SilkBase IMG001 IMG002 IMG003 IMG005 IMG006 IMG007 IMG008 IMG009 kuwako IMG010 IMG011 IMG012

Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= brP-0290
         (700 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At3g01280.1 68416.m00035 porin, putative similar to SP|P42055 34...    63   2e-10
At5g57490.1 68418.m07184 porin, putative similar to 36kDA porin ...    62   3e-10
At5g67500.1 68418.m08512 porin, putative similar to SP|P42055 34...    53   2e-07
At3g49920.1 68416.m05458 porin, putative similar to SP|P42055 34...    32   0.42 
At2g36340.1 68415.m04460 DNA-binding storekeeper protein-related...    29   2.2  
At3g13610.1 68416.m01713 oxidoreductase, 2OG-Fe(II) oxygenase fa...    29   3.0  
At4g30810.1 68417.m04365 serine carboxypeptidase S10 family prot...    27   9.0  
At4g25920.1 68417.m03727 expressed protein contains Pfam profile...    27   9.0  
At3g19840.1 68416.m02513 FF domain-containing protein / WW domai...    27   9.0  
At3g17365.1 68416.m02219 expressed protein low similarity to PIR...    27   9.0  
At2g04740.1 68415.m00484 ankyrin repeat family protein contains ...    27   9.0  

>At3g01280.1 68416.m00035 porin, putative similar to SP|P42055 34
           kDa outer mitochondrial membrane protein porin
           (Voltage-dependent anion-selective channel protein)
           (VDAC) {Solanum tuberosum}; contains Pfam profile
           PF01459: Eukaryotic porin
          Length = 276

 Score = 62.9 bits (146), Expect = 2e-10
 Identities = 53/204 (25%), Positives = 87/204 (42%), Gaps = 1/204 (0%)
 Frame = +1

Query: 91  PPYYADLGKKANDVFSKGYHFGVFKLDLKTKSESGVEFTSGITSNQESGKVFGSLSSKFA 270
           P  Y ++GKKA D+  K  H    K  + T S +GV  TS  T  ++   + G ++  F 
Sbjct: 5   PGLYTEIGKKARDLLYKD-HNSDQKFSITTFSPAGVAITS--TGTKKGDLLLGDVA--FQ 59

Query: 271 VKDYGLTFTEKWNTDNTLATDITIQDKIAAGLKVTLEGTFAPQTGTKTGKLKTSFTNDTV 450
            +   +T   K  TD+T     T+ D+ A GL+         Q    +GK++  + ++  
Sbjct: 60  SRRKNITTDLKVCTDSTFLITATV-DEAAPGLRSIFSFKVPDQN---SGKVELQYLHEYA 115

Query: 451 AVNTNLDLDLAGPVVDVAAVLNYQGWLAGVHTQFDTQKAKFSKNNFALGYQSGDFALHTN 630
            ++T++ L    P V+ + V+       G    FDT+   F+K N  L +   D      
Sbjct: 116 GISTSMGLT-QNPTVNFSGVIGSNVLAVGTDVSFDTKSGNFTKINAGLSFTKEDLIASLT 174

Query: 631 V-DNGKDFGGSIYQXVSDKLDCGV 699
           V D G     S Y  V+   +  V
Sbjct: 175 VNDKGDLLNASYYHIVNPLFNTAV 198


>At5g57490.1 68418.m07184 porin, putative similar to 36kDA porin II
           [Solanum tuberosum] GI:515360; contains Pfam profile
           PF01459: Eukaryotic porin
          Length = 274

 Score = 62.1 bits (144), Expect = 3e-10
 Identities = 43/173 (24%), Positives = 86/173 (49%)
 Frame = +1

Query: 88  APPYYADLGKKANDVFSKGYHFGVFKLDLKTKSESGVEFTSGITSNQESGKVFGSLSSKF 267
           +P  +AD+GKKA D+ +K Y F   K  L   S +G EF +  T  ++    FG +S+ +
Sbjct: 4   SPAPFADIGKKAKDLLNKDYIFD-HKFTLTMLSATGTEFVA--TGLKKDDFFFGDISTLY 60

Query: 268 AVKDYGLTFTEKWNTDNTLATDITIQDKIAAGLKVTLEGTFAPQTGTKTGKLKTSFTNDT 447
             K        K ++ ++++T +T+++ + +  K  +          K+GKL   + +  
Sbjct: 61  --KGQNTIVDLKIDSHSSVSTKVTLKNLLPSA-KAVISFKIPDH---KSGKLDVQYVHPH 114

Query: 448 VAVNTNLDLDLAGPVVDVAAVLNYQGWLAGVHTQFDTQKAKFSKNNFALGYQS 606
             +N+++ L+   P++D++A +  Q    G    FDT  +  +K N  +G+ +
Sbjct: 115 ATLNSSIGLN-PTPLLDLSATIGSQNVCLGGEVSFDTASSSLTKYNAGIGFNN 166


>At5g67500.1 68418.m08512 porin, putative similar to SP|P42055 34
           kDa outer mitochondrial membrane protein porin
           (Voltage-dependent anion-selective channel protein)
           (VDAC) {Solanum tuberosum}; contains Pfam profile
           PF01459: Eukaryotic porin
          Length = 276

 Score = 52.8 bits (121), Expect = 2e-07
 Identities = 45/177 (25%), Positives = 79/177 (44%)
 Frame = +1

Query: 91  PPYYADLGKKANDVFSKGYHFGVFKLDLKTKSESGVEFTSGITSNQESGKVFGSLSSKFA 270
           P  + D+GKKA D+ ++ Y+    K  + T S SGV  TS  T+ ++ G     +++++ 
Sbjct: 5   PGLFTDIGKKAKDLLTRDYNSDQ-KFSISTYSASGVALTS--TALKKGGVHAADVATQYK 61

Query: 271 VKDYGLTFTEKWNTDNTLATDITIQDKIAAGLKVTLEGTFAPQTGTKTGKLKTSFTNDTV 450
            K+    F  K +TD+++ T +T+ + I    K  +     P     + KL+  + +D  
Sbjct: 62  YKN--ALFDVKIDTDSSVLTTVTLTE-ILPSTK-AIASFKVPD--YNSAKLEVQYFHDHA 115

Query: 451 AVNTNLDLDLAGPVVDVAAVLNYQGWLAGVHTQFDTQKAKFSKNNFALGYQSGDFAL 621
            V     L    P++D+ A L       G    +DT    F+K N  +     D  L
Sbjct: 116 TVTAAAALK-QNPLIDITATLGSPVISFGAEAGYDTTSKTFTKYNAGISVTKPDACL 171


>At3g49920.1 68416.m05458 porin, putative similar to SP|P42055 34
           kDa outer mitochondrial membrane protein porin
           (Voltage-dependent anion-selective channel protein)
           (VDAC) {Solanum tuberosum}; contains Pfam profile
           PF01459: Eukaryotic porin
          Length = 226

 Score = 31.9 bits (69), Expect = 0.42
 Identities = 17/45 (37%), Positives = 23/45 (51%)
 Frame = +1

Query: 91  PPYYADLGKKANDVFSKGYHFGVFKLDLKTKSESGVEFTSGITSN 225
           P  +AD+GK A D+ ++ Y     K  + T S SGV  TS    N
Sbjct: 5   PGLFADIGKYAKDLLTRDYSTDQ-KFSISTNSVSGVALTSTALKN 48


>At2g36340.1 68415.m04460 DNA-binding storekeeper protein-related
           contains similarity to storekeeper protein [Solanum
           tuberosum] gi|14268476|emb|CAC39398; contains PF04504:
           Protein of unknown function, DUF573
          Length = 414

 Score = 29.5 bits (63), Expect = 2.2
 Identities = 26/94 (27%), Positives = 43/94 (45%), Gaps = 3/94 (3%)
 Frame = +1

Query: 307 NTDNTLATDITIQDKIAAGLKVTLEGTFAPQTGTKTGKLKTSFTNDTVAVNTNLDLDLAG 486
           N+D+TL  +  ++ +     +  L  T    T T+T  L  S +   +  + N +L + G
Sbjct: 14  NSDDTLTRNREVEVEAMLSRRKQLRTTTTRTTTTRTTPLSLSSSASKMNWSKNDELVILG 73

Query: 487 PVVDV--AAVLNYQG-WLAGVHTQFDTQKAKFSK 579
            +VD      L+Y+  W A      D  +AKFSK
Sbjct: 74  GIVDYENETKLSYRSDWDALYRYIKDCVEAKFSK 107


>At3g13610.1 68416.m01713 oxidoreductase, 2OG-Fe(II) oxygenase
           family protein similar to desacetoxyvindoline
           4-hydroxylase [Catharanthus roseus][GI:1916643],
           flavonol synthase 1 [SP|Q96330]; contains PF03171
           2OG-Fe(II) oxygenase superfamily domain
          Length = 361

 Score = 29.1 bits (62), Expect = 3.0
 Identities = 18/59 (30%), Positives = 27/59 (45%), Gaps = 1/59 (1%)
 Frame = -3

Query: 362 PAAILSWIVMSVANVLSVFHFSVKVKP*SFTANLEERLPKTFPLSWLEVIPLVN-STPD 189
           PA +  ++V     V  +    +K  P  +   LEERL   F     E IP+++ S PD
Sbjct: 13  PAEVTDFVVYKGNGVKGLSETGIKALPEQYIQPLEERLINKFVNETDEAIPVIDMSNPD 71


>At4g30810.1 68417.m04365 serine carboxypeptidase S10 family protein
           similar to serine-type carboxypeptidase (SP:P55748)
           [Hordeum vulgare]
          Length = 479

 Score = 27.5 bits (58), Expect = 9.0
 Identities = 14/37 (37%), Positives = 21/37 (56%)
 Frame = -2

Query: 291 SQAIVFHCKFGGKAAKNLSAFLVGGDPAGEFNTRLAL 181
           S+AIV H +   K + NL  ++VG     +F+ RL L
Sbjct: 196 SEAIVKHNQGSDKNSINLKGYMVGNGLMDDFHDRLGL 232


>At4g25920.1 68417.m03727 expressed protein contains Pfam profile
           PF03478: Protein of unknown function (DUF295)
          Length = 390

 Score = 27.5 bits (58), Expect = 9.0
 Identities = 12/29 (41%), Positives = 17/29 (58%)
 Frame = +1

Query: 94  PYYADLGKKANDVFSKGYHFGVFKLDLKT 180
           P  +  G K N ++  GY+FGV+ L  KT
Sbjct: 330 PASSSPGLKPNCIYFVGYNFGVYDLTTKT 358


>At3g19840.1 68416.m02513 FF domain-containing protein / WW
           domain-containing protein weak similarity to
           transcription factor CA150b [Mus musculus] GI:6329166;
           contains Pfam profiles PF01846: FF domain, PF00397: WW
           domain
          Length = 743

 Score = 27.5 bits (58), Expect = 9.0
 Identities = 20/59 (33%), Positives = 31/59 (52%), Gaps = 2/59 (3%)
 Frame = +1

Query: 70  TQLKDMAPPYYADLGKKANDVFSKGYHFGVFKLDLKTKS--ESGVEFTSGITSNQESGK 240
           + L  ++ P  ++ G+ A  +  K  +FG   LDL  K   +SG+  +S ITS   SGK
Sbjct: 265 SDLTSLSAPAISNGGRDAASL--KTTNFGSSALDLVKKKLHDSGMPVSSTITSEANSGK 321


>At3g17365.1 68416.m02219 expressed protein low similarity to
           PIR|I46078 endothelin converting enzyme from Bos
           primigenius taurus
          Length = 239

 Score = 27.5 bits (58), Expect = 9.0
 Identities = 14/46 (30%), Positives = 21/46 (45%)
 Frame = -1

Query: 622 GEQSHQIGNLEQSCSWRTLLFVYQTGCVHQPANPGSLVLLQRLQLD 485
           G   +++   ++SCSW T L V       QP +     L + L LD
Sbjct: 159 GAPIYRLRLFKESCSWTTKLHVIDKSLTDQPLDTPKWELTKPLPLD 204


>At2g04740.1 68415.m00484 ankyrin repeat family protein contains
           ankyrin repeats, Pfam:PF00023
          Length = 578

 Score = 27.5 bits (58), Expect = 9.0
 Identities = 16/55 (29%), Positives = 28/55 (50%), Gaps = 1/55 (1%)
 Frame = -3

Query: 419 FPVLVPVCGAKVPSRVTLRPAAILSWIVMS-VANVLSVFHFSVKVKP*SFTANLE 258
           FP+   V  A +P   T  PA +  W+V+S +  VL +  + + +   +F A +E
Sbjct: 431 FPLKRAVADALLPHLETATPAELCQWLVLSDMYGVLKIREYCLDLVACNFEAFVE 485


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 14,916,535
Number of Sequences: 28952
Number of extensions: 309421
Number of successful extensions: 841
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 809
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 839
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1496852856
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

- SilkBase 1999-2023 -