BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= brP-0177
(773 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC6G10.08 |idp1||isocitrate dehydrogenase Idp1|Schizosaccharom... 292 4e-80
SPBC6B1.05c |||ubiquitin-like conjugating enzyme|Schizosaccharom... 28 1.3
SPAC688.04c |gst3||glutathione S-transferase |Schizosaccharomyce... 27 2.3
SPBC1539.09c |trp1||anthranilate synthase component II|Schizosac... 26 6.9
SPBC83.11 |||triose phosphate transporter|Schizosaccharomyces po... 25 9.1
SPAC1F5.07c |hem14||protoporphyrinogen oxidase|Schizosaccharomyc... 25 9.1
>SPAC6G10.08 |idp1||isocitrate dehydrogenase
Idp1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 418
Score = 292 bits (716), Expect = 4e-80
Identities = 134/228 (58%), Positives = 171/228 (75%), Gaps = 2/228 (0%)
Frame = +2
Query: 92 KRVVAAKPVVEMDGDEMTRIMWAKIKERLIFPYVKVDCLYFDLGLPHRDATDDQVTIDSA 271
+++ PVVEMDGDEMTR++W I+E+L+ PY+ + Y+DLG+ RD T+DQ+T+D+A
Sbjct: 12 QKITVKNPVVEMDGDEMTRVIWKIIREKLVLPYMDIKLDYYDLGIEARDKTNDQITVDAA 71
Query: 272 HAILKHNVGIKCATITPDEQRVEEFKLKKMWLSPNGTIRNILGGTVFREPILCQSIPRVV 451
AILK++VGIKCATITPDE RV+E+ LKKMW SPNGTIRNIL GTVFREPIL ++IP+ +
Sbjct: 72 KAILKNDVGIKCATITPDEARVKEYNLKKMWKSPNGTIRNILNGTVFREPILIKNIPKYI 131
Query: 452 PGWTKPIVIXRHAHGDQYXAQDFVVPKPGKVELVYTTRDGTT--EXRVLYDFKTPGVAMG 625
PGWT PI I RHA GDQY + D V PGK+EL +T + + E +Y+F GVAM
Sbjct: 132 PGWTNPICIGRHAFGDQYKSTDLVASGPGKLELSFTPKGNPSAKETYNVYEFNGSGVAMS 191
Query: 626 MYNTDESIRSFXHXSFXVALQKKWXLYLSTXNTILKRYHGSXQXHFRK 769
MYNTD+SIR F H SF +ALQKK LYLST NTILK+Y G + F++
Sbjct: 192 MYNTDDSIRGFAHSSFQMALQKKMPLYLSTKNTILKKYDGRFKDTFQE 239
>SPBC6B1.05c |||ubiquitin-like conjugating
enzyme|Schizosaccharomyces pombe|chr 2|||Manual
Length = 649
Score = 28.3 bits (60), Expect = 1.3
Identities = 20/56 (35%), Positives = 26/56 (46%), Gaps = 2/56 (3%)
Frame = +3
Query: 417 NRYCVRVSRGL--CLVGRNLL*XAVMRTATNTXRKILLYRNPERLNLFTLHEMVRQ 578
N C+ + G C V RNLL V R T + Y NP R +LFT + R+
Sbjct: 335 NSKCLLLGAGTLGCGVARNLLSWGV-RHVTFVDYSTVSYSNPVRQSLFTFEDCKRK 389
>SPAC688.04c |gst3||glutathione S-transferase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 242
Score = 27.5 bits (58), Expect = 2.3
Identities = 14/41 (34%), Positives = 22/41 (53%)
Frame = +2
Query: 245 DDQVTIDSAHAILKHNVGIKCATITPDEQRVEEFKLKKMWL 367
DD VT + AIL+H V + P E+ V E + ++W+
Sbjct: 56 DDGVTYIESAAILEHLVRKYGPSFKPSEEDVAELEKYELWM 96
>SPBC1539.09c |trp1||anthranilate synthase component
II|Schizosaccharomyces pombe|chr 2|||Manual
Length = 759
Score = 25.8 bits (54), Expect = 6.9
Identities = 17/57 (29%), Positives = 29/57 (50%)
Frame = +2
Query: 8 RNMANTSTTKILKCVNQFASLTRNYGTAKRVVAAKPVVEMDGDEMTRIMWAKIKERL 178
R + N S+++I L + GT + ++AAK +VE GD + I K K ++
Sbjct: 516 RELLNLSSSEISNGKKTSTPLVKVCGT-RSLLAAKTIVESGGDLIGLIFVEKSKRKV 571
>SPBC83.11 |||triose phosphate transporter|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 434
Score = 25.4 bits (53), Expect = 9.1
Identities = 15/41 (36%), Positives = 23/41 (56%)
Frame = -3
Query: 399 PKMLRIVPFGLSHIFFNLNSSTLCSSGVIVAHLMPTLCFRM 277
P L ++ GLSH F N+ + TL S VA+ + +L R+
Sbjct: 223 PVFLNLIYNGLSHFFQNILAFTLLSIISPVAYSIASLIKRI 263
>SPAC1F5.07c |hem14||protoporphyrinogen oxidase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 490
Score = 25.4 bits (53), Expect = 9.1
Identities = 12/21 (57%), Positives = 15/21 (71%)
Frame = -3
Query: 189 YGNISLSLILAHMMRVISSPS 127
YGNI+L LI A + R I SP+
Sbjct: 201 YGNITLGLIRALLAREILSPA 221
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,084,839
Number of Sequences: 5004
Number of extensions: 61013
Number of successful extensions: 136
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 127
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 135
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 373338084
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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