BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= brP-0137 (670 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPCC1682.14 |rpl1902|rpl19-2|60S ribosomal protein L19B|Schizosa... 208 4e-55 SPBC56F2.02 |rpl1901|rpl19-1|60S ribosomal protein L19|Schizosac... 208 4e-55 SPBC12C2.02c |ste20|ste16|sterility protein Ste20|Schizosaccharo... 29 0.61 SPBC28E12.04 |||sequence orphan|Schizosaccharomyces pombe|chr 2|... 29 0.80 SPCC1682.02c |mcm3||MCM complex subunit Mcm3|Schizosaccharomyces... 28 1.4 SPBC21.05c |ral2||Ras guanyl-nucleotide exchange factor Ral2 |Sc... 26 5.6 SPAC3H8.02 |||sec14 cytosolic factor family|Schizosaccharomyces ... 25 9.9 SPAC222.09 |seb1||RNA-binding protein Seb1 |Schizosaccharomyces ... 25 9.9 SPAC823.09c |||L-asparaginase |Schizosaccharomyces pombe|chr 1||... 25 9.9 SPAC22F8.11 |plc1||phosphoinositide phospholipase C Plc1|Schizos... 25 9.9 SPAC10F6.03c |||CTP synthase |Schizosaccharomyces pombe|chr 1|||... 25 9.9 >SPCC1682.14 |rpl1902|rpl19-2|60S ribosomal protein L19B|Schizosaccharomyces pombe|chr 3|||Manual Length = 193 Score = 208 bits (509), Expect = 4e-55 Identities = 94/165 (56%), Positives = 124/165 (75%) Frame = +1 Query: 16 MSSLKLQKRLAASVMRCGKKKVWLDPNEINEIANTNSRQNIRKMIKDGLVIKKPVAVHSR 195 M++L+ QKRLAASV++CGK+KVW+DPNEI+EI+N NSRQNIRK++KDGLVI+KP +HSR Sbjct: 1 MANLRTQKRLAASVLKCGKRKVWMDPNEISEISNANSRQNIRKLVKDGLVIRKPNLMHSR 60 Query: 196 ARVRKNTEARRKGRHCGFGKRRGTANARMPQKELWXXXXXXXXXXXXXXXTAKKIDRHLY 375 R+RK A+R GRH G+GKR+GTA ARMP +W + KID+HLY Sbjct: 61 FRIRKTHAAKRLGRHTGYGKRKGTAEARMPSTVVWMRRQRVLRRLLRKYRESGKIDKHLY 120 Query: 376 HSLYMKAKGNVFKNKRVLMEYIHRKKAEKARTKMLSDQAXARRXK 510 H+LY++AKGN FK+KR L+E+I R KAE RTK++ +Q ARR + Sbjct: 121 HTLYLEAKGNTFKHKRALIEHIQRAKAEANRTKLIQEQQDARRAR 165 >SPBC56F2.02 |rpl1901|rpl19-1|60S ribosomal protein L19|Schizosaccharomyces pombe|chr 2|||Manual Length = 193 Score = 208 bits (509), Expect = 4e-55 Identities = 94/165 (56%), Positives = 124/165 (75%) Frame = +1 Query: 16 MSSLKLQKRLAASVMRCGKKKVWLDPNEINEIANTNSRQNIRKMIKDGLVIKKPVAVHSR 195 M++L+ QKRLAASV++CGK+KVW+DPNEI+EI+N NSRQN+RK+IKDGLVI+KP +HSR Sbjct: 1 MANLRTQKRLAASVLKCGKRKVWMDPNEISEISNANSRQNVRKLIKDGLVIRKPNLMHSR 60 Query: 196 ARVRKNTEARRKGRHCGFGKRRGTANARMPQKELWXXXXXXXXXXXXXXXTAKKIDRHLY 375 R+RK A+R GRH G+GKR+GTA ARMP +W + KID+HLY Sbjct: 61 FRIRKTHAAKRLGRHTGYGKRKGTAEARMPSAVVWMRRQRVLRRLLRKYRESGKIDKHLY 120 Query: 376 HSLYMKAKGNVFKNKRVLMEYIHRKKAEKARTKMLSDQAXARRXK 510 H+LY++AKGN FK+KR L+E+I R KAE RTK++ +Q ARR + Sbjct: 121 HTLYLEAKGNTFKHKRALIEHIQRAKAEANRTKLIQEQQDARRAR 165 >SPBC12C2.02c |ste20|ste16|sterility protein Ste20|Schizosaccharomyces pombe|chr 2|||Manual Length = 1309 Score = 29.1 bits (62), Expect = 0.61 Identities = 12/30 (40%), Positives = 21/30 (70%) Frame = -2 Query: 159 TILDHLTDVLSGVGVCDLIDFIWIQPHLLF 70 +I +H++DV G+ V LI+ + I+P +LF Sbjct: 351 SICEHVSDVSRGIAVETLIELMIIRPKILF 380 >SPBC28E12.04 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||Manual Length = 356 Score = 28.7 bits (61), Expect = 0.80 Identities = 12/32 (37%), Positives = 19/32 (59%) Frame = -2 Query: 138 DVLSGVGVCDLIDFIWIQPHLLFTTSHNRGCK 43 +VLS + ++D + PHLL +S N+ CK Sbjct: 237 EVLSKLPYFHILDKSFFSPHLLLQSSENQACK 268 >SPCC1682.02c |mcm3||MCM complex subunit Mcm3|Schizosaccharomyces pombe|chr 3|||Manual Length = 879 Score = 27.9 bits (59), Expect = 1.4 Identities = 12/33 (36%), Positives = 20/33 (60%) Frame = +2 Query: 194 ALVSAKTQKHVERVVTVALVREEVQPMRVCHRR 292 ++V K K E+++ AL RE V+P R H++ Sbjct: 652 SVVEVKDAKAAEKILRYALFREVVKPKRKKHKK 684 >SPBC21.05c |ral2||Ras guanyl-nucleotide exchange factor Ral2 |Schizosaccharomyces pombe|chr 2|||Manual Length = 611 Score = 25.8 bits (54), Expect = 5.6 Identities = 11/32 (34%), Positives = 17/32 (53%), Gaps = 1/32 (3%) Frame = -3 Query: 455 AFFLWMYSMSTRLFLNTLPFAFM-*SEWYRCL 363 AF LW+YS+ L+L + W++CL Sbjct: 337 AFVLWVYSLDKELWLQLDMLGVLNHGSWFKCL 368 >SPAC3H8.02 |||sec14 cytosolic factor family|Schizosaccharomyces pombe|chr 1|||Manual Length = 444 Score = 25.0 bits (52), Expect = 9.9 Identities = 16/65 (24%), Positives = 31/65 (47%), Gaps = 11/65 (16%) Frame = +1 Query: 76 KVWLDPNEINEIANTNSRQNIRKMIKDGLVIKK-----------PVAVHSRARVRKNTEA 222 K WLDP ++++ T + +++++ I ++K+ P + A KN EA Sbjct: 289 KSWLDPVVVSKVKFTRNYRDLQQYINPDNILKEFGGPNPWRYTYPEPCQNEAEALKNVEA 348 Query: 223 RRKGR 237 R+ R Sbjct: 349 RKSLR 353 >SPAC222.09 |seb1||RNA-binding protein Seb1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 620 Score = 25.0 bits (52), Expect = 9.9 Identities = 11/39 (28%), Positives = 20/39 (51%) Frame = +1 Query: 130 QNIRKMIKDGLVIKKPVAVHSRARVRKNTEARRKGRHCG 246 +++R +KD + +PV+V S A ++T G G Sbjct: 141 ESLRSKLKDAMASTEPVSVDSAAAPSQSTNPEGNGGSVG 179 >SPAC823.09c |||L-asparaginase |Schizosaccharomyces pombe|chr 1|||Manual Length = 285 Score = 25.0 bits (52), Expect = 9.9 Identities = 11/34 (32%), Positives = 17/34 (50%) Frame = -1 Query: 340 TSGAIFLKPFVFVPIVPSVAYAHWLYLFSYQSHS 239 +SG + LKP + P + W+ F +SHS Sbjct: 193 SSGGLLLKPAGRIGSSPIPGHGFWIESFDNKSHS 226 >SPAC22F8.11 |plc1||phosphoinositide phospholipase C Plc1|Schizosaccharomyces pombe|chr 1|||Manual Length = 899 Score = 25.0 bits (52), Expect = 9.9 Identities = 11/28 (39%), Positives = 14/28 (50%) Frame = -1 Query: 271 WLYLFSYQSHSDDPFYVLLCFCGHERGS 188 W Y+S D FY ++ F H RGS Sbjct: 824 WGETMVYESIISDDFYSMIRFLVHHRGS 851 >SPAC10F6.03c |||CTP synthase |Schizosaccharomyces pombe|chr 1|||Manual Length = 600 Score = 25.0 bits (52), Expect = 9.9 Identities = 13/44 (29%), Positives = 22/44 (50%), Gaps = 2/44 (4%) Frame = +1 Query: 19 SSLKLQKRLAASVMRCGKK--KVWLDPNEINEIANTNSRQNIRK 144 S + + K L S MRCG+K W++ + + NT+ + K Sbjct: 317 SYISVIKALEHSAMRCGRKLDLQWVEASHLEASTNTSDPLSYHK 360 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,413,156 Number of Sequences: 5004 Number of extensions: 47145 Number of successful extensions: 155 Number of sequences better than 10.0: 11 Number of HSP's better than 10.0 without gapping: 146 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 153 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 305854096 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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