Bombyx BLAST Result against arabidopsis

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= brP-0154
         (732 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At2g29500.1 68415.m03583 17.6 kDa class I small heat shock prote...    40   0.002
At1g53540.1 68414.m06074 17.6 kDa class I small heat shock prote...    39   0.003
At1g07400.1 68414.m00789 17.8 kDa class I heat shock protein (HS...    38   0.005
At1g59860.1 68414.m06742 17.6 kDa class I heat shock protein (HS...    38   0.007
At5g59720.1 68418.m07486 18.1 kDa class I heat shock protein (HS...    38   0.009
At4g27670.1 68417.m03979 25.3 kDa small heat shock protein, chlo...    38   0.009
At3g46230.1 68416.m05004 17.4 kDa class I heat shock protein (HS...    37   0.012
At5g55300.1 68418.m06891 DNA topoisomerase I identical to Swiss-...    28   7.3  
At1g31440.1 68414.m03850 SH3 domain-containing protein 1 (SH3P1)...    28   7.3  
At1g35160.1 68414.m04360 14-3-3 protein GF14 phi (GRF4) identica...    27   9.7  

>At2g29500.1 68415.m03583 17.6 kDa class I small heat shock protein
           (HSP17.6B-CI) contains Pfam PF00011: Hsp20/alpha
           crystallin family; identified in Scharf, K-D., et al,
           Cell Stress & Chaperones (2001) 6: 225-237.
          Length = 153

 Score = 39.5 bits (88), Expect = 0.002
 Identities = 33/114 (28%), Positives = 53/114 (46%), Gaps = 8/114 (7%)
 Frame = +3

Query: 201 AIANRDVGSTITXNKDKFQVNLDVQHFSPEEISVKTADGYV--IVEXKHXERQDEHGY-- 368
           AI N  V    T     F+   D+     EE+ V+  +  V  I   +H E++D++    
Sbjct: 42  AIVNARVDWRETPEAHVFKA--DLPGLKKEEVKVEIEEDSVLKISGERHVEKEDKNDTWH 99

Query: 369 ----ISXXFTRRYALPENCNPDTVESRLSSDGVLTVIAPXTPAATKNERAVPIT 518
                S  FTRR+ LPEN   D V++ +  +GVLTV  P       + +++ I+
Sbjct: 100 RVERSSGQFTRRFRLPENVKMDQVKAAM-ENGVLTVTVPKAETKKADVKSIQIS 152


>At1g53540.1 68414.m06074 17.6 kDa class I small heat shock protein
           (HSP17.6C-CI) (AA 1-156) identical to (17.6 kDa class I
           heat shock protein (HSP 17.6) (AA 1-156)(SP:P13853)
           (GI:4376161) (Arabidopsis thaliana) (Nucleic Acids Res.
           17 (19), 7995 (1989))
          Length = 157

 Score = 39.1 bits (87), Expect = 0.003
 Identities = 40/163 (24%), Positives = 70/163 (42%), Gaps = 8/163 (4%)
 Frame = +3

Query: 54  MSLLPFILGYQPNRLRDQDFGLALTPDDLLTAVITPTMSRDYYRPWKQMAIANRDVGSTI 233
           MSL+P I G +   + D     +L   D     +TP+   +        A  N  V    
Sbjct: 1   MSLIPSIFGGRRTNVFDP---FSLDVFDPFEGFLTPSGLANA-PAMDVAAFTNAKVDWRE 56

Query: 234 TXNKDKFQVNLDVQHFSPEEISVKTADGYVIV-----EXKHXERQDEHGYI---SXXFTR 389
           T     F+   D+     EE+ V+  DG ++        ++ E+ D+   +   S  FTR
Sbjct: 57  TPEAHVFKA--DLPGLRKEEVKVEVEDGNILQISGERSNENEEKNDKWHRVERSSGKFTR 114

Query: 390 RYALPENCNPDTVESRLSSDGVLTVIAPXTPAATKNERAVPIT 518
           R+ LPEN   + +++ +  +GVL+V  P  P      +++ I+
Sbjct: 115 RFRLPENAKMEEIKASM-ENGVLSVTVPKVPEKKPEVKSIDIS 156


>At1g07400.1 68414.m00789 17.8 kDa class I heat shock protein
           (HSP17.8-CI) similar to 17.5 kDa class I heat shock
           protein SP:P04793 from [Glycine max]; contains Pfam
           PF00011: Hsp20/alpha crystallin family
          Length = 157

 Score = 38.3 bits (85), Expect = 0.005
 Identities = 34/117 (29%), Positives = 52/117 (44%), Gaps = 8/117 (6%)
 Frame = +3

Query: 201 AIANRDVGSTITXNKDKFQVNLDVQHFSPEEISVKTADGYV--IVEXKHXERQDEHGY-- 368
           AI N  V    T     F+   D+     EE+ V+  D  V  I   +H E++++     
Sbjct: 44  AITNARVDWKETAEAHVFKA--DLPGMKKEEVKVEIEDDSVLKISGERHVEKEEKQDTWH 101

Query: 369 ----ISXXFTRRYALPENCNPDTVESRLSSDGVLTVIAPXTPAATKNERAVPITQTG 527
                S  F+R++ LPEN   D V++ +  +GVLTV  P    A K  +   I  +G
Sbjct: 102 RVERSSGQFSRKFKLPENVKMDQVKASM-ENGVLTVTVPKVEEAKKKAQVKSIDISG 157


>At1g59860.1 68414.m06742 17.6 kDa class I heat shock protein
           (HSP17.6A-CI) similar to 17.5 kDa class I heat shock
           protein SP:P04793 from [Glycine max]
          Length = 155

 Score = 37.9 bits (84), Expect = 0.007
 Identities = 34/117 (29%), Positives = 52/117 (44%), Gaps = 8/117 (6%)
 Frame = +3

Query: 201 AIANRDVGSTITXNKDKFQVNLDVQHFSPEEISVKTADGYV--IVEXKHXERQDEHGY-- 368
           AIAN  V    T     F+   D+     EE+ V+  D  V  I   +H E++++     
Sbjct: 42  AIANARVDWKETAEAHVFKA--DLPGMKKEEVKVEIEDDSVLKISGERHVEKEEKQDTWH 99

Query: 369 ----ISXXFTRRYALPENCNPDTVESRLSSDGVLTVIAPXTPAATKNERAVPITQTG 527
                S  F+R++ LPEN   D V++ +  +GVLTV  P      K  +   I  +G
Sbjct: 100 RVERSSGGFSRKFRLPENVKMDQVKASM-ENGVLTVTVPKVETNKKKAQVKSIDISG 155


>At5g59720.1 68418.m07486 18.1 kDa class I heat shock protein
           (HSP18.1-CI) identical to 18.2 kDa class I heat shock
           protein (HSP 18.2) (SP:P19037)[Arabidopsis thaliana];
           contains Pfam profile: PF00011 Hsp20/alpha crystallin
           family
          Length = 161

 Score = 37.5 bits (83), Expect = 0.009
 Identities = 25/92 (27%), Positives = 45/92 (48%), Gaps = 8/92 (8%)
 Frame = +3

Query: 267 DVQHFSPEEISVKTADGYVIV-----EXKHXERQDEHGYI---SXXFTRRYALPENCNPD 422
           D+     EE+ V+  D  V+        ++ E+ D+   +   S  F RR+ LPEN   +
Sbjct: 68  DLPGLKKEEVKVEVEDKNVLQISGERSKENEEKNDKWHRVERASGKFMRRFRLPENAKME 127

Query: 423 TVESRLSSDGVLTVIAPXTPAATKNERAVPIT 518
            V++ +  +GVLTV+ P  P      +++ I+
Sbjct: 128 EVKATM-ENGVLTVVVPKAPEKKPQVKSIDIS 158


>At4g27670.1 68417.m03979 25.3 kDa small heat shock protein,
           chloroplast precursor (HSP25.3-P) identical to small
           heat shock protein, chloroplast precursor SP:P31170 from
           [Arabidopsis thaliana]; identified in Scharf, K-D., et
           al,Cell Stress & Chaperones (2001) 6: 225-237.
          Length = 227

 Score = 37.5 bits (83), Expect = 0.009
 Identities = 22/91 (24%), Positives = 41/91 (45%), Gaps = 3/91 (3%)
 Frame = +3

Query: 231 ITXNKDKFQVNLDVQHFSPEEISVKTADGYVIV--EXKHXERQDE-HGYISXXFTRRYAL 401
           I   + + ++  D+   S E++ +   D  +++  E K  +  D   G     +  R  L
Sbjct: 131 IKEEEHEIKMRFDMPGLSKEDVKISVEDNVLVIKGEQKKEDSDDSWSGRSVSSYGTRLQL 190

Query: 402 PENCNPDTVESRLSSDGVLTVIAPXTPAATK 494
           P+NC  D +++ L  +GVL +  P T    K
Sbjct: 191 PDNCEKDKIKAEL-KNGVLFITIPKTKVERK 220


>At3g46230.1 68416.m05004 17.4 kDa class I heat shock protein
           (HSP17.4-CI) identical to 17.4 kDa class I heat shock
           protein SP:P19036 from [Arabidopsis thaliana]
          Length = 156

 Score = 37.1 bits (82), Expect = 0.012
 Identities = 42/164 (25%), Positives = 70/164 (42%), Gaps = 9/164 (5%)
 Frame = +3

Query: 54  MSLLPFILGYQPNRLRDQDFGLALTPDDLLTAVITPTMSRDYYRPWKQMA-IANRDVGST 230
           MSL+P   G +   + D     +L   D     +TP ++     P K +A   N  V   
Sbjct: 1   MSLVPSFFGGRRTNVFDP---FSLDVWDPFEGFLTPGLTN---APAKDVAAFTNAKVDWR 54

Query: 231 ITXNKDKFQVNLDVQHFSPEEISVKTADGYVIV-----EXKHXERQDEHGYI---SXXFT 386
            T     F+   DV     EE+ V+  DG ++        ++ E+ D    +   S  F 
Sbjct: 55  ETPEAHVFKA--DVPGLKKEEVKVEVEDGNILQISGERSSENEEKSDTWHRVERSSGKFM 112

Query: 387 RRYALPENCNPDTVESRLSSDGVLTVIAPXTPAATKNERAVPIT 518
           RR+ LPEN   + V++ +  +GVL+V  P    +    ++V I+
Sbjct: 113 RRFRLPENAKVEEVKASM-ENGVLSVTVPKVQESKPEVKSVDIS 155


>At5g55300.1 68418.m06891 DNA topoisomerase I identical to
           Swiss-Prot:P30181 DNA topoisomerase I [Arabidopsis
           thaliana]
          Length = 916

 Score = 27.9 bits (59), Expect = 7.3
 Identities = 10/31 (32%), Positives = 17/31 (54%)
 Frame = +3

Query: 489 TKNERAVPITQTGPVRKEIKEPTAEAESNET 581
           ++  +AVP T+  P+R  +  P     SN+T
Sbjct: 43  SQRSKAVPTTKVSPMRSPVTSPNGTTPSNKT 73


>At1g31440.1 68414.m03850 SH3 domain-containing protein 1 (SH3P1)
           nearly identical to SH3 domain-containing protein 1
           [Arabidopsis thaliana] GI:16974676; contains Pfam
           profile PF00018: SH3 domain
          Length = 439

 Score = 27.9 bits (59), Expect = 7.3
 Identities = 16/38 (42%), Positives = 17/38 (44%)
 Frame = +2

Query: 110 LRTGAYSGRSPDGRHHANDVQRLLPSVEADGDRQ*RRR 223
           +RT  YS    D RH  N   RL   VEA      RRR
Sbjct: 136 IRTMIYSAPLEDARHLVNHYDRLRQEVEAQATDVLRRR 173


>At1g35160.1 68414.m04360 14-3-3 protein GF14 phi (GRF4) identical
           to GF14 protein phi chain GI:1493805, SP:P46077 from
           [Arabidopsis thaliana]
          Length = 267

 Score = 27.5 bits (58), Expect = 9.7
 Identities = 13/46 (28%), Positives = 24/46 (52%)
 Frame = +1

Query: 88  RTAFAIRTSDWRLLRTIS*RPSSRQRCPEITTVRGSRWRSPIETSV 225
           +     R + WR++ +I  +  SR     +TT+R   +RS IE+ +
Sbjct: 59  KNVIGARRASWRIISSIEQKEESRGNDDHVTTIRD--YRSKIESEL 102


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 13,096,861
Number of Sequences: 28952
Number of extensions: 243197
Number of successful extensions: 698
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 685
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 697
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1604469728
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)