BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= brP-0111 (454 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At4g08180.3 68417.m01353 oxysterol-binding family protein simila... 75 3e-14 At4g08180.2 68417.m01352 oxysterol-binding family protein simila... 75 3e-14 At4g08180.1 68417.m01351 oxysterol-binding family protein simila... 75 3e-14 At4g22540.2 68417.m03252 oxysterol-binding family protein simila... 72 2e-13 At4g22540.1 68417.m03253 oxysterol-binding family protein simila... 72 2e-13 At2g31020.1 68415.m03782 oxysterol-binding family protein simila... 72 2e-13 At1g13170.1 68414.m01527 oxysterol-binding family protein simila... 72 2e-13 At4g12460.1 68417.m01971 oxysterol-binding family protein simila... 70 9e-13 At2g31030.1 68415.m03783 oxysterol-binding family protein simila... 63 7e-11 At3g09300.1 68416.m01104 oxysterol-binding family protein simila... 51 4e-07 At5g02100.1 68418.m00131 oxysterol-binding family protein simila... 49 2e-06 At5g59420.1 68418.m07446 oxysterol-binding family protein simila... 47 5e-06 At5g51450.1 68418.m06378 zinc finger (C3HC4-type RING finger) fa... 28 2.6 At3g58500.1 68416.m06520 serine/threonine protein phosphatase PP... 27 7.8 At1g20650.1 68414.m02587 protein kinase family protein contains ... 27 7.8 >At4g08180.3 68417.m01353 oxysterol-binding family protein similar to SP|Q969R2 Oxysterol-binding protein 2 {Homo sapiens}; contains Pfam profiles PF00169: PH domain, PF01237: Oxysterol-binding protein Length = 813 Score = 74.5 bits (175), Expect = 3e-14 Identities = 55/154 (35%), Positives = 78/154 (50%), Gaps = 5/154 (3%) Frame = +3 Query: 3 KLWVDNHGDXDIVGDAGPATGYVAHLKYLPYGYFSKDTQRKVTGVVKDPNGVPRYVLQGH 182 KL+ D++G I G A Y LK+ ++ + V G+V++ +G + G Sbjct: 607 KLYCDHYGTMRIEGSAE----YSCKLKFKEQSIIDRNPHQ-VHGIVQNKSGKTVATMFGK 661 Query: 183 WDSRVE-VAPVTSTSADNSVCKTGKFVTAWQRVAAPPDSDKWYNFTVLAAQLNEPEPGVA 359 WD + V S S +G + W+R + PP + YN T A LNE PG+ Sbjct: 662 WDESIHFVTGDCSGKGKLSEDMSGAQLL-WKR-SKPPGNATKYNLTRFAITLNELTPGLK 719 Query: 360 ----PTDSRLRPDQRLMXEGCWDEANTEKLRLEE 449 PTDSRLRPDQR + G ++ ANTEKLRLE+ Sbjct: 720 ERLPPTDSRLRPDQRYLENGEFEMANTEKLRLEQ 753 >At4g08180.2 68417.m01352 oxysterol-binding family protein similar to SP|Q969R2 Oxysterol-binding protein 2 {Homo sapiens}; contains Pfam profiles PF00169: PH domain, PF01237: Oxysterol-binding protein Length = 813 Score = 74.5 bits (175), Expect = 3e-14 Identities = 55/154 (35%), Positives = 78/154 (50%), Gaps = 5/154 (3%) Frame = +3 Query: 3 KLWVDNHGDXDIVGDAGPATGYVAHLKYLPYGYFSKDTQRKVTGVVKDPNGVPRYVLQGH 182 KL+ D++G I G A Y LK+ ++ + V G+V++ +G + G Sbjct: 607 KLYCDHYGTMRIEGSAE----YSCKLKFKEQSIIDRNPHQ-VHGIVQNKSGKTVATMFGK 661 Query: 183 WDSRVE-VAPVTSTSADNSVCKTGKFVTAWQRVAAPPDSDKWYNFTVLAAQLNEPEPGVA 359 WD + V S S +G + W+R + PP + YN T A LNE PG+ Sbjct: 662 WDESIHFVTGDCSGKGKLSEDMSGAQLL-WKR-SKPPGNATKYNLTRFAITLNELTPGLK 719 Query: 360 ----PTDSRLRPDQRLMXEGCWDEANTEKLRLEE 449 PTDSRLRPDQR + G ++ ANTEKLRLE+ Sbjct: 720 ERLPPTDSRLRPDQRYLENGEFEMANTEKLRLEQ 753 >At4g08180.1 68417.m01351 oxysterol-binding family protein similar to SP|Q969R2 Oxysterol-binding protein 2 {Homo sapiens}; contains Pfam profiles PF00169: PH domain, PF01237: Oxysterol-binding protein Length = 814 Score = 74.5 bits (175), Expect = 3e-14 Identities = 55/154 (35%), Positives = 78/154 (50%), Gaps = 5/154 (3%) Frame = +3 Query: 3 KLWVDNHGDXDIVGDAGPATGYVAHLKYLPYGYFSKDTQRKVTGVVKDPNGVPRYVLQGH 182 KL+ D++G I G A Y LK+ ++ + V G+V++ +G + G Sbjct: 608 KLYCDHYGTMRIEGSAE----YSCKLKFKEQSIIDRNPHQ-VHGIVQNKSGKTVATMFGK 662 Query: 183 WDSRVE-VAPVTSTSADNSVCKTGKFVTAWQRVAAPPDSDKWYNFTVLAAQLNEPEPGVA 359 WD + V S S +G + W+R + PP + YN T A LNE PG+ Sbjct: 663 WDESIHFVTGDCSGKGKLSEDMSGAQLL-WKR-SKPPGNATKYNLTRFAITLNELTPGLK 720 Query: 360 ----PTDSRLRPDQRLMXEGCWDEANTEKLRLEE 449 PTDSRLRPDQR + G ++ ANTEKLRLE+ Sbjct: 721 ERLPPTDSRLRPDQRYLENGEFEMANTEKLRLEQ 754 >At4g22540.2 68417.m03252 oxysterol-binding family protein similar to SP|P16258 Oxysterol-binding protein 1 {Oryctolagus cuniculus}; contains Pfam profiles PF00169: PH domain, PF01237: Oxysterol-binding protein Length = 510 Score = 72.1 bits (169), Expect = 2e-13 Identities = 50/152 (32%), Positives = 69/152 (45%), Gaps = 4/152 (2%) Frame = +3 Query: 3 KLWVDNHGDXDIVGDAGPATGYVAHLKYLPYGYFSKDTQRKVTGVVKDPNGVPRYVLQGH 182 KL+ D+HG I G+ Y LK+ ++ + V G V+D G + G Sbjct: 304 KLYCDHHGVMQIRGNRQ----YSCTLKFKEQSILERNPHQ-VNGFVEDVAGQKAATIFGK 358 Query: 183 WDSRVEVAPVTSTSADNSVCKTGKFVTAWQRVAAPPDSDKWYNFTVLAAQLNEPEPGVA- 359 WD + + W+R PP+ + YN T A LNE PG+ Sbjct: 359 WDDSLYYVAGDGINKSKVSDPASNASLLWKRTKPPPNVTR-YNLTSFAITLNELTPGLQE 417 Query: 360 ---PTDSRLRPDQRLMXEGCWDEANTEKLRLE 446 PTDSRLRPDQR + G +++AN EK RLE Sbjct: 418 ILPPTDSRLRPDQRHLENGEYEKANLEKQRLE 449 >At4g22540.1 68417.m03253 oxysterol-binding family protein similar to SP|P16258 Oxysterol-binding protein 1 {Oryctolagus cuniculus}; contains Pfam profiles PF00169: PH domain, PF01237: Oxysterol-binding protein Length = 721 Score = 72.1 bits (169), Expect = 2e-13 Identities = 50/152 (32%), Positives = 69/152 (45%), Gaps = 4/152 (2%) Frame = +3 Query: 3 KLWVDNHGDXDIVGDAGPATGYVAHLKYLPYGYFSKDTQRKVTGVVKDPNGVPRYVLQGH 182 KL+ D+HG I G+ Y LK+ ++ + V G V+D G + G Sbjct: 515 KLYCDHHGVMQIRGNRQ----YSCTLKFKEQSILERNPHQ-VNGFVEDVAGQKAATIFGK 569 Query: 183 WDSRVEVAPVTSTSADNSVCKTGKFVTAWQRVAAPPDSDKWYNFTVLAAQLNEPEPGVA- 359 WD + + W+R PP+ + YN T A LNE PG+ Sbjct: 570 WDDSLYYVAGDGINKSKVSDPASNASLLWKRTKPPPNVTR-YNLTSFAITLNELTPGLQE 628 Query: 360 ---PTDSRLRPDQRLMXEGCWDEANTEKLRLE 446 PTDSRLRPDQR + G +++AN EK RLE Sbjct: 629 ILPPTDSRLRPDQRHLENGEYEKANLEKQRLE 660 >At2g31020.1 68415.m03782 oxysterol-binding family protein similar to SP|Q969R2 Oxysterol-binding protein 2 {Homo sapiens}; contains Pfam profiles PF00169: PH domain, PF01237: Oxysterol-binding protein Length = 760 Score = 72.1 bits (169), Expect = 2e-13 Identities = 52/153 (33%), Positives = 72/153 (47%), Gaps = 4/153 (2%) Frame = +3 Query: 3 KLWVDNHGDXDIVGDAGPATGYVAHLKYLPYGYFSKDTQRKVTGVVKDPNGVPRYVLQGH 182 KL+ D++G I G+ Y LK+ ++ + V G+V+D NG L G Sbjct: 564 KLYCDHYGTMKIEGNGE----YSCKLKFKEQSMIDRNPHQ-VQGIVEDKNGKTVARLFGK 618 Query: 183 WDSRVEVAPVTSTSADNSVCKTGKFVTAWQRVAAPPDSDKWYNFTVLAAQLNEPEPGVA- 359 WD + V + S W+R P + K YN T LNE PG+ Sbjct: 619 WDESIHYVMVDQGKVNESHL-------LWKRNKQPENPTK-YNLTRFGITLNELTPGLKE 670 Query: 360 ---PTDSRLRPDQRLMXEGCWDEANTEKLRLEE 449 PTDSRLRPDQR + +G ++ N EKLRLE+ Sbjct: 671 KLPPTDSRLRPDQRYLEKGEYEMGNAEKLRLEQ 703 >At1g13170.1 68414.m01527 oxysterol-binding family protein similar to SP|P16258 Oxysterol-binding protein 1 {Oryctolagus cuniculus}; contains Pfam profiles PF00169: PH domain, PF01237: Oxysterol-binding protein Length = 816 Score = 71.7 bits (168), Expect = 2e-13 Identities = 52/154 (33%), Positives = 74/154 (48%), Gaps = 5/154 (3%) Frame = +3 Query: 3 KLWVDNHGDXDIVGDAGPATGYVAHLKYLPYGYFSKDTQRKVTGVVKDPN-GVPRYVLQG 179 KL+ D++G I G + Y LK+ ++ R+V G V+D G +L G Sbjct: 604 KLYCDHYGTMRIKG----GSNYSCRLKFKEQSVIDRNP-RQVHGFVQDNRTGEKVAILIG 658 Query: 180 HWDSRVEVAPVTSTSADNSVCKTGKFVTAWQRVAAPPDSDKWYNFTVLAAQLNEPEPGVA 359 WD + T+ + V W+R +P + YN + A LNE PG+ Sbjct: 659 KWDEAMYYVLGDPTTKPKGYDPMTEAVLLWERDKSPTKTR--YNLSPFAISLNEITPGMI 716 Query: 360 ----PTDSRLRPDQRLMXEGCWDEANTEKLRLEE 449 PTDSRLRPDQR + G ++ AN EKLRLE+ Sbjct: 717 DKLPPTDSRLRPDQRHLENGEYESANAEKLRLEQ 750 >At4g12460.1 68417.m01971 oxysterol-binding family protein similar to SP|P22059 Oxysterol-binding protein 1 {Homo sapiens}; contains Pfam profiles PF00169: PH domain, PF01237: Oxysterol-binding protein Length = 694 Score = 69.7 bits (163), Expect = 9e-13 Identities = 56/153 (36%), Positives = 74/153 (48%), Gaps = 5/153 (3%) Frame = +3 Query: 3 KLWVDNHGDXDIVGDAGPATGYVAHLKYLPYGYFSKDTQRKVTGVVKDPNGVPRYVLQGH 182 KL+ D+HG I G+ Y LK+ ++ + V G V+D G + G Sbjct: 495 KLYCDHHGIMKIRGNRQ----YSCMLKFKEQSILDRNPHQ-VNGFVEDVTGKKAATVFGK 549 Query: 183 W-DSRVEVAPVTSTSADNSVCKTGKFVTAWQRVAAPPDSDKWYNFTVLAAQLNEPEPGVA 359 W DS VA A S+ W+ APP+ + YNFT A LNE PG+ Sbjct: 550 WNDSLYYVAGDGINKASASLL--------WKATKAPPNVTR-YNFTSFAMTLNELIPGLE 600 Query: 360 ----PTDSRLRPDQRLMXEGCWDEANTEKLRLE 446 PTDSRLRPDQR + G +++AN EK RLE Sbjct: 601 EKLPPTDSRLRPDQRHLENGEYEKANEEKQRLE 633 >At2g31030.1 68415.m03783 oxysterol-binding family protein similar to SWH1 [Saccharomyces cerevisiae] GI:402658; contains Pfam profile PF01237: Oxysterol-binding protein Length = 489 Score = 63.3 bits (147), Expect = 7e-11 Identities = 50/153 (32%), Positives = 70/153 (45%), Gaps = 4/153 (2%) Frame = +3 Query: 3 KLWVDNHGDXDIVGDAGPATGYVAHLKYLPYGYFSKDTQRKVTGVVKDPNGVPRYVLQGH 182 KL+ D++G I G+ Y LK+ ++ + V G+V+D NG L G Sbjct: 297 KLYCDHYGTMLIEGNGE----YSCKLKFKKQSMMDRNPHQ-VQGIVEDKNGKTVAKLFGK 351 Query: 183 WDSRVEVAPVTSTSADNSVCKTGKFVTAWQRVAAPPDSDKWYNFTVLAAQLNEPEPGVA- 359 WD + V S W+R P ++ YN T LNE P + Sbjct: 352 WDESMYYVMVNQGKESESHL-------LWKR-NKPLENPTKYNLTRFGITLNELTPDLKE 403 Query: 360 ---PTDSRLRPDQRLMXEGCWDEANTEKLRLEE 449 PTDSRLRPDQR + +G ++ N EKLRLE+ Sbjct: 404 MLPPTDSRLRPDQRYLEKGEFEMGNREKLRLEQ 436 >At3g09300.1 68416.m01104 oxysterol-binding family protein similar to SP|P22059 Oxysterol-binding protein 1 {Homo sapiens}; contains Pfam profile PF01237: Oxysterol-binding protein Length = 458 Score = 50.8 bits (116), Expect = 4e-07 Identities = 39/152 (25%), Positives = 68/152 (44%), Gaps = 3/152 (1%) Frame = +3 Query: 3 KLWVDNHGDXDIVGDAGPATGYVAHLKYLPYGYFSKDTQRKVTGVVKDPNGVPRYVLQGH 182 + WVD+ G+ + TG L + P G+F + +V G V + + P+ ++ G Sbjct: 241 RTWVDSPGEMIMTNQT---TGDKVVLYFQPCGWFGSG-RYEVDGYVYNASEEPKILMTGK 296 Query: 183 WDSRVEVAPVTSTSADNSVCKTGKFVTAWQRVAAPPDSDKWYNFTVLAAQLNEPEPG--- 353 W+ + P + + W+ P D DK Y +T A ++N + Sbjct: 297 WNESMSYQPC---DGEGEPLPGTELKEVWKLADVPKD-DK-YQYTHFAHKINSFDTAPKK 351 Query: 354 VAPTDSRLRPDQRLMXEGCWDEANTEKLRLEE 449 + P+DSRLRPD+ + G ++ EK +EE Sbjct: 352 LLPSDSRLRPDRYALEMGDMSKSGYEKSSMEE 383 >At5g02100.1 68418.m00131 oxysterol-binding family protein similar to SWH1 [Saccharomyces cerevisiae] GI:402658; contains Pfam profile PF01237: Oxysterol-binding protein Length = 453 Score = 48.8 bits (111), Expect = 2e-06 Identities = 40/152 (26%), Positives = 69/152 (45%), Gaps = 3/152 (1%) Frame = +3 Query: 3 KLWVDNHGDXDIVGDAGPATGYVAHLKYLPYGYFSKDTQRKVTGVVKDPNGVPRYVLQGH 182 + WVD+ G+ + TG L + P G+F + +V G V + P+ ++ G Sbjct: 236 RTWVDSPGEMVMTN---LTTGDKVVLYFQPCGWFGSG-RYEVDGYVYNSAEEPKMLMTGK 291 Query: 183 WDSRVEVAPVTSTSADNSVCKTGKFVTAWQRVAAPPDSDKWYNFTVLAAQLNEPEPG--- 353 W+ + P A+ + W +VA P +DK Y +T A ++N + Sbjct: 292 WNESLSYQPC---DAEGEPLPGTELKEVW-KVAEAPKNDK-YQYTHFAHKINSFDTAPKK 346 Query: 354 VAPTDSRLRPDQRLMXEGCWDEANTEKLRLEE 449 + +DSRLRPD+ + G ++ EK LE+ Sbjct: 347 LLSSDSRLRPDRYALEMGDMSKSGFEKSSLED 378 >At5g59420.1 68418.m07446 oxysterol-binding family protein similar to SP|P16258 Oxysterol-binding protein 1 {Oryctolagus cuniculus}; contains Pfam profile PF01237: Oxysterol-binding protein Length = 457 Score = 47.2 bits (107), Expect = 5e-06 Identities = 39/152 (25%), Positives = 69/152 (45%), Gaps = 3/152 (1%) Frame = +3 Query: 3 KLWVDNHGDXDIVGDAGPATGYVAHLKYLPYGYFSKDTQRKVTGVVKDPNGVPRYVLQGH 182 + WVD+ G+ + TG L + P G+F + +V G V P+ ++ G Sbjct: 236 RTWVDSPGEMVMTN---LTTGDKVVLYFQPCGWFGSG-RYEVDGYVYSAAEEPKIMMTGK 291 Query: 183 WDSRVEVAPVTSTSADNSVCKTGKFVTAWQRVAAPPDSDKWYNFTVLAAQLNEPEPGVAP 362 W+ ++ P A+ + W +A P +D + +T A ++N + A Sbjct: 292 WNEKMSYQPC---DAEGEPLPGTELKEVWH-LADVPKNDN-FQYTHFAHKINSFDTAPAK 346 Query: 363 ---TDSRLRPDQRLMXEGCWDEANTEKLRLEE 449 +DSR+RPD+ + +G +A +EK LEE Sbjct: 347 LLASDSRIRPDRYSLEQGDLSKAGSEKHSLEE 378 >At5g51450.1 68418.m06378 zinc finger (C3HC4-type RING finger) family protein contains similarity to autocrine motility factor receptor [Mus musculus] GI:5931953; contains Pfam profiles PF00097: Zinc finger, C3HC4 type (RING finger), PF02845: CUE domain Length = 577 Score = 28.3 bits (60), Expect = 2.6 Identities = 13/44 (29%), Positives = 23/44 (52%) Frame = -1 Query: 418 SSQQPSSMRRWSGRSRESVGATPGSGSLSCAASTVKLYHLSESG 287 S PS ++ WS + + VG++ GS S+ + + HL+ G Sbjct: 439 SEVDPSWLQTWSDQGVDVVGSSAGSRSVGLGQVQLMMRHLASVG 482 >At3g58500.1 68416.m06520 serine/threonine protein phosphatase PP2A-4 catalytic subunit (PP2A4) identical to SP|P48578 Serine/threonine protein phosphatase PP2A-4 catalytic subunit (EC 3.1.3.16) (Protein phosphatase 2A isoform 4) {Arabidopsis thaliana}; contains Pfam profile PF00149: Ser/Thr protein phosphatase Length = 313 Score = 26.6 bits (56), Expect = 7.8 Identities = 16/53 (30%), Positives = 29/53 (54%), Gaps = 1/53 (1%) Frame = +3 Query: 39 VGDAGPATGYVAHLKYLPYGYFSKDTQRKVTGV-VKDPNGVPRYVLQGHWDSR 194 +G P T Y+ Y+ GY+S +T + G+ V+ P + +L+G+ +SR Sbjct: 75 IGGKCPDTNYLFMGDYVDRGYYSVETVTLLVGLKVRYPQRIT--ILRGNHESR 125 >At1g20650.1 68414.m02587 protein kinase family protein contains Pfam domain PF00069: Protein kinase domain Length = 585 Score = 26.6 bits (56), Expect = 7.8 Identities = 15/40 (37%), Positives = 22/40 (55%) Frame = -1 Query: 424 LASSQQPSSMRRWSGRSRESVGATPGSGSLSCAASTVKLY 305 L S+Q+P S W+ R + +VGA G L C A+ +Y Sbjct: 370 LESNQEPLS---WNTRMKIAVGAARGIEYLHCTANPPVIY 406 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 7,357,160 Number of Sequences: 28952 Number of extensions: 141578 Number of successful extensions: 486 Number of sequences better than 10.0: 15 Number of HSP's better than 10.0 without gapping: 474 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 477 length of database: 12,070,560 effective HSP length: 75 effective length of database: 9,899,160 effective search space used: 742437000 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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