Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= brP-0189
(823 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 387 e-108
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 384 e-107
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 196 2e-51
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 158 1e-39
SPBC1718.06 |msp1|mgm1|mitochondrial GTPase Msp1|Schizosaccharom... 28 1.4
SPBC146.13c |myo1||myosin type I|Schizosaccharomyces pombe|chr 2... 27 3.2
SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||... 26 7.4
SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr... 26 7.4
SPCC16A11.08 |atg20||sorting nexin Atg20|Schizosaccharomyces pom... 25 9.8
>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 449
Score = 387 bits (952), Expect = e-108
Identities = 172/249 (69%), Positives = 207/249 (83%)
Frame = +2
Query: 77 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGK 256
MRE IS+HVGQAG QIGNACWELYCLEHGIQP+G M + D F+TFFSETG GK
Sbjct: 1 MREIISIHVGQAGTQIGNACWELYCLEHGIQPNGYMNPETASQNSDGGFSTFFSETGQGK 60
Query: 257 HVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLD 436
+VPR+++VDLEP V+D+VRTG YR LFHPEQLITGKEDA+NNYARGHYT+GKE+VD V D
Sbjct: 61 YVPRSIYVDLEPNVIDQVRTGPYRDLFHPEQLITGKEDASNNYARGHYTVGKELVDEVTD 120
Query: 437 RIRKLADQCTGLQXFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYPAPXVSTA 616
+IR++AD C+GLQ FL+FH LL+ERL+++Y KKSKL+F++YPAP VST+
Sbjct: 121 KIRRIADNCSGLQGFLVFHSFGGGTGSGFGALLLERLAMEYTKKSKLQFSVYPAPQVSTS 180
Query: 617 VVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLIGQIGSSITA 796
VVEPYNS+LTTH TL+ +DC FMVDNE+ YDICRRNLDIERP+Y NLNRLI Q+ SSITA
Sbjct: 181 VVEPYNSVLTTHATLDLADCTFMVDNESCYDICRRNLDIERPSYENLNRLIAQVVSSITA 240
Query: 797 SLKFDGALN 823
SL+F+G+LN
Sbjct: 241 SLRFEGSLN 249
>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 455
Score = 384 bits (944), Expect = e-107
Identities = 176/254 (69%), Positives = 209/254 (82%), Gaps = 5/254 (1%)
Frame = +2
Query: 77 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTD-----KTIGGGDDSFNTFFSE 241
MRE ISVHVGQAGVQIGNACWELYCLEHGI PDG PT+ K +D F TFFSE
Sbjct: 1 MREVISVHVGQAGVQIGNACWELYCLEHGIGPDG-FPTENSEVHKNNSYLNDGFGTFFSE 59
Query: 242 TGAGKHVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIV 421
TG GK VPR+++VDLEP V+D+VRTG Y+ LFHPEQ++TGKEDA+NNYARGHYT+GKE++
Sbjct: 60 TGQGKFVPRSIYVDLEPNVIDQVRTGPYKDLFHPEQMVTGKEDASNNYARGHYTVGKEMI 119
Query: 422 DLVLDRIRKLADQCTGLQXFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYPAP 601
D VL+RIR++AD C+GLQ FL+FH LL+ERL+++YGKKS L+F++YPAP
Sbjct: 120 DSVLERIRRMADNCSGLQGFLVFHSFGGGTGSGLGALLLERLNMEYGKKSNLQFSVYPAP 179
Query: 602 XVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLIGQIG 781
VST+VVEPYNS+LTTH TL++SDC FMVDNEA YDICRRNLDIERPTY NLNRLI Q+
Sbjct: 180 QVSTSVVEPYNSVLTTHATLDNSDCTFMVDNEACYDICRRNLDIERPTYENLNRLIAQVV 239
Query: 782 SSITASLKFDGALN 823
SSITASL+F G+LN
Sbjct: 240 SSITASLRFAGSLN 253
>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 448
Score = 196 bits (479), Expect = 2e-51
Identities = 96/249 (38%), Positives = 139/249 (55%)
Frame = +2
Query: 77 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGK 256
MRE + + GQ G Q+G A W EHG+ G T + N +F+E GK
Sbjct: 1 MREIVHIQAGQCGNQVGAAFWSTIADEHGLDSAGIY--HGTSEAQHERLNVYFNEAAGGK 58
Query: 257 HVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLD 436
+VPRAV VDLEP +D V++G + LF P+ +I G+ A N +A+GHYT G E+ D VLD
Sbjct: 59 YVPRAVLVDLEPGTMDAVKSGKFGNLFRPDNIIYGQSGAGNIWAKGHYTEGAELADAVLD 118
Query: 437 RIRKLADQCTGLQXFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYPAPXVSTA 616
+R+ A+ C LQ F + H LL+ ++ +Y + F++ PAP S
Sbjct: 119 VVRREAEACDALQGFQLTHSLGGGTGSGMGTLLLSKIREEYPDRMMATFSVAPAPKSSDT 178
Query: 617 VVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLIGQIGSSITA 796
VVEPYN+ L+ H +E+SD F +DNEA+ I L I+ P+Y +LN L+ + + +T
Sbjct: 179 VVEPYNATLSMHQLVENSDETFCIDNEALSSIFANTLKIKSPSYDDLNHLVSAVMAGVTT 238
Query: 797 SLKFDGALN 823
S +F G LN
Sbjct: 239 SFRFPGELN 247
>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 446
Score = 158 bits (383), Expect = 1e-39
Identities = 85/251 (33%), Positives = 139/251 (55%), Gaps = 3/251 (1%)
Frame = +2
Query: 80 RECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGKH 259
RE I++ GQ G QIG+ W+ CLEHGI PDG + + T G D + FF ++ ++
Sbjct: 3 REIITLQAGQCGNQIGSQFWQQLCLEHGIGPDGTLESFAT--EGVDRKDVFFYQSDDTRY 60
Query: 260 VPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKED--AANNYARGHYTIGKEIVDLVL 433
+PRA+ +DLEP VV+ + + TY L++PE ++ K A NN+A G Y+ + I + ++
Sbjct: 61 IPRAILIDLEPRVVNNILSDTYGSLYNPENILITKNGGGAGNNWANG-YSHAERIFEDIM 119
Query: 434 DRIRKLADQCTGLQXFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYP-APXVS 610
D I + AD L+ F + H L+ERL+ Y KK ++++P + VS
Sbjct: 120 DMIDREADGSDSLEGFSLLHSIAGGTGSGLGSFLLERLNDRYPKKIIQTYSVFPNSQSVS 179
Query: 611 TAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLIGQIGSSI 790
VV+PYNS+L ++D ++DN A+ I L + PT+ N+L+ + S+
Sbjct: 180 DVVVQPYNSLLALKRLTLNADSVVVLDNAALAHIAADRLHTQNPTFHQQNQLVSTVMSAS 239
Query: 791 TASLKFDGALN 823
T +L++ G +N
Sbjct: 240 TTTLRYPGYMN 250
>SPBC1718.06 |msp1|mgm1|mitochondrial GTPase
Msp1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 903
Score = 28.3 bits (60), Expect = 1.4
Identities = 15/35 (42%), Positives = 21/35 (60%)
Frame = -1
Query: 802 QRSSDRGANLSDETIQVGVGWALNVEITAADVIDG 698
Q+SSD + +D T VG+G AL I + D +DG
Sbjct: 184 QKSSDNDEDPNDTT--VGIGAALAASILSVDSVDG 216
>SPBC146.13c |myo1||myosin type I|Schizosaccharomyces pombe|chr
2|||Manual
Length = 1217
Score = 27.1 bits (57), Expect = 3.2
Identities = 10/34 (29%), Positives = 21/34 (61%)
Frame = -1
Query: 811 VEFQRSSDRGANLSDETIQVGVGWALNVEITAAD 710
++FQ D G+ +SD++I +G+ + V + A +
Sbjct: 310 IQFQEGQDGGSVISDKSITEFLGYLIGVPVAAIE 343
>SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr
2|||Manual
Length = 534
Score = 25.8 bits (54), Expect = 7.4
Identities = 15/35 (42%), Positives = 19/35 (54%)
Frame = +3
Query: 156 STASSLMARCPQTRPSGVETILSTLSSARPELAST 260
ST SSL + ++PS T ST SSA P S+
Sbjct: 173 STFSSLSSSTSSSQPSVSSTSSSTFSSAAPTSTSS 207
>SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1275
Score = 25.8 bits (54), Expect = 7.4
Identities = 13/22 (59%), Positives = 13/22 (59%)
Frame = +1
Query: 175 WPDAHRQDHRGWRRFFQHFLQR 240
W A R D R R FQHFLQR
Sbjct: 590 WLAACRSDPRCRRLDFQHFLQR 611
>SPCC16A11.08 |atg20||sorting nexin Atg20|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 534
Score = 25.4 bits (53), Expect = 9.8
Identities = 15/41 (36%), Positives = 21/41 (51%)
Frame = +3
Query: 66 LKSKCVSASLYTLAKPESRSVMPAGSFTAWSTASSLMARCP 188
L+S +L+ P SR++ P S + STASSL P
Sbjct: 170 LRSSMPLVMANSLSPPSSRALKPIHSLSNPSTASSLEPSSP 210
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,265,411
Number of Sequences: 5004
Number of extensions: 66788
Number of successful extensions: 203
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 191
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 198
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 402440190
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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