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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= brP-0302
         (600 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At4g17260.1 68417.m02595 L-lactate dehydrogenase, putative stron...   202   1e-52
At1g53240.1 68414.m06033 malate dehydrogenase [NAD], mitochondri...    47   8e-06
At3g15020.1 68416.m01900 malate dehydrogenase [NAD], mitochondri...    46   3e-05
At5g13230.1 68418.m01520 pentatricopeptide (PPR) repeat-containi...    31   0.44 
At2g09900.1 68415.m01028 hypothetical protein                          31   0.58 
At3g47030.1 68416.m05107 F-box family protein contains F-box dom...    29   2.4  
At4g08113.1 68417.m01331 myosin heavy chain-related similar to M...    28   5.4  
At2g41360.1 68415.m05105 F-box family protein similar to SKP1 in...    28   5.4  
At1g51460.1 68414.m05792 ABC transporter family protein similar ...    28   5.4  
At5g53460.1 68418.m06644 glutamate synthase [NADH], chloroplast,...    27   7.2  
At2g36720.1 68415.m04505 PHD finger transcription factor, putative     27   9.5  

>At4g17260.1 68417.m02595 L-lactate dehydrogenase, putative strong
           similarity to L-lactate dehydrogenase from Lycopersicon
           esculentum (GI:1620970, GI:1620972), Hordeum vulgare
           (SP|P22988, SP|P22989); contains InterPro entry
           IPR001236: Lactate/malate dehydrogenase
          Length = 353

 Score =  202 bits (493), Expect = 1e-52
 Identities = 96/165 (58%), Positives = 124/165 (75%), Gaps = 4/165 (2%)
 Frame = +3

Query: 75  FQPVHEKVDETWS----KVTIVGVGQVGMAAAFSMLTQNVTNNIALVDMMADKLKGEMMD 242
           F+P+H       S    KV++VGVG VGMA A ++LTQ++ + IALVD   DKL+GEM+D
Sbjct: 25  FKPIHNSDPSLPSNRRTKVSVVGVGNVGMAIAQTILTQDLADEIALVDAKPDKLRGEMLD 84

Query: 243 LQHGSAFMRNAKIQSSTDYSITAGSKICVVTAGVRQREGESRLDLVQRNTDVLKQIIPQL 422
           LQH +AF+   KI +S DY +TAGS +C+VTAG RQ  GESRL+L+QRN  + + IIP L
Sbjct: 85  LQHAAAFLPRTKITASVDYEVTAGSDLCIVTAGARQNPGESRLNLLQRNVALFRHIIPPL 144

Query: 423 IKYSPDTILVIASNPVDILTYVTWKISGLPKHRVIGSGTNLDSAR 557
            K SPD+IL+I SNPVD+LTYV WK+SG P +RV+GSGTNLDS+R
Sbjct: 145 AKASPDSILIIVSNPVDVLTYVAWKLSGFPVNRVLGSGTNLDSSR 189


>At1g53240.1 68414.m06033 malate dehydrogenase [NAD], mitochondrial
           identical to mitochondrial NAD-dependent malate
           dehydrogenase GI:3929649 SP|Q9ZP06 from [Arabidopsis
           thaliana]; contains InterPro entry IPR001236:
           Lactate/malate dehydrogenase
          Length = 341

 Score = 47.2 bits (107), Expect = 8e-06
 Identities = 38/162 (23%), Positives = 72/162 (44%), Gaps = 5/162 (3%)
 Frame = +3

Query: 114 KVTIVGV-GQVGMAAAFSMLTQNVTNNIALVDMMADKLKGEMMDLQHGSAFMRNAKIQSS 290
           KV I+G  G +G   A  M    + ++++L D+      G   D+ H +           
Sbjct: 31  KVAILGAAGGIGQPLALLMKLNPLVSSLSLYDIA--NTPGVAADVGHINTRSEVVGYMGD 88

Query: 291 TDYS-ITAGSKICVVTAGVRQREGESRLDLVQRNTDVLKQIIPQLIKYSPDTILVIASNP 467
            + +    G+ + ++ AGV ++ G +R DL   N  ++K +   + KY P  ++ + SNP
Sbjct: 89  DNLAKALEGADLVIIPAGVPRKPGMTRDDLFNINAGIVKNLCTAIAKYCPHALINMISNP 148

Query: 468 VDILTYVT---WKISGLPKHRVIGSGTNLDSARVPLPAXGQA 584
           V+    +    +K +G+   + +   T LD  R      G+A
Sbjct: 149 VNSTVPIAAEIFKKAGMYDEKKLFGVTTLDVVRARTFYAGKA 190


>At3g15020.1 68416.m01900 malate dehydrogenase [NAD], mitochondrial,
           putative similar to mitochondrial NAD-dependent malate
           dehydrogenase GB:CAA10320 SP|Q9ZP06 [Arabidopsis
           thaliana]; contains InterPro entry IPR001236:
           Lactate/malate dehydrogenase
          Length = 341

 Score = 45.6 bits (103), Expect = 3e-05
 Identities = 36/153 (23%), Positives = 69/153 (45%), Gaps = 5/153 (3%)
 Frame = +3

Query: 114 KVTIVGV-GQVGMAAAFSMLTQNVTNNIALVDMMADKLKGEMMDLQHGSAFMRNAKIQSS 290
           KV I+G  G +G   +  M    + ++++L D+      G   D+ H +   + +     
Sbjct: 31  KVVILGAAGGIGQPLSLLMKLNPLVSSLSLYDIA--NTPGVAADVGHINTRSQVSGYMGD 88

Query: 291 TDYS-ITAGSKICVVTAGVRQREGESRLDLVQRNTDVLKQIIPQLIKYSPDTILVIASNP 467
            D      G+ + ++ AGV ++ G +R DL   N  ++K +   + KY P  ++ + SNP
Sbjct: 89  DDLGKALEGADLVIIPAGVPRKPGMTRDDLFNINAGIVKNLSIAIAKYCPQALVNMISNP 148

Query: 468 VDILTYVT---WKISGLPKHRVIGSGTNLDSAR 557
           V+    +    +K +G    + +   T LD  R
Sbjct: 149 VNSTVPIAAEIFKKAGTYDEKKLFGVTTLDVVR 181


>At5g13230.1 68418.m01520 pentatricopeptide (PPR) repeat-containing
           protein contains Pfam profile PF01535: PPR repeat
          Length = 822

 Score = 31.5 bits (68), Expect = 0.44
 Identities = 19/66 (28%), Positives = 33/66 (50%), Gaps = 1/66 (1%)
 Frame = +3

Query: 51  KMESLKKLFQPVHEKVDETWSKVTIVGVGQVGMAA-AFSMLTQNVTNNIALVDMMADKLK 227
           KM++  KLF  +  K + +W+ V IVG   +G    AFSM  + + N +++ ++      
Sbjct: 398 KMDTAVKLFAELSSKNEVSWNTV-IVGYENLGEGGKAFSMFREALRNQVSVTEVTFSSAL 456

Query: 228 GEMMDL 245
           G    L
Sbjct: 457 GACASL 462


>At2g09900.1 68415.m01028 hypothetical protein
          Length = 175

 Score = 31.1 bits (67), Expect = 0.58
 Identities = 18/41 (43%), Positives = 25/41 (60%), Gaps = 1/41 (2%)
 Frame = -2

Query: 395 IGVSLHEIETRFTFALSNTSSNHADLRASCY-RIIRTRLDL 276
           I  SLH   TR +  +S T S+H+ +R+S +  IIR  LDL
Sbjct: 30  ITTSLHHSTTRSSALISITRSHHSTMRSSVFTSIIRQPLDL 70


>At3g47030.1 68416.m05107 F-box family protein contains F-box domain
           Pfam:PF00646
          Length = 414

 Score = 29.1 bits (62), Expect = 2.4
 Identities = 16/58 (27%), Positives = 29/58 (50%)
 Frame = -2

Query: 500 NLPRNIRQNIHGVTGDHQYCVRTVLYQLRDYLFKHIGVSLHEIETRFTFALSNTSSNH 327
           NLP +I  +I G+T   +  + T   +   Y+F +  V+L  ++ R  F +    +NH
Sbjct: 330 NLPADIDVSIVGMTSAGEIVLATSHIRHPFYIFYYSTVTLAIVQLRIDFGIEAPEANH 387


>At4g08113.1 68417.m01331 myosin heavy chain-related similar to
           Myosin heavy chain, skeletal muscle, extraocular
           (MyHC-eo) (SP:Q9UKX3)  {Homo sapiens}
          Length = 764

 Score = 27.9 bits (59), Expect = 5.4
 Identities = 18/51 (35%), Positives = 19/51 (37%)
 Frame = +2

Query: 5   SKHYVTSPTXTLTEPQDGVPEEAVPARAREGGRNLEQGDHCRGRSGWDGRS 157
           S   V SP     EP    PE A  A  R  GR+   G   RG      RS
Sbjct: 395 SDQRVASPREERMEPAAATPEAAASAARRSSGRSHGGGRGSRGGGRSSNRS 445


>At2g41360.1 68415.m05105 F-box family protein similar to SKP1
           interacting partner 6 [Arabidopsis thaliana]
           GI:10716957; contains Pfam profile PF00646: F-box domain
          Length = 373

 Score = 27.9 bits (59), Expect = 5.4
 Identities = 19/49 (38%), Positives = 26/49 (53%), Gaps = 1/49 (2%)
 Frame = -2

Query: 542 VSAGPDHAVLRQPANLPRNIRQNIHGVTGDHQYCVRT-VLYQLRDYLFK 399
           +S  PD  VL   A +PR   +NI  V+   +  VRT  LY++R  L K
Sbjct: 11  LSCLPDEMVLNCLARVPRRYYENISCVSVRLRSLVRTPELYRMRSLLHK 59


>At1g51460.1 68414.m05792 ABC transporter family protein similar to
           SP|Q9UNQ0 ATP-binding cassette, sub-family G, member 2
           (Placenta-specific ATP- binding cassette transporter)
           (Breast cancer resistance protein) {Homo sapiens};
           contains Pfam profile PF00005: ABC transporter
          Length = 678

 Score = 27.9 bits (59), Expect = 5.4
 Identities = 11/35 (31%), Positives = 18/35 (51%)
 Frame = +3

Query: 426 KYSPDTILVIASNPVDILTYVTWKISGLPKHRVIG 530
           ++ PD  +V    PV  + Y  W + G  K+ +IG
Sbjct: 535 RFFPDLPMVFWRYPVSYINYGAWALQGAYKNEMIG 569


>At5g53460.1 68418.m06644 glutamate synthase [NADH], chloroplast,
            putative similar to SP|Q03460 Glutamate synthase [NADH],
            chloroplast precursor (EC 1.4.1.14) (NADH- GOGAT)
            {Medicago sativa}
          Length = 2208

 Score = 27.5 bits (58), Expect = 7.2
 Identities = 20/59 (33%), Positives = 30/59 (50%)
 Frame = +3

Query: 105  TWSKVTIVGVGQVGMAAAFSMLTQNVTNNIALVDMMADKLKGEMMDLQHGSAFMRNAKI 281
            T  KV I+G G  G+AAA  +   N   ++  V   +D++ G MM   +G   M+  KI
Sbjct: 1844 TGKKVAIIGSGPAGLAAADQL---NKMGHLVTVYERSDRIGGLMM---YGVPNMKTDKI 1896


>At2g36720.1 68415.m04505 PHD finger transcription factor, putative
          Length = 1007

 Score = 27.1 bits (57), Expect = 9.5
 Identities = 12/35 (34%), Positives = 20/35 (57%)
 Frame = -2

Query: 410 YLFKHIGVSLHEIETRFTFALSNTSSNHADLRASC 306
           Y++   GVSLHE  T F+     +++++ DL   C
Sbjct: 587 YIYTSNGVSLHEWATTFSHGRKYSANDNNDLCVIC 621


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 12,521,631
Number of Sequences: 28952
Number of extensions: 255429
Number of successful extensions: 789
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 766
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 789
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1187288784
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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