BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= brP-0182 (800 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC26A3.05 |chc1||clathrin heavy chain Chc1 |Schizosaccharomyce... 264 1e-71 SPCC613.04c |rng3||UCS-domain protein |Schizosaccharomyces pombe... 31 0.19 SPAC9.13c |cwf16|SPAPJ735.01c|splicing factor |Schizosaccharomyc... 28 1.3 SPAPB15E9.01c ||SPAPB18E9.06c|sequence orphan|Schizosaccharomyce... 27 4.1 SPCC576.10c |rpt3||19S proteasome regulatory subunit Rpt3|Schizo... 26 5.4 SPBC20F10.07 |||GRAM domain protein|Schizosaccharomyces pombe|ch... 26 5.4 SPAC16A10.03c |||zinc finger protein Pep5/Vps11 |Schizosaccharom... 26 5.4 SPBC28F2.04c |cwf7|spf27|splicing factor Cwf7|Schizosaccharomyce... 26 7.2 SPAC22F3.03c |rdh54|tid1, mug34|ATP-dependent DNA helicase Rdh54... 26 7.2 SPAC1006.07 |||translation initiation factor eIF4A|Schizosacchar... 25 9.5 >SPAC26A3.05 |chc1||clathrin heavy chain Chc1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 1666 Score = 264 bits (647), Expect = 1e-71 Identities = 128/252 (50%), Positives = 177/252 (70%), Gaps = 20/252 (7%) Frame = +3 Query: 45 WDDLVRYLQMARKKARESYIESELIYAYARTGRLADLEEFISGPNHADIQKIGDRCFDDK 224 +++L++YL MAR K E ++S L+ AYA+T +L ++E F+ G N AD++ +GD CF+ K Sbjct: 1146 YEELIKYLLMARSKMHEPDVDSALLIAYAKTNQLTEMETFLIGSNVADVKAVGDECFESK 1205 Query: 225 MYNAAKLLYNNVSNFARLAITLVHLKEFQGAVDAG--------------------EFRLS 344 Y AAKL+Y+++SN++ LA TLV+L E+QGAVD EFRL+ Sbjct: 1206 NYEAAKLMYSSISNWSMLATTLVYLGEYQGAVDCARKANSIKVWKQVGTACIDKREFRLA 1265 Query: 345 QMCGLHIVVHADELEDLINYYQDRGHFDELISLLEAALGLERAHMGMFTELAILYSKYKP 524 Q+CGL+++VHA+EL LI Y++RG+F+E+ISL+EA LGLERAHM +TELAILY+KYKP Sbjct: 1266 QICGLNLIVHAEELPGLIRLYEERGYFEEVISLMEAGLGLERAHMAFYTELAILYAKYKP 1325 Query: 525 VKMXEHLELFWSRVNIPXVLRAAEHAHLWSELVFLYDKYXEYDNAALTMMQHPTEAWREG 704 +M EHL+LFW R+N+ V+RA + HLW+E VFLY YDNAA MM+ P EA+ Sbjct: 1326 ERMMEHLKLFWGRLNMAKVIRACDQMHLWNEAVFLYVHDQSYDNAAAVMMEQP-EAFDHQ 1384 Query: 705 HFKDIITKVANM 740 FKDII VAN+ Sbjct: 1385 SFKDIIVHVANL 1396 >SPCC613.04c |rng3||UCS-domain protein |Schizosaccharomyces pombe|chr 3|||Manual Length = 746 Score = 31.1 bits (67), Expect = 0.19 Identities = 25/82 (30%), Positives = 42/82 (51%), Gaps = 2/82 (2%) Frame = +3 Query: 72 MARKKARESYIESEL--IYAYARTGRLADLEEFISGPNHADIQKIGDRCFDDKMYNAAKL 245 M + K +S E EL + A + D +E + P IQKI + K+++AAK Sbjct: 399 MCKFKITKSEHERELNQLRNMAEASKTIDYKEDETAPTERRIQKILEYDILSKLFSAAK- 457 Query: 246 LYNNVSNFARLAITLVHLKEFQ 311 YN+++ LA+ LVH+ ++ Sbjct: 458 HYNSLNGL--LAMILVHMANYK 477 >SPAC9.13c |cwf16|SPAPJ735.01c|splicing factor |Schizosaccharomyces pombe|chr 1|||Manual Length = 270 Score = 28.3 bits (60), Expect = 1.3 Identities = 13/37 (35%), Positives = 19/37 (51%) Frame = +3 Query: 78 RKKARESYIESELIYAYARTGRLADLEEFISGPNHAD 188 ++K E Y +++ Y R R A FI+ P HAD Sbjct: 65 KEKTGEKYFSIDILRFYIRCTRCAAEITFITDPKHAD 101 >SPAPB15E9.01c ||SPAPB18E9.06c|sequence orphan|Schizosaccharomyces pombe|chr 1|||Manual Length = 1036 Score = 26.6 bits (56), Expect = 4.1 Identities = 17/51 (33%), Positives = 27/51 (52%) Frame = +1 Query: 118 STLTPAPVASPI*KSLSLARIMLTSRKSVTVVSTIRCTTLRNSCITTSATS 270 S++T + +AS S SLA S S T S+ ++L ++ + SATS Sbjct: 142 SSITSSSLASSSTTSSSLASSSTNSTTSATPTSSATSSSLSSTAASNSATS 192 >SPCC576.10c |rpt3||19S proteasome regulatory subunit Rpt3|Schizosaccharomyces pombe|chr 3|||Manual Length = 389 Score = 26.2 bits (55), Expect = 5.4 Identities = 11/31 (35%), Positives = 21/31 (67%) Frame = +3 Query: 72 MARKKARESYIESELIYAYARTGRLADLEEF 164 +A +K+R ++S++ AYAR + D+E+F Sbjct: 355 LAVRKSRGVILQSDIEEAYARAVKPTDMEQF 385 >SPBC20F10.07 |||GRAM domain protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 764 Score = 26.2 bits (55), Expect = 5.4 Identities = 12/36 (33%), Positives = 18/36 (50%) Frame = +3 Query: 375 ADELEDLINYYQDRGHFDELISLLEAALGLERAHMG 482 ADE E+ Y ++ H + + E + LER H G Sbjct: 104 ADEAENEETSYSEQNHTENTEEIAEESRPLERTHSG 139 >SPAC16A10.03c |||zinc finger protein Pep5/Vps11 |Schizosaccharomyces pombe|chr 1|||Manual Length = 860 Score = 26.2 bits (55), Expect = 5.4 Identities = 15/42 (35%), Positives = 24/42 (57%), Gaps = 1/42 (2%) Frame = +3 Query: 51 DLVRYLQ-MARKKARESYIESELIYAYARTGRLADLEEFISG 173 +L YL+ ++ K S+ S LIY Y + +L L E++SG Sbjct: 424 ELTSYLEALSAKGFAFSHEISTLIYLYIKLRKLDKLTEYVSG 465 >SPBC28F2.04c |cwf7|spf27|splicing factor Cwf7|Schizosaccharomyces pombe|chr 2|||Manual Length = 187 Score = 25.8 bits (54), Expect = 7.2 Identities = 9/29 (31%), Positives = 19/29 (65%) Frame = +3 Query: 51 DLVRYLQMARKKARESYIESELIYAYART 137 D+ RY+Q+ RES ++S +++ Y ++ Sbjct: 76 DVQRYIQLKPPGTRESLLQSAVVWEYQKS 104 >SPAC22F3.03c |rdh54|tid1, mug34|ATP-dependent DNA helicase Rdh54|Schizosaccharomyces pombe|chr 1|||Manual Length = 811 Score = 25.8 bits (54), Expect = 7.2 Identities = 23/83 (27%), Positives = 45/83 (54%), Gaps = 4/83 (4%) Frame = +3 Query: 336 RLSQMCGLHIVVHADELEDLINYYQDRGHFDELISLLEAA-LGLERAHMGMF---TELAI 503 RLS++C I++ ++ L QD+ F++ LL ++ L + A + F + A+ Sbjct: 493 RLSKICNSTILLRNEKENFLSTELQDKHVFEQENMLLSSSKLQILAALLKSFQRGCQKAV 552 Query: 504 LYSKYKPVKMXEHLELFWSRVNI 572 + S+YK + E +ELF S +++ Sbjct: 553 IVSQYK--ETLELIELFLSILHV 573 >SPAC1006.07 |||translation initiation factor eIF4A|Schizosaccharomyces pombe|chr 1|||Manual Length = 392 Score = 25.4 bits (53), Expect = 9.5 Identities = 11/30 (36%), Positives = 19/30 (63%) Frame = +3 Query: 282 ITLVHLKEFQGAVDAGEFRLSQMCGLHIVV 371 +TL +K+F AV+ E++L +C L+ V Sbjct: 227 LTLEGIKQFYVAVEKEEWKLDTLCDLYETV 256 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,934,949 Number of Sequences: 5004 Number of extensions: 55948 Number of successful extensions: 139 Number of sequences better than 10.0: 10 Number of HSP's better than 10.0 without gapping: 131 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 138 length of database: 2,362,478 effective HSP length: 72 effective length of database: 2,002,190 effective search space used: 388424860 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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