BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= brP-0054 (605 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC6G10.11c |ubi3||ribosomal ubiquitin fusion protein Ubi3|Schi... 175 3e-45 SPAC589.10c |||ribomal-ubiquitin fusion protein Ubi5|Schizosacch... 175 3e-45 SPAC1805.12c |uep1|ubi2|ribosomal-ubiquitin fusion protein Ubi2|... 116 4e-27 SPAC11G7.04 |ubi1||ribosomal-ubiquitin fusion protein Ubi1|Schiz... 116 4e-27 SPBC337.08c |ubi4||ubiquitin|Schizosaccharomyces pombe|chr 2|||M... 116 4e-27 SPBC12D12.08c |ned8|nedd8, ubl1, SPBC24C6.01c|ubiquitin-like pro... 71 1e-13 SPAC6G9.08 |ubp6||ubiquitin C-terminal hydrolase Ubp6|Schizosacc... 40 3e-04 SPBC2D10.12 |rhp23||Rad23 homolog Rhp23|Schizosaccharomyces pomb... 40 4e-04 SPAC26A3.16 |dph1|ucp5|UBA domain protein Dph1|Schizosaccharomyc... 38 0.001 SPAC227.15 |||protein phosphatase regulatory subunit Reg1 |Schiz... 31 0.13 SPBC1718.06 |msp1|mgm1|mitochondrial GTPase Msp1|Schizosaccharom... 27 2.8 SPAC29B12.06c |rcd1||RNA-binding protein Rcd1 |Schizosaccharomyc... 27 2.8 SPBC31E1.03 |hub1|ubl4|ubiquitin-like protein modifier Hub1|Schi... 25 6.5 SPAC688.10 |rev3||DNA polymerase zeta catalytic subunit Rev3|Sch... 25 8.6 >SPAC6G10.11c |ubi3||ribosomal ubiquitin fusion protein Ubi3|Schizosaccharomyces pombe|chr 1|||Manual Length = 150 Score = 175 bits (427), Expect = 3e-45 Identities = 86/137 (62%), Positives = 99/137 (72%) Frame = +2 Query: 47 TITLEVEPSDTIENVKAKIQDKEGIPPNQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVX 226 TITLEVE SDTI+NVK+KIQDKEGIPP+QQRLIFAGKQLEDGRTLSDYNIQKESTLHLV Sbjct: 12 TITLEVESSDTIDNVKSKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVL 71 Query: 227 XXXXXXXXXXXXNYSTPXXXXXXXXXXXLAVLRFYKVDENGKIHRLRRECTGEQCGAGVF 406 Y+TP LAVL++YKV+++G + RLRREC CGA F Sbjct: 72 RLRGGGKKRKKKTYTTPKKIKHKHKKVELAVLKYYKVEDDGSVKRLRREC--PNCGASTF 129 Query: 407 MAVMEDRHYCGKCHSTM 457 MA +DR YCG+CH T+ Sbjct: 130 MANHKDRLYCGRCHLTL 146 >SPAC589.10c |||ribomal-ubiquitin fusion protein Ubi5|Schizosaccharomyces pombe|chr 1|||Manual Length = 150 Score = 175 bits (427), Expect = 3e-45 Identities = 86/137 (62%), Positives = 99/137 (72%) Frame = +2 Query: 47 TITLEVEPSDTIENVKAKIQDKEGIPPNQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVX 226 TITLEVE SDTI+NVK+KIQDKEGIPP+QQRLIFAGKQLEDGRTLSDYNIQKESTLHLV Sbjct: 12 TITLEVESSDTIDNVKSKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVL 71 Query: 227 XXXXXXXXXXXXNYSTPXXXXXXXXXXXLAVLRFYKVDENGKIHRLRRECTGEQCGAGVF 406 Y+TP LAVL++YKV+++G + RLRREC CGA F Sbjct: 72 RLRGGGKKRKKKTYTTPKKIKHKHKKVELAVLKYYKVEDDGSVKRLRREC--PNCGASTF 129 Query: 407 MAVMEDRHYCGKCHSTM 457 MA +DR YCG+CH T+ Sbjct: 130 MANHKDRLYCGRCHLTL 146 >SPAC1805.12c |uep1|ubi2|ribosomal-ubiquitin fusion protein Ubi2|Schizosaccharomyces pombe|chr 1|||Manual Length = 128 Score = 116 bits (278), Expect = 4e-27 Identities = 55/59 (93%), Positives = 58/59 (98%) Frame = +2 Query: 47 TITLEVEPSDTIENVKAKIQDKEGIPPNQQRLIFAGKQLEDGRTLSDYNIQKESTLHLV 223 TITLEVE SDTI+NVK+KIQDKEGIPP+QQRLIFAGKQLEDGRTLSDYNIQKESTLHLV Sbjct: 12 TITLEVESSDTIDNVKSKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLV 70 >SPAC11G7.04 |ubi1||ribosomal-ubiquitin fusion protein Ubi1|Schizosaccharomyces pombe|chr 1|||Manual Length = 128 Score = 116 bits (278), Expect = 4e-27 Identities = 55/59 (93%), Positives = 58/59 (98%) Frame = +2 Query: 47 TITLEVEPSDTIENVKAKIQDKEGIPPNQQRLIFAGKQLEDGRTLSDYNIQKESTLHLV 223 TITLEVE SDTI+NVK+KIQDKEGIPP+QQRLIFAGKQLEDGRTLSDYNIQKESTLHLV Sbjct: 12 TITLEVESSDTIDNVKSKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLV 70 >SPBC337.08c |ubi4||ubiquitin|Schizosaccharomyces pombe|chr 2|||Manual Length = 382 Score = 116 bits (278), Expect = 4e-27 Identities = 55/59 (93%), Positives = 58/59 (98%) Frame = +2 Query: 47 TITLEVEPSDTIENVKAKIQDKEGIPPNQQRLIFAGKQLEDGRTLSDYNIQKESTLHLV 223 TITLEVE SDTI+NVK+KIQDKEGIPP+QQRLIFAGKQLEDGRTLSDYNIQKESTLHLV Sbjct: 12 TITLEVESSDTIDNVKSKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLV 70 Score = 116 bits (278), Expect = 4e-27 Identities = 55/59 (93%), Positives = 58/59 (98%) Frame = +2 Query: 47 TITLEVEPSDTIENVKAKIQDKEGIPPNQQRLIFAGKQLEDGRTLSDYNIQKESTLHLV 223 TITLEVE SDTI+NVK+KIQDKEGIPP+QQRLIFAGKQLEDGRTLSDYNIQKESTLHLV Sbjct: 88 TITLEVESSDTIDNVKSKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLV 146 Score = 116 bits (278), Expect = 4e-27 Identities = 55/59 (93%), Positives = 58/59 (98%) Frame = +2 Query: 47 TITLEVEPSDTIENVKAKIQDKEGIPPNQQRLIFAGKQLEDGRTLSDYNIQKESTLHLV 223 TITLEVE SDTI+NVK+KIQDKEGIPP+QQRLIFAGKQLEDGRTLSDYNIQKESTLHLV Sbjct: 164 TITLEVESSDTIDNVKSKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLV 222 Score = 116 bits (278), Expect = 4e-27 Identities = 55/59 (93%), Positives = 58/59 (98%) Frame = +2 Query: 47 TITLEVEPSDTIENVKAKIQDKEGIPPNQQRLIFAGKQLEDGRTLSDYNIQKESTLHLV 223 TITLEVE SDTI+NVK+KIQDKEGIPP+QQRLIFAGKQLEDGRTLSDYNIQKESTLHLV Sbjct: 240 TITLEVESSDTIDNVKSKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLV 298 Score = 116 bits (278), Expect = 4e-27 Identities = 55/59 (93%), Positives = 58/59 (98%) Frame = +2 Query: 47 TITLEVEPSDTIENVKAKIQDKEGIPPNQQRLIFAGKQLEDGRTLSDYNIQKESTLHLV 223 TITLEVE SDTI+NVK+KIQDKEGIPP+QQRLIFAGKQLEDGRTLSDYNIQKESTLHLV Sbjct: 316 TITLEVESSDTIDNVKSKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLV 374 >SPBC12D12.08c |ned8|nedd8, ubl1, SPBC24C6.01c|ubiquitin-like protein modifier Ned8|Schizosaccharomyces pombe|chr 2|||Manual Length = 78 Score = 70.9 bits (166), Expect = 1e-13 Identities = 27/58 (46%), Positives = 44/58 (75%) Frame = +2 Query: 50 ITLEVEPSDTIENVKAKIQDKEGIPPNQQRLIFAGKQLEDGRTLSDYNIQKESTLHLV 223 I L+++P+D + +K ++++KEGIPP+QQRLI+AGKQ+ D + Y+++ S LHLV Sbjct: 13 IELDIDPNDKVSRIKERVEEKEGIPPSQQRLIYAGKQMADDKNAESYHLEGGSVLHLV 70 >SPAC6G9.08 |ubp6||ubiquitin C-terminal hydrolase Ubp6|Schizosaccharomyces pombe|chr 1|||Manual Length = 467 Score = 39.9 bits (89), Expect = 3e-04 Identities = 18/53 (33%), Positives = 31/53 (58%) Frame = +2 Query: 56 LEVEPSDTIENVKAKIQDKEGIPPNQQRLIFAGKQLEDGRTLSDYNIQKESTL 214 LE+EP++T +K ++ +PP +Q++I G QL+D L I+ +TL Sbjct: 15 LEIEPNETGSTLKHQLYSLTQVPPERQKVIVKGGQLKDDVLLGSVGIKPNATL 67 >SPBC2D10.12 |rhp23||Rad23 homolog Rhp23|Schizosaccharomyces pombe|chr 2|||Manual Length = 368 Score = 39.5 bits (88), Expect = 4e-04 Identities = 16/49 (32%), Positives = 31/49 (63%) Frame = +2 Query: 59 EVEPSDTIENVKAKIQDKEGIPPNQQRLIFAGKQLEDGRTLSDYNIQKE 205 +V I +K KIQ ++ +Q+LI++G+ L D +T+ +YNI+++ Sbjct: 17 DVSADTKISELKEKIQTQQNYEVERQKLIYSGRILADDKTVGEYNIKEQ 65 >SPAC26A3.16 |dph1|ucp5|UBA domain protein Dph1|Schizosaccharomyces pombe|chr 1|||Manual Length = 354 Score = 37.9 bits (84), Expect = 0.001 Identities = 19/54 (35%), Positives = 30/54 (55%) Frame = +2 Query: 62 VEPSDTIENVKAKIQDKEGIPPNQQRLIFAGKQLEDGRTLSDYNIQKESTLHLV 223 V+ ++ +K I I +QRLI+AG+ L+D +L Y IQ ++HLV Sbjct: 20 VDSESSVLALKEAIAPVADIEKERQRLIYAGRVLKDEESLKTYKIQDGHSIHLV 73 >SPAC227.15 |||protein phosphatase regulatory subunit Reg1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 873 Score = 31.1 bits (67), Expect = 0.13 Identities = 15/35 (42%), Positives = 19/35 (54%) Frame = -2 Query: 535 NPSYTAVASVSYRLEGTYLXVVVFEDHRAVTLPAV 431 NP+YT SY L TYL F++HR + AV Sbjct: 144 NPAYTNSHQNSYSLNETYLSYDFFDNHRGASSSAV 178 >SPBC1718.06 |msp1|mgm1|mitochondrial GTPase Msp1|Schizosaccharomyces pombe|chr 2|||Manual Length = 903 Score = 26.6 bits (56), Expect = 2.8 Identities = 10/29 (34%), Positives = 17/29 (58%) Frame = -1 Query: 203 PSGYCSPRGYVHLPAAFQQRSTSVDWEGF 117 PS + PRG V+ P+ ++R + + GF Sbjct: 17 PSSFLKPRGLVYRPSQIRRRVSLLSLSGF 45 >SPAC29B12.06c |rcd1||RNA-binding protein Rcd1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 283 Score = 26.6 bits (56), Expect = 2.8 Identities = 14/54 (25%), Positives = 24/54 (44%), Gaps = 1/54 (1%) Frame = +2 Query: 317 VLRFY-KVDENGKIHRLRRECTGEQCGAGVFMAVMEDRHYCGKCHSTMVFKDDD 475 V+R Y ++ +N + R C E F V++D H KC + ++ D Sbjct: 224 VIRCYLRLSDNPRAREALRHCLPEPLRDATFAQVLKDDHNTKKCLAQLLINLSD 277 >SPBC31E1.03 |hub1|ubl4|ubiquitin-like protein modifier Hub1|Schizosaccharomyces pombe|chr 2|||Manual Length = 73 Score = 25.4 bits (53), Expect = 6.5 Identities = 14/57 (24%), Positives = 26/57 (45%) Frame = +2 Query: 50 ITLEVEPSDTIENVKAKIQDKEGIPPNQQRLIFAGKQLEDGRTLSDYNIQKESTLHL 220 + ++ P DT+ + K + + G P + L +D TL+DY I +L + Sbjct: 14 VRVKCMPDDTVGDFKKLVAAQTGTDPRRIVLKKWHSVFKDNITLADYEIHDGMSLEM 70 >SPAC688.10 |rev3||DNA polymerase zeta catalytic subunit Rev3|Schizosaccharomyces pombe|chr 1|||Manual Length = 1480 Score = 25.0 bits (52), Expect = 8.6 Identities = 12/24 (50%), Positives = 15/24 (62%) Frame = +2 Query: 125 PNQQRLIFAGKQLEDGRTLSDYNI 196 PNQ AGK+L+ R L DY+I Sbjct: 476 PNQGDTRKAGKRLKYSRNLDDYHI 499 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,167,036 Number of Sequences: 5004 Number of extensions: 39837 Number of successful extensions: 97 Number of sequences better than 10.0: 14 Number of HSP's better than 10.0 without gapping: 91 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 95 length of database: 2,362,478 effective HSP length: 69 effective length of database: 2,017,202 effective search space used: 266270664 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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