Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= brP-0304
(750 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC6G10.08 |idp1||isocitrate dehydrogenase Idp1|Schizosaccharom... 367 e-103
SPAC24H6.05 |cdc25|sal2|serine/threonine protein phosphatase Cdc... 28 1.2
SPAC688.04c |gst3||glutathione S-transferase |Schizosaccharomyce... 27 2.2
SPAC11D3.13 |||ThiJ domain protein|Schizosaccharomyces pombe|chr... 27 2.2
SPAC144.17c |||6-phosphofructo-2-kinase|Schizosaccharomyces pomb... 27 2.2
SPAC15A10.08 |ain1||alpha-actinin|Schizosaccharomyces pombe|chr ... 27 2.9
SPAC3F10.04 |gsa1|gsh2|glutathione synthetase large subunit Gsa1... 27 2.9
SPBC6B1.05c |||ubiquitin-like conjugating enzyme|Schizosaccharom... 27 3.8
SPBC83.11 |||triose phosphate transporter|Schizosaccharomyces po... 25 8.7
SPAC1F5.07c |hem14||protoporphyrinogen oxidase|Schizosaccharomyc... 25 8.7
>SPAC6G10.08 |idp1||isocitrate dehydrogenase
Idp1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 418
Score = 367 bits (904), Expect = e-103
Identities = 165/248 (66%), Positives = 203/248 (81%), Gaps = 2/248 (0%)
Frame = +2
Query: 11 MDGDEMTRIMWAKIKERLIFPYVKVDCLYFDLGLPHRDATDDQVTIDSAHAILKHNVGIK 190
MDGDEMTR++W I+E+L+ PY+ + Y+DLG+ RD T+DQ+T+D+A AILK++VGIK
Sbjct: 23 MDGDEMTRVIWKIIREKLVLPYMDIKLDYYDLGIEARDKTNDQITVDAAKAILKNDVGIK 82
Query: 191 CATITPDEQRVEEFKLKKMWLSPNGTIRNILGGTVFREPILCQSIPRVVPGWTKPIVIGR 370
CATITPDE RV+E+ LKKMW SPNGTIRNIL GTVFREPIL ++IP+ +PGWT PI IGR
Sbjct: 83 CATITPDEARVKEYNLKKMWKSPNGTIRNILNGTVFREPILIKNIPKYIPGWTNPICIGR 142
Query: 371 HAHGDQYKAQDFVVPKPGKVELVYTTRDGTTERRV--LYDFKTPGVAMGMYNTDESIRSF 544
HA GDQYK+ D V PGK+EL +T + + + +Y+F GVAM MYNTD+SIR F
Sbjct: 143 HAFGDQYKSTDLVASGPGKLELSFTPKGNPSAKETYNVYEFNGSGVAMSMYNTDDSIRGF 202
Query: 545 AHSSFQVALQKKWPLYLSTKNTILKRYDGRFKDIFEEVFQSDYKTKFDEAKIWYEHRLID 724
AHSSFQ+ALQKK PLYLSTKNTILK+YDGRFKD F+EV++SDYK KF+E +WY+HRLID
Sbjct: 203 AHSSFQMALQKKMPLYLSTKNTILKKYDGRFKDTFQEVYESDYKQKFEELGLWYQHRLID 262
Query: 725 DMVAQAIK 748
DMVAQAIK
Sbjct: 263 DMVAQAIK 270
>SPAC24H6.05 |cdc25|sal2|serine/threonine protein phosphatase
Cdc25|Schizosaccharomyces pombe|chr 1|||Manual
Length = 596
Score = 28.3 bits (60), Expect = 1.2
Identities = 11/30 (36%), Positives = 20/30 (66%)
Frame = +2
Query: 599 TKNTILKRYDGRFKDIFEEVFQSDYKTKFD 688
T+ T+L DG+FKDIF++ D + +++
Sbjct: 414 TQETLLGLLDGKFKDIFDKCIIIDCRFEYE 443
>SPAC688.04c |gst3||glutathione S-transferase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 242
Score = 27.5 bits (58), Expect = 2.2
Identities = 14/41 (34%), Positives = 22/41 (53%)
Frame = +2
Query: 131 DDQVTIDSAHAILKHNVGIKCATITPDEQRVEEFKLKKMWL 253
DD VT + AIL+H V + P E+ V E + ++W+
Sbjct: 56 DDGVTYIESAAILEHLVRKYGPSFKPSEEDVAELEKYELWM 96
>SPAC11D3.13 |||ThiJ domain protein|Schizosaccharomyces pombe|chr
1|||Manual
Length = 222
Score = 27.5 bits (58), Expect = 2.2
Identities = 10/22 (45%), Positives = 16/22 (72%)
Frame = +1
Query: 574 EKMASIPVYEEYHFETLRWSFQ 639
++M +P +E+YHF+TL FQ
Sbjct: 156 QQMNLMPTFEKYHFKTLNKLFQ 177
>SPAC144.17c |||6-phosphofructo-2-kinase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 432
Score = 27.5 bits (58), Expect = 2.2
Identities = 24/96 (25%), Positives = 45/96 (46%), Gaps = 6/96 (6%)
Frame = +2
Query: 404 FVVPKPGKVELVYTTRDGTTERRVLYD-FKTPGVAM----GMYNTDESIRSFAHSSFQVA 568
F++ + G+V + T RR+LYD FK G + + N ++ I + + V+
Sbjct: 130 FLLKEKGQVAIYDATNGTRRTRRILYDRFKNCGFKILFIESLCNKEDVINANIQEAIHVS 189
Query: 569 LQ-KKWPLYLSTKNTILKRYDGRFKDIFEEVFQSDY 673
+ + W L ++ K +R D K +E + + DY
Sbjct: 190 EEFRNWDLEMAEKE-YCRRID-ILKCHYETIDEKDY 223
>SPAC15A10.08 |ain1||alpha-actinin|Schizosaccharomyces pombe|chr
1|||Manual
Length = 621
Score = 27.1 bits (57), Expect = 2.9
Identities = 16/34 (47%), Positives = 19/34 (55%)
Frame = -1
Query: 570 KAT*KLEWAKDRIDSSVLYMPIATPGVLKSYNTR 469
KAT K EW K++ID L I T LK+Y R
Sbjct: 300 KATEKREWVKEKIDLESLLGTIQTN--LKTYQLR 331
>SPAC3F10.04 |gsa1|gsh2|glutathione synthetase large subunit
Gsa1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 498
Score = 27.1 bits (57), Expect = 2.9
Identities = 12/44 (27%), Positives = 22/44 (50%)
Frame = +2
Query: 569 LQKKWPLYLSTKNTILKRYDGRFKDIFEEVFQSDYKTKFDEAKI 700
+ K W LY + + + +F+ + VF+SDY D++ I
Sbjct: 97 MNKLWNLYQKHREAVAHLKENQFQPLSLGVFRSDYMVHQDDSFI 140
>SPBC6B1.05c |||ubiquitin-like conjugating
enzyme|Schizosaccharomyces pombe|chr 2|||Manual
Length = 649
Score = 26.6 bits (56), Expect = 3.8
Identities = 20/56 (35%), Positives = 26/56 (46%), Gaps = 2/56 (3%)
Frame = +3
Query: 303 NRYCVRVSRGL--CLVGRNLL**AVMRTATNTRRKILLYRNPERLNLFTLHEMVRQ 464
N C+ + G C V RNLL V R T + Y NP R +LFT + R+
Sbjct: 335 NSKCLLLGAGTLGCGVARNLLSWGV-RHVTFVDYSTVSYSNPVRQSLFTFEDCKRK 389
>SPBC83.11 |||triose phosphate transporter|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 434
Score = 25.4 bits (53), Expect = 8.7
Identities = 15/41 (36%), Positives = 23/41 (56%)
Frame = -1
Query: 285 PKMLRIVPFGLSHIFFNLNSSTLCSSGVIVAHLMPTLCFRM 163
P L ++ GLSH F N+ + TL S VA+ + +L R+
Sbjct: 223 PVFLNLIYNGLSHFFQNILAFTLLSIISPVAYSIASLIKRI 263
>SPAC1F5.07c |hem14||protoporphyrinogen oxidase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 490
Score = 25.4 bits (53), Expect = 8.7
Identities = 12/21 (57%), Positives = 15/21 (71%)
Frame = -1
Query: 75 YGNISLSLILAHMMRVISSPS 13
YGNI+L LI A + R I SP+
Sbjct: 201 YGNITLGLIRALLAREILSPA 221
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,301,487
Number of Sequences: 5004
Number of extensions: 72295
Number of successful extensions: 206
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 191
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 205
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 357280532
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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