Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= brP-0102
(776 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 390 e-109
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 386 e-108
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 196 4e-51
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 155 5e-39
SPBC1718.06 |msp1|mgm1|mitochondrial GTPase Msp1|Schizosaccharom... 31 0.14
SPBC146.13c |myo1||myosin type I|Schizosaccharomyces pombe|chr 2... 27 4.0
SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||... 26 6.9
SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr... 26 6.9
>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 449
Score = 390 bits (960), Expect = e-109
Identities = 173/248 (69%), Positives = 207/248 (83%)
Frame = +2
Query: 32 IGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGKHVPRAVFVDLEPTVV 211
IGNACWELYCLEHGIQP+G M + D F+TFFSETG GK+VPR+++VDLEP V+
Sbjct: 16 IGNACWELYCLEHGIQPNGYMNPETASQNSDGGFSTFFSETGQGKYVPRSIYVDLEPNVI 75
Query: 212 DEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLDRIRKLADQCTGLQGF 391
D+VRTG YR LFHPEQLITGKEDA+NNYARGHYT+GKE+VD V D+IR++AD C+GLQGF
Sbjct: 76 DQVRTGPYRDLFHPEQLITGKEDASNNYARGHYTVGKELVDEVTDKIRRIADNCSGLQGF 135
Query: 392 LIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYPAPQVSTAVVEPYNSILTTHTTL 571
L+FH LL+ERL+++Y KKSKL+F++YPAPQVST+VVEPYNS+LTTH TL
Sbjct: 136 LVFHSFGGGTGSGFGALLLERLAMEYTKKSKLQFSVYPAPQVSTSVVEPYNSVLTTHATL 195
Query: 572 EHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLIGQIVSSITASLKFDGALNVDLTEF 751
+ +DC FMVDNE+ YDICRRNLDIERP+Y NLNRLI Q+VSSITASL+F+G+LNVDL EF
Sbjct: 196 DLADCTFMVDNESCYDICRRNLDIERPSYENLNRLIAQVVSSITASLRFEGSLNVDLAEF 255
Query: 752 XTNLXXYP 775
TNL YP
Sbjct: 256 QTNLVPYP 263
>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 455
Score = 386 bits (950), Expect = e-108
Identities = 176/255 (69%), Positives = 209/255 (81%), Gaps = 5/255 (1%)
Frame = +2
Query: 26 VXIGNACWELYCLEHGIQPDGQMPTD-----KTIGGGDDSFNTFFSETGAGKHVPRAVFV 190
V IGNACWELYCLEHGI PDG PT+ K +D F TFFSETG GK VPR+++V
Sbjct: 14 VQIGNACWELYCLEHGIGPDG-FPTENSEVHKNNSYLNDGFGTFFSETGQGKFVPRSIYV 72
Query: 191 DLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLDRIRKLADQ 370
DLEP V+D+VRTG Y+ LFHPEQ++TGKEDA+NNYARGHYT+GKE++D VL+RIR++AD
Sbjct: 73 DLEPNVIDQVRTGPYKDLFHPEQMVTGKEDASNNYARGHYTVGKEMIDSVLERIRRMADN 132
Query: 371 CTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYPAPQVSTAVVEPYNSI 550
C+GLQGFL+FH LL+ERL+++YGKKS L+F++YPAPQVST+VVEPYNS+
Sbjct: 133 CSGLQGFLVFHSFGGGTGSGLGALLLERLNMEYGKKSNLQFSVYPAPQVSTSVVEPYNSV 192
Query: 551 LTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLIGQIVSSITASLKFDGAL 730
LTTH TL++SDC FMVDNEA YDICRRNLDIERPTY NLNRLI Q+VSSITASL+F G+L
Sbjct: 193 LTTHATLDNSDCTFMVDNEACYDICRRNLDIERPTYENLNRLIAQVVSSITASLRFAGSL 252
Query: 731 NVDLTEFXTNLXXYP 775
NVDL EF TNL YP
Sbjct: 253 NVDLNEFQTNLVPYP 267
>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 448
Score = 196 bits (477), Expect = 4e-51
Identities = 94/248 (37%), Positives = 140/248 (56%)
Frame = +2
Query: 32 IGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGKHVPRAVFVDLEPTVV 211
+G A W EHG+ G T + N +F+E GK+VPRAV VDLEP +
Sbjct: 16 VGAAFWSTIADEHGLDSAGIY--HGTSEAQHERLNVYFNEAAGGKYVPRAVLVDLEPGTM 73
Query: 212 DEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLDRIRKLADQCTGLQGF 391
D V++G + LF P+ +I G+ A N +A+GHYT G E+ D VLD +R+ A+ C LQGF
Sbjct: 74 DAVKSGKFGNLFRPDNIIYGQSGAGNIWAKGHYTEGAELADAVLDVVRREAEACDALQGF 133
Query: 392 LIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYPAPQVSTAVVEPYNSILTTHTTL 571
+ H LL+ ++ +Y + F++ PAP+ S VVEPYN+ L+ H +
Sbjct: 134 QLTHSLGGGTGSGMGTLLLSKIREEYPDRMMATFSVAPAPKSSDTVVEPYNATLSMHQLV 193
Query: 572 EHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLIGQIVSSITASLKFDGALNVDLTEF 751
E+SD F +DNEA+ I L I+ P+Y +LN L+ +++ +T S +F G LN DL +
Sbjct: 194 ENSDETFCIDNEALSSIFANTLKIKSPSYDDLNHLVSAVMAGVTTSFRFPGELNSDLRKL 253
Query: 752 XTNLXXYP 775
N+ +P
Sbjct: 254 AVNMVPFP 261
>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 446
Score = 155 bits (377), Expect = 5e-39
Identities = 84/251 (33%), Positives = 137/251 (54%), Gaps = 3/251 (1%)
Frame = +2
Query: 32 IGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGKHVPRAVFVDLEPTVV 211
IG+ W+ CLEHGI PDG + + T G D + FF ++ +++PRA+ +DLEP VV
Sbjct: 17 IGSQFWQQLCLEHGIGPDGTLESFAT--EGVDRKDVFFYQSDDTRYIPRAILIDLEPRVV 74
Query: 212 DEVRTGTYRQLFHPEQLITGKED--AANNYARGHYTIGKEIVDLVLDRIRKLADQCTGLQ 385
+ + + TY L++PE ++ K A NN+A G Y+ + I + ++D I + AD L+
Sbjct: 75 NNILSDTYGSLYNPENILITKNGGGAGNNWANG-YSHAERIFEDIMDMIDREADGSDSLE 133
Query: 386 GFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYPAPQ-VSTAVVEPYNSILTTH 562
GF + H L+ERL+ Y KK ++++P Q VS VV+PYNS+L
Sbjct: 134 GFSLLHSIAGGTGSGLGSFLLERLNDRYPKKIIQTYSVFPNSQSVSDVVVQPYNSLLALK 193
Query: 563 TTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLIGQIVSSITASLKFDGALNVDL 742
++D ++DN A+ I L + PT+ N+L+ ++S+ T +L++ G +N DL
Sbjct: 194 RLTLNADSVVVLDNAALAHIAADRLHTQNPTFHQQNQLVSTVMSASTTTLRYPGYMNNDL 253
Query: 743 TEFXTNLXXYP 775
+L P
Sbjct: 254 VSIIASLIPSP 264
>SPBC1718.06 |msp1|mgm1|mitochondrial GTPase
Msp1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 903
Score = 31.5 bits (68), Expect = 0.14
Identities = 17/48 (35%), Positives = 28/48 (58%)
Frame = -2
Query: 751 ELGEVHIQSAVEFQRSSDRGDNLSDETIQVGVGWALNVEITAADVIDG 608
E + H ++ + Q+SSD ++ +D T VG+G AL I + D +DG
Sbjct: 171 ERAKEHRSNSNDKQKSSDNDEDPNDTT--VGIGAALAASILSVDSVDG 216
>SPBC146.13c |myo1||myosin type I|Schizosaccharomyces pombe|chr
2|||Manual
Length = 1217
Score = 26.6 bits (56), Expect = 4.0
Identities = 10/34 (29%), Positives = 20/34 (58%)
Frame = -2
Query: 721 VEFQRSSDRGDNLSDETIQVGVGWALNVEITAAD 620
++FQ D G +SD++I +G+ + V + A +
Sbjct: 310 IQFQEGQDGGSVISDKSITEFLGYLIGVPVAAIE 343
>SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr
2|||Manual
Length = 534
Score = 25.8 bits (54), Expect = 6.9
Identities = 15/35 (42%), Positives = 19/35 (54%)
Frame = +3
Query: 66 STASSLMARCPQTRPSGVETILSTLSSARPELAST 170
ST SSL + ++PS T ST SSA P S+
Sbjct: 173 STFSSLSSSTSSSQPSVSSTSSSTFSSAAPTSTSS 207
>SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1275
Score = 25.8 bits (54), Expect = 6.9
Identities = 13/22 (59%), Positives = 13/22 (59%)
Frame = +1
Query: 85 WPDAHRQDHRGWRRFFQHFLQR 150
W A R D R R FQHFLQR
Sbjct: 590 WLAACRSDPRCRRLDFQHFLQR 611
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,042,315
Number of Sequences: 5004
Number of extensions: 62476
Number of successful extensions: 251
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 199
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 247
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 375345278
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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