Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= brP-0185
(730 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g20080.1 68414.m02513 C2 domain-containing protein contains I... 62 4e-10
At5g04220.2 68418.m00411 C2 domain-containing protein (sytC) GC ... 59 3e-09
At3g61050.1 68416.m06832 calcium-dependent lipid-binding protein... 59 3e-09
At2g20990.1 68415.m02485 C2 domain-containing protein (sytA) sim... 58 6e-09
At5g11100.1 68418.m01296 C2 domain-containing protein similar to... 49 4e-06
At1g05500.1 68414.m00561 C2 domain-containing protein similar to... 39 0.004
At5g02390.1 68418.m00162 expressed protein ; expression supporte... 29 3.2
At5g35210.2 68418.m04175 peptidase M50 family protein / sterol-r... 28 5.5
At5g35210.1 68418.m04174 peptidase M50 family protein / sterol-r... 28 5.5
At5g28860.1 68418.m03551 hypothetical protein 28 5.5
At1g72840.1 68414.m08425 disease resistance protein (TIR-NBS-LRR... 28 5.5
At1g71691.2 68414.m08276 GDSL-motif lipase/hydrolase family prot... 28 5.5
At1g71691.1 68414.m08275 GDSL-motif lipase/hydrolase family prot... 28 5.5
At5g36930.1 68418.m04427 disease resistance protein (TIR-NBS-LRR... 28 7.3
At5g03280.1 68418.m00277 ethylene-insensitive 2 (EIN2) identical... 28 7.3
At3g61030.1 68416.m06828 C2 domain-containing protein similar to... 28 7.3
At3g60950.1 68416.m06819 C2 domain-containing protein similar to... 28 7.3
At1g69860.1 68414.m08040 proton-dependent oligopeptide transport... 27 9.6
>At1g20080.1 68414.m02513 C2 domain-containing protein contains
INTERPRO:IPR000008 C2 domain
Length = 535
Score = 62.1 bits (144), Expect = 4e-10
Identities = 22/78 (28%), Positives = 44/78 (56%)
Frame = +3
Query: 486 EKDVILARLDDLPSWVFFPDVERAEWLNRILLQVWPNVNSYAKTLLXDLIEPAVAXSLAN 665
+ + I ++P WV PD +R +WLN+++ +WP ++ + + +P +A + N
Sbjct: 46 DSETIATMFPEIPMWVKNPDFDRIDWLNKLIGHMWPYMDKAICKMAKSIAKPIIAEQIPN 105
Query: 666 YKLSGFKFERMILGTIAP 719
YK+ +FE + LG++ P
Sbjct: 106 YKIDSVEFEMLTLGSLPP 123
>At5g04220.2 68418.m00411 C2 domain-containing protein (sytC) GC
donor splice site at exon 3; similar to Ca2+-dependent
lipid-binding protein (CLB1) GI:2789434 from
[Lycopersicon esculentum]
Length = 540
Score = 58.8 bits (136), Expect = 3e-09
Identities = 23/77 (29%), Positives = 42/77 (54%)
Frame = +3
Query: 495 VILARLDDLPSWVFFPDVERAEWLNRILLQVWPNVNSYAKTLLXDLIEPAVAXSLANYKL 674
V+L L D+P W+ PD ER +W N+ + +WP ++ ++ ++P A + + +
Sbjct: 51 VLLDLLPDIPLWMKNPDYERVDWFNKFISYMWPYLDKAVCGIIRSSVQPLFADYIGTFCI 110
Query: 675 SGFKFERMILGTIAPRV 725
+FE + LGT+ P V
Sbjct: 111 ESIEFENLSLGTLPPTV 127
>At3g61050.1 68416.m06832 calcium-dependent lipid-binding protein,
putative strong similarity to CLB1 [Lycopersicon
esculentum] GI:2789434; contains Pfam profile PF00168:
C2 domain
Length = 510
Score = 58.8 bits (136), Expect = 3e-09
Identities = 24/74 (32%), Positives = 45/74 (60%), Gaps = 3/74 (4%)
Frame = +3
Query: 513 DDLPSWVFFPDVERAEWLNRILLQVWPNVNSYAKTLLXDLIEPAVAXSLANYK---LSGF 683
D+ P W+ FP E+ +WLN++L ++WP + A ++ D +EP L +Y+ ++
Sbjct: 56 DNFPQWISFPAFEQVKWLNKLLSKMWPYIAEAATMVIRDSVEPL----LEDYRPPGITSL 111
Query: 684 KFERMILGTIAPRV 725
KF ++ LG +AP++
Sbjct: 112 KFSKLTLGNVAPKI 125
>At2g20990.1 68415.m02485 C2 domain-containing protein (sytA)
similar to Ca2+-dependent lipid-binding protein (CLB1)
GI:2789434 from [Lycopersicon esculentum]
Length = 541
Score = 58.0 bits (134), Expect = 6e-09
Identities = 30/121 (24%), Positives = 58/121 (47%)
Frame = +3
Query: 357 LVGYMQWSVAWLIGPVILSVMRDQWRKENEYKRNIAKVAALSSEKDVILARLDDLPSWVF 536
++G+ + V +G VI V+ + I +A + +L L ++P WV
Sbjct: 7 ILGFCGFGVGISLGLVIGYVLFVYLLPNDVKDPEIRSIA--DQDPKAMLRMLPEIPLWVK 64
Query: 537 FPDVERAEWLNRILLQVWPNVNSYAKTLLXDLIEPAVAXSLANYKLSGFKFERMILGTIA 716
PD +R +W+NR L +WP ++ ++ +P + + YK+ +FE + LG++
Sbjct: 65 NPDFDRVDWINRFLEYMWPYLDKAICKTAKNIAKPIIEEQIPKYKIDSVEFETLTLGSLP 124
Query: 717 P 719
P
Sbjct: 125 P 125
>At5g11100.1 68418.m01296 C2 domain-containing protein similar to
Ca2+-dependent lipid-binding protein (CLB1) GI:2789434
from [Lycopersicon esculentum]
Length = 574
Score = 48.8 bits (111), Expect = 4e-06
Identities = 31/97 (31%), Positives = 45/97 (46%), Gaps = 7/97 (7%)
Frame = +3
Query: 453 RNIAKVAALSSEKDVILARLDDLPSWVFFPDVERAE-------WLNRILLQVWPNVNSYA 611
+ IA A ++ + L D PSWV F ++ WLN L ++WP VN A
Sbjct: 37 KTIAAFARMTVQDSRKLLPGDFYPSWVVFSQRQKLSYSKCLLNWLNLELEKIWPYVNEAA 96
Query: 612 KTLLXDLIEPAVAXSLANYKLSGFKFERMILGTIAPR 722
L+ +EP V L+ KF + LGT+AP+
Sbjct: 97 SELIKSSVEP-VLEQYTPAMLASLKFSKFTLGTVAPQ 132
>At1g05500.1 68414.m00561 C2 domain-containing protein similar to
Ca2+-dependent lipid-binding protein (CLB1) GI:2789434
from [Lycopersicon esculentum]
Length = 528
Score = 38.7 bits (86), Expect = 0.004
Identities = 19/58 (32%), Positives = 30/58 (51%)
Frame = +3
Query: 549 ERAEWLNRILLQVWPNVNSYAKTLLXDLIEPAVAXSLANYKLSGFKFERMILGTIAPR 722
E WLN L ++WP V+ A L+ +EP V ++ F ++ LGT+AP+
Sbjct: 34 ELLTWLNHHLTKIWPYVDEAASELIKASVEP-VLEQYRPAIVASLTFSKLTLGTVAPQ 90
>At5g02390.1 68418.m00162 expressed protein ; expression supported
by MPSS
Length = 835
Score = 29.1 bits (62), Expect = 3.2
Identities = 15/65 (23%), Positives = 31/65 (47%)
Frame = -1
Query: 601 FTFGQTCRSILFNHSARSTSGKNTHEGKSSSLASITSFSEDSAATFAMLRLYSFSLRH*S 422
+ F + R +F H +++ KN ++ + + L IT S + L+LY S + +
Sbjct: 440 YGFSKDLRDDIFEHLKGNSAAKNNNKAEGTKLKQITRASSLCGSLDRYLQLYECSFQREA 499
Query: 421 RITDN 407
+ + N
Sbjct: 500 KNSSN 504
>At5g35210.2 68418.m04175 peptidase M50 family protein /
sterol-regulatory element binding protein (SREBP) site 2
protease family protein contains PFam PF02163:
sterol-regulatory element binding protein (SREBP) site 2
protease
Length = 1409
Score = 28.3 bits (60), Expect = 5.5
Identities = 21/87 (24%), Positives = 40/87 (45%), Gaps = 3/87 (3%)
Frame = +3
Query: 222 CSMASDNNMAGASKTALPPGSDDGNSVFSMMYTFLKKVSIVGAV--YLVGYMQWSVAWLI 395
C + S + A+ + +FS ++ ++ ++ Y++ Y++ S+ LI
Sbjct: 730 CRLTSASRRGCMLNAAVAGATKGAMKIFSGLFPLKNGEGVLSSIAAYIL-YLEESLRGLI 788
Query: 396 -GPVILSVMRDQWRKENEYKRNIAKVA 473
GP + R QWRK+ E NI +A
Sbjct: 789 AGPFLSESPRKQWRKQLE--ENICSIA 813
>At5g35210.1 68418.m04174 peptidase M50 family protein /
sterol-regulatory element binding protein (SREBP) site 2
protease family protein contains PFam PF02163:
sterol-regulatory element binding protein (SREBP) site 2
protease
Length = 1576
Score = 28.3 bits (60), Expect = 5.5
Identities = 21/87 (24%), Positives = 40/87 (45%), Gaps = 3/87 (3%)
Frame = +3
Query: 222 CSMASDNNMAGASKTALPPGSDDGNSVFSMMYTFLKKVSIVGAV--YLVGYMQWSVAWLI 395
C + S + A+ + +FS ++ ++ ++ Y++ Y++ S+ LI
Sbjct: 730 CRLTSASRRGCMLNAAVAGATKGAMKIFSGLFPLKNGEGVLSSIAAYIL-YLEESLRGLI 788
Query: 396 -GPVILSVMRDQWRKENEYKRNIAKVA 473
GP + R QWRK+ E NI +A
Sbjct: 789 AGPFLSESPRKQWRKQLE--ENICSIA 813
>At5g28860.1 68418.m03551 hypothetical protein
Length = 183
Score = 28.3 bits (60), Expect = 5.5
Identities = 17/63 (26%), Positives = 31/63 (49%), Gaps = 1/63 (1%)
Frame = +3
Query: 408 LSVMRDQWRKENEYKRNI-AKVAALSSEKDVILARLDDLPSWVFFPDVERAEWLNRILLQ 584
L R++ RK + + A+VA + +++ + ARLD+ V ++ +W L
Sbjct: 104 LQYFRNKKRKVDSRPEELEAEVAKIRAKQKKVTARLDEFKQMVKALEILSEDWEKVDLQL 163
Query: 585 VWP 593
VWP
Sbjct: 164 VWP 166
>At1g72840.1 68414.m08425 disease resistance protein (TIR-NBS-LRR
class), putative domain signature TIR-NBS-LRR exists,
suggestive of a disease resistance protein.
Length = 1183
Score = 28.3 bits (60), Expect = 5.5
Identities = 12/25 (48%), Positives = 16/25 (64%)
Frame = +2
Query: 308 YDVHISQKGVDCRSCVLSRLYAMER 382
YDV +S +GVD R ++S LY R
Sbjct: 15 YDVFLSFRGVDTRQTIVSHLYVALR 39
>At1g71691.2 68414.m08276 GDSL-motif lipase/hydrolase family protein
similar to family II lipases EXL3 GI:15054386, EXL1
GI:15054382 from [Arabidopsis thaliana]; contains Pfam
profile: PF00657 Lipase Acylhydrolase with GDSL-like
motif
Length = 384
Score = 28.3 bits (60), Expect = 5.5
Identities = 19/72 (26%), Positives = 31/72 (43%), Gaps = 1/72 (1%)
Frame = +3
Query: 477 LSSEKDVILARLDDLPSWVFFPDVERAEWLNRILLQVWPNVNSYAKTLLXDLIEPAVAXS 656
++S+ +A D + +FF + ++LN L+ +P N Y DL+
Sbjct: 166 VASKSGGAVAIADSVTRSLFFIGMGSNDYLNNYLMPNFPTRNQYNSQQFGDLLVQHYTDQ 225
Query: 657 LAN-YKLSGFKF 689
L Y L G KF
Sbjct: 226 LTRLYNLGGRKF 237
>At1g71691.1 68414.m08275 GDSL-motif lipase/hydrolase family protein
similar to family II lipases EXL3 GI:15054386, EXL1
GI:15054382 from [Arabidopsis thaliana]; contains Pfam
profile: PF00657 Lipase Acylhydrolase with GDSL-like
motif
Length = 283
Score = 28.3 bits (60), Expect = 5.5
Identities = 19/72 (26%), Positives = 31/72 (43%), Gaps = 1/72 (1%)
Frame = +3
Query: 477 LSSEKDVILARLDDLPSWVFFPDVERAEWLNRILLQVWPNVNSYAKTLLXDLIEPAVAXS 656
++S+ +A D + +FF + ++LN L+ +P N Y DL+
Sbjct: 65 VASKSGGAVAIADSVTRSLFFIGMGSNDYLNNYLMPNFPTRNQYNSQQFGDLLVQHYTDQ 124
Query: 657 LAN-YKLSGFKF 689
L Y L G KF
Sbjct: 125 LTRLYNLGGRKF 136
>At5g36930.1 68418.m04427 disease resistance protein (TIR-NBS-LRR
class), putative domain signature TIR-NBS-LRR exists,
suggestive of a disease resistance protein.
Length = 1188
Score = 27.9 bits (59), Expect = 7.3
Identities = 13/27 (48%), Positives = 17/27 (62%), Gaps = 1/27 (3%)
Frame = +2
Query: 308 YDVHISQKGVDCRSCVLSRLY-AMERC 385
YDV +S +G D R LS LY ++ RC
Sbjct: 14 YDVFVSFRGADVRKNFLSHLYDSLRRC 40
>At5g03280.1 68418.m00277 ethylene-insensitive 2 (EIN2) identical to
EIN2 [Arabidopsis thaliana] gi|5231113|gb|AAD41076;
member of the natural resistance-associated macrophage
protein (NRAMP) metal transporter family, PMID:11500563;
metal transport capacity has not been shown,
PMID:11500563, PMID:1038174
Length = 1294
Score = 27.9 bits (59), Expect = 7.3
Identities = 13/44 (29%), Positives = 23/44 (52%)
Frame = -2
Query: 540 GRIPMKASRQVSLVSHLFQKTVQRPLRCCVCIRSPCAIDRASPT 409
G+ +R ++ F K ++ P+ C C++ P + RASPT
Sbjct: 1159 GKYTYVLNRLQGVIDPAFSK-LRTPMTPCFCLQIPASHQRASPT 1201
>At3g61030.1 68416.m06828 C2 domain-containing protein similar to
CLB1 [Lycopersicon esculentum] GI:2789434; contains Pfam
profile PF00168: C2 domain
Length = 592
Score = 27.9 bits (59), Expect = 7.3
Identities = 14/52 (26%), Positives = 29/52 (55%), Gaps = 3/52 (5%)
Frame = +3
Query: 558 EWLNRILLQVWPNVNSYAKTLLXDLIEPAVAXSLANYK---LSGFKFERMIL 704
+WLN++L ++WP + A ++ +EP L +Y+ ++ KF ++ L
Sbjct: 40 KWLNKLLSKMWPYIAEAATMVVRYSVEPL----LEDYRPPGITSLKFSKLTL 87
>At3g60950.1 68416.m06819 C2 domain-containing protein similar to
CLB1 [Lycopersicon esculentum] GI:2789434; contains Pfam
profile PF00168: C2 domain
Length = 592
Score = 27.9 bits (59), Expect = 7.3
Identities = 14/52 (26%), Positives = 29/52 (55%), Gaps = 3/52 (5%)
Frame = +3
Query: 558 EWLNRILLQVWPNVNSYAKTLLXDLIEPAVAXSLANYK---LSGFKFERMIL 704
+WLN++L ++WP + A ++ +EP L +Y+ ++ KF ++ L
Sbjct: 40 KWLNKLLSKMWPYIAEAATMVVRYSVEPL----LEDYRPPGITSLKFSKLTL 87
>At1g69860.1 68414.m08040 proton-dependent oligopeptide transport
(POT) family protein contains Pfam profile: PF00854 POT
family
Length = 555
Score = 27.5 bits (58), Expect = 9.6
Identities = 17/47 (36%), Positives = 27/47 (57%)
Frame = +3
Query: 279 GSDDGNSVFSMMYTFLKKVSIVGAVYLVGYMQWSVAWLIGPVILSVM 419
G + S FS YT V +V ++ LV Y+Q +++W IG I +V+
Sbjct: 174 GREGSRSFFSWYYTTHTIVQLV-SMTLVLYVQNNISWGIGFAIPTVL 219
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 15,278,975
Number of Sequences: 28952
Number of extensions: 306661
Number of successful extensions: 793
Number of sequences better than 10.0: 18
Number of HSP's better than 10.0 without gapping: 768
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 792
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1594686376
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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