BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= brP-0116 (848 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPCC1739.13 |ssa2||heat shock protein Ssa2|Schizosaccharomyces p... 274 1e-74 SPAC13G7.02c |ssa1||heat shock protein Ssa1|Schizosaccharomyces ... 267 2e-72 SPBC1709.05 |sks2|hsc1|heat shock protein Sks2|Schizosaccharomyc... 238 8e-64 SPAC22A12.15c |bip1|bip|BiP |Schizosaccharomyces pombe|chr 1|||M... 227 2e-60 SPAC664.11 |ssc1|ssp1|Hsp70 chaperone mtHsp70|Schizosaccharomyce... 188 1e-48 SPAC110.04c |pss1|ssp1, SPAP14E8.01c|heat shock protein Pss1|Sch... 92 1e-19 SPAC57A7.12 |||heat shock protein Pdr13 |Schizosaccharomyces pom... 69 1e-12 SPAC1F5.06 |||heat shock protein Lhs1 |Schizosaccharomyces pombe... 51 2e-07 SPAC11H11.01 |sst6|cps23|ESCRT I complex subunit Vps23|Schizosac... 27 3.4 SPAC19G12.10c |cpy1|pcy1|vacuolar carboxypeptidase Y|Schizosacch... 27 4.4 SPBC17G9.03c |||lysine-tRNA ligase|Schizosaccharomyces pombe|chr... 26 7.8 >SPCC1739.13 |ssa2||heat shock protein Ssa2|Schizosaccharomyces pombe|chr 3|||Manual Length = 647 Score = 274 bits (672), Expect = 1e-74 Identities = 146/246 (59%), Positives = 170/246 (69%) Frame = +3 Query: 81 IDLGTTYSCVGVFQHG*GGDHRQRPGXTGTTSVLCCVHXTPSVSSGDAAKNQVAMNPNNT 260 IDLGTTYSCVG F + G T S + + GDAAKNQVAMNP+NT Sbjct: 7 IDLGTTYSCVGHFSNNRVEIIANDQGNRTTPSYVAFTDTERLI--GDAAKNQVAMNPHNT 64 Query: 261 IFDAKRLIGRKFEDATVQADMKHWPFEVVSDGGKPKIKVAYKGEDKTFFPEEVSSMVLTK 440 IFDAKRLIGRKF+D VQ+DMKHWPF+V+S GKP ++V YKGE KTF PEE+SSMVL K Sbjct: 65 IFDAKRLIGRKFDDPEVQSDMKHWPFKVISKDGKPVLQVEYKGETKTFTPEEISSMVLMK 124 Query: 441 MKETAEAYLGKTVQNAVITVPAYFNDSQRQATKDAGTISGLNVLRIINEPTAAAIAYGLD 620 M+ETAEAYLG V +AV+TVPAYFNDSQRQATKDAG I+GLNVLRIINEPTAAAIAYGLD Sbjct: 125 MRETAEAYLGGKVTDAVVTVPAYFNDSQRQATKDAGLIAGLNVLRIINEPTAAAIAYGLD 184 Query: 621 KKGYWRTKCTYL*PRRRYLSTCPSLPSKMVSFEVKSTXGDTHFGKXXTLTIRMVNHFVQE 800 + + + L + FEVK+T GDTH G R+VNHF+QE Sbjct: 185 RSNQGESN-VLIFDLGGGTFDVSLLTIEEGIFEVKATAGDTHLG-GEDFDSRLVNHFIQE 242 Query: 801 FRRNSK 818 F+R +K Sbjct: 243 FKRKNK 248 Score = 33.9 bits (74), Expect = 0.029 Identities = 14/19 (73%), Positives = 15/19 (78%) Frame = +1 Query: 622 KKGTGERNVLIFDLGGGTF 678 + GE NVLIFDLGGGTF Sbjct: 185 RSNQGESNVLIFDLGGGTF 203 Score = 27.5 bits (58), Expect = 2.5 Identities = 11/13 (84%), Positives = 13/13 (100%) Frame = +2 Query: 677 FDVSILTIEDGIF 715 FDVS+LTIE+GIF Sbjct: 203 FDVSLLTIEEGIF 215 >SPAC13G7.02c |ssa1||heat shock protein Ssa1|Schizosaccharomyces pombe|chr 1|||Manual Length = 644 Score = 267 bits (654), Expect = 2e-72 Identities = 143/246 (58%), Positives = 167/246 (67%) Frame = +3 Query: 81 IDLGTTYSCVGVFQHG*GGDHRQRPGXTGTTSVLCCVHXTPSVSSGDAAKNQVAMNPNNT 260 IDLGTTYSCVG F + G T S + + GDAAKNQVAMNP+NT Sbjct: 7 IDLGTTYSCVGHFSNNRVEIIANDQGNRTTPSYVAFTDTERLI--GDAAKNQVAMNPHNT 64 Query: 261 IFDAKRLIGRKFEDATVQADMKHWPFEVVSDGGKPKIKVAYKGEDKTFFPEEVSSMVLTK 440 IFDAKRLIGR+F D VQ+DMKHWPF+V+ GKP I+V +KGE KTF PEE+SSMVL K Sbjct: 65 IFDAKRLIGRRFNDPEVQSDMKHWPFKVIEKDGKPLIQVEFKGETKTFTPEEISSMVLLK 124 Query: 441 MKETAEAYLGKTVQNAVITVPAYFNDSQRQATKDAGTISGLNVLRIINEPTAAAIAYGLD 620 M+E+AEA+LG V +AV+TVPAYFNDSQRQATKDAG I+GLNVLRIINEPTAAAIAYGLD Sbjct: 125 MRESAEAFLGGKVTDAVVTVPAYFNDSQRQATKDAGLIAGLNVLRIINEPTAAAIAYGLD 184 Query: 621 KKGYWRTKCTYL*PRRRYLSTCPSLPSKMVSFEVKSTXGDTHFGKXXTLTIRMVNHFVQE 800 + T + L + FEVK+T GDTH G R+VNHF QE Sbjct: 185 RSNQHETN-VLIFDLGGGTFDVSLLTIEEGIFEVKATAGDTHLG-GEDFDSRLVNHFAQE 242 Query: 801 FRRNSK 818 F+R +K Sbjct: 243 FKRKNK 248 >SPBC1709.05 |sks2|hsc1|heat shock protein Sks2|Schizosaccharomyces pombe|chr 2|||Manual Length = 613 Score = 238 bits (582), Expect = 8e-64 Identities = 132/246 (53%), Positives = 157/246 (63%) Frame = +3 Query: 81 IDLGTTYSCVGVFQHG*GGDHRQRPGXTGTTSVLCCVHXTPSVSSGDAAKNQVAMNPNNT 260 IDLGTTYSCV V++ G T S + V GDAAKNQ AMNP NT Sbjct: 11 IDLGTTYSCVAVWETANVEIIPNDQGARTTPSFVAFTETERLV--GDAAKNQAAMNPRNT 68 Query: 261 IFDAKRLIGRKFEDATVQADMKHWPFEVVSDGGKPKIKVAYKGEDKTFFPEEVSSMVLTK 440 +FDAKRLIGR++ED Q D+KHWPF+V+ + G P I+V Y GE K F +E+S+MVLTK Sbjct: 69 VFDAKRLIGRRYEDPETQKDIKHWPFKVIDNNGIPTIEVNYLGEKKQFTAQEISAMVLTK 128 Query: 441 MKETAEAYLGKTVQNAVITVPAYFNDSQRQATKDAGTISGLNVLRIINEPTAAAIAYGLD 620 MKE +EA L K V+ AVITVPAYF+DSQR ATKDAG I+GLNVLRIINEPTAAAIAYGLD Sbjct: 129 MKEISEAKLNKRVEKAVITVPAYFSDSQRAATKDAGAIAGLNVLRIINEPTAAAIAYGLD 188 Query: 621 KKGYWRTKCTYL*PRRRYLSTCPSLPSKMVSFEVKSTXGDTHFGKXXTLTIRMVNHFVQE 800 K + K + L + FEV +T GDTH G +V HF+QE Sbjct: 189 AKSD-KPKNVLIFDLGGGTFDVSLLKIQGGVFEVLATAGDTHLG-GEDFDNALVEHFIQE 246 Query: 801 FRRNSK 818 F+R K Sbjct: 247 FKRKQK 252 Score = 30.7 bits (66), Expect = 0.27 Identities = 13/18 (72%), Positives = 14/18 (77%) Frame = +1 Query: 625 KGTGERNVLIFDLGGGTF 678 K +NVLIFDLGGGTF Sbjct: 190 KSDKPKNVLIFDLGGGTF 207 >SPAC22A12.15c |bip1|bip|BiP |Schizosaccharomyces pombe|chr 1|||Manual Length = 663 Score = 227 bits (554), Expect = 2e-60 Identities = 122/245 (49%), Positives = 161/245 (65%) Frame = +3 Query: 81 IDLGTTYSCVGVFQHG*GGDHRQRPGXTGTTSVLCCVHXTPSVSSGDAAKNQVAMNPNNT 260 IDLGTTYSCV V ++G G T S + V G+AAKNQ NP NT Sbjct: 40 IDLGTTYSCVAVMKNGRVEIIANDQGNRITPSYVAFTEDERLV--GEAAKNQAPSNPENT 97 Query: 261 IFDAKRLIGRKFEDATVQADMKHWPFEVVSDGGKPKIKVAYKGEDKTFFPEEVSSMVLTK 440 IFD KRLIGRKF++ T+ D+K +PF +V+D +P ++V G+ K F PEE+S+M+L+K Sbjct: 98 IFDIKRLIGRKFDEKTMAKDIKSFPFHIVNDKNRPLVEVNVGGKKKKFTPEEISAMILSK 157 Query: 441 MKETAEAYLGKTVQNAVITVPAYFNDSQRQATKDAGTISGLNVLRIINEPTAAAIAYGLD 620 MK+TAEAYLGK V +AV+TVPAYFND+QRQATKDAGTI+GLNV+RI+NEPTAAAIAYGLD Sbjct: 158 MKQTAEAYLGKPVTHAVVTVPAYFNDAQRQATKDAGTIAGLNVIRIVNEPTAAAIAYGLD 217 Query: 621 KKGYWRTKCTYL*PRRRYLSTCPSLPSKMVSFEVKSTXGDTHFGKXXTLTIRMVNHFVQE 800 K + Y + + S+ + + FEV +T GDTH G R++N+ + Sbjct: 218 KTDTEKHIVVYDLGGGTFDVSLLSIDNGV--FEVLATSGDTHLG-GEDFDNRVINYLART 274 Query: 801 FRRNS 815 + R + Sbjct: 275 YNRKN 279 >SPAC664.11 |ssc1|ssp1|Hsp70 chaperone mtHsp70|Schizosaccharomyces pombe|chr 1|||Manual Length = 674 Score = 188 bits (457), Expect = 1e-48 Identities = 109/250 (43%), Positives = 143/250 (57%) Frame = +3 Query: 63 KHPQ*AIDLGTTYSCVGVFQHG*GGDHRQRPGXTGTTSVLCCVHXTPSVSSGDAAKNQVA 242 K P IDLGTT SC+ + + G T SV+ + G +AK Q Sbjct: 49 KGPVIGIDLGTTTSCLAIMEGQTPKVIANAEGTRTTPSVVAFTKDGERLV-GVSAKRQAV 107 Query: 243 MNPNNTIFDAKRLIGRKFEDATVQADMKHWPFEVVSDGGKPKIKVAYKGEDKTFFPEEVS 422 +NP NT F KRLIGR+F++ VQ D+K P+++V + KT+ P ++ Sbjct: 108 INPENTFFATKRLIGRRFKEPEVQRDIKEVPYKIVEHSNGD---AWLEARGKTYSPSQIG 164 Query: 423 SMVLTKMKETAEAYLGKTVQNAVITVPAYFNDSQRQATKDAGTISGLNVLRIINEPTAAA 602 +L+KM+ETA YLGK V+NAV+TVPAYFNDSQRQATK AG I+GLNVLR++NEPTAAA Sbjct: 165 GFILSKMRETASTYLGKDVKNAVVTVPAYFNDSQRQATKAAGAIAGLNVLRVVNEPTAAA 224 Query: 603 IAYGLDKKGYWRTKCTYL*PRRRYLSTCPSLPSKMVSFEVKSTXGDTHFGKXXTLTIRMV 782 +AYGLDKK L +S L FEV+ST GDTH G + +V Sbjct: 225 LAYGLDKKNDAIVAVFDLGGGTFDISI---LELNNGVFEVRSTNGDTHLG-GEDFDVALV 280 Query: 783 NHFVQEFRRN 812 H V+ F++N Sbjct: 281 RHIVETFKKN 290 >SPAC110.04c |pss1|ssp1, SPAP14E8.01c|heat shock protein Pss1|Schizosaccharomyces pombe|chr 1|||Manual Length = 720 Score = 91.9 bits (218), Expect = 1e-19 Identities = 51/137 (37%), Positives = 79/137 (57%), Gaps = 1/137 (0%) Frame = +3 Query: 216 GDAAKNQVAMNPNNTIFDAKRLIGRKFEDATVQ-ADMKHWPFEVVSDGGKPKIKVAYKGE 392 G+AAK+ A N NT+ KRL GR ++D ++ + ++ G KV Y E Sbjct: 53 GEAAKSAEASNFRNTVGSLKRLAGRTYDDPEIKDIESNFISAKLTEVDGFVGAKVQYLNE 112 Query: 393 DKTFFPEEVSSMVLTKMKETAEAYLGKTVQNAVITVPAYFNDSQRQATKDAGTISGLNVL 572 + F ++ + TK+K AEA L +V + VI++PA+F D QR+A +A I+GLN L Sbjct: 113 ETAFSNIQLIAAYFTKIKAIAEAELIGSVSDVVISIPAWFTDIQRRALLEAANIAGLNPL 172 Query: 573 RIINEPTAAAIAYGLDK 623 R++N+ AAA+ YG+ K Sbjct: 173 RLMNDNAAAALTYGITK 189 >SPAC57A7.12 |||heat shock protein Pdr13 |Schizosaccharomyces pombe|chr 1|||Manual Length = 566 Score = 68.5 bits (160), Expect = 1e-12 Identities = 40/137 (29%), Positives = 74/137 (54%), Gaps = 6/137 (4%) Frame = +3 Query: 216 GDAAKNQVAMNPNNTIFDAKRLIGRKFEDATVQADMKHWPFEVVSDGGKPKIKVAYKGED 395 G A+ Q+ N +N++ + + L+G+ ++ T+ H+ V+ G+ K+ + ++ Sbjct: 72 GVQARGQLVRNADNSVTNFRDLLGKSHDELTLHHC--HYSANPVNVEGQIGFKITVQEDE 129 Query: 396 ------KTFFPEEVSSMVLTKMKETAEAYLGKTVQNAVITVPAYFNDSQRQATKDAGTIS 557 K E S L ++ E+AE +LG V V++VP YF D+QR+A + A + Sbjct: 130 ESDPKEKILTAHEASVRHLRRLTESAEDFLGTKVNGCVMSVPVYFTDAQRKALESAANEA 189 Query: 558 GLNVLRIINEPTAAAIA 608 GL VL++I++P A +A Sbjct: 190 GLPVLQLIHDPAAVILA 206 >SPAC1F5.06 |||heat shock protein Lhs1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 848 Score = 50.8 bits (116), Expect = 2e-07 Identities = 46/187 (24%), Positives = 83/187 (44%), Gaps = 2/187 (1%) Frame = +3 Query: 216 GDAAKNQVAMNPNNTIFDAKRLIGRK-FEDATVQADMKHWPFEVVSDGGKPKIKVAYK-G 389 G A N P ++I + K L+ E VQ +P + + + +++ Sbjct: 71 GVDASNLATRFPAHSIRNVKELLDTAGLESVLVQKYQSSYPAIQLVENEETTSGISFVIS 130 Query: 390 EDKTFFPEEVSSMVLTKMKETAEAYLGKTVQNAVITVPAYFNDSQRQATKDAGTISGLNV 569 +++ + EE+ +M + AE + + + V+TVP +FN+ QR +A I +V Sbjct: 131 DEENYSLEEIIAMTMEHYISLAEEMAHEKITDLVLTVPPHFNELQRSILLEAARILNKHV 190 Query: 570 LRIINEPTAAAIAYGLDKKGYWRTKCTYL*PRRRYLSTCPSLPSKMVSFEVKSTXGDTHF 749 L +I++ A AI Y L + + T TY Y S S + ++SF+ T + Sbjct: 191 LALIDDNVAVAIEYSLSRS--FSTDPTY---NIIYDSGSGSTSATVISFD---TVEGSSL 242 Query: 750 GKXXTLT 770 GK +T Sbjct: 243 GKKQNIT 249 >SPAC11H11.01 |sst6|cps23|ESCRT I complex subunit Vps23|Schizosaccharomyces pombe|chr 1|||Manual Length = 487 Score = 27.1 bits (57), Expect = 3.4 Identities = 18/63 (28%), Positives = 27/63 (42%), Gaps = 3/63 (4%) Frame = -3 Query: 762 SSPSQSGCRRRWISPRKIPSSMVRMDTSKGTAAEVKDKYISFSSTLFCQDR---KQSQQQ 592 SS S+ R+ P K+P +++ + E SF STLF + + QQ Sbjct: 175 SSSSEQHFRKVPALPSKLPPKPLKITANSSLGQETNSNSSSFQSTLFSLNTAPFSATSQQ 234 Query: 591 SVH 583 VH Sbjct: 235 LVH 237 >SPAC19G12.10c |cpy1|pcy1|vacuolar carboxypeptidase Y|Schizosaccharomyces pombe|chr 1|||Manual Length = 1002 Score = 26.6 bits (56), Expect = 4.4 Identities = 14/40 (35%), Positives = 22/40 (55%) Frame = -1 Query: 671 PPPRSKISTFRSPVPFFVKTVSNRSSSRFIDDSENVQARD 552 P + K+ +F P F+ TVS+R + F DD E+ R+ Sbjct: 93 PGFKDKLDSFLQPARDFLHTVSDRLDNIF-DDDEDEHVRE 131 >SPBC17G9.03c |||lysine-tRNA ligase|Schizosaccharomyces pombe|chr 2|||Manual Length = 591 Score = 25.8 bits (54), Expect = 7.8 Identities = 18/64 (28%), Positives = 28/64 (43%), Gaps = 1/64 (1%) Frame = +3 Query: 447 ETAEAYLG-KTVQNAVITVPAYFNDSQRQATKDAGTISGLNVLRIINEPTAAAIAYGLDK 623 E EA++ K + NA + F+ R + G + +II+E A+ YGL Sbjct: 488 ERFEAFVATKEICNAYTELNDIFDQRARFEEQARQKAQGDDEAQIIDENFCTALEYGLPP 547 Query: 624 KGYW 635 G W Sbjct: 548 TGGW 551 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 3,584,469 Number of Sequences: 5004 Number of extensions: 75112 Number of successful extensions: 228 Number of sequences better than 10.0: 11 Number of HSP's better than 10.0 without gapping: 187 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 208 length of database: 2,362,478 effective HSP length: 72 effective length of database: 2,002,190 effective search space used: 420459900 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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