Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= brP-0306
(750 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g57490.1 68418.m07184 porin, putative similar to 36kDA porin ... 65 4e-11
At3g01280.1 68416.m00035 porin, putative similar to SP|P42055 34... 64 7e-11
At5g67500.1 68418.m08512 porin, putative similar to SP|P42055 34... 53 2e-07
At5g37610.1 68418.m04530 hypothetical protein 37 0.012
At3g49920.1 68416.m05458 porin, putative similar to SP|P42055 34... 32 0.47
At2g36340.1 68415.m04460 DNA-binding storekeeper protein-related... 29 2.5
At3g13610.1 68416.m01713 oxidoreductase, 2OG-Fe(II) oxygenase fa... 29 3.3
At3g19840.1 68416.m02513 FF domain-containing protein / WW domai... 28 7.6
>At5g57490.1 68418.m07184 porin, putative similar to 36kDA porin II
[Solanum tuberosum] GI:515360; contains Pfam profile
PF01459: Eukaryotic porin
Length = 274
Score = 65.3 bits (152), Expect = 4e-11
Identities = 53/222 (23%), Positives = 102/222 (45%), Gaps = 1/222 (0%)
Frame = +2
Query: 77 APPYYADLGKKANDVFSKGYHFGVFKLDLKTKSESGVEFTSGITSNQESGKVFGSLSSKF 256
+P +AD+GKKA D+ +K Y F K L S +G EF + T ++ FG +S+ +
Sbjct: 4 SPAPFADIGKKAKDLLNKDYIFD-HKFTLTMLSATGTEFVA--TGLKKDDFFFGDISTLY 60
Query: 257 AVKDYGLTFTEKWNTDNTLATDITIQDKIAAGLKVTLEGTFAPQTGTKTGKLKTSFTNDT 436
K K ++ ++++T +T+++ + + K + K+GKL + +
Sbjct: 61 --KGQNTIVDLKIDSHSSVSTKVTLKNLLPSA-KAVISFKIPDH---KSGKLDVQYVHPH 114
Query: 437 VAVNTNLDLDLAGPVVDVAAVLNYQGWLAGVHTQFDTQKAKFSKNNFALGYQS-GDFALH 613
+N+++ L+ P++D++A + Q G FDT + +K N +G+ + G A
Sbjct: 115 ATLNSSIGLN-PTPLLDLSATIGSQNVCLGGEVSFDTASSSLTKYNAGIGFNNQGVSAAL 173
Query: 614 TNVDNGKDFGGSIYQKVSDKLDCGVSMKWTAGSADTLFGVGS 739
D G+ + V+ G + + + F VGS
Sbjct: 174 ILEDKGESLRATYVHTVNPTTSFGAELIRRFSNYNNSFTVGS 215
>At3g01280.1 68416.m00035 porin, putative similar to SP|P42055 34
kDa outer mitochondrial membrane protein porin
(Voltage-dependent anion-selective channel protein)
(VDAC) {Solanum tuberosum}; contains Pfam profile
PF01459: Eukaryotic porin
Length = 276
Score = 64.5 bits (150), Expect = 7e-11
Identities = 57/224 (25%), Positives = 95/224 (42%), Gaps = 3/224 (1%)
Frame = +2
Query: 80 PPYYADLGKKANDVFSKGYHFGVFKLDLKTKSESGVEFTSGITSNQESGKVFGSLSSKFA 259
P Y ++GKKA D+ K H K + T S +GV TS T ++ + G ++ F
Sbjct: 5 PGLYTEIGKKARDLLYKD-HNSDQKFSITTFSPAGVAITS--TGTKKGDLLLGDVA--FQ 59
Query: 260 VKDYGLTFTEKWNTDNTLATDITIQDKIAAGLKVTLEGTFAPQTGTKTGKLKTSFTNDTV 439
+ +T K TD+T T+ D+ A GL+ Q +GK++ + ++
Sbjct: 60 SRRKNITTDLKVCTDSTFLITATV-DEAAPGLRSIFSFKVPDQN---SGKVELQYLHEYA 115
Query: 440 AVNTNLDLDLAGPVVDVAAVLNYQGWLAGVHTQFDTQKAKFSKNNFALGYQSGDFALHTN 619
++T++ L P V+ + V+ G FDT+ F+K N L + D
Sbjct: 116 GISTSMGLT-QNPTVNFSGVIGSNVLAVGTDVSFDTKSGNFTKINAGLSFTKEDLIASLT 174
Query: 620 V-DNGKDFGGSIYQKVSDKLD--CGVSMKWTAGSADTLFGVGSE 742
V D G S Y V+ + G + S D+ VG++
Sbjct: 175 VNDKGDLLNASYYHIVNPLFNTAVGAEVSHKLSSKDSTITVGTQ 218
>At5g67500.1 68418.m08512 porin, putative similar to SP|P42055 34
kDa outer mitochondrial membrane protein porin
(Voltage-dependent anion-selective channel protein)
(VDAC) {Solanum tuberosum}; contains Pfam profile
PF01459: Eukaryotic porin
Length = 276
Score = 52.8 bits (121), Expect = 2e-07
Identities = 45/177 (25%), Positives = 79/177 (44%)
Frame = +2
Query: 80 PPYYADLGKKANDVFSKGYHFGVFKLDLKTKSESGVEFTSGITSNQESGKVFGSLSSKFA 259
P + D+GKKA D+ ++ Y+ K + T S SGV TS T+ ++ G +++++
Sbjct: 5 PGLFTDIGKKAKDLLTRDYNSDQ-KFSISTYSASGVALTS--TALKKGGVHAADVATQYK 61
Query: 260 VKDYGLTFTEKWNTDNTLATDITIQDKIAAGLKVTLEGTFAPQTGTKTGKLKTSFTNDTV 439
K+ F K +TD+++ T +T+ + I K + P + KL+ + +D
Sbjct: 62 YKN--ALFDVKIDTDSSVLTTVTLTE-ILPSTK-AIASFKVPD--YNSAKLEVQYFHDHA 115
Query: 440 AVNTNLDLDLAGPVVDVAAVLNYQGWLAGVHTQFDTQKAKFSKNNFALGYQSGDFAL 610
V L P++D+ A L G +DT F+K N + D L
Sbjct: 116 TVTAAAALK-QNPLIDITATLGSPVISFGAEAGYDTTSKTFTKYNAGISVTKPDACL 171
>At5g37610.1 68418.m04530 hypothetical protein
Length = 163
Score = 37.1 bits (82), Expect = 0.012
Identities = 22/90 (24%), Positives = 33/90 (36%), Gaps = 1/90 (1%)
Frame = +2
Query: 470 AGPVVDVAAVLNYQGWLAGVHTQFDTQKAKFSKNNFALGYQSGDFALHTNVDNGKD-FGG 646
A P++ +AV W G D +K N L + D ++D F
Sbjct: 16 ANPILSFSAVFGNDFWRFGFELTLDLVNRIITKANTVLSLITEDTTTTFSIDKKASLFTA 75
Query: 647 SIYQKVSDKLDCGVSMKWTAGSADTLFGVG 736
S YQ++ K CG K+ F +G
Sbjct: 76 SYYQRLHSKTVCGAEAKYILSDKSNSFSIG 105
>At3g49920.1 68416.m05458 porin, putative similar to SP|P42055 34
kDa outer mitochondrial membrane protein porin
(Voltage-dependent anion-selective channel protein)
(VDAC) {Solanum tuberosum}; contains Pfam profile
PF01459: Eukaryotic porin
Length = 226
Score = 31.9 bits (69), Expect = 0.47
Identities = 17/45 (37%), Positives = 23/45 (51%)
Frame = +2
Query: 80 PPYYADLGKKANDVFSKGYHFGVFKLDLKTKSESGVEFTSGITSN 214
P +AD+GK A D+ ++ Y K + T S SGV TS N
Sbjct: 5 PGLFADIGKYAKDLLTRDYSTDQ-KFSISTNSVSGVALTSTALKN 48
>At2g36340.1 68415.m04460 DNA-binding storekeeper protein-related
contains similarity to storekeeper protein [Solanum
tuberosum] gi|14268476|emb|CAC39398; contains PF04504:
Protein of unknown function, DUF573
Length = 414
Score = 29.5 bits (63), Expect = 2.5
Identities = 26/94 (27%), Positives = 43/94 (45%), Gaps = 3/94 (3%)
Frame = +2
Query: 296 NTDNTLATDITIQDKIAAGLKVTLEGTFAPQTGTKTGKLKTSFTNDTVAVNTNLDLDLAG 475
N+D+TL + ++ + + L T T T+T L S + + + N +L + G
Sbjct: 14 NSDDTLTRNREVEVEAMLSRRKQLRTTTTRTTTTRTTPLSLSSSASKMNWSKNDELVILG 73
Query: 476 PVVDV--AAVLNYQG-WLAGVHTQFDTQKAKFSK 568
+VD L+Y+ W A D +AKFSK
Sbjct: 74 GIVDYENETKLSYRSDWDALYRYIKDCVEAKFSK 107
>At3g13610.1 68416.m01713 oxidoreductase, 2OG-Fe(II) oxygenase
family protein similar to desacetoxyvindoline
4-hydroxylase [Catharanthus roseus][GI:1916643],
flavonol synthase 1 [SP|Q96330]; contains PF03171
2OG-Fe(II) oxygenase superfamily domain
Length = 361
Score = 29.1 bits (62), Expect = 3.3
Identities = 18/59 (30%), Positives = 27/59 (45%), Gaps = 1/59 (1%)
Frame = -1
Query: 351 PAAILSWIVMSVANVLSVFHFSVKVKP*SFTANLEERLPKTFPLSWLEVIPLVN-STPD 178
PA + ++V V + +K P + LEERL F E IP+++ S PD
Sbjct: 13 PAEVTDFVVYKGNGVKGLSETGIKALPEQYIQPLEERLINKFVNETDEAIPVIDMSNPD 71
>At3g19840.1 68416.m02513 FF domain-containing protein / WW
domain-containing protein weak similarity to
transcription factor CA150b [Mus musculus] GI:6329166;
contains Pfam profiles PF01846: FF domain, PF00397: WW
domain
Length = 743
Score = 27.9 bits (59), Expect = 7.6
Identities = 20/56 (35%), Positives = 30/56 (53%), Gaps = 2/56 (3%)
Frame = +2
Query: 68 TDMAPPYYADLGKKANDVFSKGYHFGVFKLDLKTKS--ESGVEFTSGITSNQESGK 229
T ++ P ++ G+ A + K +FG LDL K +SG+ +S ITS SGK
Sbjct: 268 TSLSAPAISNGGRDAASL--KTTNFGSSALDLVKKKLHDSGMPVSSTITSEANSGK 321
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 15,985,988
Number of Sequences: 28952
Number of extensions: 335011
Number of successful extensions: 882
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 846
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 880
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1663169840
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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