Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= brP-0156
(774 letters)
Database: bee
438 sequences; 146,343 total letters
Searching......................................................done
Score E
Sequences producing significant alignments: (bits) Value
AF015267-1|AAC38959.1| 461|Apis mellifera elongation factor-1al... 492 e-141
X52884-1|CAA37066.1| 461|Apis mellifera elongation factor 1 alp... 476 e-137
AY208278-1|AAO48970.1| 274|Apis mellifera elongation factor 1-a... 382 e-108
EF013389-1|ABK54743.1| 172|Apis mellifera elongation factor 1-a... 328 5e-92
AF069739-1|AAC63272.2| 690|Apis mellifera translation initiatio... 38 1e-04
AM050259-1|CAJ18340.1| 683|Apis mellifera putative H3K9 methylt... 24 1.4
AB253417-1|BAE86928.1| 567|Apis mellifera alpha-glucosidase pro... 23 4.2
D79208-1|BAA11466.1| 567|Apis mellifera alpha-glucosidase protein. 22 5.5
>AF015267-1|AAC38959.1| 461|Apis mellifera elongation factor-1alpha
F2 protein.
Length = 461
Score = 492 bits (1213), Expect = e-141
Identities = 233/255 (91%), Positives = 237/255 (92%)
Frame = +1
Query: 10 RXKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDK 189
+ K HINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDK
Sbjct: 3 KEKIHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDK 62
Query: 190 LKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSXADCAVLIVAAGTGE 369
LKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTS ADCAVLIVAAGTGE
Sbjct: 63 LKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGE 122
Query: 370 FEAGISKNGQTREHALLAFTLGVKQLIVXVNKMDSTEPPYSEPRFEEIKXEVSSYIKKIG 549
FEAGISKNGQTREHALLAFTLGVKQLIV VNKMDSTEPPYSE RFEEIK EVSSYIKKIG
Sbjct: 123 FEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSETRFEEIKKEVSSYIKKIG 182
Query: 550 YNPSAVAFVPISGWHGDNMLEPSTKMPWFXGWXVERXESKADXKCLIEALDAXLAPARPT 729
YNP+AVAFVPISGWHGDNMLE S+KMPWF GW VER E K + KCLIEALDA L P RPT
Sbjct: 183 YNPAAVAFVPISGWHGDNMLEVSSKMPWFKGWTVERKEGKVEGKCLIEALDAILPPTRPT 242
Query: 730 DKPLRLXLQDVYKIG 774
DK LRL LQDVYKIG
Sbjct: 243 DKALRLPLQDVYKIG 257
>X52884-1|CAA37066.1| 461|Apis mellifera elongation factor 1 alpha
protein.
Length = 461
Score = 476 bits (1174), Expect = e-137
Identities = 225/255 (88%), Positives = 233/255 (91%)
Frame = +1
Query: 10 RXKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDK 189
+ K HINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDK
Sbjct: 3 KEKIHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDK 62
Query: 190 LKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSXADCAVLIVAAGTGE 369
LKAERERGITIDIALWKFET+KYYVTIIDAPGHRDFIKNMITGTS ADCAVLIVAAG GE
Sbjct: 63 LKAERERGITIDIALWKFETAKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGIGE 122
Query: 370 FEAGISKNGQTREHALLAFTLGVKQLIVXVNKMDSTEPPYSEPRFEEIKXEVSSYIKKIG 549
FEAGISKNGQTREHALLAFTLGVKQLIV VNKMD T+PPYSE RFEEIK EVSSYIKKIG
Sbjct: 123 FEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDMTDPPYSEARFEEIKKEVSSYIKKIG 182
Query: 550 YNPSAVAFVPISGWHGDNMLEPSTKMPWFXGWXVERXESKADXKCLIEALDAXLAPARPT 729
YN ++VAFVPISGWHGDNMLEPS K PW+ GW VER + AD K LIEALDA L P+RPT
Sbjct: 183 YNTASVAFVPISGWHGDNMLEPSPKTPWYKGWKVERKDGNADGKTLIEALDAILPPSRPT 242
Query: 730 DKPLRLXLQDVYKIG 774
DK LRL LQDVYKIG
Sbjct: 243 DKALRLPLQDVYKIG 257
>AY208278-1|AAO48970.1| 274|Apis mellifera elongation factor
1-alpha protein.
Length = 274
Score = 382 bits (939), Expect = e-108
Identities = 181/200 (90%), Positives = 184/200 (92%)
Frame = +1
Query: 175 WVLDKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSXADCAVLIVA 354
WVLDKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTS ADCAVLIVA
Sbjct: 1 WVLDKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVA 60
Query: 355 AGTGEFEAGISKNGQTREHALLAFTLGVKQLIVXVNKMDSTEPPYSEPRFEEIKXEVSSY 534
AGTGEFEAGISKNGQTREHALLAFTLGVKQLIV VNKMDSTEPPYSE RFEEIK EVSSY
Sbjct: 61 AGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSETRFEEIKKEVSSY 120
Query: 535 IKKIGYNPSAVAFVPISGWHGDNMLEPSTKMPWFXGWXVERXESKADXKCLIEALDAXLA 714
IKKIGYNP+AVAFVPISGWHGDNMLE S+KMPWF GW VER E K + KCLIEALDA L
Sbjct: 121 IKKIGYNPAAVAFVPISGWHGDNMLEVSSKMPWFKGWTVERKEGKVEGKCLIEALDAILP 180
Query: 715 PARPTDKPLRLXLQDVYKIG 774
P RPTDK LRL LQDVYKIG
Sbjct: 181 PTRPTDKALRLPLQDVYKIG 200
>EF013389-1|ABK54743.1| 172|Apis mellifera elongation factor
1-alpha protein.
Length = 172
Score = 328 bits (805), Expect = 5e-92
Identities = 154/171 (90%), Positives = 157/171 (91%)
Frame = +1
Query: 223 DIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSXADCAVLIVAAGTGEFEAGISKNGQT 402
DIALWKFETSKYYVTIIDAPGHRDFIKNMITGTS ADCAVLIVAAGTGEFEAGISKNGQT
Sbjct: 1 DIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQT 60
Query: 403 REHALLAFTLGVKQLIVXVNKMDSTEPPYSEPRFEEIKXEVSSYIKKIGYNPSAVAFVPI 582
REHALLAFTLGVKQLIV VNKMDSTEPPYSE RFEEIK EVSSYIKKIGYNP+AVAFVPI
Sbjct: 61 REHALLAFTLGVKQLIVGVNKMDSTEPPYSETRFEEIKKEVSSYIKKIGYNPAAVAFVPI 120
Query: 583 SGWHGDNMLEPSTKMPWFXGWXVERXESKADXKCLIEALDAXLAPARPTDK 735
SGWHGDNMLE S+KMPWF GW VER E K + KCLIEALDA L P RPTDK
Sbjct: 121 SGWHGDNMLEVSSKMPWFKGWTVERKEGKVEGKCLIEALDAILPPTRPTDK 171
>AF069739-1|AAC63272.2| 690|Apis mellifera translation initiation
factor 2 protein.
Length = 690
Score = 37.9 bits (84), Expect = 1e-04
Identities = 36/117 (30%), Positives = 52/117 (44%)
Frame = +1
Query: 262 VTIIDAPGHRDFIKNMITGTSXADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGVK 441
VT +D PGH FI G D VL+VAA G E QT + +A V
Sbjct: 195 VTFLDTPGHAAFISMRHRGAHITDIVVLVVAADDGVKE-------QTLQSIEMAKDAKV- 246
Query: 442 QLIVXVNKMDSTEPPYSEPRFEEIKXEVSSYIKKIGYNPSAVAFVPISGWHGDNMLE 612
+IV +NK+D + ++E K + I+++G + V IS G N+ E
Sbjct: 247 PIIVAINKIDKPNIDIIKVQYELAKHGI--VIEELG---GEIQCVKISALKGINLRE 298
Score = 25.4 bits (53), Expect = 0.59
Identities = 9/18 (50%), Positives = 13/18 (72%)
Frame = +1
Query: 16 KTHINIVVIGHVDSGKST 69
K H + ++GHVD GK+T
Sbjct: 143 KRHPIVTIMGHVDHGKTT 160
>AM050259-1|CAJ18340.1| 683|Apis mellifera putative H3K9
methyltransferase protein.
Length = 683
Score = 24.2 bits (50), Expect = 1.4
Identities = 11/15 (73%), Positives = 11/15 (73%)
Frame = +1
Query: 25 INIVVIGHVDSGKST 69
INI IGHV GKST
Sbjct: 43 INIGTIGHVAHGKST 57
>AB253417-1|BAE86928.1| 567|Apis mellifera alpha-glucosidase
protein.
Length = 567
Score = 22.6 bits (46), Expect = 4.2
Identities = 13/34 (38%), Positives = 18/34 (52%)
Frame = +1
Query: 247 TSKYYVTIIDAPGHRDFIKNMITGTSXADCAVLI 348
T KYY D P + FIKN+ ++ +D LI
Sbjct: 294 TMKYYDYGADFPFNFAFIKNVSRDSNSSDFKKLI 327
>D79208-1|BAA11466.1| 567|Apis mellifera alpha-glucosidase protein.
Length = 567
Score = 22.2 bits (45), Expect = 5.5
Identities = 12/34 (35%), Positives = 18/34 (52%)
Frame = +1
Query: 247 TSKYYVTIIDAPGHRDFIKNMITGTSXADCAVLI 348
T KYY D P + FIKN+ ++ +D L+
Sbjct: 294 TMKYYDYGADFPFNFAFIKNVSRDSNSSDFKKLV 327
Database: bee
Posted date: Oct 23, 2007 1:17 PM
Number of letters in database: 146,343
Number of sequences in database: 438
Lambda K H
0.318 0.135 0.410
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 204,559
Number of Sequences: 438
Number of extensions: 4183
Number of successful extensions: 17
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 16
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 17
length of database: 146,343
effective HSP length: 57
effective length of database: 121,377
effective search space used: 24275400
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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