Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= brP-0176
(775 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 236 3e-63
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 227 1e-60
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 124 2e-29
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 106 3e-24
SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||... 26 6.9
SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr... 26 6.9
SPCC16A11.08 |atg20||sorting nexin Atg20|Schizosaccharomyces pom... 25 9.1
>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 449
Score = 236 bits (577), Expect = 3e-63
Identities = 101/139 (72%), Positives = 119/139 (85%)
Frame = +1
Query: 58 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGK 237
MRE IS+HVGQAG QIGNACWELYCLEHGIQP+G M + D F+TFFSETG GK
Sbjct: 1 MREIISIHVGQAGTQIGNACWELYCLEHGIQPNGYMNPETASQNSDGGFSTFFSETGQGK 60
Query: 238 HVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLD 417
+VPR+++VDLEP V+D+VRTG YR LFHPEQLITGKEDA+NNYARGHYT+GKE+VD V D
Sbjct: 61 YVPRSIYVDLEPNVIDQVRTGPYRDLFHPEQLITGKEDASNNYARGHYTVGKELVDEVTD 120
Query: 418 RIRKLADQCTGLQGFLIFH 474
+IR++AD C+GLQGFL+FH
Sbjct: 121 KIRRIADNCSGLQGFLVFH 139
Score = 146 bits (353), Expect = 4e-36
Identities = 64/85 (75%), Positives = 77/85 (90%)
Frame = +2
Query: 521 ERLSVDYGKKSKLEFAIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICR 700
ERL+++Y KKSKL+F++YPAPQVST+VVEPYNS+LTTH TL+ +DC FMVDNE+ YDICR
Sbjct: 155 ERLAMEYTKKSKLQFSVYPAPQVSTSVVEPYNSVLTTHATLDLADCTFMVDNESCYDICR 214
Query: 701 RNLDIERPTYTNLNRLIGQIXSSIT 775
RNLDIERP+Y NLNRLI Q+ SSIT
Sbjct: 215 RNLDIERPSYENLNRLIAQVVSSIT 239
>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 455
Score = 227 bits (556), Expect = 1e-60
Identities = 102/144 (70%), Positives = 121/144 (84%), Gaps = 5/144 (3%)
Frame = +1
Query: 58 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTD-----KTIGGGDDSFNTFFSE 222
MRE ISVHVGQAGVQIGNACWELYCLEHGI PDG PT+ K +D F TFFSE
Sbjct: 1 MREVISVHVGQAGVQIGNACWELYCLEHGIGPDG-FPTENSEVHKNNSYLNDGFGTFFSE 59
Query: 223 TGAGKHVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIV 402
TG GK VPR+++VDLEP V+D+VRTG Y+ LFHPEQ++TGKEDA+NNYARGHYT+GKE++
Sbjct: 60 TGQGKFVPRSIYVDLEPNVIDQVRTGPYKDLFHPEQMVTGKEDASNNYARGHYTVGKEMI 119
Query: 403 DLVLDRIRKLADQCTGLQGFLIFH 474
D VL+RIR++AD C+GLQGFL+FH
Sbjct: 120 DSVLERIRRMADNCSGLQGFLVFH 143
Score = 153 bits (370), Expect = 4e-38
Identities = 67/85 (78%), Positives = 78/85 (91%)
Frame = +2
Query: 521 ERLSVDYGKKSKLEFAIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICR 700
ERL+++YGKKS L+F++YPAPQVST+VVEPYNS+LTTH TL++SDC FMVDNEA YDICR
Sbjct: 159 ERLNMEYGKKSNLQFSVYPAPQVSTSVVEPYNSVLTTHATLDNSDCTFMVDNEACYDICR 218
Query: 701 RNLDIERPTYTNLNRLIGQIXSSIT 775
RNLDIERPTY NLNRLI Q+ SSIT
Sbjct: 219 RNLDIERPTYENLNRLIAQVVSSIT 243
>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 448
Score = 124 bits (298), Expect = 2e-29
Identities = 59/139 (42%), Positives = 81/139 (58%)
Frame = +1
Query: 58 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGK 237
MRE + + GQ G Q+G A W EHG+ G T + N +F+E GK
Sbjct: 1 MREIVHIQAGQCGNQVGAAFWSTIADEHGLDSAGIY--HGTSEAQHERLNVYFNEAAGGK 58
Query: 238 HVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLD 417
+VPRAV VDLEP +D V++G + LF P+ +I G+ A N +A+GHYT G E+ D VLD
Sbjct: 59 YVPRAVLVDLEPGTMDAVKSGKFGNLFRPDNIIYGQSGAGNIWAKGHYTEGAELADAVLD 118
Query: 418 RIRKLADQCTGLQGFLIFH 474
+R+ A+ C LQGF + H
Sbjct: 119 VVRREAEACDALQGFQLTH 137
Score = 71.7 bits (168), Expect = 1e-13
Identities = 31/84 (36%), Positives = 51/84 (60%)
Frame = +2
Query: 524 RLSVDYGKKSKLEFAIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRR 703
++ +Y + F++ PAP+ S VVEPYN+ L+ H +E+SD F +DNEA+ I
Sbjct: 154 KIREEYPDRMMATFSVAPAPKSSDTVVEPYNATLSMHQLVENSDETFCIDNEALSSIFAN 213
Query: 704 NLDIERPTYTNLNRLIGQIXSSIT 775
L I+ P+Y +LN L+ + + +T
Sbjct: 214 TLKIKSPSYDDLNHLVSAVMAGVT 237
>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 446
Score = 106 bits (255), Expect = 3e-24
Identities = 54/140 (38%), Positives = 84/140 (60%), Gaps = 2/140 (1%)
Frame = +1
Query: 61 RECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGKH 240
RE I++ GQ G QIG+ W+ CLEHGI PDG + + T G D + FF ++ ++
Sbjct: 3 REIITLQAGQCGNQIGSQFWQQLCLEHGIGPDGTLESFAT--EGVDRKDVFFYQSDDTRY 60
Query: 241 VPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKE--DAANNYARGHYTIGKEIVDLVL 414
+PRA+ +DLEP VV+ + + TY L++PE ++ K A NN+A G Y+ + I + ++
Sbjct: 61 IPRAILIDLEPRVVNNILSDTYGSLYNPENILITKNGGGAGNNWANG-YSHAERIFEDIM 119
Query: 415 DRIRKLADQCTGLQGFLIFH 474
D I + AD L+GF + H
Sbjct: 120 DMIDREADGSDSLEGFSLLH 139
Score = 54.4 bits (125), Expect = 2e-08
Identities = 29/86 (33%), Positives = 47/86 (54%), Gaps = 1/86 (1%)
Frame = +2
Query: 521 ERLSVDYGKKSKLEFAIYPAPQ-VSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDIC 697
ERL+ Y KK ++++P Q VS VV+PYNS+L ++D ++DN A+ I
Sbjct: 155 ERLNDRYPKKIIQTYSVFPNSQSVSDVVVQPYNSLLALKRLTLNADSVVVLDNAALAHIA 214
Query: 698 RRNLDIERPTYTNLNRLIGQIXSSIT 775
L + PT+ N+L+ + S+ T
Sbjct: 215 ADRLHTQNPTFHQQNQLVSTVMSAST 240
>SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr
2|||Manual
Length = 534
Score = 25.8 bits (54), Expect = 6.9
Identities = 15/35 (42%), Positives = 19/35 (54%)
Frame = +2
Query: 137 STASSLMARCPQTRPSGVETILSTLSSARPELAST 241
ST SSL + ++PS T ST SSA P S+
Sbjct: 173 STFSSLSSSTSSSQPSVSSTSSSTFSSAAPTSTSS 207
>SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1275
Score = 25.8 bits (54), Expect = 6.9
Identities = 13/22 (59%), Positives = 13/22 (59%)
Frame = +3
Query: 156 WPDAHRQDHRGWRRFFQHFLQR 221
W A R D R R FQHFLQR
Sbjct: 590 WLAACRSDPRCRRLDFQHFLQR 611
>SPCC16A11.08 |atg20||sorting nexin Atg20|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 534
Score = 25.4 bits (53), Expect = 9.1
Identities = 15/41 (36%), Positives = 21/41 (51%)
Frame = +2
Query: 47 LKSKCVSASLYTLAKPESRSVMPAGSFTAWSTASSLMARCP 169
L+S +L+ P SR++ P S + STASSL P
Sbjct: 170 LRSSMPLVMANSLSPPSSRALKPIHSLSNPSTASSLEPSSP 210
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,185,940
Number of Sequences: 5004
Number of extensions: 67122
Number of successful extensions: 239
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 189
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 236
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 373338084
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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