BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= brP-0177 (773 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC6G10.08 |idp1||isocitrate dehydrogenase Idp1|Schizosaccharom... 292 4e-80 SPBC6B1.05c |||ubiquitin-like conjugating enzyme|Schizosaccharom... 28 1.3 SPAC688.04c |gst3||glutathione S-transferase |Schizosaccharomyce... 27 2.3 SPBC1539.09c |trp1||anthranilate synthase component II|Schizosac... 26 6.9 SPBC83.11 |||triose phosphate transporter|Schizosaccharomyces po... 25 9.1 SPAC1F5.07c |hem14||protoporphyrinogen oxidase|Schizosaccharomyc... 25 9.1 >SPAC6G10.08 |idp1||isocitrate dehydrogenase Idp1|Schizosaccharomyces pombe|chr 1|||Manual Length = 418 Score = 292 bits (716), Expect = 4e-80 Identities = 134/228 (58%), Positives = 171/228 (75%), Gaps = 2/228 (0%) Frame = +2 Query: 92 KRVVAAKPVVEMDGDEMTRIMWAKIKERLIFPYVKVDCLYFDLGLPHRDATDDQVTIDSA 271 +++ PVVEMDGDEMTR++W I+E+L+ PY+ + Y+DLG+ RD T+DQ+T+D+A Sbjct: 12 QKITVKNPVVEMDGDEMTRVIWKIIREKLVLPYMDIKLDYYDLGIEARDKTNDQITVDAA 71 Query: 272 HAILKHNVGIKCATITPDEQRVEEFKLKKMWLSPNGTIRNILGGTVFREPILCQSIPRVV 451 AILK++VGIKCATITPDE RV+E+ LKKMW SPNGTIRNIL GTVFREPIL ++IP+ + Sbjct: 72 KAILKNDVGIKCATITPDEARVKEYNLKKMWKSPNGTIRNILNGTVFREPILIKNIPKYI 131 Query: 452 PGWTKPIVIXRHAHGDQYXAQDFVVPKPGKVELVYTTRDGTT--EXRVLYDFKTPGVAMG 625 PGWT PI I RHA GDQY + D V PGK+EL +T + + E +Y+F GVAM Sbjct: 132 PGWTNPICIGRHAFGDQYKSTDLVASGPGKLELSFTPKGNPSAKETYNVYEFNGSGVAMS 191 Query: 626 MYNTDESIRSFXHXSFXVALQKKWXLYLSTXNTILKRYHGSXQXHFRK 769 MYNTD+SIR F H SF +ALQKK LYLST NTILK+Y G + F++ Sbjct: 192 MYNTDDSIRGFAHSSFQMALQKKMPLYLSTKNTILKKYDGRFKDTFQE 239 >SPBC6B1.05c |||ubiquitin-like conjugating enzyme|Schizosaccharomyces pombe|chr 2|||Manual Length = 649 Score = 28.3 bits (60), Expect = 1.3 Identities = 20/56 (35%), Positives = 26/56 (46%), Gaps = 2/56 (3%) Frame = +3 Query: 417 NRYCVRVSRGL--CLVGRNLL*XAVMRTATNTXRKILLYRNPERLNLFTLHEMVRQ 578 N C+ + G C V RNLL V R T + Y NP R +LFT + R+ Sbjct: 335 NSKCLLLGAGTLGCGVARNLLSWGV-RHVTFVDYSTVSYSNPVRQSLFTFEDCKRK 389 >SPAC688.04c |gst3||glutathione S-transferase |Schizosaccharomyces pombe|chr 1|||Manual Length = 242 Score = 27.5 bits (58), Expect = 2.3 Identities = 14/41 (34%), Positives = 22/41 (53%) Frame = +2 Query: 245 DDQVTIDSAHAILKHNVGIKCATITPDEQRVEEFKLKKMWL 367 DD VT + AIL+H V + P E+ V E + ++W+ Sbjct: 56 DDGVTYIESAAILEHLVRKYGPSFKPSEEDVAELEKYELWM 96 >SPBC1539.09c |trp1||anthranilate synthase component II|Schizosaccharomyces pombe|chr 2|||Manual Length = 759 Score = 25.8 bits (54), Expect = 6.9 Identities = 17/57 (29%), Positives = 29/57 (50%) Frame = +2 Query: 8 RNMANTSTTKILKCVNQFASLTRNYGTAKRVVAAKPVVEMDGDEMTRIMWAKIKERL 178 R + N S+++I L + GT + ++AAK +VE GD + I K K ++ Sbjct: 516 RELLNLSSSEISNGKKTSTPLVKVCGT-RSLLAAKTIVESGGDLIGLIFVEKSKRKV 571 >SPBC83.11 |||triose phosphate transporter|Schizosaccharomyces pombe|chr 2|||Manual Length = 434 Score = 25.4 bits (53), Expect = 9.1 Identities = 15/41 (36%), Positives = 23/41 (56%) Frame = -3 Query: 399 PKMLRIVPFGLSHIFFNLNSSTLCSSGVIVAHLMPTLCFRM 277 P L ++ GLSH F N+ + TL S VA+ + +L R+ Sbjct: 223 PVFLNLIYNGLSHFFQNILAFTLLSIISPVAYSIASLIKRI 263 >SPAC1F5.07c |hem14||protoporphyrinogen oxidase|Schizosaccharomyces pombe|chr 1|||Manual Length = 490 Score = 25.4 bits (53), Expect = 9.1 Identities = 12/21 (57%), Positives = 15/21 (71%) Frame = -3 Query: 189 YGNISLSLILAHMMRVISSPS 127 YGNI+L LI A + R I SP+ Sbjct: 201 YGNITLGLIRALLAREILSPA 221 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 3,084,839 Number of Sequences: 5004 Number of extensions: 61013 Number of successful extensions: 136 Number of sequences better than 10.0: 6 Number of HSP's better than 10.0 without gapping: 127 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 135 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 373338084 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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