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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= brP-0177
         (773 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At5g14590.1 68418.m01711 isocitrate dehydrogenase, putative / NA...   310   5e-85
At1g65930.1 68414.m07481 isocitrate dehydrogenase, putative / NA...   307   6e-84
At1g54340.1 68414.m06195 isocitrate dehydrogenase, putative / NA...   301   3e-82
At2g33200.1 68415.m04067 F-box family protein contains F-box dom...    28   6.0  
At5g28740.1 68418.m03530 transcription-coupled DNA repair protei...    28   7.9  

>At5g14590.1 68418.m01711 isocitrate dehydrogenase, putative / NADP+
           isocitrate dehydrogenase, putative strong similarity to
           isocitrate dehydrogenase (NADP+) [Nicotiana tabacum]
           GI:3021512; contains Pfam domain PF00180: dehydrogenase,
           isocitrate/isopropylmalate family
          Length = 485

 Score =  310 bits (762), Expect = 5e-85
 Identities = 142/230 (61%), Positives = 175/230 (76%), Gaps = 1/230 (0%)
 Frame = +2

Query: 83  GTAKRVVAAKPVVEMDGDEMTRIMWAKIKERLIFPYVKVDCLYFDLGLPHRDATDDQVTI 262
           G + R+    P+VEMDGDEMTR++W+ IKE+LI PY+ +D  YFDLG+ +RDATDD+VT+
Sbjct: 71  GGSDRIQVQNPIVEMDGDEMTRVIWSMIKEKLILPYLDLDIKYFDLGILNRDATDDKVTV 130

Query: 263 DSAHAILKHNVGIKCATITPDEQRVEEFKLKKMWLSPNGTIRNILGGTVFREPILCQSIP 442
           +SA A LK+NV IKCATITPDE RV+EF LK MW SPNGTIRNIL GTVFREPI+C +IP
Sbjct: 131 ESAEAALKYNVAIKCATITPDEGRVKEFGLKSMWRSPNGTIRNILDGTVFREPIMCSNIP 190

Query: 443 RVVPGWTKPIVIXRHAHGDQYXAQDFVVPKPGKVELVYTTRDGTTEXRV-LYDFKTPGVA 619
           R+VPGW KPI I RHA GDQY A D V+  PGK+++V+   DG     + +YDFK PGVA
Sbjct: 191 RLVPGWEKPICIGRHAFGDQYRATDTVIKGPGKLKMVFVPEDGNAPVELDVYDFKGPGVA 250

Query: 620 MGMYNTDESIRSFXHXSFXVALQKKWXLYLSTXNTILKRYHGSXQXHFRK 769
           + MYN DESIR+F   S  +AL KKW LYLST NTILK+Y G  +  F++
Sbjct: 251 LAMYNVDESIRAFAESSMAMALTKKWPLYLSTKNTILKKYDGRFKDIFQE 300


>At1g65930.1 68414.m07481 isocitrate dehydrogenase, putative / NADP+
           isocitrate dehydrogenase, putative strong similarity to
           isocitrate dehydrogenase SP|Q40345 from [Medicago
           sativa]
          Length = 410

 Score =  307 bits (753), Expect = 6e-84
 Identities = 140/226 (61%), Positives = 172/226 (76%)
 Frame = +2

Query: 92  KRVVAAKPVVEMDGDEMTRIMWAKIKERLIFPYVKVDCLYFDLGLPHRDATDDQVTIDSA 271
           +++  A P+VEMDGDEMTR++W  IK++LI P+V++D  YFDLGLPHRDATDD+VTI+SA
Sbjct: 4   EKIKVANPIVEMDGDEMTRVIWKSIKDKLITPFVELDIKYFDLGLPHRDATDDKVTIESA 63

Query: 272 HAILKHNVGIKCATITPDEQRVEEFKLKKMWLSPNGTIRNILGGTVFREPILCQSIPRVV 451
            A  K+NV IKCATITPDE RV EF LK+MW SPNGTIRNIL GTVFREPI+C+++P++V
Sbjct: 64  EATKKYNVAIKCATITPDEGRVTEFGLKQMWRSPNGTIRNILNGTVFREPIICKNVPKLV 123

Query: 452 PGWTKPIVIXRHAHGDQYXAQDFVVPKPGKVELVYTTRDGTTEXRVLYDFKTPGVAMGMY 631
           PGWTKPI I RHA GDQY A D V+  PGK+ + +  +DG TE  V       GVAM MY
Sbjct: 124 PGWTKPICIGRHAFGDQYRATDAVIKGPGKLTMTFEGKDGKTETEVFTFTGEGGVAMAMY 183

Query: 632 NTDESIRSFXHXSFXVALQKKWXLYLSTXNTILKRYHGSXQXHFRK 769
           NTDESIR+F   S   A +KKW LYLST NTILK+Y G  +  F++
Sbjct: 184 NTDESIRAFADASMNTAYEKKWPLYLSTKNTILKKYDGRFKDIFQE 229


>At1g54340.1 68414.m06195 isocitrate dehydrogenase, putative / NADP+
           isocitrate dehydrogenase, putative strong similarity to
           NADP-isocitrate dehydrogenase GI:5764653 from [Citrus
           limon]; Nicotiana tabacum SP|P50218
          Length = 416

 Score =  301 bits (739), Expect = 3e-82
 Identities = 142/228 (62%), Positives = 175/228 (76%), Gaps = 2/228 (0%)
 Frame = +2

Query: 92  KRVVAAKPVVEMDGDEMTRIMWAKIKERLIFPYVKVDCLYFDLGLPHRDATDDQVTIDSA 271
           +++    PVVEMDGDEMTR++W  IK++LIFP++++D  YFDLGLP+RD TDD+VTI++A
Sbjct: 4   EKIKVINPVVEMDGDEMTRVIWKFIKDKLIFPFLELDIKYFDLGLPNRDFTDDKVTIETA 63

Query: 272 HAILKHNVGIKCATITPDEQRVEEFKLKKMWLSPNGTIRNILGGTVFREPILCQSIPRVV 451
            A LK+NV IKCATITPDE RV EF LKKMW SPNGTIRNIL GTVFREPI+C++IPR+V
Sbjct: 64  EATLKYNVAIKCATITPDEARVREFGLKKMWRSPNGTIRNILNGTVFREPIICRNIPRLV 123

Query: 452 PGWTKPIVIXRHAHGDQYXAQDFVVPKPGKVELVYTTRDGT--TEXRVLYDFKTPGVAMG 625
           PGWTKPI I RHA GDQY A D +V +PGK++LV+     +  TE  V ++F   GVA+ 
Sbjct: 124 PGWTKPICIGRHAFGDQYRATDLIVNEPGKLKLVFEPSGSSQKTEFEV-FNFTGGGVALA 182

Query: 626 MYNTDESIRSFXHXSFXVALQKKWXLYLSTXNTILKRYHGSXQXHFRK 769
           MYNTDESIR+F   S   A QKKW LYLST NTILK Y G  +  F++
Sbjct: 183 MYNTDESIRAFAESSMYTAYQKKWPLYLSTKNTILKIYDGRFKDIFQE 230


>At2g33200.1 68415.m04067 F-box family protein contains F-box domain
           Pfam:PF00646
          Length = 376

 Score = 28.3 bits (60), Expect = 6.0
 Identities = 12/39 (30%), Positives = 22/39 (56%)
 Frame = +1

Query: 349 VEENVAKPERNDP*HFGRYSIPRTDIVSEYPEGCAWLDE 465
           +E ++   ER +     ++ I RTDI+S   + C W++E
Sbjct: 119 MESSILGKERLEKEVAWKHFIERTDIISTKKQACLWINE 157


>At5g28740.1 68418.m03530 transcription-coupled DNA repair
           protein-related similar to XAB2 (XPA-binding protein 2)
           [Homo sapiens] GI:10566459
          Length = 917

 Score = 27.9 bits (59), Expect = 7.9
 Identities = 24/69 (34%), Positives = 33/69 (47%), Gaps = 8/69 (11%)
 Frame = +2

Query: 56  QFASLTRNYGTAKRVV-----AAKPVVEMDGDEMTRIMWAKIKERLIFPYVKVDCLY--- 211
           Q+A L  +YG AKR +     A K V E    EM  I  ++  E  IF   +   +Y   
Sbjct: 629 QYAKLEEDYGLAKRAMKVYEEATKKVPEGQKLEMYEIYISRAAE--IFGVPRTREIYEQA 686

Query: 212 FDLGLPHRD 238
            + GLPH+D
Sbjct: 687 IESGLPHKD 695


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 16,217,110
Number of Sequences: 28952
Number of extensions: 326376
Number of successful extensions: 624
Number of sequences better than 10.0: 5
Number of HSP's better than 10.0 without gapping: 612
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 622
length of database: 12,070,560
effective HSP length: 80
effective length of database: 9,754,400
effective search space used: 1726528800
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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