Bombyx BLAST Result against arabidopsis

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= brP-0304
         (750 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At5g14590.1 68418.m01711 isocitrate dehydrogenase, putative / NA...   376   e-105
At1g65930.1 68414.m07481 isocitrate dehydrogenase, putative / NA...   373   e-104
At1g54340.1 68414.m06195 isocitrate dehydrogenase, putative / NA...   366   e-102
At3g30848.1 68416.m03974 hypothetical protein contains similarit...    30   1.9  
At4g15480.1 68417.m02366 UDP-glucoronosyl/UDP-glucosyl transfera...    28   5.8  
At2g33200.1 68415.m04067 F-box family protein contains F-box dom...    28   5.8  
At2g04280.1 68415.m00420 expressed protein                             28   5.8  
At1g30730.1 68414.m03756 FAD-binding domain-containing protein s...    28   5.8  
At1g53900.1 68414.m06136 eukaryotic translation initiation facto...    28   7.6  
At1g53880.1 68414.m06133 eukaryotic translation initiation facto...    28   7.6  

>At5g14590.1 68418.m01711 isocitrate dehydrogenase, putative / NADP+
           isocitrate dehydrogenase, putative strong similarity to
           isocitrate dehydrogenase (NADP+) [Nicotiana tabacum]
           GI:3021512; contains Pfam domain PF00180: dehydrogenase,
           isocitrate/isopropylmalate family
          Length = 485

 Score =  376 bits (926), Expect = e-105
 Identities = 169/247 (68%), Positives = 205/247 (82%), Gaps = 1/247 (0%)
 Frame = +2

Query: 11  MDGDEMTRIMWAKIKERLIFPYVKVDCLYFDLGLPHRDATDDQVTIDSAHAILKHNVGIK 190
           MDGDEMTR++W+ IKE+LI PY+ +D  YFDLG+ +RDATDD+VT++SA A LK+NV IK
Sbjct: 85  MDGDEMTRVIWSMIKEKLILPYLDLDIKYFDLGILNRDATDDKVTVESAEAALKYNVAIK 144

Query: 191 CATITPDEQRVEEFKLKKMWLSPNGTIRNILGGTVFREPILCQSIPRVVPGWTKPIVIGR 370
           CATITPDE RV+EF LK MW SPNGTIRNIL GTVFREPI+C +IPR+VPGW KPI IGR
Sbjct: 145 CATITPDEGRVKEFGLKSMWRSPNGTIRNILDGTVFREPIMCSNIPRLVPGWEKPICIGR 204

Query: 371 HAHGDQYKAQDFVVPKPGKVELVYTTRDGTTERRV-LYDFKTPGVAMGMYNTDESIRSFA 547
           HA GDQY+A D V+  PGK+++V+   DG     + +YDFK PGVA+ MYN DESIR+FA
Sbjct: 205 HAFGDQYRATDTVIKGPGKLKMVFVPEDGNAPVELDVYDFKGPGVALAMYNVDESIRAFA 264

Query: 548 HSSFQVALQKKWPLYLSTKNTILKRYDGRFKDIFEEVFQSDYKTKFDEAKIWYEHRLIDD 727
            SS  +AL KKWPLYLSTKNTILK+YDGRFKDIF+EV+++++K KF+E  IWYEHRLIDD
Sbjct: 265 ESSMAMALTKKWPLYLSTKNTILKKYDGRFKDIFQEVYEANWKQKFEEHSIWYEHRLIDD 324

Query: 728 MVAQAIK 748
           MVA A+K
Sbjct: 325 MVAYAVK 331


>At1g65930.1 68414.m07481 isocitrate dehydrogenase, putative / NADP+
           isocitrate dehydrogenase, putative strong similarity to
           isocitrate dehydrogenase SP|Q40345 from [Medicago
           sativa]
          Length = 410

 Score =  373 bits (917), Expect = e-104
 Identities = 167/246 (67%), Positives = 202/246 (82%)
 Frame = +2

Query: 11  MDGDEMTRIMWAKIKERLIFPYVKVDCLYFDLGLPHRDATDDQVTIDSAHAILKHNVGIK 190
           MDGDEMTR++W  IK++LI P+V++D  YFDLGLPHRDATDD+VTI+SA A  K+NV IK
Sbjct: 15  MDGDEMTRVIWKSIKDKLITPFVELDIKYFDLGLPHRDATDDKVTIESAEATKKYNVAIK 74

Query: 191 CATITPDEQRVEEFKLKKMWLSPNGTIRNILGGTVFREPILCQSIPRVVPGWTKPIVIGR 370
           CATITPDE RV EF LK+MW SPNGTIRNIL GTVFREPI+C+++P++VPGWTKPI IGR
Sbjct: 75  CATITPDEGRVTEFGLKQMWRSPNGTIRNILNGTVFREPIICKNVPKLVPGWTKPICIGR 134

Query: 371 HAHGDQYKAQDFVVPKPGKVELVYTTRDGTTERRVLYDFKTPGVAMGMYNTDESIRSFAH 550
           HA GDQY+A D V+  PGK+ + +  +DG TE  V       GVAM MYNTDESIR+FA 
Sbjct: 135 HAFGDQYRATDAVIKGPGKLTMTFEGKDGKTETEVFTFTGEGGVAMAMYNTDESIRAFAD 194

Query: 551 SSFQVALQKKWPLYLSTKNTILKRYDGRFKDIFEEVFQSDYKTKFDEAKIWYEHRLIDDM 730
           +S   A +KKWPLYLSTKNTILK+YDGRFKDIF+EV+++ +K+K+D A IWYEHRLIDDM
Sbjct: 195 ASMNTAYEKKWPLYLSTKNTILKKYDGRFKDIFQEVYEASWKSKYDAAGIWYEHRLIDDM 254

Query: 731 VAQAIK 748
           VA A+K
Sbjct: 255 VAYALK 260


>At1g54340.1 68414.m06195 isocitrate dehydrogenase, putative / NADP+
           isocitrate dehydrogenase, putative strong similarity to
           NADP-isocitrate dehydrogenase GI:5764653 from [Citrus
           limon]; Nicotiana tabacum SP|P50218
          Length = 416

 Score =  366 bits (901), Expect = e-102
 Identities = 165/247 (66%), Positives = 206/247 (83%), Gaps = 1/247 (0%)
 Frame = +2

Query: 11  MDGDEMTRIMWAKIKERLIFPYVKVDCLYFDLGLPHRDATDDQVTIDSAHAILKHNVGIK 190
           MDGDEMTR++W  IK++LIFP++++D  YFDLGLP+RD TDD+VTI++A A LK+NV IK
Sbjct: 15  MDGDEMTRVIWKFIKDKLIFPFLELDIKYFDLGLPNRDFTDDKVTIETAEATLKYNVAIK 74

Query: 191 CATITPDEQRVEEFKLKKMWLSPNGTIRNILGGTVFREPILCQSIPRVVPGWTKPIVIGR 370
           CATITPDE RV EF LKKMW SPNGTIRNIL GTVFREPI+C++IPR+VPGWTKPI IGR
Sbjct: 75  CATITPDEARVREFGLKKMWRSPNGTIRNILNGTVFREPIICRNIPRLVPGWTKPICIGR 134

Query: 371 HAHGDQYKAQDFVVPKPGKVELVYTTRDGTTERRV-LYDFKTPGVAMGMYNTDESIRSFA 547
           HA GDQY+A D +V +PGK++LV+     + +    +++F   GVA+ MYNTDESIR+FA
Sbjct: 135 HAFGDQYRATDLIVNEPGKLKLVFEPSGSSQKTEFEVFNFTGGGVALAMYNTDESIRAFA 194

Query: 548 HSSFQVALQKKWPLYLSTKNTILKRYDGRFKDIFEEVFQSDYKTKFDEAKIWYEHRLIDD 727
            SS   A QKKWPLYLSTKNTILK YDGRFKDIF+EV+++++++K++ A IWYEHRLIDD
Sbjct: 195 ESSMYTAYQKKWPLYLSTKNTILKIYDGRFKDIFQEVYEANWRSKYEAAGIWYEHRLIDD 254

Query: 728 MVAQAIK 748
           MVA A+K
Sbjct: 255 MVAYAMK 261


>At3g30848.1 68416.m03974 hypothetical protein contains similarity
           to hypothetical proteins
          Length = 156

 Score = 29.9 bits (64), Expect = 1.9
 Identities = 14/40 (35%), Positives = 20/40 (50%)
 Frame = +2

Query: 596 STKNTILKRYDGRFKDIFEEVFQSDYKTKFDEAKIWYEHR 715
           STK     + D    ++  ++F +DYKTKF     W E R
Sbjct: 49  STKEKTSGQNDNYVVNLAHQIFYTDYKTKFSLEHAWREVR 88


>At4g15480.1 68417.m02366 UDP-glucoronosyl/UDP-glucosyl transferase
           family protein contains Pfam profile: PF00201
           UDP-glucoronosyl and UDP-glucosyl transferase
          Length = 490

 Score = 28.3 bits (60), Expect = 5.8
 Identities = 15/31 (48%), Positives = 18/31 (58%), Gaps = 1/31 (3%)
 Frame = -1

Query: 573 CKAT*K-LEWAKDRIDSSVLYMPIATPGVLK 484
           CK+T K LEW   R  SSV+Y+   T   LK
Sbjct: 271 CKSTDKCLEWLDSRPKSSVVYISFGTVAYLK 301


>At2g33200.1 68415.m04067 F-box family protein contains F-box domain
           Pfam:PF00646
          Length = 376

 Score = 28.3 bits (60), Expect = 5.8
 Identities = 12/39 (30%), Positives = 22/39 (56%)
 Frame = +1

Query: 235 VEENVAKPERNDP*HFGRYSIPRTDIVSEYPEGCAWLDE 351
           +E ++   ER +     ++ I RTDI+S   + C W++E
Sbjct: 119 MESSILGKERLEKEVAWKHFIERTDIISTKKQACLWINE 157


>At2g04280.1 68415.m00420 expressed protein
          Length = 568

 Score = 28.3 bits (60), Expect = 5.8
 Identities = 16/52 (30%), Positives = 27/52 (51%)
 Frame = +2

Query: 551 SSFQVALQKKWPLYLSTKNTILKRYDGRFKDIFEEVFQSDYKTKFDEAKIWY 706
           S+ Q  +++   LY++T    L  ++   KD +   F  DYK  +DE+  WY
Sbjct: 462 STLQDKVEEGRHLYIATNEGELSFFNP-LKDKYATHFLYDYKDLWDESSEWY 512


>At1g30730.1 68414.m03756 FAD-binding domain-containing protein
           similar to SP|P30986 reticuline oxidase precursor
           (Berberine-bridge-forming enzyme) (BBE)
           (Tetrahydroprotoberberine synthase) [Eschscholzia
           californica]; contains PF01565 FAD binding domain
          Length = 526

 Score = 28.3 bits (60), Expect = 5.8
 Identities = 14/29 (48%), Positives = 16/29 (55%)
 Frame = +2

Query: 635 FKDIFEEVFQSDYKTKFDEAKIWYEHRLI 721
           FKD FE +   D KTKFDE   W   + I
Sbjct: 496 FKDNFERLV--DIKTKFDEINFWRNEQSI 522


>At1g53900.1 68414.m06136 eukaryotic translation initiation factor
           2B family protein / eIF-2B family protein similar to
           SP|Q64270 Translation initiation factor eIF-2B alpha
           subunit {Rattus norvegicus}; contains Pfam profiles
           PF04525: Protein of unknown function (DUF567), PF01008:
           Initiation factor 2 subunit family
          Length = 644

 Score = 27.9 bits (59), Expect = 7.6
 Identities = 17/48 (35%), Positives = 24/48 (50%)
 Frame = +2

Query: 401 DFVVPKPGKVELVYTTRDGTTERRVLYDFKTPGVAMGMYNTDESIRSF 544
           DF VP P KVE+  + RD T  + +   F   GV      +DE I+ +
Sbjct: 596 DFSVPVPPKVEVERSARDYTPPQYLTLLFTDLGVLTPSVVSDELIQLY 643


>At1g53880.1 68414.m06133 eukaryotic translation initiation factor
           2B family protein / eIF-2B family protein similar to
           SP|Q64270 Translation initiation factor eIF-2B alpha
           subunit {Rattus norvegicus}; contains Pfam profiles
           PF04525: Protein of unknown function (DUF567), PF01008:
           Initiation factor 2 subunit family
          Length = 637

 Score = 27.9 bits (59), Expect = 7.6
 Identities = 17/48 (35%), Positives = 24/48 (50%)
 Frame = +2

Query: 401 DFVVPKPGKVELVYTTRDGTTERRVLYDFKTPGVAMGMYNTDESIRSF 544
           DF VP P KVE+  + RD T  + +   F   GV      +DE I+ +
Sbjct: 589 DFSVPVPPKVEVERSARDYTPPQYLTLLFTDLGVLTPSVVSDELIQLY 636


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 17,402,791
Number of Sequences: 28952
Number of extensions: 382646
Number of successful extensions: 940
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 917
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 937
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1663169840
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)