BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= brP-0129 (625 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 363 e-101 SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 359 e-100 SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 159 3e-40 SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 93 3e-20 SPAPB18E9.04c |||sequence orphan|Schizosaccharomyces pombe|chr 1... 32 0.059 SPAPJ760.02c |app1||App1 protein|Schizosaccharomyces pombe|chr 1... 31 0.14 SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||... 29 0.55 SPAC19D5.04 |ptr1||HECT domain|Schizosaccharomyces pombe|chr 1||... 29 0.72 SPCC18.03 |||shuttle craft like transcriptional regulator|Schizo... 28 0.95 SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual 28 1.3 SPAC1006.06 |rgf2||RhoGEF Rgf2|Schizosaccharomyces pombe|chr 1||... 26 3.8 SPAC1B3.15c |||membrane transporter|Schizosaccharomyces pombe|ch... 26 3.8 SPBC428.10 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||... 26 5.1 SPAC30D11.13 |hus5|ubc9|SUMO conjugating enzyme Hus5|Schizosacch... 26 5.1 SPAC890.02c |alp7|mia1|TACC homolog |Schizosaccharomyces pombe|c... 26 5.1 SPAC222.10c |byr4||two-component GAP Byr4|Schizosaccharomyces po... 25 6.7 SPAC23E2.02 |lsd2|swm2, saf140|histone demethylase SWIRM2 |Schiz... 25 6.7 SPBC3B8.10c |||NLI interacting factor family|Schizosaccharomyces... 25 8.9 SPAC13G6.10c |||O-glucosyl hydrolase |Schizosaccharomyces pombe|... 25 8.9 >SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr 2|||Manual Length = 455 Score = 363 bits (892), Expect = e-101 Identities = 162/208 (77%), Positives = 182/208 (87%) Frame = +2 Query: 2 DIERPTYTNLNRLIGQIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLVTYAPVIS 181 DIERPTY NLNRLI Q+VSSITASLRF G+LNVDL EFQTNLVPYPRIHFPLVTY+P++S Sbjct: 222 DIERPTYENLNRLIAQVVSSITASLRFAGSLNVDLNEFQTNLVPYPRIHFPLVTYSPIVS 281 Query: 182 AEKAYHEQLSVAEITNACFEPANQMVKCDPRHGKYMACCMLYRGDVVPKDVNAAIATIKT 361 A KA+HE SV EITN CFEP NQMVKCDPR G+YMA C+LYRGDV+P+DV AA+ +IK+ Sbjct: 282 AAKAFHESNSVQEITNQCFEPYNQMVKCDPRTGRYMATCLLYRGDVIPRDVQAAVTSIKS 341 Query: 362 KRTIQFVDWCPTGFKVGINYQPPTVVPGGDLAXVQRAVCMLSNTTAIAEAWARLDHKFDL 541 +RTIQFVDWCPTGFK+GI Y+PP VPG +A V RAVCMLSNTT+IAEAW+RLDHKFDL Sbjct: 342 RRTIQFVDWCPTGFKIGICYEPPQHVPGSGIAKVNRAVCMLSNTTSIAEAWSRLDHKFDL 401 Query: 542 MYAKRAFVHWYVGXGMXXGXFSEARXDL 625 MY+KRAFVHWYVG GM G FSEAR DL Sbjct: 402 MYSKRAFVHWYVGEGMEEGEFSEAREDL 429 >SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosaccharomyces pombe|chr 2|||Manual Length = 449 Score = 359 bits (884), Expect = e-100 Identities = 161/208 (77%), Positives = 181/208 (87%) Frame = +2 Query: 2 DIERPTYTNLNRLIGQIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLVTYAPVIS 181 DIERP+Y NLNRLI Q+VSSITASLRF+G+LNVDL EFQTNLVPYPRIHFPLVTYAP++S Sbjct: 218 DIERPSYENLNRLIAQVVSSITASLRFEGSLNVDLAEFQTNLVPYPRIHFPLVTYAPIVS 277 Query: 182 AEKAYHEQLSVAEITNACFEPANQMVKCDPRHGKYMACCMLYRGDVVPKDVNAAIATIKT 361 A KA+HE SV EITN CFEP NQMVKCDPR G+YMA C+LYRGDV+P+DV AA+ TIK Sbjct: 278 AAKAFHESNSVQEITNQCFEPYNQMVKCDPRAGRYMATCLLYRGDVIPRDVQAAVTTIKA 337 Query: 362 KRTIQFVDWCPTGFKVGINYQPPTVVPGGDLAXVQRAVCMLSNTTAIAEAWARLDHKFDL 541 KRTIQFVDWCPTGFK+GI +PP + G ++A V RAVCMLSNTT+IAEAW+RLDHKFDL Sbjct: 338 KRTIQFVDWCPTGFKIGICDRPPQHIEGSEIAKVDRAVCMLSNTTSIAEAWSRLDHKFDL 397 Query: 542 MYAKRAFVHWYVGXGMXXGXFSEARXDL 625 MY+KRAFVHWYVG GM G FSEAR DL Sbjct: 398 MYSKRAFVHWYVGEGMEEGEFSEAREDL 425 >SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces pombe|chr 2|||Manual Length = 448 Score = 159 bits (386), Expect = 3e-40 Identities = 75/207 (36%), Positives = 121/207 (58%) Frame = +2 Query: 5 IERPTYTNLNRLIGQIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLVTYAPVISA 184 I+ P+Y +LN L+ +++ +T S RF G LN DL + N+VP+PR+HF +V +AP+ + Sbjct: 217 IKSPSYDDLNHLVSAVMAGVTTSFRFPGELNSDLRKLAVNMVPFPRLHFFMVGFAPLAAI 276 Query: 185 EKAYHEQLSVAEITNACFEPANQMVKCDPRHGKYMACCMLYRGDVVPKDVNAAIATIKTK 364 + + +SV E+T F+ N MV DPRHG+Y+ L+RG V K+V+ I +++TK Sbjct: 277 GSSSFQAVSVPELTQQMFDANNMMVAADPRHGRYLTVAALFRGKVSMKEVDEQIRSVQTK 336 Query: 365 RTIQFVDWCPTGFKVGINYQPPTVVPGGDLAXVQRAVCMLSNTTAIAEAWARLDHKFDLM 544 + FV+W P + PP DL + + + N+T+I E + RL +F M Sbjct: 337 NSAYFVEWIPDNVLKAVCSVPPK-----DL---KMSATFIGNSTSIQEIFRRLGDQFSAM 388 Query: 545 YAKRAFVHWYVGXGMXXGXFSEARXDL 625 + ++AF+HWY G GM F+EA ++ Sbjct: 389 FRRKAFLHWYTGEGMDEMEFTEAESNM 415 >SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr 2|||Manual Length = 446 Score = 93.1 bits (221), Expect = 3e-20 Identities = 51/193 (26%), Positives = 99/193 (51%), Gaps = 4/193 (2%) Frame = +2 Query: 8 ERPTYTNLNRLIGQIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLVTYAPVIS-- 181 + PT+ N+L+ ++S+ T +LR+ G +N DL +L+P PR HF L +Y P + Sbjct: 221 QNPTFHQQNQLVSTVMSASTTTLRYPGYMNNDLVSIIASLIPSPRCHFLLTSYTPFTNQQ 280 Query: 182 -AEKAYHEQLSVAEITNACFEPANQMVKCDP-RHGKYMACCMLYRGDVVPKDVNAAIATI 355 E + +V ++ P NQMV +P + +++ + +G+ P DV+ ++ I Sbjct: 281 VEEAKAIRKTTVLDVMRRLLLPKNQMVSVNPSKKSCFISILDIIQGEADPADVHKSLLRI 340 Query: 356 KTKRTIQFVDWCPTGFKVGINYQPPTVVPGGDLAXVQRAVCMLSNTTAIAEAWARLDHKF 535 + +R F+ W P +V ++ + P + ++ + ML+N T+IA + R ++ Sbjct: 341 RERRYASFIPWGPASIQVALSKKSPYIKTNHRVSGL-----MLANHTSIASLFKRTLDQY 395 Query: 536 DLMYAKRAFVHWY 574 D + + AF+ Y Sbjct: 396 DRLRKRNAFLEQY 408 >SPAPB18E9.04c |||sequence orphan|Schizosaccharomyces pombe|chr 1|||Manual Length = 800 Score = 32.3 bits (70), Expect = 0.059 Identities = 29/112 (25%), Positives = 48/112 (42%), Gaps = 2/112 (1%) Frame = +3 Query: 102 TSPSSRLTWCLTPVSTSHWSRTRQSSLPRRPTM--NSFPSPRSQTHASSPPTRW*NATPV 275 TS S L+ + +++ T S+ P PT+ S +P + T+ ++ + +TPV Sbjct: 381 TSSSPLLSTSTSCTTSTSIPPTGNSTTPVTPTVPPTSSSTPLTTTNCTTSTSVPYTSTPV 440 Query: 276 MASTWLAVCCTVVTSYPRM*TRPSLPSKPSVLSNSSTGVQPVSRSVSTTSHP 431 ++ CT TS P T + S P +N +T S TS P Sbjct: 441 TSTPLATTNCTTSTSVPY--TSTPVTSTPLTTTNCTTSTSIPYTSTPVTSTP 490 Score = 30.3 bits (65), Expect = 0.24 Identities = 29/105 (27%), Positives = 50/105 (47%) Frame = +3 Query: 111 SSRLTWCLTPVSTSHWSRTRQSSLPRRPTMNSFPSPRSQTHASSPPTRW*NATPVMASTW 290 S+ + + TPV++S++ T SS P T P + T+ ++ + +TPV ++ Sbjct: 499 STSVPYTSTPVTSSNY--TISSSTPVTST------PVTTTNCTTSTSVLYTSTPVTSTPL 550 Query: 291 LAVCCTVVTSYPRM*TRPSLPSKPSVLSNSSTGVQPVSRSVSTTS 425 CT TS P T P S ++ S++ PV+ + TTS Sbjct: 551 ATTNCTTSTSVPYTST-PVTSSNYTISSSTPVTSTPVTTTNCTTS 594 Score = 28.3 bits (60), Expect = 0.95 Identities = 27/107 (25%), Positives = 48/107 (44%) Frame = +3 Query: 105 SPSSRLTWCLTPVSTSHWSRTRQSSLPRRPTMNSFPSPRSQTHASSPPTRW*NATPVMAS 284 +P+ T TP++T++ T +S+P T + +P + T+ ++ + +TPV ++ Sbjct: 410 TPTVPPTSSSTPLTTTNC--TTSTSVPYTSTPVT-STPLATTNCTTSTSVPYTSTPVTST 466 Query: 285 TWLAVCCTVVTSYPRM*TRPSLPSKPSVLSNSSTGVQPVSRSVSTTS 425 CT TS P T + S P +N +T S TS Sbjct: 467 PLTTTNCTTSTSIPY--TSTPVTSTPLTTTNCTTSTSVPYTSTPVTS 511 Score = 25.8 bits (54), Expect = 5.1 Identities = 27/98 (27%), Positives = 49/98 (50%), Gaps = 1/98 (1%) Frame = +3 Query: 135 TPVSTSHWSRTRQSSLPRRPTMNSFPSPRSQTHASSPPTRW*NATPVMASTWLAVCCTVV 314 TP++T++ T +S+P T + +P + T+ ++ + +TPV +S + T V Sbjct: 466 TPLTTTNC--TTSTSIPYTSTPVT-STPLTTTNCTTSTSVPYTSTPVTSSNYTISSSTPV 522 Query: 315 TSYPRM*TRPSLPSKPSVLSNSSTGVQ-PVSRSVSTTS 425 TS P T + + SVL S+ P++ + TTS Sbjct: 523 TSTPV--TTTNCTTSTSVLYTSTPVTSTPLATTNCTTS 558 >SPAPJ760.02c |app1||App1 protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 857 Score = 31.1 bits (67), Expect = 0.14 Identities = 24/89 (26%), Positives = 33/89 (37%) Frame = +3 Query: 177 SLPRRPTMNSFPSPRSQTHASSPPTRW*NATPVMASTWLAVCCTVVTSYPRM*TRPSLPS 356 S+P+ P P S + P P + S VV P + RP++P Sbjct: 513 SVPQPPAAPVVPEAPSVHQPPAAPV-----APEVPSAPQRPAAPVVPEAPSVPQRPAVPV 567 Query: 357 KPSVLSNSSTGVQPVSRSVSTTSHPPWCP 443 P LS V PV+ V + PP P Sbjct: 568 VPEALSVPQPPVAPVAPEVPSVPQPPVAP 596 Score = 25.4 bits (53), Expect = 6.7 Identities = 23/90 (25%), Positives = 31/90 (34%) Frame = +3 Query: 177 SLPRRPTMNSFPSPRSQTHASSPPTRW*NATPVMASTWLAVCCTVVTSYPRM*TRPSLPS 356 S+P+RP + P S P P + +A S P+ P P Sbjct: 558 SVPQRPAVPVVPEALSVPQPPVAPVA--PEVPSVPQPPVAPVVPEAPSVPQPPVAPVAPE 615 Query: 357 KPSVLSNSSTGVQPVSRSVSTTSHPPWCPE 446 PSV + V P + SV P PE Sbjct: 616 VPSVPQRPAVPVVPEAPSVPQPPAAPVVPE 645 Score = 25.0 bits (52), Expect = 8.9 Identities = 14/45 (31%), Positives = 18/45 (40%) Frame = +3 Query: 309 VVTSYPRM*TRPSLPSKPSVLSNSSTGVQPVSRSVSTTSHPPWCP 443 VV P + RP++P P S PV V + PP P Sbjct: 642 VVPEVPSVPQRPAVPVVPEAPSVPQPPAAPVVPEVPSVPQPPAVP 686 >SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||Manual Length = 534 Score = 29.1 bits (62), Expect = 0.55 Identities = 23/81 (28%), Positives = 41/81 (50%) Frame = +3 Query: 144 STSHWSRTRQSSLPRRPTMNSFPSPRSQTHASSPPTRW*NATPVMASTWLAVCCTVVTSY 323 STS + T SS RP +S S +H SS ++ ++ PV ++ + + +S+ Sbjct: 377 STSSSTLTSSSSSSSRPASSSSHSSSLSSHKSSSSSKS-SSAPVSSAFYHNSTSSRSSSH 435 Query: 324 PRM*TRPSLPSKPSVLSNSST 386 + SL SKP + ++SS+ Sbjct: 436 SSSHSLSSLSSKPILTASSSS 456 >SPAC19D5.04 |ptr1||HECT domain|Schizosaccharomyces pombe|chr 1|||Manual Length = 3227 Score = 28.7 bits (61), Expect = 0.72 Identities = 20/73 (27%), Positives = 34/73 (46%) Frame = -1 Query: 349 SDGRVHILGYDVTTVQHTASHVLAMTGVAFHHLVGGLEACVCDLGDGKLFMVGLLGRDDW 170 S G +LGY ++ A++V+A + V HL+ G D + K G D Sbjct: 407 SAGLTSLLGYHLSVKTPQATYVVARSIVMLDHLIDGYSMAFPDFSESK-------GLDML 459 Query: 169 RVRDQWEVDTGVR 131 R Q+E++ G++ Sbjct: 460 VDRVQYELEAGLQ 472 >SPCC18.03 |||shuttle craft like transcriptional regulator|Schizosaccharomyces pombe|chr 3|||Manual Length = 1077 Score = 28.3 bits (60), Expect = 0.95 Identities = 14/42 (33%), Positives = 18/42 (42%) Frame = +2 Query: 221 ITNACFEPANQMVKCDPRHGKYMACCMLYRGDVVPKDVNAAI 346 + ACFEP N C H K C L + KD N ++ Sbjct: 361 LCGACFEPINAKCYCG-LHSKTYPCSSLPSPSISKKDENGSV 401 >SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual Length = 1611 Score = 27.9 bits (59), Expect = 1.3 Identities = 17/49 (34%), Positives = 26/49 (53%) Frame = +3 Query: 108 PSSRLTWCLTPVSTSHWSRTRQSSLPRRPTMNSFPSPRSQTHASSPPTR 254 P+++ P S + T +SS+P P N+ PSP S + AS+ P R Sbjct: 1232 PTAKAPPVPAPSSEAPSVSTPRSSVPS-PHSNASPSPTSSSMASAAPAR 1279 >SPAC1006.06 |rgf2||RhoGEF Rgf2|Schizosaccharomyces pombe|chr 1|||Manual Length = 1158 Score = 26.2 bits (55), Expect = 3.8 Identities = 11/28 (39%), Positives = 17/28 (60%) Frame = +3 Query: 171 QSSLPRRPTMNSFPSPRSQTHASSPPTR 254 + SLPRRP+ +P S T ++ PP + Sbjct: 738 RGSLPRRPSSALLTNPISITKSNPPPVK 765 >SPAC1B3.15c |||membrane transporter|Schizosaccharomyces pombe|chr 1|||Manual Length = 628 Score = 26.2 bits (55), Expect = 3.8 Identities = 14/32 (43%), Positives = 21/32 (65%), Gaps = 3/32 (9%) Frame = +3 Query: 345 SLPSKPSV---LSNSSTGVQPVSRSVSTTSHP 431 +LP KPS+ +++S V+P S STTS+P Sbjct: 5 TLPPKPSISPSIASSFPTVKPFSSQNSTTSNP 36 >SPBC428.10 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||Manual Length = 751 Score = 25.8 bits (54), Expect = 5.1 Identities = 16/47 (34%), Positives = 26/47 (55%) Frame = +3 Query: 105 SPSSRLTWCLTPVSTSHWSRTRQSSLPRRPTMNSFPSPRSQTHASSP 245 SPS LT L S++ S + + + R+ T +S SP+ Q+ S+P Sbjct: 619 SPSKMLT-TLRNNSSTFPSLRKNAMIARKSTADSLSSPKRQSVPSTP 664 >SPAC30D11.13 |hus5|ubc9|SUMO conjugating enzyme Hus5|Schizosaccharomyces pombe|chr 1|||Manual Length = 157 Score = 25.8 bits (54), Expect = 5.1 Identities = 10/20 (50%), Positives = 11/20 (55%) Frame = -3 Query: 326 WVRRHHGTAYSKPCTCHDGG 267 W R H Y+KPC DGG Sbjct: 16 WRRDHPFGFYAKPCKSSDGG 35 >SPAC890.02c |alp7|mia1|TACC homolog |Schizosaccharomyces pombe|chr 1|||Manual Length = 474 Score = 25.8 bits (54), Expect = 5.1 Identities = 16/51 (31%), Positives = 31/51 (60%) Frame = +3 Query: 132 LTPVSTSHWSRTRQSSLPRRPTMNSFPSPRSQTHASSPPTRW*NATPVMAS 284 LTP + S +S + +S +R ++N+ PS + +H+SS T + PV+++ Sbjct: 130 LTPKNPSLFSSSNAAS--QRGSLNTAPSNFAYSHSSSLQTSASSRPPVLSN 178 >SPAC222.10c |byr4||two-component GAP Byr4|Schizosaccharomyces pombe|chr 1|||Manual Length = 665 Score = 25.4 bits (53), Expect = 6.7 Identities = 13/34 (38%), Positives = 19/34 (55%), Gaps = 2/34 (5%) Frame = +3 Query: 342 PSLPSKPSVLSNSST--GVQPVSRSVSTTSHPPW 437 P PS+P+++SN ST G+Q V V + W Sbjct: 531 PISPSRPALISNISTKKGIQVVGNMVYDPTRLRW 564 >SPAC23E2.02 |lsd2|swm2, saf140|histone demethylase SWIRM2 |Schizosaccharomyces pombe|chr 1|||Manual Length = 1273 Score = 25.4 bits (53), Expect = 6.7 Identities = 22/84 (26%), Positives = 36/84 (42%) Frame = +3 Query: 138 PVSTSHWSRTRQSSLPRRPTMNSFPSPRSQTHASSPPTRW*NATPVMASTWLAVCCTVVT 317 P+ SH S + S+ R + + H S PT+ +AT + S AV T Sbjct: 253 PLDASHLSSQQLLSMYRDQVSHGVTPSTFRNHESFMPTQLVSATELSKSVDNAVLPIPPT 312 Query: 318 SYPRM*TRPSLPSKPSVLSNSSTG 389 + P + P S P + S +++G Sbjct: 313 TAPAV-VSPPASSFPLMSSAATSG 335 >SPBC3B8.10c |||NLI interacting factor family|Schizosaccharomyces pombe|chr 2|||Manual Length = 476 Score = 25.0 bits (52), Expect = 8.9 Identities = 15/37 (40%), Positives = 16/37 (43%) Frame = +3 Query: 324 PRM*TRPSLPSKPSVLSNSSTGVQPVSRSVSTTSHPP 434 PR LP K VL T + VSR TTS P Sbjct: 293 PRPLLNSKLPRKTLVLDLDETLIHSVSRGSRTTSGQP 329 >SPAC13G6.10c |||O-glucosyl hydrolase |Schizosaccharomyces pombe|chr 1|||Manual Length = 530 Score = 25.0 bits (52), Expect = 8.9 Identities = 19/60 (31%), Positives = 26/60 (43%) Frame = +3 Query: 264 ATPVMASTWLAVCCTVVTSYPRM*TRPSLPSKPSVLSNSSTGVQPVSRSVSTTSHPPWCP 443 +TP ++S A VV+S T + S S ++SS P S SVS W P Sbjct: 245 STPAVSSA-AASSSAVVSSVVSSATSVAASSTISSATSSSASASPTSSSVSGKRGLAWIP 303 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,611,684 Number of Sequences: 5004 Number of extensions: 55191 Number of successful extensions: 238 Number of sequences better than 10.0: 19 Number of HSP's better than 10.0 without gapping: 214 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 236 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 275671126 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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