Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= brP-0133
(755 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At4g10250.1 68417.m01682 22.0 kDa ER small heat shock protein (H... 37 0.013
At1g53540.1 68414.m06074 17.6 kDa class I small heat shock prote... 34 0.089
At5g59720.1 68418.m07486 18.1 kDa class I heat shock protein (HS... 33 0.20
At3g46230.1 68416.m05004 17.4 kDa class I heat shock protein (HS... 33 0.20
At2g29500.1 68415.m03583 17.6 kDa class I small heat shock prote... 33 0.27
At2g27100.1 68415.m03256 C2H2 zinc-finger protein SERRATE (SE) i... 32 0.47
At4g21870.1 68417.m03163 26.5 kDa class P-related heat shock pro... 30 1.9
At3g49540.1 68416.m05414 expressed protein 30 1.9
At5g55670.1 68418.m06941 RNA recognition motif (RRM)-containing ... 29 2.5
At2g28680.1 68415.m03486 cupin family protein similar to legumin... 29 3.3
At1g76770.1 68414.m08934 heat shock protein-related contains sim... 29 3.3
At4g36850.1 68417.m05225 PQ-loop repeat family protein / transme... 29 4.4
At4g14830.1 68417.m02280 expressed protein 29 4.4
At5g42820.2 68418.m05216 U2 snRNP auxiliary factor small subunit... 28 5.8
At5g42820.1 68418.m05215 U2 snRNP auxiliary factor small subunit... 28 5.8
At5g18190.1 68418.m02135 protein kinase family protein contains ... 28 5.8
At3g56100.1 68416.m06235 leucine-rich repeat transmembrane prote... 28 5.8
At2g20360.1 68415.m02377 expressed protein 28 5.8
At4g17520.1 68417.m02621 nuclear RNA-binding protein, putative s... 28 7.7
At1g73710.1 68414.m08535 pentatricopeptide (PPR) repeat-containi... 28 7.7
>At4g10250.1 68417.m01682 22.0 kDa ER small heat shock protein
(HSP22.0-ER) identical to endomembrane-localized small
heat shock protein GI:511795 from [Arabidopsis thaliana]
Length = 195
Score = 37.1 bits (82), Expect = 0.013
Identities = 20/48 (41%), Positives = 31/48 (64%)
Frame = +1
Query: 403 QFVRRYALPEXAAPETVESRLSSDGVLTITAPRKVPDAVKGERKVPIA 546
+F R++ LP+ E+V+++L + GVLTI + P+ VKG R V IA
Sbjct: 132 KFWRQFKLPDNVDMESVKAKLEN-GVLTINLTKLSPEKVKGPRVVNIA 178
>At1g53540.1 68414.m06074 17.6 kDa class I small heat shock protein
(HSP17.6C-CI) (AA 1-156) identical to (17.6 kDa class I
heat shock protein (HSP 17.6) (AA 1-156)(SP:P13853)
(GI:4376161) (Arabidopsis thaliana) (Nucleic Acids Res.
17 (19), 7995 (1989))
Length = 157
Score = 34.3 bits (75), Expect = 0.089
Identities = 18/50 (36%), Positives = 31/50 (62%)
Frame = +1
Query: 397 SRQFVRRYALPEXAAPETVESRLSSDGVLTITAPRKVPDAVKGERKVPIA 546
S +F RR+ LPE A E +++ + +GVL++T P KVP+ + + I+
Sbjct: 109 SGKFTRRFRLPENAKMEEIKASM-ENGVLSVTVP-KVPEKKPEVKSIDIS 156
>At5g59720.1 68418.m07486 18.1 kDa class I heat shock protein
(HSP18.1-CI) identical to 18.2 kDa class I heat shock
protein (HSP 18.2) (SP:P19037)[Arabidopsis thaliana];
contains Pfam profile: PF00011 Hsp20/alpha crystallin
family
Length = 161
Score = 33.1 bits (72), Expect = 0.20
Identities = 18/50 (36%), Positives = 30/50 (60%)
Frame = +1
Query: 397 SRQFVRRYALPEXAAPETVESRLSSDGVLTITAPRKVPDAVKGERKVPIA 546
S +F+RR+ LPE A E V++ + +GVLT+ P K P+ + + I+
Sbjct: 111 SGKFMRRFRLPENAKMEEVKATM-ENGVLTVVVP-KAPEKKPQVKSIDIS 158
>At3g46230.1 68416.m05004 17.4 kDa class I heat shock protein
(HSP17.4-CI) identical to 17.4 kDa class I heat shock
protein SP:P19036 from [Arabidopsis thaliana]
Length = 156
Score = 33.1 bits (72), Expect = 0.20
Identities = 19/50 (38%), Positives = 32/50 (64%)
Frame = +1
Query: 397 SRQFVRRYALPEXAAPETVESRLSSDGVLTITAPRKVPDAVKGERKVPIA 546
S +F+RR+ LPE A E V++ + +GVL++T P KV ++ + V I+
Sbjct: 108 SGKFMRRFRLPENAKVEEVKASM-ENGVLSVTVP-KVQESKPEVKSVDIS 155
>At2g29500.1 68415.m03583 17.6 kDa class I small heat shock protein
(HSP17.6B-CI) contains Pfam PF00011: Hsp20/alpha
crystallin family; identified in Scharf, K-D., et al,
Cell Stress & Chaperones (2001) 6: 225-237.
Length = 153
Score = 32.7 bits (71), Expect = 0.27
Identities = 15/35 (42%), Positives = 23/35 (65%)
Frame = +1
Query: 397 SRQFVRRYALPEXAAPETVESRLSSDGVLTITAPR 501
S QF RR+ LPE + V++ + +GVLT+T P+
Sbjct: 105 SGQFTRRFRLPENVKMDQVKAAM-ENGVLTVTVPK 138
>At2g27100.1 68415.m03256 C2H2 zinc-finger protein SERRATE (SE)
identical to C2H2 zinc-finger protein SERRATE
GI:14486602 from [Arabidopsis thaliana]
Length = 720
Score = 31.9 bits (69), Expect = 0.47
Identities = 15/32 (46%), Positives = 16/32 (50%)
Frame = +3
Query: 504 GTRRRQGREKGAHRTDRSRSQGDQGPERGNPG 599
G R R GR G R DRS Q + GNPG
Sbjct: 582 GRRERDGRANGNDRNDRSEDQQRGDNDGGNPG 613
>At4g21870.1 68417.m03163 26.5 kDa class P-related heat shock
protein (HSP26.5-P) contains Pfam profile: PF00011
Hsp20/alpha crystallin family: identified in Scharf,
K-D., et al,Cell Stress & Chaperones (2001) 6: 225-237.
Length = 134
Score = 29.9 bits (64), Expect = 1.9
Identities = 18/79 (22%), Positives = 33/79 (41%)
Frame = +1
Query: 271 DKFQVNLDVQHFSPEEISVKTADGYIVVXXXXXXXXXXXXYISRQFVRRYALPEXAAPET 450
D ++D+ EEI V+ D ++ + F R++ LPE
Sbjct: 35 DSHTFSVDLPGLRKEEIKVEIEDSIYLIIRTEATPMSPPDQPLKTFKRKFRLPESIDMIG 94
Query: 451 VESRLSSDGVLTITAPRKV 507
+ + DGVLT+ P+++
Sbjct: 95 ISAGYE-DGVLTVIVPKRI 112
>At3g49540.1 68416.m05414 expressed protein
Length = 166
Score = 29.9 bits (64), Expect = 1.9
Identities = 20/65 (30%), Positives = 35/65 (53%), Gaps = 4/65 (6%)
Frame = +1
Query: 430 EXAAPETVESRLSSDGVLTITAPRKVPDAVKGERKV---PI-AQTGPVRKEIKDQSEGTQ 597
E AA E E+++ + + + AP KV AV+ E+K P+ A+ PV+ E + E ++
Sbjct: 100 ENAATENAEAKVEA---VAVAAPEKVEVAVEAEKKAEAEPVKAEAEPVKAEAEPVKEESK 156
Query: 598 DAENK 612
E +
Sbjct: 157 QEEKE 161
>At5g55670.1 68418.m06941 RNA recognition motif (RRM)-containing
protein
Length = 710
Score = 29.5 bits (63), Expect = 2.5
Identities = 15/39 (38%), Positives = 20/39 (51%), Gaps = 5/39 (12%)
Frame = +3
Query: 471 RRGSHHHRAEEGTR-----RRQGREKGAHRTDRSRSQGD 572
R HHH+ E +R R + RE+ HR +R R GD
Sbjct: 619 REREHHHKDRERSREHVRDRERERERDRHREERERYGGD 657
>At2g28680.1 68415.m03486 cupin family protein similar to legumin
(11S-globulin) from Ginkgo biloba [GI:949869], 11S
globulin from Avena sativa [GI:472867]; contains a 11-S
plant seed storage protein signature (PS00305)
Length = 356
Score = 29.1 bits (62), Expect = 3.3
Identities = 21/64 (32%), Positives = 35/64 (54%), Gaps = 3/64 (4%)
Frame = +1
Query: 397 SRQFV-RRYALPEXAAPETVESRLSSDGVLTITAPRKVPDAVKGERK--VPIAQTGPVRK 567
S +FV R + L E + V S+ + +G++ + A K+P+ KG+RK V P+
Sbjct: 141 STEFVGRAWDLDETTVKKLVGSQ-TGNGIVKVDASLKMPEPKKGDRKGFVLNCLEAPLDV 199
Query: 568 EIKD 579
+IKD
Sbjct: 200 DIKD 203
>At1g76770.1 68414.m08934 heat shock protein-related contains
similarity to 17.9 kDa heat-shock protein [Helianthus
annuus] gi|11990130|emb|CAB55634
Length = 244
Score = 29.1 bits (62), Expect = 3.3
Identities = 11/37 (29%), Positives = 24/37 (64%), Gaps = 1/37 (2%)
Frame = +1
Query: 400 RQFVRRYALPEXAAPETVESRLSS-DGVLTITAPRKV 507
++F + + +P+ + +++R + DG LT+T P+KV
Sbjct: 93 KEFKKVFRIPDIVILDKIKARFNEEDGTLTVTMPKKV 129
>At4g36850.1 68417.m05225 PQ-loop repeat family protein /
transmembrane family protein similar to SP|Q10482 Seven
transmembrane protein 1 {Schizosaccharomyces pombe};
contains Pfam profile PF04193: PQ loop repeat
Length = 374
Score = 28.7 bits (61), Expect = 4.4
Identities = 13/52 (25%), Positives = 30/52 (57%), Gaps = 2/52 (3%)
Frame = -1
Query: 611 LFSASWVPSLWSLISLRTGPVCAMGT--FLSPLTASGTFLGAVMVRTPSDDS 462
++ +P +W ++++ G V + F+ L A+ T++G+++VRT D+
Sbjct: 269 IYMGGRIPQIW--LNIKRGSVEGLNPLMFIFALVANATYVGSILVRTTEWDN 318
>At4g14830.1 68417.m02280 expressed protein
Length = 152
Score = 28.7 bits (61), Expect = 4.4
Identities = 13/31 (41%), Positives = 16/31 (51%)
Frame = +1
Query: 409 VRRYALPEXAAPETVESRLSSDGVLTITAPR 501
V R+ LPE PE V DG L +T P+
Sbjct: 109 VWRFRLPESTRPELVTVDCDGDGELIVTVPK 139
>At5g42820.2 68418.m05216 U2 snRNP auxiliary factor small subunit,
putative strong similarity to U2 snRNP auxiliary factor,
small subunit [Oryza sativa] GI:3850816
Length = 283
Score = 28.3 bits (60), Expect = 5.8
Identities = 19/43 (44%), Positives = 22/43 (51%)
Frame = +2
Query: 407 SSDVTRCLXARRLRLWNRGCHQTGFSPSPRRGRYPTPSRERER 535
S ++ R L R R + RG S SPRR R SRERER
Sbjct: 177 SRELRRKLFGRYRRSYRRGSRSRSRSISPRRKR--EHSRERER 217
>At5g42820.1 68418.m05215 U2 snRNP auxiliary factor small subunit,
putative strong similarity to U2 snRNP auxiliary factor,
small subunit [Oryza sativa] GI:3850816
Length = 283
Score = 28.3 bits (60), Expect = 5.8
Identities = 19/43 (44%), Positives = 22/43 (51%)
Frame = +2
Query: 407 SSDVTRCLXARRLRLWNRGCHQTGFSPSPRRGRYPTPSRERER 535
S ++ R L R R + RG S SPRR R SRERER
Sbjct: 177 SRELRRKLFGRYRRSYRRGSRSRSRSISPRRKR--EHSRERER 217
>At5g18190.1 68418.m02135 protein kinase family protein contains
Pfam domains, PF00069: Protein kinase domain
Length = 691
Score = 28.3 bits (60), Expect = 5.8
Identities = 11/22 (50%), Positives = 12/22 (54%)
Frame = +3
Query: 207 QTVETTRGCIS*PRLQHKGRQG 272
Q T GC P + HKGRQG
Sbjct: 185 QVYNTLNGCYGVPAVHHKGRQG 206
>At3g56100.1 68416.m06235 leucine-rich repeat transmembrane protein
kinase, putative hypothetical proteins - Arabidopsis
thaliana
Length = 719
Score = 28.3 bits (60), Expect = 5.8
Identities = 13/25 (52%), Positives = 16/25 (64%)
Frame = +2
Query: 476 GFSPSPRRGRYPTPSRERERCPSHR 550
G+S S P+PS E+ER PSHR
Sbjct: 342 GYSVSTPCPTLPSPSPEKERKPSHR 366
>At2g20360.1 68415.m02377 expressed protein
Length = 402
Score = 28.3 bits (60), Expect = 5.8
Identities = 13/26 (50%), Positives = 16/26 (61%)
Frame = -3
Query: 561 NGTGLCDGHLSLSLDGVGYLPRRGDG 484
NGT C SL+ GVG+L R+G G
Sbjct: 36 NGTDNCRYSSSLATKGVGHLARKGTG 61
>At4g17520.1 68417.m02621 nuclear RNA-binding protein, putative
similar to nuclear RNA binding protein GI:6492264 from
[Arabidopsis thaliana]
Length = 360
Score = 27.9 bits (59), Expect = 7.7
Identities = 15/44 (34%), Positives = 21/44 (47%)
Frame = +3
Query: 477 GSHHHRAEEGTRRRQGREKGAHRTDRSRSQGDQGPERGNPGCRK 608
G + R+EEG R+G G +R DR S + G + RK
Sbjct: 95 GGGYRRSEEGDGARRGGPVGGYRGDRRGSYSNGGDSGDSERPRK 138
>At1g73710.1 68414.m08535 pentatricopeptide (PPR) repeat-containing
protein low similarity to fertility restorer [Petunia x
hybrida] GI:22128587, post-transcriptional control of
chloroplast gene expression CRP1 [Zea mays] GI:3289002;
contains Pfam profile PF01535: PPR repeat
Length = 991
Score = 27.9 bits (59), Expect = 7.7
Identities = 10/27 (37%), Positives = 15/27 (55%)
Frame = -3
Query: 552 GLCDGHLSLSLDGVGYLPRRGDGENPV 472
G C G + L LD + P+ G ++PV
Sbjct: 240 GWCAGKVDLDLDSIDDFPKNGSAQSPV 266
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 15,898,466
Number of Sequences: 28952
Number of extensions: 334880
Number of successful extensions: 1109
Number of sequences better than 10.0: 20
Number of HSP's better than 10.0 without gapping: 1051
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1108
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1682736544
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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