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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= brP-0179
         (484 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At1g23290.1 68414.m02913 60S ribosomal protein L27A (RPL27aB) si...   114   3e-26
At1g70600.1 68414.m08133 60S ribosomal protein L27A (RPL27aC) id...   113   8e-26
At1g12960.1 68414.m01505 60S ribosomal protein L27A (RPL27aA) si...    44   5e-05
At2g16100.1 68415.m01846 hypothetical protein                          29   2.2  
At4g29090.1 68417.m04163 reverse transcriptase, putative / RNA-d...    27   5.0  

>At1g23290.1 68414.m02913 60S ribosomal protein L27A (RPL27aB)
           similar to 60S RIBOSOMAL PROTEIN L27A GB:P49637
           GI:1710530 from [Arabidopsis thaliana]
          Length = 146

 Score =  114 bits (274), Expect = 3e-26
 Identities = 62/146 (42%), Positives = 78/146 (53%), Gaps = 1/146 (0%)
 Frame = +3

Query: 9   AKNKTRKLRGHVSXXXXXXXXXXXXXXXXXNAGGEHHHRINMDKYHPGYFGKLGMRNFHF 188
           A  K RK RGHVS                 NAGG HHHRI  DKYHPGYFGK+GMR FH 
Sbjct: 4   ALKKNRKKRGHVSAGHGRIGKHRKHPGGRGNAGGMHHHRILFDKYHPGYFGKVGMRYFHK 63

Query: 189 RKNKNFCPVLNLDKLWTLVSEQTRLKYASAPDGKVPVINIVKAXXXXXXXXXXXPK-QPV 365
            +NK FCP++NLDKLW+LV E  + K  S+ D  VP+I++ +            P+ +P 
Sbjct: 64  LRNKFFCPIVNLDKLWSLVPEDVKAK--SSKD-NVPLIDVTQHGFFKVLGKGHLPENKPF 120

Query: 366 IVXXXXXXXXXXXXXXDVGGACVLSA 443
           +V              + GGA VL+A
Sbjct: 121 VVKAKLISKTAEKKIKEAGGAVVLTA 146


>At1g70600.1 68414.m08133 60S ribosomal protein L27A (RPL27aC)
           identical to 60S ribosomal protein L27A GB:P49637
           [Arabidopsis thaliana]
          Length = 146

 Score =  113 bits (271), Expect = 8e-26
 Identities = 61/143 (42%), Positives = 76/143 (53%), Gaps = 1/143 (0%)
 Frame = +3

Query: 18  KTRKLRGHVSXXXXXXXXXXXXXXXXXNAGGEHHHRINMDKYHPGYFGKLGMRNFHFRKN 197
           K RK RGHVS                 NAGG HHHRI  DKYHPGYFGK+GMR FH  +N
Sbjct: 7   KNRKKRGHVSAGHGRIGKHRKHPGGRGNAGGMHHHRILFDKYHPGYFGKVGMRYFHKLRN 66

Query: 198 KNFCPVLNLDKLWTLVSEQTRLKYASAPDGKVPVINIVKAXXXXXXXXXXXPK-QPVIVX 374
           K FCP++NLDKLW+LV E  + K  S  D  VP+I++ +            P+ +P +V 
Sbjct: 67  KFFCPIVNLDKLWSLVPEDVKAK--STKD-NVPLIDVTQHGFFKVLGKGHLPENKPFVVK 123

Query: 375 XXXXXXXXXXXXXDVGGACVLSA 443
                        + GGA VL+A
Sbjct: 124 AKLISKTAEKKIKEAGGAVVLTA 146


>At1g12960.1 68414.m01505 60S ribosomal protein L27A (RPL27aA)
           similar to GB:BAA96068 from [Panax ginseng]
          Length = 104

 Score = 44.0 bits (99), Expect = 5e-05
 Identities = 20/45 (44%), Positives = 28/45 (62%), Gaps = 2/45 (4%)
 Frame = +3

Query: 117 HHRINMDKYHPGYFGK--LGMRNFHFRKNKNFCPVLNLDKLWTLV 245
           H R    +  PG  G   +GMR FH  +NK +C ++NLDKLW++V
Sbjct: 19  HGRFGKHRKLPGSRGNAGVGMRYFHKLRNKFYCQIVNLDKLWSMV 63


>At2g16100.1 68415.m01846 hypothetical protein 
          Length = 250

 Score = 28.7 bits (61), Expect = 2.2
 Identities = 9/19 (47%), Positives = 13/19 (68%)
 Frame = +2

Query: 113 APSQNQHGQVPSWILWQTW 169
           +P  +Q  Q+P WILW+ W
Sbjct: 20  SPRTSQFHQLPLWILWRIW 38


>At4g29090.1 68417.m04163 reverse transcriptase, putative /
           RNA-dependent DNA polymerase, putative similar to
           reverse transcriptase [Arabidopsis thaliana] GI:976278;
           contains Pfam profile PF00075: RNase H
          Length = 575

 Score = 27.5 bits (58), Expect = 5.0
 Identities = 23/77 (29%), Positives = 34/77 (44%), Gaps = 13/77 (16%)
 Frame = +2

Query: 116 PSQNQHGQVPSWILWQTWYEKFPL-*KEQEFLSSFELR*A---LDISL*TDEAEVC---- 271
           P   +  Q+  W+LW+ W  +  L  + +EF +   LR A   L+      EAE C    
Sbjct: 351 PQWEKASQLVPWLLWRLWKNRNELVFRGREFNAQEVLRRAEDDLEEWRIRTEAESCGTKP 410

Query: 272 -----ICSRWQGPRHQY 307
                 C RW+ P HQ+
Sbjct: 411 QVNRSSCGRWRPPPHQW 427


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 9,143,164
Number of Sequences: 28952
Number of extensions: 182948
Number of successful extensions: 468
Number of sequences better than 10.0: 5
Number of HSP's better than 10.0 without gapping: 449
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 467
length of database: 12,070,560
effective HSP length: 76
effective length of database: 9,870,208
effective search space used: 829097472
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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