Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= brP-0131
(774 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC23H4.06 |gln1||glutamate-ammonia ligase Gln1|Schizosaccharom... 32 0.10
SPAC227.15 |||protein phosphatase regulatory subunit Reg1 |Schiz... 28 1.3
SPCC1322.10 |||conserved fungal protein|Schizosaccharomyces pomb... 27 2.3
SPAC343.07 |mug28||RNA-binding protein Mug28|Schizosaccharomyces... 27 3.0
SPAC17D4.03c |||membrane transporter |Schizosaccharomyces pombe|... 27 3.0
SPBC887.14c |pfh1|pif1|pif1 helicase homolog Pfh1|Schizosaccharo... 27 3.0
SPBC1815.01 |eno101|eno1|enolase|Schizosaccharomyces pombe|chr 2... 27 3.9
SPAPB15E9.01c ||SPAPB18E9.06c|sequence orphan|Schizosaccharomyce... 27 3.9
SPBC30B4.01c |wsc1|SPBC3D6.14c|transmembrane receptor Wsc1 |Schi... 27 3.9
SPCC18.02 |||membrane transporter|Schizosaccharomyces pombe|chr ... 26 5.2
SPAC23H3.13c |gpa2|git8|heterotrimeric G protein alpha-2 subunit... 26 6.9
SPAC31G5.09c |spk1||MAP kinase Spk1|Schizosaccharomyces pombe|ch... 26 6.9
SPBC3D6.05 |ptp4||phosphatidate cytidylyltransferase Ptp4 |Schiz... 26 6.9
SPAC12G12.01c ||SPAC630.02|ubiquitin-protein ligase E3|Schizosac... 25 9.1
>SPAC23H4.06 |gln1||glutamate-ammonia ligase
Gln1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 359
Score = 31.9 bits (69), Expect = 0.10
Identities = 16/33 (48%), Positives = 20/33 (60%)
Frame = -2
Query: 350 RLTYSRF*LRMAAEDFGVEVLVRPKAEPVLDGW 252
+L SRF L AEDFGV++ PK P+L W
Sbjct: 218 QLWMSRFLLHRIAEDFGVKISFHPK--PILGDW 248
>SPAC227.15 |||protein phosphatase regulatory subunit Reg1
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 873
Score = 28.3 bits (60), Expect = 1.3
Identities = 15/36 (41%), Positives = 20/36 (55%)
Frame = +2
Query: 335 SSRSDGFVIDEDLTDDSLQYLTDDVKELIKIARNPG 442
SS+S F I EDL D L+Y +D + +R PG
Sbjct: 540 SSQSSHFRIIEDLPDSKLKYSLEDQATYDQSSRYPG 575
>SPCC1322.10 |||conserved fungal protein|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 262
Score = 27.5 bits (58), Expect = 2.3
Identities = 20/56 (35%), Positives = 26/56 (46%), Gaps = 2/56 (3%)
Frame = -1
Query: 501 ADMGSGPDLNPSQTSTTLSSPGLRAI--LISSFTSSVKYCKLSSVRSSSITKPSDL 340
A S PS +STT SS + + SS TSS+ SSV SSS + +
Sbjct: 139 ASSTSSSTATPSSSSTTSSSSSSSSSTPISSSITSSISSSASSSVSSSSASSSGSI 194
>SPAC343.07 |mug28||RNA-binding protein Mug28|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 609
Score = 27.1 bits (57), Expect = 3.0
Identities = 11/23 (47%), Positives = 14/23 (60%)
Frame = +2
Query: 233 TEASLLSIHQEQVQPLVEPRPQH 301
TE +L HQE QPL++ QH
Sbjct: 530 TEPQILEYHQEWPQPLIQSIQQH 552
>SPAC17D4.03c |||membrane transporter |Schizosaccharomyces pombe|chr
1|||Manual
Length = 732
Score = 27.1 bits (57), Expect = 3.0
Identities = 14/45 (31%), Positives = 23/45 (51%)
Frame = +3
Query: 84 SQHVPTNQDSTRRHHHQASQTDSTITGNDLSCYHLNKRLSTLSSD 218
SQH T+++S + HHH+ + I + C H + L L +D
Sbjct: 528 SQHEHTHENS-QEHHHEHNHNHDHIHKYNEKCDHESISLQNLDND 571
>SPBC887.14c |pfh1|pif1|pif1 helicase homolog
Pfh1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 805
Score = 27.1 bits (57), Expect = 3.0
Identities = 20/65 (30%), Positives = 30/65 (46%), Gaps = 4/65 (6%)
Frame = +3
Query: 48 PYPQRQPQSHQNSQHVPTNQDSTRRHHHQASQTDSTI---TGNDLSCYHLNKRLST-LSS 215
P P+R S SQH +N S + + ++S DS S ++K+ S L+S
Sbjct: 225 PDPKRTSTSADISQHTVSNDSSNKLSNGRSSSLDSLAKKRMSKSKSTPQISKKFSVPLNS 284
Query: 216 DRKSP 230
KSP
Sbjct: 285 ASKSP 289
>SPBC1815.01 |eno101|eno1|enolase|Schizosaccharomyces pombe|chr
2|||Manual
Length = 439
Score = 26.6 bits (56), Expect = 3.9
Identities = 16/43 (37%), Positives = 25/43 (58%)
Frame = +3
Query: 96 PTNQDSTRRHHHQASQTDSTITGNDLSCYHLNKRLSTLSSDRK 224
P +QD H ++TD I G+DL+ ++ KRL T + D+K
Sbjct: 297 PFDQDDWSAWTHMKAETDFQIVGDDLTVTNV-KRLRT-AIDKK 337
>SPAPB15E9.01c ||SPAPB18E9.06c|sequence orphan|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1036
Score = 26.6 bits (56), Expect = 3.9
Identities = 26/68 (38%), Positives = 33/68 (48%), Gaps = 4/68 (5%)
Frame = -1
Query: 513 SFALADMGSGPDLNPSQT-STTLSSPGLRAILISSFTSS---VKYCKLSSVRSSSITKPS 346
S A + + S L S T STT +SP ++ SS TSS SS+ SSSIT S
Sbjct: 79 SAASSSLTSSSSLASSSTNSTTSASPTSSSLTSSSATSSSLASSSTTSSSLASSSITSSS 138
Query: 345 DLLEILTS 322
+TS
Sbjct: 139 LASSSITS 146
>SPBC30B4.01c |wsc1|SPBC3D6.14c|transmembrane receptor Wsc1
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 374
Score = 26.6 bits (56), Expect = 3.9
Identities = 19/70 (27%), Positives = 31/70 (44%)
Frame = -1
Query: 513 SFALADMGSGPDLNPSQTSTTLSSPGLRAILISSFTSSVKYCKLSSVRSSSITKPSDLLE 334
S + + S + S +S++ SS + SS +SS SS SSS ++PS
Sbjct: 180 SSSSSSSSSSSSSSSSSSSSSSSSSSSSVPITSSTSSSHSSSSSSSSSSSSSSRPSSSSS 239
Query: 333 ILTSYGGRRF 304
+T+ F
Sbjct: 240 FITTMSSSTF 249
>SPCC18.02 |||membrane transporter|Schizosaccharomyces pombe|chr
3|||Manual
Length = 448
Score = 26.2 bits (55), Expect = 5.2
Identities = 10/34 (29%), Positives = 22/34 (64%)
Frame = +2
Query: 170 FILLPLEQATQYTVIRPQEPKTEASLLSIHQEQV 271
FI+L L+ A ++ +I P+E K +++L + + +
Sbjct: 182 FIILLLDIALRFLMIEPREMKNDSTLQDVERTSI 215
>SPAC23H3.13c |gpa2|git8|heterotrimeric G protein alpha-2 subunit
Gpa2 |Schizosaccharomyces pombe|chr 1|||Manual
Length = 354
Score = 25.8 bits (54), Expect = 6.9
Identities = 19/90 (21%), Positives = 40/90 (44%)
Frame = +3
Query: 90 HVPTNQDSTRRHHHQASQTDSTITGNDLSCYHLNKRLSTLSSDRKSPRQKPHCFPSIKNR 269
+VPT D R + ++ + T + HL R+ + R R+ +CF ++ +
Sbjct: 165 YVPTIDDILRSRNSTLGISEISFTLD-----HLQIRMFDVGGQRTERRKWIYCFENVNSI 219
Query: 270 FSLWSNQDLNTKIFCRHTKSKSRVGQTALL 359
S D + K++ R ++R+ ++ L
Sbjct: 220 IFCVSLNDYDKKLYERAAPERNRLVESISL 249
>SPAC31G5.09c |spk1||MAP kinase Spk1|Schizosaccharomyces pombe|chr
1|||Manual
Length = 372
Score = 25.8 bits (54), Expect = 6.9
Identities = 15/54 (27%), Positives = 25/54 (46%), Gaps = 2/54 (3%)
Frame = +2
Query: 287 PRPQHQNLLPPYEVKISSRSDGFVI--DEDLTDDSLQYLTDDVKELIKIARNPG 442
P P +Q L Y V+ +D + + + L+DD QY T + +K + G
Sbjct: 105 PPPSYQELEDVYIVQELMETDLYRVIRSQPLSDDHCQYFTYQILRALKAMHSAG 158
>SPBC3D6.05 |ptp4||phosphatidate cytidylyltransferase Ptp4
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 218
Score = 25.8 bits (54), Expect = 6.9
Identities = 8/10 (80%), Positives = 9/10 (90%)
Frame = -3
Query: 751 QLRLPFPWLC 722
Q R+PFPWLC
Sbjct: 173 QSRIPFPWLC 182
>SPAC12G12.01c ||SPAC630.02|ubiquitin-protein ligase
E3|Schizosaccharomyces pombe|chr 1|||Manual
Length = 905
Score = 25.4 bits (53), Expect = 9.1
Identities = 18/56 (32%), Positives = 28/56 (50%), Gaps = 5/56 (8%)
Frame = +1
Query: 208 CHQTARAQDRSLIA-FHPSRTGSAFG----RTKTSTPKSSAAIRSQNLE*VRRLCY 360
C+ A ++ L+A F SR S+ + S PKSS ++S L+ V +CY
Sbjct: 91 CNSLAFSETEHLVAGFAKSRNESSLKLWDLNSLLSDPKSSPLMQSSTLDGVSSVCY 146
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,066,625
Number of Sequences: 5004
Number of extensions: 61654
Number of successful extensions: 289
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 256
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 287
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 373338084
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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