Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= brP-0100
(724 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC20G4.06c |adf1|cof1|cofilin|Schizosaccharomyces pombe|chr 1|... 98 1e-21
SPCC126.06 |twf1||twinfilin|Schizosaccharomyces pombe|chr 3|||Ma... 38 0.001
SPBP4H10.18c |||sequence orphan|Schizosaccharomyces pombe|chr 2|... 29 0.67
SPCC1450.04 |tef5||translation elongation factor EF-1 beta subun... 27 3.6
SPAC17H9.11 |||cofilin/tropomyosin family protein|Schizosaccharo... 27 3.6
SPAC1D4.11c |lkh1|kic1|dual specificity protein kinase Lkh1|Schi... 26 6.3
SPBP35G2.03c |sgo1||shugoshin Sgo1|Schizosaccharomyces pombe|chr... 25 8.3
SPBC3H7.05c |||sequence orphan|Schizosaccharomyces pombe|chr 2||... 25 8.3
>SPAC20G4.06c |adf1|cof1|cofilin|Schizosaccharomyces pombe|chr
1|||Manual
Length = 137
Score = 98.3 bits (234), Expect = 1e-21
Identities = 54/140 (38%), Positives = 81/140 (57%)
Frame = +3
Query: 57 SGVTVSDACKTTYEEIKKDKKHRYVVFYIRDEKQIDVETVGERNAEYEQFLEDLQKGGTG 236
SGV VS C ++E+K K RYVVF + D K V + +++ FL DL +
Sbjct: 4 SGVKVSPECLEAFQELKLGKSLRYVVFKMNDTKTEIVVEKKSTDKDFDTFLGDLPEK--- 60
Query: 237 ECRYGLFDFEYTHQCQGTSEASKKQKLFLMSWCPDTAKVKKKMLYSSSFDALKKSLVGVQ 416
+CRY ++DFE+ + +G + K+ +SW PD A +K KM+YSSS D L+++ G+
Sbjct: 61 DCRYAIYDFEF-NLGEGV-----RNKIIFISWSPDVAPIKSKMVYSSSKDTLRRAFTGIG 114
Query: 417 KYIQATDLSEASHEAVEXKL 476
IQATD SE ++E V K+
Sbjct: 115 TDIQATDFSEVAYETVLEKV 134
>SPCC126.06 |twf1||twinfilin|Schizosaccharomyces pombe|chr
3|||Manual
Length = 328
Score = 37.9 bits (84), Expect = 0.001
Identities = 28/86 (32%), Positives = 44/86 (51%)
Frame = +3
Query: 123 RYVVFYIRDEKQIDVETVGERNAEYEQFLEDLQKGGTGECRYGLFDFEYTHQCQGTSEAS 302
R + I +E DV+T+ E++ E D +K EC G + + + S
Sbjct: 26 RAAIISISNENSFDVKTMVEKSESIES---DFKK--VRECLLGSEEPAFVL----VYDDS 76
Query: 303 KKQKLFLMSWCPDTAKVKKKMLYSSS 380
KK L L+S+ P+ A V++KMLY+SS
Sbjct: 77 KKNLLQLISYVPENANVRRKMLYASS 102
Score = 27.9 bits (59), Expect = 1.6
Identities = 16/56 (28%), Positives = 31/56 (55%), Gaps = 5/56 (8%)
Frame = +3
Query: 312 KLFLMSWCPDTAKVKKKMLYSSS----FDALKKSL-VGVQKYIQATDLSEASHEAV 464
K+ + CP A VK +M+YSSS D++K L + + I++ D ++ + + +
Sbjct: 244 KILFIYICPMQATVKHRMVYSSSKLGLLDSIKAELGIVIDGKIESNDAADITEKEI 299
>SPBP4H10.18c |||sequence orphan|Schizosaccharomyces pombe|chr
2|||Manual
Length = 314
Score = 29.1 bits (62), Expect = 0.67
Identities = 37/174 (21%), Positives = 68/174 (39%), Gaps = 3/174 (1%)
Frame = +3
Query: 30 LRE*HQKMASGVTVSDACKTTYEEIKKDKKHRYVVFYIRDEKQIDVETVGE-RNAEYEQF 206
LR ++ G D+ ++ +E ++KK + F RD++++ ++ R
Sbjct: 41 LRSEGSELIFGGRSFDSLLSSIKETMEEKKKKSSSFEKRDKRRVQLKEKSPLRTPRNRTQ 100
Query: 207 LEDLQ--KGGTGECRYGLFDFEYTHQCQGTSEASKKQKLFLMSWCPDTAKVKKKMLYSSS 380
+ D + + T +F+ Q T A+K + P T K + ++
Sbjct: 101 IIDARSTRKDTENESLRKNNFKRRTQFAFTGRATKSNAGVMDVQSPSTMSTSKNNVRNAE 160
Query: 381 FDALKKSLVGVQKYIQATDLSEASHEAVEXKLRATDRQ*TAFTHELATKPNPLS 542
A KK + G +KY + S ++ E K R DR + FT LS
Sbjct: 161 RPASKKPVFGSKKYFDVINDSNVENKE-ETKDRNLDRALSKFTQSREKNEQNLS 213
>SPCC1450.04 |tef5||translation elongation factor EF-1 beta subunit
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 214
Score = 26.6 bits (56), Expect = 3.6
Identities = 10/25 (40%), Positives = 18/25 (72%)
Frame = +3
Query: 378 SFDALKKSLVGVQKYIQATDLSEAS 452
S +AL++ L G + Y+Q+TD++ S
Sbjct: 188 SLEALQEELEGFEDYVQSTDIAAMS 212
>SPAC17H9.11 |||cofilin/tropomyosin family
protein|Schizosaccharomyces pombe|chr 1|||Manual
Length = 141
Score = 26.6 bits (56), Expect = 3.6
Identities = 15/60 (25%), Positives = 28/60 (46%)
Frame = +3
Query: 288 TSEASKKQKLFLMSWCPDTAKVKKKMLYSSSFDALKKSLVGVQKYIQATDLSEASHEAVE 467
T++ LF++ W P M+Y+S+ + + V K +A D + + EAV+
Sbjct: 79 TTDGRLSTPLFMIYWRPSATPNDLSMIYASA-KVWFQDVSQVHKVFEARDSEDITSEAVD 137
>SPAC1D4.11c |lkh1|kic1|dual specificity protein kinase
Lkh1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 690
Score = 25.8 bits (54), Expect = 6.3
Identities = 12/44 (27%), Positives = 23/44 (52%), Gaps = 5/44 (11%)
Frame = -1
Query: 331 HDIRKSFCFLLASDVPWHWCVYSKSNRPY-----LHSPVPPFCR 215
HD+ +++ S +H C ++S P+ LH+P+P C+
Sbjct: 107 HDVASHPSYMVQSPTSYHACSNNQSPFPHSHHPPLHNPLPVSCQ 150
>SPBP35G2.03c |sgo1||shugoshin Sgo1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 319
Score = 25.4 bits (53), Expect = 8.3
Identities = 14/61 (22%), Positives = 31/61 (50%)
Frame = +3
Query: 84 KTTYEEIKKDKKHRYVVFYIRDEKQIDVETVGERNAEYEQFLEDLQKGGTGECRYGLFDF 263
K + + ++ + K ++ +D K+ D + + + EY F ++L+ TG+ Y DF
Sbjct: 108 KCSSDFLEANVKSQFTTCENKDSKE-DYQILHNKRLEYVSFNDELKSLETGQPLYCFQDF 166
Query: 264 E 266
+
Sbjct: 167 Q 167
>SPBC3H7.05c |||sequence orphan|Schizosaccharomyces pombe|chr
2|||Manual
Length = 357
Score = 25.4 bits (53), Expect = 8.3
Identities = 13/46 (28%), Positives = 20/46 (43%), Gaps = 2/46 (4%)
Frame = -1
Query: 283 WHWCVYSKSNRPY--LHSPVPPFCRSSRNCSYSALRSPTVSTSICF 152
W++ Y+ S R Y VPP+CR +R+ + CF
Sbjct: 122 WNFVDYALSLRKYGWTDDVVPPYCRKVLTYKSLGIRTAVYVGAFCF 167
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,505,981
Number of Sequences: 5004
Number of extensions: 45267
Number of successful extensions: 145
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 140
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 142
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 339215786
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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