Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= brP-0302
(600 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC186.08c |||L-lactate dehydrogenase |Schizosaccharomyces pomb... 134 9e-33
SPBC1539.07c |||glutathione-dependent formaldehyde dehydrogenase... 31 0.17
SPBC1347.04 |tim54||TIM22 inner membrane protein import complex ... 28 1.2
SPAC10F6.09c |psm3|smc3|mitotic cohesin complex subunit Psm3|Sch... 27 2.1
SPAC821.09 |eng1||endo-1,3-beta-glucanase Eng1|Schizosaccharomyc... 26 4.8
SPAPB1A10.07c |||sphingolipid biosynthesis protein|Schizosacchar... 26 4.8
SPCC320.03 |||transcription factor |Schizosaccharomyces pombe|ch... 25 6.4
SPBC16A3.07c |nrm1||negative regulator of MBF|Schizosaccharomyce... 25 6.4
SPAC664.10 |klp2||kinesin-like protein Klp2|Schizosaccharomyces ... 25 6.4
SPAC3H8.05c |||conserved fungal protein|Schizosaccharomyces pomb... 25 8.5
SPCC24B10.20 |||short chain dehydrogenase |Schizosaccharomyces p... 25 8.5
SPBC146.12 |coq6||monooxygenase Coq6|Schizosaccharomyces pombe|c... 25 8.5
SPAC3H1.06c |||membrane transporter |Schizosaccharomyces pombe|c... 25 8.5
>SPAC186.08c |||L-lactate dehydrogenase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 330
Score = 134 bits (324), Expect = 9e-33
Identities = 61/148 (41%), Positives = 93/148 (62%)
Frame = +3
Query: 114 KVTIVGVGQVGMAAAFSMLTQNVTNNIALVDMMADKLKGEMMDLQHGSAFMRNAKIQSST 293
K+ IVG G VG AF++L + I ++D+ K +GE MDL H + ++
Sbjct: 22 KIVIVGAGNVGSTTAFTLLLSGLAAEIVIIDLNKKKAEGEAMDLNHAAPLSHETRVYLG- 80
Query: 294 DYSITAGSKICVVTAGVRQREGESRLDLVQRNTDVLKQIIPQLIKYSPDTILVIASNPVD 473
DY + V+TAG Q+ GE+R+DL++ N + K+I+ ++ KY+ D IL++A+NPVD
Sbjct: 81 DYKDCKDATAVVITAGKNQKPGETRMDLLKANISIFKEILREVTKYTKDAILLVATNPVD 140
Query: 474 ILTYVTWKISGLPKHRVIGSGTNLDSAR 557
+LTY T K++G P RVIGSGT +D+AR
Sbjct: 141 VLTYATLKLTGFPAERVIGSGTIIDTAR 168
>SPBC1539.07c |||glutathione-dependent formaldehyde dehydrogenase
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 378
Score = 30.7 bits (66), Expect = 0.17
Identities = 23/74 (31%), Positives = 38/74 (51%), Gaps = 1/74 (1%)
Frame = +3
Query: 87 HEKVDETWSKVTIVGVGQVGMAAAFSMLTQNVTNNIALVDMMADKLKGEMMDLQHGSA-F 263
H E+ S V +VG G VG+AA + + IA +D+ ADK E+ + G+ F
Sbjct: 187 HSAKVESGSTVAVVGCGCVGLAAMQGAVAAGASRIIA-IDINADK---EVYAKKFGATDF 242
Query: 264 MRNAKIQSSTDYSI 305
+ ++K++ Y I
Sbjct: 243 IDSSKVKDLVQYVI 256
>SPBC1347.04 |tim54||TIM22 inner membrane protein import complex
subunit Tim54|Schizosaccharomyces pombe|chr 2|||Manual
Length = 347
Score = 27.9 bits (59), Expect = 1.2
Identities = 12/48 (25%), Positives = 21/48 (43%)
Frame = -2
Query: 506 PANLPRNIRQNIHGVTGDHQYCVRTVLYQLRDYLFKHIGVSLHEIETR 363
P LPR ++ +HG GD Y R + +F + +E++
Sbjct: 56 PLELPRKLKVYLHGPPGDGIYVAREEFEEYIRPIFNAAAIEFETVESK 103
>SPAC10F6.09c |psm3|smc3|mitotic cohesin complex subunit
Psm3|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1194
Score = 27.1 bits (57), Expect = 2.1
Identities = 11/38 (28%), Positives = 21/38 (55%)
Frame = +3
Query: 216 DKLKGEMMDLQHGSAFMRNAKIQSSTDYSITAGSKICV 329
+++K E+ +L H +R K Q+ DY+ SK+ +
Sbjct: 268 ERIKAEITELNHSLELLRVEKQQNDEDYTNIMKSKVAL 305
>SPAC821.09 |eng1||endo-1,3-beta-glucanase Eng1|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1016
Score = 25.8 bits (54), Expect = 4.8
Identities = 12/40 (30%), Positives = 21/40 (52%)
Frame = -3
Query: 445 IVSGLYFISCGIICLSTSVFLCTRSRRDSPSRCRTPAVTT 326
I+ G+ ++SC C + S ++C+ SP+ T TT
Sbjct: 901 IIDGVDYLSCNGACYNPSQYVCSDGSL-SPNTVTTTKATT 939
>SPAPB1A10.07c |||sphingolipid biosynthesis
protein|Schizosaccharomyces pombe|chr 1|||Manual
Length = 441
Score = 25.8 bits (54), Expect = 4.8
Identities = 10/21 (47%), Positives = 14/21 (66%)
Frame = -2
Query: 263 EC*SVLQVHHLSFQFVSHHVY 201
+C SV+ VH LSF V H++
Sbjct: 87 KCYSVIAVHRLSFTLVMFHLF 107
>SPCC320.03 |||transcription factor |Schizosaccharomyces pombe|chr
3|||Manual
Length = 867
Score = 25.4 bits (53), Expect = 6.4
Identities = 9/45 (20%), Positives = 23/45 (51%)
Frame = +3
Query: 393 DVLKQIIPQLIKYSPDTILVIASNPVDILTYVTWKISGLPKHRVI 527
D + ++P + + V +++P + +Y T SG+ +H ++
Sbjct: 333 DRVSNVLPSITSFDSSVTTVPSNSPATLNSYTTSVPSGMSRHPML 377
>SPBC16A3.07c |nrm1||negative regulator of MBF|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 342
Score = 25.4 bits (53), Expect = 6.4
Identities = 20/72 (27%), Positives = 35/72 (48%), Gaps = 4/72 (5%)
Frame = -2
Query: 347 SNTSSNHADLRASCYRIIRTRLDLGV----PHEC*SVLQVHHLSFQFVSHHVY*SDVIRN 180
+NT++NH D C + +R RL+L + ++ S LQ + + H+ S+ + +
Sbjct: 120 TNTNNNHLDDIQCCAKNLRLRLELAMYKVQVNQTFSPLQDLPIVAKTKLHNCPNSEPVTS 179
Query: 179 ILRQHRESCGHP 144
I Q S G P
Sbjct: 180 IWNQRSLSSGKP 191
>SPAC664.10 |klp2||kinesin-like protein Klp2|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 817
Score = 25.4 bits (53), Expect = 6.4
Identities = 15/40 (37%), Positives = 20/40 (50%), Gaps = 1/40 (2%)
Frame = +3
Query: 441 TILVIASNPVDILTYVTWKISGLPKHR-VIGSGTNLDSAR 557
TI I S P+D VTW + K+R V + N S+R
Sbjct: 644 TITNITSEPLDTPEQVTWLLDQASKNRSVAATNANEHSSR 683
>SPAC3H8.05c |||conserved fungal protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1073
Score = 25.0 bits (52), Expect = 8.5
Identities = 14/50 (28%), Positives = 24/50 (48%)
Frame = +3
Query: 399 LKQIIPQLIKYSPDTILVIASNPVDILTYVTWKISGLPKHRVIGSGTNLD 548
L I L K + ++I+SN + YVT + G+P +V + + D
Sbjct: 611 LPHSISYLRKSNRAVYVLISSNSTLLTVYVTLTLEGVPDFKVYSNPISTD 660
>SPCC24B10.20 |||short chain dehydrogenase |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 254
Score = 25.0 bits (52), Expect = 8.5
Identities = 18/58 (31%), Positives = 26/58 (44%), Gaps = 2/58 (3%)
Frame = +3
Query: 63 LKKLFQPVHEKVDETW-SKVTIVGVGQVGMAAAF-SMLTQNVTNNIALVDMMADKLKG 230
+ K FQPV + DE W S +G + + AF +L + NI MA + G
Sbjct: 93 ISKSFQPVLKTSDELWMSHYQTNVLGPIHVYQAFYHLLKEGKLKNIVFTSSMAACMGG 150
>SPBC146.12 |coq6||monooxygenase Coq6|Schizosaccharomyces pombe|chr
2|||Manual
Length = 466
Score = 25.0 bits (52), Expect = 8.5
Identities = 16/48 (33%), Positives = 27/48 (56%), Gaps = 2/48 (4%)
Frame = +3
Query: 90 EKVDETWSKVTIVGVGQVGMAAAFSMLTQNVTNN--IALVDMMADKLK 227
+K+ + V IVG G VG+A A + + VT + + L+D+ D +K
Sbjct: 18 QKILQRQFDVVIVGSGPVGLALAAGLQSNPVTQSLKVGLLDIQ-DTMK 64
>SPAC3H1.06c |||membrane transporter |Schizosaccharomyces pombe|chr
1|||Manual
Length = 589
Score = 25.0 bits (52), Expect = 8.5
Identities = 18/52 (34%), Positives = 30/52 (57%), Gaps = 3/52 (5%)
Frame = -3
Query: 472 STGLLAITNIVSGLYFISCGIICLSTSV---FLCTRSRRDSPSRCRTPAVTT 326
+TG A N++ L F GI+C++ V F TR+R +PS +T ++T+
Sbjct: 306 TTGHWAHANVLCYLIF---GILCIAGFVVNEFYTTRTRIITPSAFKTLSLTS 354
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,322,709
Number of Sequences: 5004
Number of extensions: 46294
Number of successful extensions: 142
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 138
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 141
length of database: 2,362,478
effective HSP length: 69
effective length of database: 2,017,202
effective search space used: 262236260
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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