIIDLNKKKAEGE 61
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,773,705
Number of Sequences: 5004
Number of extensions: 52428
Number of successful extensions: 160
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 145
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 155
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 357280532
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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