Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= brP-0066
(700 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At4g32190.1 68417.m04581 centromeric protein-related low similar... 30 1.7
At3g45940.1 68416.m04971 alpha-xylosidase, putative strong simil... 29 2.2
At2g44930.1 68415.m05593 expressed protein contains Pfam profile... 29 2.2
At4g19865.1 68417.m02911 kelch repeat-containing F-box family pr... 29 3.9
At4g22760.1 68417.m03286 pentatricopeptide (PPR) repeat-containi... 28 5.2
At4g04710.1 68417.m00692 calcium-dependent protein kinase, putat... 28 5.2
At3g43590.1 68416.m04638 zinc knuckle (CCHC-type) family protein... 28 5.2
At3g19190.1 68416.m02436 expressed protein 28 5.2
At1g18960.1 68414.m02359 myb family transcription factor contain... 28 5.2
At5g67100.1 68418.m08460 DNA-directed DNA polymerase alpha catal... 28 6.8
At3g05680.1 68416.m00634 expressed protein 28 6.8
At5g13520.1 68418.m01561 peptidase M1 family protein similar to ... 27 9.0
At3g11300.1 68416.m01374 hypothetical protein 27 9.0
At2g39120.1 68415.m04806 expressed protein 27 9.0
>At4g32190.1 68417.m04581 centromeric protein-related low similarity
to SP|Q02224 Centromeric protein E (CENP-E protein)
{Homo sapiens}
Length = 783
Score = 29.9 bits (64), Expect = 1.7
Identities = 21/53 (39%), Positives = 34/53 (64%), Gaps = 2/53 (3%)
Frame = +2
Query: 164 ERKRDKPILELSKHSKKFDISSLQDN-YLKYDP-SVPQNLQIVEMDSNILDLK 316
E +++KP+LEL+ H DIS +QD Y K + V QNL + E +S++++ K
Sbjct: 421 ELQKEKPLLELAMH----DISVIQDELYKKANAFQVSQNL-LQEKESSLVEAK 468
>At3g45940.1 68416.m04971 alpha-xylosidase, putative strong
similarity to alpha-xylosidase precursor GI:4163997 from
[Arabidopsis thaliana]
Length = 868
Score = 29.5 bits (63), Expect = 2.2
Identities = 16/60 (26%), Positives = 25/60 (41%)
Frame = +2
Query: 122 IHIATPIPLHIALRERKRDKPILELSKHSKKFDISSLQDNYLKYDPSVPQNLQIVEMDSN 301
I I T P A+R R + I S + F +D YL+ S+P++ + N
Sbjct: 121 ILIFTVDPFSFAVRRRSNGETIFNTSSSDESFGEMVFKDQYLEISTSLPKDASLYGFGEN 180
>At2g44930.1 68415.m05593 expressed protein contains Pfam profile
PF03140: Plant protein of unknown function
Length = 515
Score = 29.5 bits (63), Expect = 2.2
Identities = 23/86 (26%), Positives = 41/86 (47%), Gaps = 3/86 (3%)
Frame = +2
Query: 95 VPHEARQLSIHIATPIPLHIALRERKRDKPILELSKHSKKF-DISSLQDNYLKYDPSVPQ 271
VP+ R+++ TP L I + + LELSK ++ D +++ + KY ++
Sbjct: 89 VPNRLRRVNPEAYTPQMLLIGPLQHSKKANALELSKTDLRYLDYMNMELHKKKYLNAIAN 148
Query: 272 NL--QIVEMDSNILDLKESFRYEGEA 343
QI+E+ I++ E F E A
Sbjct: 149 KYGDQIIEVFKEIIETSEKFVRESYA 174
>At4g19865.1 68417.m02911 kelch repeat-containing F-box family
protein contains F-box domain Pfam:PF00646 and Kelch
motif Pfam:PF01344
Length = 393
Score = 28.7 bits (61), Expect = 3.9
Identities = 12/25 (48%), Positives = 15/25 (60%)
Frame = -3
Query: 155 QCGGESVWLCVWTVDVLRAAPSSCE 81
+C G VW V +VDVL P SC+
Sbjct: 360 KCDGGGVWGIVESVDVLYTVPISCQ 384
>At4g22760.1 68417.m03286 pentatricopeptide (PPR) repeat-containing
protein contains Pfam profile PF01535: PPR repeat
Length = 889
Score = 28.3 bits (60), Expect = 5.2
Identities = 11/20 (55%), Positives = 13/20 (65%)
Frame = +1
Query: 451 SYIRRCGQCGRVSQLQHSNW 510
S I+RC QC + LQH NW
Sbjct: 849 SRIKRCLQCQPATTLQHVNW 868
>At4g04710.1 68417.m00692 calcium-dependent protein kinase, putative
/ CDPK, putative similar to calcium-dependent protein
kinase [Nicotiana tabacum] gi|3283996|gb|AAC25423;
contains protein kinase domain, Pfam:PF00069; contains
EF hand domain (calcium-binding EF-hand), Pfam:PF00036,
INTERPRO:IPR002048
Length = 575
Score = 28.3 bits (60), Expect = 5.2
Identities = 10/30 (33%), Positives = 21/30 (70%)
Frame = +3
Query: 594 QPSVIEIRNVHRLDTNHAIHTVVNLCGASD 683
QP++++I+ + + N++IH V+ LCG +
Sbjct: 80 QPNIVQIKGSY--EDNNSIHIVMELCGGGE 107
>At3g43590.1 68416.m04638 zinc knuckle (CCHC-type) family protein
contains Pfam domain, PF00098: Zinc knuckle
Length = 551
Score = 28.3 bits (60), Expect = 5.2
Identities = 16/47 (34%), Positives = 20/47 (42%)
Frame = +2
Query: 485 SVNYSTPTGRTRPLEYRWSIERGPCSASCGGGVRLITAQCHRDQKCP 625
S N TPT R +P S+E G S G + HR + CP
Sbjct: 178 SFNCPTPTKRRKPCFICGSLEHGAKQCSKGHDCYICKKTGHRAKDCP 224
>At3g19190.1 68416.m02436 expressed protein
Length = 1861
Score = 28.3 bits (60), Expect = 5.2
Identities = 18/51 (35%), Positives = 26/51 (50%), Gaps = 3/51 (5%)
Frame = +2
Query: 446 VNPTLEDAVSVDVSVNYSTPTGRTRPLEYRW--SIERGPCSAS-CGGGVRL 589
VNPT + SV+ NY +P G + Y W + E+ P + S CG R+
Sbjct: 1280 VNPTYQSD-SVECQNNYMSPHGISHGQAYNWVPATEKLPSNQSICGSSSRI 1329
>At1g18960.1 68414.m02359 myb family transcription factor contains
Pfam profile: PF00249 myb-like DNA-binding domain;
contains similarity to transcription factor GI:9759592
from [Arabidopsis thaliana]
Length = 307
Score = 28.3 bits (60), Expect = 5.2
Identities = 15/57 (26%), Positives = 26/57 (45%), Gaps = 2/57 (3%)
Frame = +1
Query: 457 IRRCGQCGRVSQLQHSNWTHETIGIQVVDRARSVFRVL--RWRCAIDNSPVSSRSEM 621
+RRCG+ RV + HS HE+ + D + + RW+ + P + E+
Sbjct: 41 LRRCGKSCRVRKTDHSGTKHESFTSEDEDLIIKMHAAMGSRWQLIAQHLPGKTEEEV 97
>At5g67100.1 68418.m08460 DNA-directed DNA polymerase alpha catalytic
subunit, putative similar to SP|O48653 DNA polymerase
alpha catalytic subunit (EC 2.7.7.7) {Oryza sativa};
contains Pfam profiles: PF03175 DNA polymerase type B,
organellar and viral, PF00136 DNA polymerase family B,
PF03104 DNA polymerase family B, exonuclease domain
Length = 1492
Score = 27.9 bits (59), Expect = 6.8
Identities = 16/37 (43%), Positives = 20/37 (54%), Gaps = 1/37 (2%)
Frame = -3
Query: 377 FDSSAEESQ-P*SPRLHNESSLSSLKCYCPFPQSEDS 270
F +S EES+ P +P S LK +CP Q EDS
Sbjct: 1315 FATSDEESKKPATPETEESDSTFWLKLHCPKCQQEDS 1351
>At3g05680.1 68416.m00634 expressed protein
Length = 2057
Score = 27.9 bits (59), Expect = 6.8
Identities = 25/88 (28%), Positives = 46/88 (52%), Gaps = 9/88 (10%)
Frame = +2
Query: 239 NYLKYDPSVPQNLQIVEMDSNILDLKESFRYEGEAITAGTLLR----WN-----QTDTDI 391
N Y PS P L++ + +N L ++ S+R I G +++ +N ++ TDI
Sbjct: 41 NPFLYTPSAPYPLEVEAVVTNHLVVRGSYR-SLSLIVYGNIVKDLGQYNIILEGRSVTDI 99
Query: 392 YITSESRLQTDLMIMAIPVNPTLEDAVS 475
++E L+ DL ++ VN T+E+ +S
Sbjct: 100 VSSTEGNLE-DLPLVLHSVNRTIEECLS 126
>At5g13520.1 68418.m01561 peptidase M1 family protein similar to
SP|P09960 Leukotriene A-4 hydrolase (EC 3.3.2.6) (LTA-4
hydrolase) {Homo sapiens}; contains Pfam profile
PF01433: Peptidase family M1
Length = 616
Score = 27.5 bits (58), Expect = 9.0
Identities = 15/85 (17%), Positives = 40/85 (47%)
Frame = +2
Query: 296 SNILDLKESFRYEGEAITAGTLLRWNQTDTDIYITSESRLQTDLMIMAIPVNPTLEDAVS 475
+ I+ L + F+ EG+ + + WN + ++Y+ + + +MA+ L ++
Sbjct: 476 TKIISLAKEFK-EGKMPSEDDVAEWNGQEWELYLENLPKSCEPSQVMALDKRYRLAESKD 534
Query: 476 VDVSVNYSTPTGRTRPLEYRWSIER 550
+V V++ ++ EY +++
Sbjct: 535 YEVKVSFLQLAVTSKCREYHGEVKK 559
>At3g11300.1 68416.m01374 hypothetical protein
Length = 208
Score = 27.5 bits (58), Expect = 9.0
Identities = 17/71 (23%), Positives = 36/71 (50%), Gaps = 2/71 (2%)
Frame = -1
Query: 352 SRDRLAFITKALFQV*NVTVHF--HNLKILRHARIVFQVVVLKGADVEFFGVLRKF*NRL 179
+RDR+A ++ L V +++ F H+ ++ + + ++ +G + F G + R
Sbjct: 125 TRDRMASCSRILLLVASLSAIFDEHSSLQVQCETLFYGRLIYRGLEQRFGGTIVILPKRC 184
Query: 178 VPFPFAQCNVE 146
VP+PF + E
Sbjct: 185 VPYPFPPPHTE 195
>At2g39120.1 68415.m04806 expressed protein
Length = 387
Score = 27.5 bits (58), Expect = 9.0
Identities = 12/36 (33%), Positives = 24/36 (66%)
Frame = +2
Query: 158 LRERKRDKPILELSKHSKKFDISSLQDNYLKYDPSV 265
++E R PI +SK +++FD+S+ ++L+ PS+
Sbjct: 60 VQEPNRCIPISAISKKTRQFDVSTKIAHFLRKFPSI 95
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 14,972,592
Number of Sequences: 28952
Number of extensions: 312581
Number of successful extensions: 919
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 893
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 918
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1496852856
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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