Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= brP-0116
(848 letters)
Database: nematostella
59,808 sequences; 16,821,457 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SB_45540| Best HMM Match : HSP70 (HMM E-Value=0) 286 1e-77
SB_8490| Best HMM Match : HSP70 (HMM E-Value=0) 279 2e-75
SB_45647| Best HMM Match : HSP70 (HMM E-Value=0) 230 1e-60
SB_56180| Best HMM Match : No HMM Matches (HMM E-Value=.) 193 1e-49
SB_51590| Best HMM Match : HSP70 (HMM E-Value=4.4e-36) 128 4e-30
SB_15336| Best HMM Match : No HMM Matches (HMM E-Value=.) 115 6e-26
SB_57217| Best HMM Match : Helicase_C (HMM E-Value=0.049) 101 1e-21
SB_31398| Best HMM Match : No HMM Matches (HMM E-Value=.) 70 2e-12
SB_19079| Best HMM Match : LCM (HMM E-Value=6.3e-37) 29 4.8
SB_58645| Best HMM Match : Kelch_1 (HMM E-Value=1.9) 29 4.8
SB_24848| Best HMM Match : No HMM Matches (HMM E-Value=.) 29 6.3
SB_42677| Best HMM Match : TUDOR (HMM E-Value=0) 28 8.3
>SB_45540| Best HMM Match : HSP70 (HMM E-Value=0)
Length = 1097
Score = 286 bits (702), Expect = 1e-77
Identities = 154/262 (58%), Positives = 181/262 (69%), Gaps = 1/262 (0%)
Frame = +3
Query: 63 KHPQ*AIDLGTTYSCVGVFQHG*GGDHRQRPGXTGTTSVLCCVHXTPSVSSGDAAKNQVA 242
K P IDLGTTYSCVGVFQHG G T S + + GDAAKNQVA
Sbjct: 3 KTPAVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFSDTERLI--GDAAKNQVA 60
Query: 243 MNPNNTIFDAKRLIGRKFEDATVQADMKHWPFEVVSDGGKPKIKVAYKGEDKTFFPEEVS 422
MNP NT+FDAKRLIGRKF+D V DM HWPF V+ +G +PKI+V +KGE K+FFPEE+S
Sbjct: 61 MNPENTVFDAKRLIGRKFDDPVVARDMTHWPFHVIREGERPKIQVEFKGEKKSFFPEEIS 120
Query: 423 SMVLTKMKETAEAYLGKTVQNAVITVPAYFNDSQRQATKDAGTISGLNVLRIINEPTAAA 602
SMVLTKMKETAEAYLG V +AV+TVPAYF+DSQRQATKDAGTI+GLNVLRIINEPTAAA
Sbjct: 121 SMVLTKMKETAEAYLGAKVTDAVVTVPAYFHDSQRQATKDAGTIAGLNVLRIINEPTAAA 180
Query: 603 IAYGLDKK-GYWRTKCTYL*PRRRYLSTCPSLPSKMVSFEVKSTXGDTHFGKXXTLTIRM 779
IAYGLDKK G R Y + + ++ + FEVKST GDTH G +M
Sbjct: 181 IAYGLDKKVGVERNVLIYDLGGGTFDVSVLTIEDGI--FEVKSTAGDTHLG-GEDFDNKM 237
Query: 780 VNHFVQEFRRNSKXASLPTKNS 845
V+HFV++F++ K P K +
Sbjct: 238 VDHFVKQFKQKYKKDITPNKRA 259
>SB_8490| Best HMM Match : HSP70 (HMM E-Value=0)
Length = 640
Score = 279 bits (684), Expect = 2e-75
Identities = 152/252 (60%), Positives = 171/252 (67%)
Frame = +3
Query: 63 KHPQ*AIDLGTTYSCVGVFQHG*GGDHRQRPGXTGTTSVLCCVHXTPSVSSGDAAKNQVA 242
K P IDLGTTYSCVGVFQ+G G T S + V GDAAKNQVA
Sbjct: 3 KAPAIGIDLGTTYSCVGVFQNGKVEIIANDQGNRTTPSYVGFTDTERLV--GDAAKNQVA 60
Query: 243 MNPNNTIFDAKRLIGRKFEDATVQADMKHWPFEVVSDGGKPKIKVAYKGEDKTFFPEEVS 422
+NP NTIFDAKRLIGR+++DATVQ+DMK WPF+++SD KPKI+V YKGE KTF EE+S
Sbjct: 61 LNPENTIFDAKRLIGRRYDDATVQSDMKLWPFKIISDNNKPKIQVEYKGERKTFAAEEIS 120
Query: 423 SMVLTKMKETAEAYLGKTVQNAVITVPAYFNDSQRQATKDAGTISGLNVLRIINEPTAAA 602
SMVL KMKETAEAYLG+ V +AVITVPAYFNDSQRQATKDAGTI+GLNVLR+INEPTAAA
Sbjct: 121 SMVLGKMKETAEAYLGQKVTSAVITVPAYFNDSQRQATKDAGTIAGLNVLRVINEPTAAA 180
Query: 603 IAYGLDKKGYWRTKCTYL*PRRRYLSTCPSLPSKMVSFEVKSTXGDTHFGKXXTLTIRMV 782
+AYGLDK FEVKST GDTH G R+V
Sbjct: 181 LAYGLDKSLSGEKNVLIFDLGGGTFDVSILTIDDGSLFEVKSTAGDTHLG-GEDFDNRLV 239
Query: 783 NHFVQEFRRNSK 818
NHFV EF+R K
Sbjct: 240 NHFVAEFKRKYK 251
Score = 35.5 bits (78), Expect = 0.055
Identities = 15/19 (78%), Positives = 17/19 (89%)
Frame = +1
Query: 622 KKGTGERNVLIFDLGGGTF 678
K +GE+NVLIFDLGGGTF
Sbjct: 187 KSLSGEKNVLIFDLGGGTF 205
>SB_45647| Best HMM Match : HSP70 (HMM E-Value=0)
Length = 1327
Score = 230 bits (562), Expect = 1e-60
Identities = 124/243 (51%), Positives = 162/243 (66%)
Frame = +3
Query: 81 IDLGTTYSCVGVFQHG*GGDHRQRPGXTGTTSVLCCVHXTPSVSSGDAAKNQVAMNPNNT 260
IDLGTTYSCVGVF++G G T S + + GD AKNQ+ NP NT
Sbjct: 706 IDLGTTYSCVGVFKNGRVEIIANDQGNRITPSYVAFTEEGERLI-GDGAKNQLTTNPENT 764
Query: 261 IFDAKRLIGRKFEDATVQADMKHWPFEVVSDGGKPKIKVAYKGEDKTFFPEEVSSMVLTK 440
+FDAKRLIGR ++D +VQ D+K +PF+VV KP ++V K E KTF EE+S+MVLTK
Sbjct: 765 VFDAKRLIGRSWDDTSVQNDLKFFPFKVVDKNKKPHVQVKVKDEIKTFAAEEISAMVLTK 824
Query: 441 MKETAEAYLGKTVQNAVITVPAYFNDSQRQATKDAGTISGLNVLRIINEPTAAAIAYGLD 620
MKETAEAYLGK V +AV+TVPAYFND+QRQATKDAG I+GL V+RIINEPTAAAIAYG+D
Sbjct: 825 MKETAEAYLGKKVTHAVVTVPAYFNDAQRQATKDAGAIAGLTVMRIINEPTAAAIAYGMD 884
Query: 621 KKGYWRTKCTYL*PRRRYLSTCPSLPSKMVSFEVKSTXGDTHFGKXXTLTIRMVNHFVQE 800
KK + + + + ++ + + FEV +T GDTH G ++ HF++
Sbjct: 885 KKEGEKNILVFDLGGGTFDVSLLTIDNGV--FEVVATNGDTHLG-GEDFDQNVMEHFIKL 941
Query: 801 FRR 809
+++
Sbjct: 942 YKK 944
Score = 34.7 bits (76), Expect = 0.096
Identities = 13/18 (72%), Positives = 16/18 (88%)
Frame = +1
Query: 625 KGTGERNVLIFDLGGGTF 678
K GE+N+L+FDLGGGTF
Sbjct: 885 KKEGEKNILVFDLGGGTF 902
>SB_56180| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 746
Score = 193 bits (471), Expect = 1e-49
Identities = 111/187 (59%), Positives = 121/187 (64%), Gaps = 25/187 (13%)
Frame = +3
Query: 63 KHPQ*AIDLGTTYSCVGVFQHG*GGDHRQRPGXTGTTSVLCCVHXTPSVSSGDAAKNQVA 242
K P IDLGTTYSCVGVFQHG G T S + V GDAAKNQVA
Sbjct: 3 KAPAIGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDEERLV--GDAAKNQVA 60
Query: 243 MNPNNTIFDAKRLIGRKFEDATVQADMKHWPFEVVSDGGKPKIKVAYKGEDKTFFPEEV- 419
MNP NTIFDAKRLIGR+F+D V+ DMKHW FEVV++ G+PK+KV YKGE KTFF EEV
Sbjct: 61 MNPTNTIFDAKRLIGRRFDDPGVKDDMKHWSFEVVNEAGRPKVKVEYKGETKTFFAEEVV 120
Query: 420 ------------------------SSMVLTKMKETAEAYLGKTVQNAVITVPAYFNDSQR 527
SSMVL KMKETAEAYLG V +AV+TVPAYFNDSQR
Sbjct: 121 NEAGRPKVKVEYKGETKTFFAEEISSMVLNKMKETAEAYLGCKVTDAVVTVPAYFNDSQR 180
Query: 528 QATKDAG 548
QATKDAG
Sbjct: 181 QATKDAG 187
Score = 146 bits (354), Expect = 2e-35
Identities = 84/145 (57%), Positives = 99/145 (68%), Gaps = 1/145 (0%)
Frame = +3
Query: 414 EVSSMVLTKMKETAEAYLGKTVQNAVITVPAYFNDSQRQATKDAGTISGLNVLRIINEPT 593
++SSMVL KMKETAEAYLG V +AV+TVPAYFNDSQRQATKDAG ISGLNVLRIINEPT
Sbjct: 211 QISSMVLNKMKETAEAYLGCKVTDAVVTVPAYFNDSQRQATKDAGVISGLNVLRIINEPT 270
Query: 594 AAAIAYGLDKK-GYWRTKCTYL*PRRRYLSTCPSLPSKMVSFEVKSTXGDTHFGKXXTLT 770
AAAIAYGLDKK G R + + + S+ + FEVKST GDTH G
Sbjct: 271 AAAIAYGLDKKVGQERNVLIFDLGGGTFDVSVLSIEDGI--FEVKSTHGDTHLG-GEDFD 327
Query: 771 IRMVNHFVQEFRRNSKXASLPTKNS 845
MV+ FV++F++ K P K +
Sbjct: 328 NNMVDFFVKQFKQKYKKDITPNKRA 352
>SB_51590| Best HMM Match : HSP70 (HMM E-Value=4.4e-36)
Length = 437
Score = 128 bits (310), Expect = 4e-30
Identities = 76/183 (41%), Positives = 98/183 (53%), Gaps = 1/183 (0%)
Frame = +3
Query: 81 IDLGTTYSCVGVFQHG*GGDHRQRPGXTGTTSVLCCVHXTPSVSSGDAAKNQVAMNPNNT 260
IDLGTTYSCVG +Q G V G+ A +Q +NP NT
Sbjct: 36 IDLGTTYSCVGAYQAISGRIDIFSDENDDKVVPSLVAFTDKGVLVGEKALSQAEINPKNT 95
Query: 261 IFDAKRLIGRKFEDATVQADMKHWPFEVVSDG-GKPKIKVAYKGEDKTFFPEEVSSMVLT 437
I+DAKR IG+ F + K + F V +D G V KG PEE+ V+
Sbjct: 96 IYDAKRFIGKTFTKQELLEIGKLYQFRVTTDKRGNAGFLVGKKGNFTVITPEEIGGYVIK 155
Query: 438 KMKETAEAYLGKTVQNAVITVPAYFNDSQRQATKDAGTISGLNVLRIINEPTAAAIAYGL 617
K++ AE L TV AV++VPA F+ QR AT A +++G+ VLR+INEPTAAA+AYGL
Sbjct: 156 KLRTMAERNLTTTVTKAVMSVPAEFDIKQRNATVKAASLAGITVLRVINEPTAAALAYGL 215
Query: 618 DKK 626
KK
Sbjct: 216 HKK 218
>SB_15336| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 1068
Score = 115 bits (276), Expect = 6e-26
Identities = 71/195 (36%), Positives = 104/195 (53%), Gaps = 4/195 (2%)
Frame = +3
Query: 246 NPNNTIFDAKRLIGRKFEDATVQADMKHWPFEVVS-DGGKPKIKVAYKGEDKTFFPEEVS 422
N NTI KR IGRKF D VQ ++ H P++VV I+V Y G+ + F PE+V
Sbjct: 118 NLKNTISQFKRFIGRKFSDPAVQKEIPHLPYKVVELPNDSIGIQVQYLGKQEVFSPEQVM 177
Query: 423 SMVLTKMKETAEAYLGKTVQNAVITVPAYFNDSQRQATKDAGTISGLNVLRIINEPTAAA 602
+M+ T++K TAE L V + VI+VP+Y+ D QR+ DA +GLN LR++N+ TA +
Sbjct: 178 AMLFTRLKTTAEIALKTKVTDCVISVPSYYTDRQRRCMLDASATAGLNCLRLMNDTTAVS 237
Query: 603 IAYGLDKKGYWRTK---CTYL*PRRRYLSTCPSLPSKMVSFEVKSTXGDTHFGKXXTLTI 773
+AYG+ K+ K ++ L C + K +V ST + + G
Sbjct: 238 LAYGIYKQDLPTDKPRNVVFVDIGHSSLQVCITAFLK-GQLKVLSTAVEPNLG-GRDFDY 295
Query: 774 RMVNHFVQEFRRNSK 818
+V HF QEF+ K
Sbjct: 296 VLVEHFAQEFKTKYK 310
>SB_57217| Best HMM Match : Helicase_C (HMM E-Value=0.049)
Length = 179
Score = 101 bits (241), Expect = 1e-21
Identities = 59/109 (54%), Positives = 74/109 (67%), Gaps = 24/109 (22%)
Frame = +3
Query: 369 IKVAYKGEDKTFFPEEVSSMVLTKMKETAEAYLGKTVQNAVITVPAYFNDSQRQATKDAG 548
I V GE ++F PE++S++VL ++K AEA+LG+ V+ AVITVPA+FND+QRQATKDAG
Sbjct: 2 IAVEVCGEKRSFAPEQISAVVLEQLKADAEAFLGQAVKRAVITVPAHFNDAQRQATKDAG 61
Query: 549 TISGLN------------------------VLRIINEPTAAAIAYGLDK 623
TI+GL+ VLRIINEPTAAAIAYGLDK
Sbjct: 62 TIAGLDVLSRTASERPPSRFRRRRPHGRSRVLRIINEPTAAAIAYGLDK 110
>SB_31398| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 1019
Score = 70.1 bits (164), Expect = 2e-12
Identities = 34/85 (40%), Positives = 52/85 (61%)
Frame = +3
Query: 363 PKIKVAYKGEDKTFFPEEVSSMVLTKMKETAEAYLGKTVQNAVITVPAYFNDSQRQATKD 542
P +K Y+ D TF PEE+ M+L + E + +++ V+TVP +FN ++R+A
Sbjct: 68 PHVK-NYQQSDVTFTPEELMGMILNHSRFIGEQFADHPIKDVVLTVPPFFNQAERRALLR 126
Query: 543 AGTISGLNVLRIINEPTAAAIAYGL 617
A + GLNVL+I+N TA A+ YGL
Sbjct: 127 AAELVGLNVLQIMNSNTAVALNYGL 151
>SB_19079| Best HMM Match : LCM (HMM E-Value=6.3e-37)
Length = 420
Score = 29.1 bits (62), Expect = 4.8
Identities = 9/16 (56%), Positives = 11/16 (68%)
Frame = -3
Query: 267 RILCCWGSSPPGSWRH 220
R++CC G SP WRH
Sbjct: 395 RVVCCQGDSPSPRWRH 410
>SB_58645| Best HMM Match : Kelch_1 (HMM E-Value=1.9)
Length = 340
Score = 29.1 bits (62), Expect = 4.8
Identities = 9/16 (56%), Positives = 11/16 (68%)
Frame = -3
Query: 267 RILCCWGSSPPGSWRH 220
R++CC G SP WRH
Sbjct: 170 RVVCCQGDSPSPRWRH 185
>SB_24848| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 1506
Score = 28.7 bits (61), Expect = 6.3
Identities = 20/68 (29%), Positives = 32/68 (47%)
Frame = -2
Query: 457 SAVSFIFVSTMELTSSGKKVLSSPLYATLILGLPPSLTTSKGQCFMSACTVASSNLRPMR 278
S F+F ST + +S +KV+ SPL + + P+ G S C V NL
Sbjct: 1108 SPAKFVFTSTGIVRASTRKVVPSPLRRSKVPPTVPAQRLFNGDRVESICNV--QNLSRCP 1165
Query: 277 RLASNIVL 254
++++ VL
Sbjct: 1166 KISNATVL 1173
>SB_42677| Best HMM Match : TUDOR (HMM E-Value=0)
Length = 1150
Score = 28.3 bits (60), Expect = 8.3
Identities = 13/40 (32%), Positives = 24/40 (60%)
Frame = -3
Query: 768 SKSSPSQSGCRRRWISPRKIPSSMVRMDTSKGTAAEVKDK 649
S SS +S C+R S + + +S V+ TSKG++ + + +
Sbjct: 229 STSSREESSCKRTSPSSKSMKTSAVKEPTSKGSSPQSQQR 268
Database: nematostella
Posted date: Oct 22, 2007 1:22 PM
Number of letters in database: 16,821,457
Number of sequences in database: 59,808
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 27,968,070
Number of Sequences: 59808
Number of extensions: 620805
Number of successful extensions: 1645
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 1507
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1622
length of database: 16,821,457
effective HSP length: 81
effective length of database: 11,977,009
effective search space used: 2407378809
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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