Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= brP-0123
(723 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At2g44350.2 68415.m05517 citrate synthase, mitochondrial, putati... 237 5e-63
At2g44350.1 68415.m05516 citrate synthase, mitochondrial, putati... 237 5e-63
At3g60100.1 68416.m06711 citrate synthase, mitochondrial, putati... 224 5e-59
At3g58740.1 68416.m06547 citrate synthase, glyoxysomal, putative... 47 1e-05
At2g42790.1 68415.m05298 citrate synthase, glyoxysomal, putative... 45 4e-05
At3g58750.1 68416.m06548 citrate synthase, glyoxysomal, putative... 44 1e-04
At2g11270.1 68415.m01208 citrate synthase-related contains simil... 44 1e-04
At1g59650.1 68414.m06709 expressed protein ; supporting cDNA gi|... 30 1.4
At5g53030.2 68418.m06586 expressed protein 29 3.1
At5g53030.1 68418.m06587 expressed protein 29 3.1
At2g22680.1 68415.m02688 zinc finger (C3HC4-type RING finger) fa... 29 4.1
At5g56710.1 68418.m07078 60S ribosomal protein L31 (RPL31C) 28 5.5
At4g21300.1 68417.m03077 pentatricopeptide (PPR) repeat-containi... 28 5.5
At4g16442.1 68417.m02489 integral membrane family protein contai... 28 5.5
At3g52890.2 68416.m05829 protein kinase (KIPK) identical to prot... 28 7.2
At3g52890.1 68416.m05828 protein kinase (KIPK) identical to prot... 28 7.2
>At2g44350.2 68415.m05517 citrate synthase, mitochondrial, putative
strong similarity to SP|P20115 Citrate synthase,
mitochondrial precursor {Arabidopsis thaliana};
contains Pfam profile PF00285: Citrate synthase
Length = 474
Score = 237 bits (580), Expect = 5e-63
Identities = 100/186 (53%), Positives = 143/186 (76%)
Frame = +1
Query: 166 PRXQEKIREFRKKHGSTKVGEVTVDMMYGGMRGIKGLVWETSVLDADEGIRFRGLSIPEC 345
P Q+++++ + +HG ++G +TVDM+ GGMRG+ GL+WETS+LD +EGIRFRGLSIPEC
Sbjct: 50 PEQQDRLKKLKSEHGKVQLGNITVDMVIGGMRGMTGLLWETSLLDPEEGIRFRGLSIPEC 109
Query: 346 QQQLPKAKGGEEPLPEGLFWLLVTGDIPTEAQAKALSKEWAARAELPAHVVTMLNNMPGK 525
Q+ LP A+ G EPLPEGL WLL+TG +P++ Q +ALSK+ A RA +P +V ++ +P
Sbjct: 110 QKVLPTAQSGAEPLPEGLLWLLLTGKVPSKEQVEALSKDLANRAAVPDYVYNAIDALPST 169
Query: 526 LHPMSQFSAAVTALNSESKFAKAYSXGVHKSKYWEYVYEDTMNLIAKLPVIAATIYRNVY 705
HPM+QF++ V AL +S+F KAY G+HKSK+WE YED +NLIA++PV+AA +YR +Y
Sbjct: 170 AHPMTQFASGVMALQVQSEFQKAYENGIHKSKFWEPTYEDCLNLIARVPVVAAYVYRRMY 229
Query: 706 RDGKGI 723
++G I
Sbjct: 230 KNGDSI 235
>At2g44350.1 68415.m05516 citrate synthase, mitochondrial, putative
strong similarity to SP|P20115 Citrate synthase,
mitochondrial precursor {Arabidopsis thaliana};
contains Pfam profile PF00285: Citrate synthase
Length = 473
Score = 237 bits (580), Expect = 5e-63
Identities = 100/186 (53%), Positives = 143/186 (76%)
Frame = +1
Query: 166 PRXQEKIREFRKKHGSTKVGEVTVDMMYGGMRGIKGLVWETSVLDADEGIRFRGLSIPEC 345
P Q+++++ + +HG ++G +TVDM+ GGMRG+ GL+WETS+LD +EGIRFRGLSIPEC
Sbjct: 49 PEQQDRLKKLKSEHGKVQLGNITVDMVIGGMRGMTGLLWETSLLDPEEGIRFRGLSIPEC 108
Query: 346 QQQLPKAKGGEEPLPEGLFWLLVTGDIPTEAQAKALSKEWAARAELPAHVVTMLNNMPGK 525
Q+ LP A+ G EPLPEGL WLL+TG +P++ Q +ALSK+ A RA +P +V ++ +P
Sbjct: 109 QKVLPTAQSGAEPLPEGLLWLLLTGKVPSKEQVEALSKDLANRAAVPDYVYNAIDALPST 168
Query: 526 LHPMSQFSAAVTALNSESKFAKAYSXGVHKSKYWEYVYEDTMNLIAKLPVIAATIYRNVY 705
HPM+QF++ V AL +S+F KAY G+HKSK+WE YED +NLIA++PV+AA +YR +Y
Sbjct: 169 AHPMTQFASGVMALQVQSEFQKAYENGIHKSKFWEPTYEDCLNLIARVPVVAAYVYRRMY 228
Query: 706 RDGKGI 723
++G I
Sbjct: 229 KNGDSI 234
>At3g60100.1 68416.m06711 citrate synthase, mitochondrial, putative
strong similarity to SP|Q43175 Citrate synthase,
mitochondrial precursor {Solanum tuberosum}; contains
Pfam profile PF00285: Citrate synthase
Length = 433
Score = 224 bits (547), Expect = 5e-59
Identities = 101/184 (54%), Positives = 138/184 (75%), Gaps = 1/184 (0%)
Frame = +1
Query: 166 PRXQEKIREFRKKHGSTKVGEVTVDMMYGGMRGIKGLVWETSVLDADEGIRFRGLSIPEC 345
P Q+++++ + + G VG +TVDM+ GGMRG+ GL+WETS+LDADEGIRFRG+SIPEC
Sbjct: 15 PEQQDRLKKLKSEQGKVPVGNITVDMVLGGMRGMTGLLWETSLLDADEGIRFRGMSIPEC 74
Query: 346 QQQLPKAKGGEEPLPEGLFWLLVTGDIPTEAQAKALSKEWAARAELPAHVVTMLNNMPGK 525
Q+ LP A+ GEEPLPE L WLL+TG +PT+ QA ALS E A RA +PA ++ +P
Sbjct: 75 QKILPSAESGEEPLPESLLWLLLTGKVPTKEQANALSTELAHRAAVPA-----IDALPST 129
Query: 526 LHPMSQFSAAVTALNSESKFAKAYSXG-VHKSKYWEYVYEDTMNLIAKLPVIAATIYRNV 702
HPM+QF++ V AL +S+F KAY G + KSKYWE +ED +NLIA++PV+A+ +YR +
Sbjct: 130 AHPMTQFASGVMALQVQSEFQKAYEQGDISKSKYWEPTFEDALNLIARVPVVASYVYRRM 189
Query: 703 YRDG 714
Y+DG
Sbjct: 190 YKDG 193
>At3g58740.1 68416.m06547 citrate synthase, glyoxysomal, putative
strong similarity to SP|P49299 Citrate synthase,
glyoxysomal precursor {Cucurbita maxima}; contains Pfam
profile PF00285: Citrate synthase
Length = 480
Score = 46.8 bits (106), Expect = 1e-05
Identities = 36/138 (26%), Positives = 67/138 (48%), Gaps = 3/138 (2%)
Frame = +1
Query: 289 SVLDADEGI-RFRGLSIPECQQQLPKAKGGEEPLPEGLFWLLVTGDIPTEAQAKALSKEW 465
S +D DEGI R+RG + E ++ + + +LL+ G++P++ Q
Sbjct: 107 SYIDGDEGILRYRGYPVEELAEKSTYTE---------VTYLLIYGNLPSQRQLADWEFAI 157
Query: 466 AARAELPAHVVTMLNNMPGKLHPMSQFSAAVTALNSESKFA--KAYSXGVHKSKYWEYVY 639
+ + +P V+ M+ +MP +HP+ A++AL+ A GV+KSK +
Sbjct: 158 SQNSAVPQGVLDMIQSMPNDVHPVGALVTAMSALSIFYPDANPSLMGLGVYKSK--QVRD 215
Query: 640 EDTMNLIAKLPVIAATIY 693
+ + ++ + P IAA Y
Sbjct: 216 KQIVRVLGQAPTIAAAAY 233
>At2g42790.1 68415.m05298 citrate synthase, glyoxysomal, putative
strong similarity to SP|P49299 Citrate synthase,
glyoxysomal precursor {Cucurbita maxima}; contains Pfam
profile PF00285: Citrate synthase
Length = 509
Score = 45.2 bits (102), Expect = 4e-05
Identities = 38/138 (27%), Positives = 66/138 (47%), Gaps = 3/138 (2%)
Frame = +1
Query: 289 SVLDADEGI-RFRGLSIPECQQQLPKAKGGEEPLPEGLFWLLVTGDIPTEAQAKALSKEW 465
S +D DEGI R+RG I E + + + +LL+ G++P+E+Q
Sbjct: 105 SYIDGDEGILRYRGYPIEEMAENSTFLE---------VAYLLMYGNLPSESQLSDWEFAV 155
Query: 466 AARAELPAHVVTMLNNMPGKLHPMSQFSAAVTALNSESKFAKAYSXG--VHKSKYWEYVY 639
+ + +P V+ ++ +MP HPM +A++AL+ A G ++ SK +
Sbjct: 156 SQHSAVPQGVLDIIQSMPHDAHPMGVLVSAMSALSIFHPDANPALRGQDIYDSK--QVRD 213
Query: 640 EDTMNLIAKLPVIAATIY 693
+ + +I K P IAA Y
Sbjct: 214 KQIIRIIGKAPTIAAAAY 231
>At3g58750.1 68416.m06548 citrate synthase, glyoxysomal, putative
strong similarity to SP|P49299 Citrate synthase,
glyoxysomal precursor {Cucurbita maxima}; contains Pfam
profile PF00285: Citrate synthase
Length = 514
Score = 44.0 bits (99), Expect = 1e-04
Identities = 36/136 (26%), Positives = 66/136 (48%), Gaps = 3/136 (2%)
Frame = +1
Query: 295 LDADEGI-RFRGLSIPECQQQLPKAKGGEEPLPEGLFWLLVTGDIPTEAQAKALSKEWAA 471
+D DEGI R+RG I E + + + +LL+ G++P+++Q +
Sbjct: 112 IDGDEGILRYRGYPIEELAESSTFIE---------VAYLLMYGNLPSQSQLADWEFTVSQ 162
Query: 472 RAELPAHVVTMLNNMPGKLHPMSQFSAAVTALNSESKFAKAYSXG--VHKSKYWEYVYED 645
+ +P V+ ++ +MP HPM +A++AL+ A G ++KSK + +
Sbjct: 163 HSAVPQGVLDIIQSMPHDAHPMGVLVSAMSALSIFHPDANPALSGQDIYKSK--QVRDKQ 220
Query: 646 TMNLIAKLPVIAATIY 693
+ ++ K P IAA Y
Sbjct: 221 IVRILGKAPTIAAAAY 236
>At2g11270.1 68415.m01208 citrate synthase-related contains
similarity to Swiss-Prot:P20115 citrate synthase,
mitochondrial precursor [Arabidopsis thaliana]
Length = 83
Score = 44.0 bits (99), Expect = 1e-04
Identities = 17/34 (50%), Positives = 25/34 (73%)
Frame = +1
Query: 175 QEKIREFRKKHGSTKVGEVTVDMMYGGMRGIKGL 276
Q++ ++ + KHG VG +TVDM+ GGMRG+ GL
Sbjct: 43 QDRSKKLKLKHGKVPVGNITVDMVLGGMRGMTGL 76
>At1g59650.1 68414.m06709 expressed protein ; supporting cDNA
gi|6520232|dbj|AB028233.1|
Length = 492
Score = 30.3 bits (65), Expect = 1.4
Identities = 12/29 (41%), Positives = 19/29 (65%)
Frame = +2
Query: 512 ICPANCIPCHSSRLPSPHSTVNLNSLKPT 598
I P+NC+PC +S +PS +L+S P+
Sbjct: 157 ILPSNCLPCLNSTVPSIEKRRSLSSSPPS 185
>At5g53030.2 68418.m06586 expressed protein
Length = 224
Score = 29.1 bits (62), Expect = 3.1
Identities = 14/36 (38%), Positives = 19/36 (52%)
Frame = +1
Query: 286 TSVLDADEGIRFRGLSIPECQQQLPKAKGGEEPLPE 393
T+VLD +R R LS+P + K +G P PE
Sbjct: 98 TTVLDGPYDLRRRSLSLPRSAAVIRKLRGVPAPAPE 133
>At5g53030.1 68418.m06587 expressed protein
Length = 245
Score = 29.1 bits (62), Expect = 3.1
Identities = 14/36 (38%), Positives = 19/36 (52%)
Frame = +1
Query: 286 TSVLDADEGIRFRGLSIPECQQQLPKAKGGEEPLPE 393
T+VLD +R R LS+P + K +G P PE
Sbjct: 98 TTVLDGPYDLRRRSLSLPRSAAVIRKLRGVPAPAPE 133
>At2g22680.1 68415.m02688 zinc finger (C3HC4-type RING finger)
family protein contains Pfam profiles PF00097: Zinc
finger, C3HC4 type (RING finger), PF00092: von
Willebrand factor type A domain
Length = 683
Score = 28.7 bits (61), Expect = 4.1
Identities = 14/53 (26%), Positives = 25/53 (47%)
Frame = +3
Query: 51 ITSSRLVELQKACPTATVLLRGLSAEQTNLKSILQEKIPKXTREDPRIPKEAW 209
+TS+R + +Q ++ +RGL AE +L S+ + + P AW
Sbjct: 601 LTSARALLVQHGLSSSDACIRGLDAEIADLNSVKGRHVAASESLESLTPTSAW 653
>At5g56710.1 68418.m07078 60S ribosomal protein L31 (RPL31C)
Length = 119
Score = 28.3 bits (60), Expect = 5.5
Identities = 26/104 (25%), Positives = 46/104 (44%)
Frame = +1
Query: 124 LNRRI*KVFSRKRFPRXQEKIREFRKKHGSTKVGEVTVDMMYGGMRGIKGLVWETSVLDA 303
L+RR+ K +K+ P+ ++IR+F +K TK +V VD+ + +W +
Sbjct: 20 LHRRLHKCTFKKKAPKAIKEIRKFAEKAMGTK--DVRVDVK------LNKQIWSKGIRGP 71
Query: 304 DEGIRFRGLSIPECQQQLPKAKGGEEPLPEGLFWLLVTGDIPTE 435
IR R + + + +E E F L+ +IP E
Sbjct: 72 PRRIRVR----------VARKRNDDEDAKEEFFSLVTVAEIPAE 105
>At4g21300.1 68417.m03077 pentatricopeptide (PPR) repeat-containing
protein contains INTERPRO:IPR002885 PPR repeats
Length = 857
Score = 28.3 bits (60), Expect = 5.5
Identities = 16/64 (25%), Positives = 30/64 (46%)
Frame = +3
Query: 42 LFRITSSRLVELQKACPTATVLLRGLSAEQTNLKSILQEKIPKXTREDPRIPKEAWFH*S 221
L + SS+L++L + VL+ A +S+ + + RE +IP +W +
Sbjct: 730 LAEVASSKLMDLDPSNSGYYVLISNAHANAREWESVTKVRSLMKEREVQKIPGYSWIEIN 789
Query: 222 RRGH 233
+R H
Sbjct: 790 KRTH 793
>At4g16442.1 68417.m02489 integral membrane family protein contains
TIGRFAM TIGR01569 : plant integral membrane protein
TIGR01569; contains Pfam PF04535 : Domain of unknown
function (DUF588)
Length = 182
Score = 28.3 bits (60), Expect = 5.5
Identities = 12/38 (31%), Positives = 20/38 (52%)
Frame = +2
Query: 233 QLI*CTVACVESKVWFGKPLCWMPMKESVSVVYPSLSA 346
Q + C V ++ KV F KPL W ++ Y +++A
Sbjct: 71 QSVRCVVGTMKGKVLFSKPLAWAFFSGDQAMAYLNVAA 108
>At3g52890.2 68416.m05829 protein kinase (KIPK) identical to protein
kinase KIPK (KCBP-interacting protein kinase)
[Arabidopsis thaliana] gi|7716430|gb|AAF68383
Length = 934
Score = 27.9 bits (59), Expect = 7.2
Identities = 17/42 (40%), Positives = 21/42 (50%), Gaps = 4/42 (9%)
Frame = +1
Query: 322 RGLSIPECQQQLPKAKGGEE----PLPEGLFWLLVTGDIPTE 435
RGL + E + +L KG E P EGL W L+ IP E
Sbjct: 853 RGLLVKEPENRLGSEKGSVEIKRHPFFEGLNWALIRCAIPPE 894
>At3g52890.1 68416.m05828 protein kinase (KIPK) identical to protein
kinase KIPK (KCBP-interacting protein kinase)
[Arabidopsis thaliana] gi|7716430|gb|AAF68383
Length = 934
Score = 27.9 bits (59), Expect = 7.2
Identities = 17/42 (40%), Positives = 21/42 (50%), Gaps = 4/42 (9%)
Frame = +1
Query: 322 RGLSIPECQQQLPKAKGGEE----PLPEGLFWLLVTGDIPTE 435
RGL + E + +L KG E P EGL W L+ IP E
Sbjct: 853 RGLLVKEPENRLGSEKGSVEIKRHPFFEGLNWALIRCAIPPE 894
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 15,974,427
Number of Sequences: 28952
Number of extensions: 330690
Number of successful extensions: 1083
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 1042
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1081
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1575119672
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
- SilkBase 1999-2023 -