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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= brP-0304
         (750 letters)

Database: nematostella 
           59,808 sequences; 16,821,457 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SB_17538| Best HMM Match : Iso_dh (HMM E-Value=0)                     340   7e-94
SB_26442| Best HMM Match : Iso_dh (HMM E-Value=3.6e-37)               272   2e-73
SB_35142| Best HMM Match : Peptidase_M1 (HMM E-Value=2e-13)            33   0.33 
SB_31870| Best HMM Match : No HMM Matches (HMM E-Value=.)              31   0.76 
SB_52148| Best HMM Match : EGF (HMM E-Value=0)                         31   1.3  
SB_26728| Best HMM Match : Disintegrin (HMM E-Value=1.9e-23)           29   3.0  
SB_36984| Best HMM Match : No HMM Matches (HMM E-Value=.)              29   4.0  
SB_29733| Best HMM Match : No HMM Matches (HMM E-Value=.)              29   4.0  
SB_42530| Best HMM Match : ELFV_dehydrog_N (HMM E-Value=0)             28   9.3  
SB_8123| Best HMM Match : Tfb2 (HMM E-Value=0.00019)                   28   9.3  
SB_6680| Best HMM Match : Astacin (HMM E-Value=0)                      28   9.3  

>SB_17538| Best HMM Match : Iso_dh (HMM E-Value=0)
          Length = 644

 Score =  340 bits (836), Expect = 7e-94
 Identities = 153/231 (66%), Positives = 189/231 (81%), Gaps = 1/231 (0%)
 Frame = +2

Query: 59  RLIFPYVKVDCLYFDLGLPHRDATDDQVTIDSAHAILKHNVGIKCATITPDEQRVEEFKL 238
           +LI PY+ +D  YFDLGL HRDAT+D+VT+++A AI KH+VGIKCATITPDE RVEEFKL
Sbjct: 19  QLISPYLDLDIKYFDLGLEHRDATNDKVTVEAAEAIRKHHVGIKCATITPDEARVEEFKL 78

Query: 239 KKMWLSPNGTIRNILGGTVFREPILCQSIPRVVPGWTKPIVIGRHAHGDQYKAQDFVVPK 418
           KKMW SPNGTIRNILGGTVFR PI+C+++PR+VPGW KPIVIGRHA GDQY+A+DF V  
Sbjct: 79  KKMWKSPNGTIRNILGGTVFRAPIICKTVPRLVPGWEKPIVIGRHAFGDQYRARDFAVNG 138

Query: 419 PGKVELVYTTRDGTTERRVLYDFK-TPGVAMGMYNTDESIRSFAHSSFQVALQKKWPLYL 595
           PG  E+ +T   G      +++F  T GV MGMYNTDE+IR FAHS  Q A+ K+ PLY+
Sbjct: 139 PGSFEISFTPESGGKLTTEVFEFTGTGGVMMGMYNTDEAIRDFAHSCMQYAIHKQVPLYM 198

Query: 596 STKNTILKRYDGRFKDIFEEVFQSDYKTKFDEAKIWYEHRLIDDMVAQAIK 748
           STKNTILK+YDGRFKDIF+++++ +Y+++F E  IWYEHRLIDDMVA A+K
Sbjct: 199 STKNTILKKYDGRFKDIFQDIYEREYESQFKELGIWYEHRLIDDMVAYALK 249


>SB_26442| Best HMM Match : Iso_dh (HMM E-Value=3.6e-37)
          Length = 296

 Score =  272 bits (667), Expect = 2e-73
 Identities = 138/219 (63%), Positives = 165/219 (75%), Gaps = 2/219 (0%)
 Frame = +2

Query: 98  FDLGLPHRDATDDQVTIDSAHAILKHNVGIKCATITPDEQRVEEFKLKKMWLSPNGTIRN 277
           +DL + +RDAT+DQVTID+A AI + +VGIKCATITPDE+RVEEFKLKKMW SPNGTIRN
Sbjct: 6   YDLSIENRDATNDQVTIDAAAAIKQCHVGIKCATITPDEKRVEEFKLKKMWKSPNGTIRN 65

Query: 278 ILGGTVFREPILCQSIPRVVPGWTKPIVIGRHAHGDQYKAQDFVVPKPGKVELVYTTRDG 457
           ILGGTVFRE I+C++IPR+V  WTKPI+IGRHAH DQYKA DFVVP PGKVE+VY+  DG
Sbjct: 66  ILGGTVFREAIICKNIPRLVSPWTKPIIIGRHAHADQYKATDFVVPGPGKVEIVYSPADG 125

Query: 458 TTE-RRVLYDFKT-PGVAMGMYNTDESIRSFAHSSFQVALQKKWPLYLSTKNTILKRYDG 631
               +  + +FK   GV MGM+NTD SIR+FAHSSFQ AL KK+PLY+            
Sbjct: 126 GEPIKYTVNEFKDGGGVTMGMFNTDVSIRAFAHSSFQYALDKKYPLYM------------ 173

Query: 632 RFKDIFEEVFQSDYKTKFDEAKIWYEHRLIDDMVAQAIK 748
                       +YK+KF+E+ IWYEHRLIDDMVA A+K
Sbjct: 174 -----------REYKSKFEESNIWYEHRLIDDMVAFALK 201


>SB_35142| Best HMM Match : Peptidase_M1 (HMM E-Value=2e-13)
          Length = 302

 Score = 32.7 bits (71), Expect = 0.33
 Identities = 15/55 (27%), Positives = 26/55 (47%)
 Frame = +2

Query: 560 QVALQKKWPLYLSTKNTILKRYDGRFKDIFEEVFQSDYKTKFDEAKIWYEHRLID 724
           Q+ L+ K  L    K TI   + G   +     ++S YKT+  E ++W +   +D
Sbjct: 167 QLLLEPKQGLVKGQKFTIKLEFSGELSNKMAGFYKSSYKTRNGEVRVWAQKEALD 221


>SB_31870| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 1378

 Score = 31.5 bits (68), Expect = 0.76
 Identities = 17/63 (26%), Positives = 27/63 (42%)
 Frame = +2

Query: 107 GLPHRDATDDQVTIDSAHAILKHNVGIKCATITPDEQRVEEFKLKKMWLSPNGTIRNILG 286
           G+ HRD   D + +     +   + G  CATITP++ +         W++P    R   G
Sbjct: 336 GVIHRDIKSDNILLGMDGQVKLTDFGF-CATITPEQNKRSTMVGTPYWMAPEVVTRKQYG 394

Query: 287 GTV 295
             V
Sbjct: 395 PRV 397


>SB_52148| Best HMM Match : EGF (HMM E-Value=0)
          Length = 1055

 Score = 30.7 bits (66), Expect = 1.3
 Identities = 18/58 (31%), Positives = 26/58 (44%)
 Frame = +2

Query: 389 YKAQDFVVPKPGKVELVYTTRDGTTERRVLYDFKTPGVAMGMYNTDESIRSFAHSSFQ 562
           YKA+D  +PK     L   TR+G  + R  Y  + P    G +N  + I S     F+
Sbjct: 95  YKAEDCSIPKREAPVLYGVTREGLCDLRYYYCKRFPLTGKGFFNNRKLICSVRQILFR 152


>SB_26728| Best HMM Match : Disintegrin (HMM E-Value=1.9e-23)
          Length = 1531

 Score = 29.5 bits (63), Expect = 3.0
 Identities = 16/47 (34%), Positives = 27/47 (57%), Gaps = 1/47 (2%)
 Frame = +2

Query: 17  GDEMTRIMWAKIKE-RLIFPYVKVDCLYFDLGLPHRDATDDQVTIDS 154
           G+E+  ++W+   + RL   ++K DC+   LGL + D  D Q+T  S
Sbjct: 432 GNEVELLLWSGCDDQRLELWFMKQDCV-LPLGLRNGDIPDSQITASS 477


>SB_36984| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 455

 Score = 29.1 bits (62), Expect = 4.0
 Identities = 16/63 (25%), Positives = 30/63 (47%)
 Frame = +2

Query: 557 FQVALQKKWPLYLSTKNTILKRYDGRFKDIFEEVFQSDYKTKFDEAKIWYEHRLIDDMVA 736
           F++  +KKW LYLS K    +     + + + +  +S    K   A +   H    D++A
Sbjct: 68  FELPPEKKWQLYLSKKKEQAEHSSTSYPEFYIDQLKSLNSQKHGRAHV-LAHPTCIDIIA 126

Query: 737 QAI 745
           Q++
Sbjct: 127 QSL 129


>SB_29733| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 360

 Score = 29.1 bits (62), Expect = 4.0
 Identities = 12/33 (36%), Positives = 20/33 (60%)
 Frame = +3

Query: 387 NTRRKILLYRNPERLNLFTLHEMVRQRGVYYMI 485
           N  R+ LL +     N+  L+E ++QRGVY ++
Sbjct: 60  NILREALLLKRAHHPNIVKLYETLKQRGVYCLV 92


>SB_42530| Best HMM Match : ELFV_dehydrog_N (HMM E-Value=0)
          Length = 520

 Score = 27.9 bits (59), Expect = 9.3
 Identities = 18/72 (25%), Positives = 34/72 (47%)
 Frame = +2

Query: 209 DEQRVEEFKLKKMWLSPNGTIRNILGGTVFREPILCQSIPRVVPGWTKPIVIGRHAHGDQ 388
           D + +E++KL+K      GTI N  G  +  E +L      ++P   +  +  ++AH  +
Sbjct: 295 DPKELEDYKLEK------GTITNYPGAKMVEENLLEAECDILIPAANEKQITKKNAHRIK 348

Query: 389 YKAQDFVVPKPG 424
            K +  +   PG
Sbjct: 349 AKEKGTITNYPG 360


>SB_8123| Best HMM Match : Tfb2 (HMM E-Value=0.00019)
          Length = 448

 Score = 27.9 bits (59), Expect = 9.3
 Identities = 16/44 (36%), Positives = 26/44 (59%), Gaps = 1/44 (2%)
 Frame = -1

Query: 351 FVQPGTTLGIL*HNIGSRNTVPP-KMLRIVPFGLSHIFFNLNSS 223
           F  P T L +  +NIGS NT+   ++ R VP G+ H  + +N++
Sbjct: 38  FCHPATCLTVFRYNIGSLNTLKQLRIWREVPSGV-HKRYEMNAT 80


>SB_6680| Best HMM Match : Astacin (HMM E-Value=0)
          Length = 637

 Score = 27.9 bits (59), Expect = 9.3
 Identities = 11/43 (25%), Positives = 23/43 (53%)
 Frame = +2

Query: 224 EEFKLKKMWLSPNGTIRNILGGTVFREPILCQSIPRVVPGWTK 352
           E ++ K++ L P G +  ++G  + R P   + I + + GW +
Sbjct: 76  EPWERKQVQLWPGGVVPYLIGEDLKRRPSFVKKIEQAMEGWER 118


  Database: nematostella
    Posted date:  Oct 22, 2007  1:22 PM
  Number of letters in database: 16,821,457
  Number of sequences in database:  59,808
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 25,331,339
Number of Sequences: 59808
Number of extensions: 559848
Number of successful extensions: 1446
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 1341
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1441
length of database: 16,821,457
effective HSP length: 80
effective length of database: 12,036,817
effective search space used: 2034222073
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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