BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= brP-0100 (724 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At5g59890.1 68418.m07510 actin-depolymerizing factor 4 (ADF4) id... 115 4e-26 At3g46010.1 68416.m04978 actin-depolymerizing factor 1 (ADF1) id... 112 3e-25 At5g59890.2 68418.m07511 actin-depolymerizing factor 4 (ADF4) id... 109 2e-24 At4g00680.1 68417.m00093 actin-depolymerizing factor, putative s... 109 2e-24 At1g01750.1 68414.m00094 actin-depolymerizing factor, putative s... 109 2e-24 At5g52360.1 68418.m06497 actin-depolymerizing factor, putative s... 106 1e-23 At5g59880.1 68418.m07508 actin-depolymerizing factor 3 (ADF3) id... 105 3e-23 At3g46000.1 68416.m04977 actin-depolymerizing factor, putative (... 104 7e-23 At2g31200.1 68415.m03810 actin-depolymerizing factor 6 (ADF6) id... 103 9e-23 At4g25590.1 68417.m03687 actin-depolymerizing factor, putative s... 102 3e-22 At2g16700.1 68415.m01916 actin-depolymerizing factor 5 (ADF5) id... 95 6e-20 At4g34970.1 68417.m04957 actin-depolymerizing factor, putative s... 89 4e-18 At3g45990.1 68416.m04976 actin-depolymerizing factor, putative s... 87 1e-17 At5g59880.2 68418.m07509 actin-depolymerizing factor 3 (ADF3) id... 74 1e-13 At3g03290.1 68416.m00326 universal stress protein (USP) family p... 31 0.58 At3g15070.1 68416.m01906 zinc finger (C3HC4-type RING finger) fa... 27 9.5 At1g64430.1 68414.m07302 expressed protein 27 9.5 >At5g59890.1 68418.m07510 actin-depolymerizing factor 4 (ADF4) identical to SP|Q9ZSK3 Actin-depolymerizing factor 4 (ADF-4) (AtADF4) {Arabidopsis thaliana} Length = 139 Score = 115 bits (276), Expect = 4e-26 Identities = 57/142 (40%), Positives = 88/142 (61%), Gaps = 1/142 (0%) Frame = +3 Query: 54 ASGVTVSDACKTTYEEIKKDKKHRYVVFYIRD-EKQIDVETVGERNAEYEQFLEDLQKGG 230 ASG+ V D CK + E+K + HR++V+ I + +KQ+ VE VGE YE F L Sbjct: 5 ASGMAVHDDCKLRFLELKAKRTHRFIVYKIEEKQKQVIVEKVGEPILTYEDFAASLP--- 61 Query: 231 TGECRYGLFDFEYTHQCQGTSEASKKQKLFLMSWCPDTAKVKKKMLYSSSFDALKKSLVG 410 ECRY ++DF++ T+E +K K+F ++WCPD AKV+ KM+Y+SS D K+ L G Sbjct: 62 ADECRYAIYDFDFV-----TAENCQKSKIFFIAWCPDVAKVRSKMIYASSKDRFKRELDG 116 Query: 411 VQKYIQATDLSEASHEAVEXKL 476 +Q +QATD +E + ++ ++ Sbjct: 117 IQVELQATDPTEMDLDVLKSRV 138 >At3g46010.1 68416.m04978 actin-depolymerizing factor 1 (ADF1) identical to SP|Q39250 Actin-depolymerizing factor 1 (ADF-1) (AtADF1) {Arabidopsis thaliana} Length = 139 Score = 112 bits (269), Expect = 3e-25 Identities = 56/132 (42%), Positives = 84/132 (63%), Gaps = 1/132 (0%) Frame = +3 Query: 54 ASGVTVSDACKTTYEEIKKDKKHRYVVFYIRD-EKQIDVETVGERNAEYEQFLEDLQKGG 230 ASG+ V D CK + E+K + HR++V+ I + +KQ+ VE VG+ YE+F L Sbjct: 5 ASGMAVHDDCKLRFLELKAKRTHRFIVYKIEEKQKQVVVEKVGQPIQTYEEFAACLP--- 61 Query: 231 TGECRYGLFDFEYTHQCQGTSEASKKQKLFLMSWCPDTAKVKKKMLYSSSFDALKKSLVG 410 ECRY ++DF++ T+E +K K+F ++WCPD AKV+ KM+Y+SS D K+ L G Sbjct: 62 ADECRYAIYDFDFV-----TAENCQKSKIFFIAWCPDIAKVRSKMIYASSKDRFKRELDG 116 Query: 411 VQKYIQATDLSE 446 +Q +QATD +E Sbjct: 117 IQVELQATDPTE 128 >At5g59890.2 68418.m07511 actin-depolymerizing factor 4 (ADF4) identical to SP|Q9ZSK3 Actin-depolymerizing factor 4 (ADF-4) (AtADF4) {Arabidopsis thaliana} Length = 132 Score = 109 bits (262), Expect = 2e-24 Identities = 54/139 (38%), Positives = 85/139 (61%), Gaps = 1/139 (0%) Frame = +3 Query: 63 VTVSDACKTTYEEIKKDKKHRYVVFYIRD-EKQIDVETVGERNAEYEQFLEDLQKGGTGE 239 + V D CK + E+K + HR++V+ I + +KQ+ VE VGE YE F L E Sbjct: 1 MAVHDDCKLRFLELKAKRTHRFIVYKIEEKQKQVIVEKVGEPILTYEDFAASLP---ADE 57 Query: 240 CRYGLFDFEYTHQCQGTSEASKKQKLFLMSWCPDTAKVKKKMLYSSSFDALKKSLVGVQK 419 CRY ++DF++ T+E +K K+F ++WCPD AKV+ KM+Y+SS D K+ L G+Q Sbjct: 58 CRYAIYDFDFV-----TAENCQKSKIFFIAWCPDVAKVRSKMIYASSKDRFKRELDGIQV 112 Query: 420 YIQATDLSEASHEAVEXKL 476 +QATD +E + ++ ++ Sbjct: 113 ELQATDPTEMDLDVLKSRV 131 >At4g00680.1 68417.m00093 actin-depolymerizing factor, putative strong similarity to SP|P30175 Actin-depolymerizing factor (ADF) {Lilium longiflorum}; contains Pfam profile PF00241: Cofilin/tropomyosin-type actin-binding protein Length = 140 Score = 109 bits (261), Expect = 2e-24 Identities = 55/143 (38%), Positives = 89/143 (62%), Gaps = 2/143 (1%) Frame = +3 Query: 54 ASGVTVSDACKTTYEEIKKDKKHRYVVFYIRDEK--QIDVETVGERNAEYEQFLEDLQKG 227 ASG+ V+D CK + E+K + +R++VF I DEK Q+ +E +G Y+ F + Sbjct: 5 ASGMHVNDECKIKFLELKAKRTYRFIVFKI-DEKAQQVQIEKLGNPEETYDDFTSSIPDD 63 Query: 228 GTGECRYGLFDFEYTHQCQGTSEASKKQKLFLMSWCPDTAKVKKKMLYSSSFDALKKSLV 407 ECRY ++DF++T T + +K K+F ++W PDT++V+ KMLY+SS D K+ + Sbjct: 64 ---ECRYAVYDFDFT-----TEDNCQKSKIFFIAWSPDTSRVRSKMLYASSKDRFKREME 115 Query: 408 GVQKYIQATDLSEASHEAVEXKL 476 G+Q +QATD SE S + ++ +L Sbjct: 116 GIQVELQATDPSEMSLDIIKGRL 138 >At1g01750.1 68414.m00094 actin-depolymerizing factor, putative strong similarity to SP|P30175 Actin-depolymerizing factor (ADF) {Lilium longiflorum}; contains Pfam profile PF00241: Cofilin/tropomyosin-type actin-binding protein Length = 140 Score = 109 bits (261), Expect = 2e-24 Identities = 56/143 (39%), Positives = 90/143 (62%), Gaps = 2/143 (1%) Frame = +3 Query: 54 ASGVTVSDACKTTYEEIKKDKKHRYVVFYIRDEK--QIDVETVGERNAEYEQFLEDLQKG 227 ASG+ VSD CK + E+K + +R++VF I DEK Q+ ++ +G YE F + + Sbjct: 5 ASGMHVSDECKLKFLELKAKRNYRFIVFKI-DEKAQQVMIDKLGNPEETYEDFTRSIPED 63 Query: 228 GTGECRYGLFDFEYTHQCQGTSEASKKQKLFLMSWCPDTAKVKKKMLYSSSFDALKKSLV 407 ECRY ++D+++T T E +K K+F ++W PDT++V+ KMLY+SS D K+ L Sbjct: 64 ---ECRYAVYDYDFT-----TPENCQKSKIFFIAWSPDTSRVRSKMLYASSKDRFKRELD 115 Query: 408 GVQKYIQATDLSEASHEAVEXKL 476 G+Q +QATD SE S + ++ ++ Sbjct: 116 GIQVELQATDPSEMSLDIIKGRV 138 >At5g52360.1 68418.m06497 actin-depolymerizing factor, putative strong similarity to pollen specific actin-depolymerizing factor 2 [Nicotiana tabacum] GI:22857914; contains Pfam profile PF00241: Cofilin/tropomyosin-type actin-binding protein Length = 137 Score = 106 bits (255), Expect = 1e-23 Identities = 53/140 (37%), Positives = 87/140 (62%) Frame = +3 Query: 54 ASGVTVSDACKTTYEEIKKDKKHRYVVFYIRDEKQIDVETVGERNAEYEQFLEDLQKGGT 233 ASG+ V D CK + E+K + +R+++F I D +Q+ VE +G Y+ F L Sbjct: 5 ASGMAVEDECKLKFLELKAKRNYRFIIFRI-DGQQVVVEKLGSPQENYDDFTNYLPPN-- 61 Query: 234 GECRYGLFDFEYTHQCQGTSEASKKQKLFLMSWCPDTAKVKKKMLYSSSFDALKKSLVGV 413 ECRY ++DF++T T+E +K K+F ++W PD+++V+ KM+Y+SS D K+ L G+ Sbjct: 62 -ECRYAVYDFDFT-----TAENIQKSKIFFIAWSPDSSRVRMKMVYASSKDRFKRELDGI 115 Query: 414 QKYIQATDLSEASHEAVEXK 473 Q +QATD SE S + ++ + Sbjct: 116 QVELQATDPSEMSLDIIKSR 135 >At5g59880.1 68418.m07508 actin-depolymerizing factor 3 (ADF3) identical to SP|Q9ZSK4 Actin-depolymerizing factor 3 (ADF 3) (AtADF3) {Arabidopsis thaliana} Length = 139 Score = 105 bits (252), Expect = 3e-23 Identities = 51/132 (38%), Positives = 81/132 (61%), Gaps = 1/132 (0%) Frame = +3 Query: 54 ASGVTVSDACKTTYEEIKKDKKHRYVVFYIRD-EKQIDVETVGERNAEYEQFLEDLQKGG 230 ASG+ V D CK + E+K + HR++++ I + +KQ+ VE +GE +E L Sbjct: 5 ASGMAVHDDCKLKFMELKTKRTHRFIIYKIEELQKQVIVEKIGEPGQTHEDLAASLP--- 61 Query: 231 TGECRYGLFDFEYTHQCQGTSEASKKQKLFLMSWCPDTAKVKKKMLYSSSFDALKKSLVG 410 ECRY +FDF++ +SE + ++F ++W PDTA+V+ KM+Y+SS D K+ L G Sbjct: 62 ADECRYAIFDFDFV-----SSEGVPRSRIFFVAWSPDTARVRSKMIYASSKDRFKRELDG 116 Query: 411 VQKYIQATDLSE 446 +Q +QATD +E Sbjct: 117 IQVELQATDPTE 128 >At3g46000.1 68416.m04977 actin-depolymerizing factor, putative (ADF2) strong similarity to SP|Q9ZSK3 Actin-depolymerizing factor 4 (ADF-4) (AtADF4) {Arabidopsis thaliana}; contains Pfam profile PF00241: Cofilin/tropomyosin-type actin-binding protein Length = 137 Score = 104 bits (249), Expect = 7e-23 Identities = 54/131 (41%), Positives = 80/131 (61%) Frame = +3 Query: 54 ASGVTVSDACKTTYEEIKKDKKHRYVVFYIRDEKQIDVETVGERNAEYEQFLEDLQKGGT 233 ASG+ V D CK + E+K + R +V+ I D KQ+ VE +GE Y+ F L Sbjct: 5 ASGMAVHDDCKLKFMELKAKRTFRTIVYKIED-KQVIVEKLGEPEQSYDDFAASLP---A 60 Query: 234 GECRYGLFDFEYTHQCQGTSEASKKQKLFLMSWCPDTAKVKKKMLYSSSFDALKKSLVGV 413 +CRY ++DF++ T+E +K K+F ++W PDTAKV+ KM+Y+SS D K+ L G+ Sbjct: 61 DDCRYCIYDFDFV-----TAENCQKSKIFFIAWSPDTAKVRDKMIYASSKDRFKRELDGI 115 Query: 414 QKYIQATDLSE 446 Q +QATD +E Sbjct: 116 QVELQATDPTE 126 >At2g31200.1 68415.m03810 actin-depolymerizing factor 6 (ADF6) identical to SP|Q9ZSK2 Actin-depolymerizing factor 6 (ADF-6) (AtADF6) {Arabidopsis thaliana} Length = 146 Score = 103 bits (248), Expect = 9e-23 Identities = 54/140 (38%), Positives = 83/140 (59%), Gaps = 1/140 (0%) Frame = +3 Query: 57 SGVTVSDACKTTYEEIKKDKKHRYVVFYIRD-EKQIDVETVGERNAEYEQFLEDLQKGGT 233 SG+ V+D KTT+ E+++ K HRYVVF I + +K++ VE G Y+ FL L Sbjct: 13 SGMGVADESKTTFLELQRKKTHRYVVFKIDESKKEVVVEKTGNPTESYDDFLASLPDN-- 70 Query: 234 GECRYGLFDFEYTHQCQGTSEASKKQKLFLMSWCPDTAKVKKKMLYSSSFDALKKSLVGV 413 +CRY ++DF++ TSE +K K+F +W P T+ ++ K+LYS+S D L + L G+ Sbjct: 71 -DCRYAVYDFDFV-----TSENCQKSKIFFFAWSPSTSGIRAKVLYSTSKDQLSRELQGI 124 Query: 414 QKYIQATDLSEASHEAVEXK 473 IQATD +E E + + Sbjct: 125 HYEIQATDPTEVDLEVLRER 144 >At4g25590.1 68417.m03687 actin-depolymerizing factor, putative strong similarity to pollen specific actin-depolymerizing factor 2 [Nicotiana tabacum] GI:22857914; contains Pfam profile PF00241: Cofilin/tropomyosin-type actin-binding protein Length = 130 Score = 102 bits (244), Expect = 3e-22 Identities = 50/137 (36%), Positives = 83/137 (60%) Frame = +3 Query: 63 VTVSDACKTTYEEIKKDKKHRYVVFYIRDEKQIDVETVGERNAEYEQFLEDLQKGGTGEC 242 + V D CK + E+K + +R+++F I D +Q+ VE +G + Y+ F L EC Sbjct: 1 MAVEDECKLKFLELKSKRNYRFIIFRI-DGQQVVVEKLGNPDETYDDFTASLP---ANEC 56 Query: 243 RYGLFDFEYTHQCQGTSEASKKQKLFLMSWCPDTAKVKKKMLYSSSFDALKKSLVGVQKY 422 RY +FDF++ T E +K K+F ++W PD+++V+ KM+Y+SS D K+ L G+Q Sbjct: 57 RYAVFDFDFI-----TDENCQKSKIFFIAWSPDSSRVRMKMVYASSKDRFKRELDGIQVE 111 Query: 423 IQATDLSEASHEAVEXK 473 +QATD SE S + ++ + Sbjct: 112 LQATDPSEMSFDIIKSR 128 >At2g16700.1 68415.m01916 actin-depolymerizing factor 5 (ADF5) identical to SP|Q9ZNT3 Actin-depolymerizing factor 5 (ADF-5) (AtADF5) {Arabidopsis thaliana} Length = 143 Score = 94.7 bits (225), Expect = 6e-20 Identities = 46/146 (31%), Positives = 88/146 (60%), Gaps = 2/146 (1%) Frame = +3 Query: 48 KMAS-GVTVSDACKTTYEEIKKDKKHRYVVFYIRDE-KQIDVETVGERNAEYEQFLEDLQ 221 KMA+ G+ V+D C +++ ++K K HRY+VF I ++ +++ V+ VG Y + L Sbjct: 6 KMATTGMRVTDECTSSFMDMKWKKVHRYIVFKIEEKSRKVTVDKVGGAGESYHDLEDSLP 65 Query: 222 KGGTGECRYGLFDFEYTHQCQGTSEASKKQKLFLMSWCPDTAKVKKKMLYSSSFDALKKS 401 +CRY +FDF++ T + +K K+F ++W P+ +K++ K+LY++S D L++ Sbjct: 66 ---VDDCRYAVFDFDFV-----TVDNCRKSKIFFIAWSPEASKIRAKILYATSKDGLRRV 117 Query: 402 LVGVQKYIQATDLSEASHEAVEXKLR 479 L G+ +QATD +E + ++ + + Sbjct: 118 LEGIHYELQATDPTEMGFDIIQDRAK 143 >At4g34970.1 68417.m04957 actin-depolymerizing factor, putative similar to SP|Q9ZNT3 Actin-depolymerizing factor 5 (ADF-5) (AtADF5) {Arabidopsis thaliana}; contains Pfam profile PF00241: Cofilin/tropomyosin-type actin-binding protein Length = 130 Score = 88.6 bits (210), Expect = 4e-18 Identities = 41/138 (29%), Positives = 82/138 (59%), Gaps = 1/138 (0%) Frame = +3 Query: 69 VSDACKTTYEEIKKDKKHRYVVFYIRDE-KQIDVETVGERNAEYEQFLEDLQKGGTGECR 245 ++D CK ++ E+K K HRYVV+ + ++ +++ V+ VG Y+ L + +CR Sbjct: 1 MTDDCKKSFMEMKWKKVHRYVVYKLEEKSRKVTVDKVGAAGESYDDLAASLPED---DCR 57 Query: 246 YGLFDFEYTHQCQGTSEASKKQKLFLMSWCPDTAKVKKKMLYSSSFDALKKSLVGVQKYI 425 Y +FDF+Y T + + K+F ++W P+ +++++KM+Y++S L++ L GV + Sbjct: 58 YAVFDFDYV-----TVDNCRMSKIFFITWSPEASRIREKMMYATSKSGLRRVLDGVHYEL 112 Query: 426 QATDLSEASHEAVEXKLR 479 QATD +E + ++ + + Sbjct: 113 QATDPTEMGFDKIQDRAK 130 >At3g45990.1 68416.m04976 actin-depolymerizing factor, putative similar to SP|Q9ZSK3 Actin-depolymerizing factor 4 (ADF-4) (AtADF4) {Arabidopsis thaliana}; contains Pfam profile PF00241: Cofilin/tropomyosin-type actin-binding protein Length = 133 Score = 87.0 bits (206), Expect = 1e-17 Identities = 49/144 (34%), Positives = 78/144 (54%), Gaps = 6/144 (4%) Frame = +3 Query: 63 VTVSDACKTTYEEIKKDKKHRYVVFYIRDEKQIDVETV------GERNAEYEQFLEDLQK 224 + + D CK T+ E+K+ + R +V+ I D Q+ VE GER YE+F L Sbjct: 1 MVLHDDCKLTFLELKERRTFRSIVYKIEDNMQVIVEKHHYKKMHGEREQSYEEFANSLP- 59 Query: 225 GGTGECRYGLFDFEYTHQCQGTSEASKKQKLFLMSWCPDTAKVKKKMLYSSSFDALKKSL 404 ECRY + D E+ ++K+ ++W P TAK++KKM+YSS+ D K+ L Sbjct: 60 --ADECRYAILDIEFV---------PGERKICFIAWSPSTAKMRKKMIYSSTKDRFKREL 108 Query: 405 VGVQKYIQATDLSEASHEAVEXKL 476 G+Q ATDL++ S +A+ ++ Sbjct: 109 DGIQVEFHATDLTDISLDAIRRRI 132 >At5g59880.2 68418.m07509 actin-depolymerizing factor 3 (ADF3) identical to SP|Q9ZSK4 Actin-depolymerizing factor 3 (ADF 3) (AtADF3) {Arabidopsis thaliana} Length = 124 Score = 73.7 bits (173), Expect = 1e-13 Identities = 35/100 (35%), Positives = 58/100 (58%), Gaps = 1/100 (1%) Frame = +3 Query: 54 ASGVTVSDACKTTYEEIKKDKKHRYVVFYIRD-EKQIDVETVGERNAEYEQFLEDLQKGG 230 ASG+ V D CK + E+K + HR++++ I + +KQ+ VE +GE +E L Sbjct: 5 ASGMAVHDDCKLKFMELKTKRTHRFIIYKIEELQKQVIVEKIGEPGQTHEDLAASLP--- 61 Query: 231 TGECRYGLFDFEYTHQCQGTSEASKKQKLFLMSWCPDTAK 350 ECRY +FDF++ +SE + ++F ++W PDTA+ Sbjct: 62 ADECRYAIFDFDFV-----SSEGVPRSRIFFVAWSPDTAR 96 >At3g03290.1 68416.m00326 universal stress protein (USP) family protein contains Pfam profile: PF00582 universal stress protein family Length = 274 Score = 31.5 bits (68), Expect = 0.58 Identities = 12/24 (50%), Positives = 18/24 (75%) Frame = +3 Query: 168 ETVGERNAEYEQFLEDLQKGGTGE 239 ET G+ +EYE+F E+ ++GG GE Sbjct: 45 ETDGDSTSEYEEFEEEKERGGEGE 68 >At3g15070.1 68416.m01906 zinc finger (C3HC4-type RING finger) family protein similar to C-terminal zinc-finger [Glycine max] GI:558543; contains Pfam profile: PF00097 zinc finger, C3HC4 type (RING finger) Length = 486 Score = 27.5 bits (58), Expect = 9.5 Identities = 14/48 (29%), Positives = 23/48 (47%), Gaps = 3/48 (6%) Frame = -2 Query: 342 CPGTTTSGRASVSC*PPTCP---GTGACIQSQTGHICIPRYRPSADPR 208 C G + G +S+ P P G A ++G++C PRY ++ R Sbjct: 217 CNGNRSDGSSSLWSQAPAVPYMHGNIATGSIESGNVCFPRYHETSSSR 264 >At1g64430.1 68414.m07302 expressed protein Length = 559 Score = 27.5 bits (58), Expect = 9.5 Identities = 13/30 (43%), Positives = 17/30 (56%) Frame = -3 Query: 563 CRQGRSVGERVRFRRELVCKCCLLAIGGAE 474 CR G S E V F + + C CL+AI A+ Sbjct: 180 CRVGISPAEEVPFGKIVRCPSCLIAIAVAQ 209 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 13,534,630 Number of Sequences: 28952 Number of extensions: 253386 Number of successful extensions: 834 Number of sequences better than 10.0: 17 Number of HSP's better than 10.0 without gapping: 771 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 801 length of database: 12,070,560 effective HSP length: 79 effective length of database: 9,783,352 effective search space used: 1575119672 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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