BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= brP-0302 (600 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At4g17260.1 68417.m02595 L-lactate dehydrogenase, putative stron... 202 1e-52 At1g53240.1 68414.m06033 malate dehydrogenase [NAD], mitochondri... 47 8e-06 At3g15020.1 68416.m01900 malate dehydrogenase [NAD], mitochondri... 46 3e-05 At5g13230.1 68418.m01520 pentatricopeptide (PPR) repeat-containi... 31 0.44 At2g09900.1 68415.m01028 hypothetical protein 31 0.58 At3g47030.1 68416.m05107 F-box family protein contains F-box dom... 29 2.4 At4g08113.1 68417.m01331 myosin heavy chain-related similar to M... 28 5.4 At2g41360.1 68415.m05105 F-box family protein similar to SKP1 in... 28 5.4 At1g51460.1 68414.m05792 ABC transporter family protein similar ... 28 5.4 At5g53460.1 68418.m06644 glutamate synthase [NADH], chloroplast,... 27 7.2 At2g36720.1 68415.m04505 PHD finger transcription factor, putative 27 9.5 >At4g17260.1 68417.m02595 L-lactate dehydrogenase, putative strong similarity to L-lactate dehydrogenase from Lycopersicon esculentum (GI:1620970, GI:1620972), Hordeum vulgare (SP|P22988, SP|P22989); contains InterPro entry IPR001236: Lactate/malate dehydrogenase Length = 353 Score = 202 bits (493), Expect = 1e-52 Identities = 96/165 (58%), Positives = 124/165 (75%), Gaps = 4/165 (2%) Frame = +3 Query: 75 FQPVHEKVDETWS----KVTIVGVGQVGMAAAFSMLTQNVTNNIALVDMMADKLKGEMMD 242 F+P+H S KV++VGVG VGMA A ++LTQ++ + IALVD DKL+GEM+D Sbjct: 25 FKPIHNSDPSLPSNRRTKVSVVGVGNVGMAIAQTILTQDLADEIALVDAKPDKLRGEMLD 84 Query: 243 LQHGSAFMRNAKIQSSTDYSITAGSKICVVTAGVRQREGESRLDLVQRNTDVLKQIIPQL 422 LQH +AF+ KI +S DY +TAGS +C+VTAG RQ GESRL+L+QRN + + IIP L Sbjct: 85 LQHAAAFLPRTKITASVDYEVTAGSDLCIVTAGARQNPGESRLNLLQRNVALFRHIIPPL 144 Query: 423 IKYSPDTILVIASNPVDILTYVTWKISGLPKHRVIGSGTNLDSAR 557 K SPD+IL+I SNPVD+LTYV WK+SG P +RV+GSGTNLDS+R Sbjct: 145 AKASPDSILIIVSNPVDVLTYVAWKLSGFPVNRVLGSGTNLDSSR 189 >At1g53240.1 68414.m06033 malate dehydrogenase [NAD], mitochondrial identical to mitochondrial NAD-dependent malate dehydrogenase GI:3929649 SP|Q9ZP06 from [Arabidopsis thaliana]; contains InterPro entry IPR001236: Lactate/malate dehydrogenase Length = 341 Score = 47.2 bits (107), Expect = 8e-06 Identities = 38/162 (23%), Positives = 72/162 (44%), Gaps = 5/162 (3%) Frame = +3 Query: 114 KVTIVGV-GQVGMAAAFSMLTQNVTNNIALVDMMADKLKGEMMDLQHGSAFMRNAKIQSS 290 KV I+G G +G A M + ++++L D+ G D+ H + Sbjct: 31 KVAILGAAGGIGQPLALLMKLNPLVSSLSLYDIA--NTPGVAADVGHINTRSEVVGYMGD 88 Query: 291 TDYS-ITAGSKICVVTAGVRQREGESRLDLVQRNTDVLKQIIPQLIKYSPDTILVIASNP 467 + + G+ + ++ AGV ++ G +R DL N ++K + + KY P ++ + SNP Sbjct: 89 DNLAKALEGADLVIIPAGVPRKPGMTRDDLFNINAGIVKNLCTAIAKYCPHALINMISNP 148 Query: 468 VDILTYVT---WKISGLPKHRVIGSGTNLDSARVPLPAXGQA 584 V+ + +K +G+ + + T LD R G+A Sbjct: 149 VNSTVPIAAEIFKKAGMYDEKKLFGVTTLDVVRARTFYAGKA 190 >At3g15020.1 68416.m01900 malate dehydrogenase [NAD], mitochondrial, putative similar to mitochondrial NAD-dependent malate dehydrogenase GB:CAA10320 SP|Q9ZP06 [Arabidopsis thaliana]; contains InterPro entry IPR001236: Lactate/malate dehydrogenase Length = 341 Score = 45.6 bits (103), Expect = 3e-05 Identities = 36/153 (23%), Positives = 69/153 (45%), Gaps = 5/153 (3%) Frame = +3 Query: 114 KVTIVGV-GQVGMAAAFSMLTQNVTNNIALVDMMADKLKGEMMDLQHGSAFMRNAKIQSS 290 KV I+G G +G + M + ++++L D+ G D+ H + + + Sbjct: 31 KVVILGAAGGIGQPLSLLMKLNPLVSSLSLYDIA--NTPGVAADVGHINTRSQVSGYMGD 88 Query: 291 TDYS-ITAGSKICVVTAGVRQREGESRLDLVQRNTDVLKQIIPQLIKYSPDTILVIASNP 467 D G+ + ++ AGV ++ G +R DL N ++K + + KY P ++ + SNP Sbjct: 89 DDLGKALEGADLVIIPAGVPRKPGMTRDDLFNINAGIVKNLSIAIAKYCPQALVNMISNP 148 Query: 468 VDILTYVT---WKISGLPKHRVIGSGTNLDSAR 557 V+ + +K +G + + T LD R Sbjct: 149 VNSTVPIAAEIFKKAGTYDEKKLFGVTTLDVVR 181 >At5g13230.1 68418.m01520 pentatricopeptide (PPR) repeat-containing protein contains Pfam profile PF01535: PPR repeat Length = 822 Score = 31.5 bits (68), Expect = 0.44 Identities = 19/66 (28%), Positives = 33/66 (50%), Gaps = 1/66 (1%) Frame = +3 Query: 51 KMESLKKLFQPVHEKVDETWSKVTIVGVGQVGMAA-AFSMLTQNVTNNIALVDMMADKLK 227 KM++ KLF + K + +W+ V IVG +G AFSM + + N +++ ++ Sbjct: 398 KMDTAVKLFAELSSKNEVSWNTV-IVGYENLGEGGKAFSMFREALRNQVSVTEVTFSSAL 456 Query: 228 GEMMDL 245 G L Sbjct: 457 GACASL 462 >At2g09900.1 68415.m01028 hypothetical protein Length = 175 Score = 31.1 bits (67), Expect = 0.58 Identities = 18/41 (43%), Positives = 25/41 (60%), Gaps = 1/41 (2%) Frame = -2 Query: 395 IGVSLHEIETRFTFALSNTSSNHADLRASCY-RIIRTRLDL 276 I SLH TR + +S T S+H+ +R+S + IIR LDL Sbjct: 30 ITTSLHHSTTRSSALISITRSHHSTMRSSVFTSIIRQPLDL 70 >At3g47030.1 68416.m05107 F-box family protein contains F-box domain Pfam:PF00646 Length = 414 Score = 29.1 bits (62), Expect = 2.4 Identities = 16/58 (27%), Positives = 29/58 (50%) Frame = -2 Query: 500 NLPRNIRQNIHGVTGDHQYCVRTVLYQLRDYLFKHIGVSLHEIETRFTFALSNTSSNH 327 NLP +I +I G+T + + T + Y+F + V+L ++ R F + +NH Sbjct: 330 NLPADIDVSIVGMTSAGEIVLATSHIRHPFYIFYYSTVTLAIVQLRIDFGIEAPEANH 387 >At4g08113.1 68417.m01331 myosin heavy chain-related similar to Myosin heavy chain, skeletal muscle, extraocular (MyHC-eo) (SP:Q9UKX3) {Homo sapiens} Length = 764 Score = 27.9 bits (59), Expect = 5.4 Identities = 18/51 (35%), Positives = 19/51 (37%) Frame = +2 Query: 5 SKHYVTSPTXTLTEPQDGVPEEAVPARAREGGRNLEQGDHCRGRSGWDGRS 157 S V SP EP PE A A R GR+ G RG RS Sbjct: 395 SDQRVASPREERMEPAAATPEAAASAARRSSGRSHGGGRGSRGGGRSSNRS 445 >At2g41360.1 68415.m05105 F-box family protein similar to SKP1 interacting partner 6 [Arabidopsis thaliana] GI:10716957; contains Pfam profile PF00646: F-box domain Length = 373 Score = 27.9 bits (59), Expect = 5.4 Identities = 19/49 (38%), Positives = 26/49 (53%), Gaps = 1/49 (2%) Frame = -2 Query: 542 VSAGPDHAVLRQPANLPRNIRQNIHGVTGDHQYCVRT-VLYQLRDYLFK 399 +S PD VL A +PR +NI V+ + VRT LY++R L K Sbjct: 11 LSCLPDEMVLNCLARVPRRYYENISCVSVRLRSLVRTPELYRMRSLLHK 59 >At1g51460.1 68414.m05792 ABC transporter family protein similar to SP|Q9UNQ0 ATP-binding cassette, sub-family G, member 2 (Placenta-specific ATP- binding cassette transporter) (Breast cancer resistance protein) {Homo sapiens}; contains Pfam profile PF00005: ABC transporter Length = 678 Score = 27.9 bits (59), Expect = 5.4 Identities = 11/35 (31%), Positives = 18/35 (51%) Frame = +3 Query: 426 KYSPDTILVIASNPVDILTYVTWKISGLPKHRVIG 530 ++ PD +V PV + Y W + G K+ +IG Sbjct: 535 RFFPDLPMVFWRYPVSYINYGAWALQGAYKNEMIG 569 >At5g53460.1 68418.m06644 glutamate synthase [NADH], chloroplast, putative similar to SP|Q03460 Glutamate synthase [NADH], chloroplast precursor (EC 1.4.1.14) (NADH- GOGAT) {Medicago sativa} Length = 2208 Score = 27.5 bits (58), Expect = 7.2 Identities = 20/59 (33%), Positives = 30/59 (50%) Frame = +3 Query: 105 TWSKVTIVGVGQVGMAAAFSMLTQNVTNNIALVDMMADKLKGEMMDLQHGSAFMRNAKI 281 T KV I+G G G+AAA + N ++ V +D++ G MM +G M+ KI Sbjct: 1844 TGKKVAIIGSGPAGLAAADQL---NKMGHLVTVYERSDRIGGLMM---YGVPNMKTDKI 1896 >At2g36720.1 68415.m04505 PHD finger transcription factor, putative Length = 1007 Score = 27.1 bits (57), Expect = 9.5 Identities = 12/35 (34%), Positives = 20/35 (57%) Frame = -2 Query: 410 YLFKHIGVSLHEIETRFTFALSNTSSNHADLRASC 306 Y++ GVSLHE T F+ +++++ DL C Sbjct: 587 YIYTSNGVSLHEWATTFSHGRKYSANDNNDLCVIC 621 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 12,521,631 Number of Sequences: 28952 Number of extensions: 255429 Number of successful extensions: 789 Number of sequences better than 10.0: 11 Number of HSP's better than 10.0 without gapping: 766 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 789 length of database: 12,070,560 effective HSP length: 78 effective length of database: 9,812,304 effective search space used: 1187288784 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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