Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= brP-0043
(750 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At2g27600.1 68415.m03346 AAA-type ATPase family protein / vacuol... 66 2e-11
At1g08270.2 68414.m00912 expressed protein low similarity to SP|... 37 0.012
At1g08270.1 68414.m00913 expressed protein low similarity to SP|... 37 0.012
At3g27120.1 68416.m03393 spastin ATPase, putative similar to SWI... 31 0.82
At2g34560.2 68415.m04246 katanin, putative similar to katanin p6... 31 0.82
At2g34560.1 68415.m04245 katanin, putative similar to katanin p6... 31 0.82
At1g80350.1 68414.m09406 katanin 1 (KTN1) identical to katanin 1... 30 1.9
At4g04910.1 68417.m00714 AAA-type ATPase family protein similar ... 29 2.5
At2g26910.1 68415.m03228 ABC transporter family protein similar ... 29 3.3
At5g61510.1 68418.m07718 NADP-dependent oxidoreductase, putative... 29 4.4
At3g19740.1 68416.m02499 AAA-type ATPase family protein contains... 28 5.8
At1g64110.2 68414.m07264 AAA-type ATPase family protein contains... 28 5.8
At1g64110.1 68414.m07263 AAA-type ATPase family protein contains... 28 5.8
At1g50140.1 68414.m05623 AAA-type ATPase family protein contains... 28 5.8
At4g24860.1 68417.m03559 AAA-type ATPase family protein contains... 28 7.6
At4g02480.1 68417.m00335 AAA-type ATPase family protein contains... 28 7.6
At3g60160.1 68416.m06717 ABC transporter family protein similar ... 28 7.6
At1g02890.1 68414.m00256 AAA-type ATPase family protein contains... 28 7.6
>At2g27600.1 68415.m03346 AAA-type ATPase family protein / vacuolar
sorting protein-related similar to SP|P46467 SKD1
protein (Vacuolar sorting protein 4b) {Mus musculus};
contains Pfam profiles PF00004: ATPase AAA family,
PF04212: MIT domain
Length = 435
Score = 66.5 bits (155), Expect = 2e-11
Identities = 55/184 (29%), Positives = 74/184 (40%), Gaps = 6/184 (3%)
Frame = +1
Query: 160 SNTLQKAIDLVTKATEEDKNKNYEEALRLYEHGVEYFLHAVKYEAQGERAKESIRAKCLQ 339
SN ++AI+ V +A ED NY +A LY + +EYF +KYE + + +E+I K +
Sbjct: 3 SNFKEQAIEYVKQAVHEDNAGNYNKAFPLYMNALEYFKTHLKYE-KNPKIREAITQKFTE 61
Query: 340 YLDRA------XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXLQGKLEGA 501
YL RA L+ L A
Sbjct: 62 YLRRAEEIRAVLDEGGSGPGSNGDAAVATRPKTKPKDGEGGGKDGEDPEQSKLRAGLNSA 121
Query: 502 IVVEKPHVKWSDVXGXXXXXXXXXXXVILPIKFSTFVYWQENTMEGHLIILDPLVLVSPY 681
IV EKP++KWSDV G VILP+KF F + L+ P S Y
Sbjct: 122 IVREKPNIKWSDVAGLESAKQALQEAVILPVKFPQFFTGKRRPWRAFLLYGPPGTGKS-Y 180
Query: 682 LAKA 693
LAKA
Sbjct: 181 LAKA 184
>At1g08270.2 68414.m00912 expressed protein low similarity to
SP|P46467 SKD1 protein (Vacuolar sorting protein 4b)
{Mus musculus}
Length = 132
Score = 37.1 bits (82), Expect = 0.012
Identities = 31/119 (26%), Positives = 44/119 (36%)
Frame = +1
Query: 253 HGVEYFLHAVKYEAQGERAKESIRAKCLQYLDRAXXXXXXXXXXXXXXXXXXXXXXXXXX 432
+ +EYF +KYE + R +++I K +YL RA
Sbjct: 2 NALEYFKIYLKYE-KNPRIRDAITDKFYEYLRRAEEIRAVLVEVGSGKGSNGDAAVATKP 60
Query: 433 XXXXXXXXXXXXXXXLQGKLEGAIVVEKPHVKWSDVXGXXXXXXXXXXXVILPIKFSTF 609
L+ L AI+ EKP+VKW+ V G VILP+ F F
Sbjct: 61 KTKPKDGGDGEESK-LRAGLNSAIIREKPNVKWTVVAGLESAKQALKEAVILPVNFPQF 118
>At1g08270.1 68414.m00913 expressed protein low similarity to
SP|P46467 SKD1 protein (Vacuolar sorting protein 4b)
{Mus musculus}
Length = 126
Score = 37.1 bits (82), Expect = 0.012
Identities = 31/119 (26%), Positives = 44/119 (36%)
Frame = +1
Query: 253 HGVEYFLHAVKYEAQGERAKESIRAKCLQYLDRAXXXXXXXXXXXXXXXXXXXXXXXXXX 432
+ +EYF +KYE + R +++I K +YL RA
Sbjct: 2 NALEYFKIYLKYE-KNPRIRDAITDKFYEYLRRAEEIRAVLVEVGSGKGSNGDAAVATKP 60
Query: 433 XXXXXXXXXXXXXXXLQGKLEGAIVVEKPHVKWSDVXGXXXXXXXXXXXVILPIKFSTF 609
L+ L AI+ EKP+VKW+ V G VILP+ F F
Sbjct: 61 KTKPKDGGDGEESK-LRAGLNSAIIREKPNVKWTVVAGLESAKQALKEAVILPVNFPQF 118
>At3g27120.1 68416.m03393 spastin ATPase, putative similar to
SWISS-PROT:Q9QYY8 spastin (Fragment) [Mus musculus];
contains Pfam domain, PF00004: ATPase, AAA family
Length = 287
Score = 31.1 bits (67), Expect = 0.82
Identities = 11/19 (57%), Positives = 15/19 (78%)
Frame = +3
Query: 633 GRASYYFGPPGTGKSILGK 689
G+ FGPPGTGK+++GK
Sbjct: 43 GKGLLLFGPPGTGKTMIGK 61
>At2g34560.2 68415.m04246 katanin, putative similar to katanin p60
subunit [Strongylocentrotus purpuratus] GI:3098603;
contains Pfam profile PF00004: ATPase AAA family
Length = 393
Score = 31.1 bits (67), Expect = 0.82
Identities = 19/44 (43%), Positives = 24/44 (54%)
Frame = +2
Query: 599 FPHLFTGKRIPWKGILLFWTPWYW*VHTWQRLGATEANNSTFFS 730
+P F G PWKGILLF P + + ATE N+TFF+
Sbjct: 134 YPTYFNGLLTPWKGILLFGPPGTGKTMLAKAV-ATEC-NTTFFN 175
Score = 29.5 bits (63), Expect = 2.5
Identities = 18/72 (25%), Positives = 30/72 (41%)
Frame = +1
Query: 478 LQGKLEGAIVVEKPHVKWSDVXGXXXXXXXXXXXVILPIKFSTFVYWQENTMEGHLIILD 657
L L I+ P++KW + G V++PIK+ T+ +G +++
Sbjct: 94 LAESLSRDIIRGNPNIKWESIKGLENAKKLLKEAVVMPIKYPTYFNGLLTPWKG-ILLFG 152
Query: 658 PLVLVSPYLAKA 693
P LAKA
Sbjct: 153 PPGTGKTMLAKA 164
>At2g34560.1 68415.m04245 katanin, putative similar to katanin p60
subunit [Strongylocentrotus purpuratus] GI:3098603;
contains Pfam profile PF00004: ATPase AAA family
Length = 384
Score = 31.1 bits (67), Expect = 0.82
Identities = 19/44 (43%), Positives = 24/44 (54%)
Frame = +2
Query: 599 FPHLFTGKRIPWKGILLFWTPWYW*VHTWQRLGATEANNSTFFS 730
+P F G PWKGILLF P + + ATE N+TFF+
Sbjct: 125 YPTYFNGLLTPWKGILLFGPPGTGKTMLAKAV-ATEC-NTTFFN 166
Score = 29.5 bits (63), Expect = 2.5
Identities = 18/72 (25%), Positives = 30/72 (41%)
Frame = +1
Query: 478 LQGKLEGAIVVEKPHVKWSDVXGXXXXXXXXXXXVILPIKFSTFVYWQENTMEGHLIILD 657
L L I+ P++KW + G V++PIK+ T+ +G +++
Sbjct: 85 LAESLSRDIIRGNPNIKWESIKGLENAKKLLKEAVVMPIKYPTYFNGLLTPWKG-ILLFG 143
Query: 658 PLVLVSPYLAKA 693
P LAKA
Sbjct: 144 PPGTGKTMLAKA 155
>At1g80350.1 68414.m09406 katanin 1 (KTN1) identical to katanin 1
(KTN1) [Arabidopsis thaliana] GI:14133602
Length = 523
Score = 29.9 bits (64), Expect = 1.9
Identities = 11/20 (55%), Positives = 13/20 (65%)
Frame = +2
Query: 602 PHLFTGKRIPWKGILLFWTP 661
P F G R PWKG+L+F P
Sbjct: 262 PEYFQGIRRPWKGVLMFGPP 281
Score = 28.3 bits (60), Expect = 5.8
Identities = 10/13 (76%), Positives = 12/13 (92%)
Frame = +3
Query: 651 FGPPGTGKSILGK 689
FGPPGTGK++L K
Sbjct: 278 FGPPGTGKTLLAK 290
>At4g04910.1 68417.m00714 AAA-type ATPase family protein similar to
SP|P18708 Vesicular-fusion protein NSF
(N-ethylmaleimide-sensitive fusion protein)
(NEM-sensitive fusion protein) {Cricetulus griseus};
contains Pfam profiles PF00004: ATPase AAA family,
PF02359: Cell division protein 48 (CDC48) N-terminal
domain; contains non-consensus AT-AC splice sites at
intron 2
Length = 742
Score = 29.5 bits (63), Expect = 2.5
Identities = 13/40 (32%), Positives = 22/40 (55%), Gaps = 2/40 (5%)
Frame = +3
Query: 585 FTNKIF--HICLLAREYHGRASYYFGPPGTGKSILGKGWG 698
F +++F H+ H + FGPPGTGK+++ + G
Sbjct: 232 FASRVFPPHVTSRLGIKHVKGMLLFGPPGTGKTLMARQIG 271
>At2g26910.1 68415.m03228 ABC transporter family protein similar to
PDR5-like ABC transporter GI:1514643 from [Spirodela
polyrhiza]
Length = 1420
Score = 29.1 bits (62), Expect = 3.3
Identities = 11/22 (50%), Positives = 16/22 (72%)
Frame = +2
Query: 605 HLFTGKRIPWKGILLFWTPWYW 670
+LF+G IP+K I L+W +YW
Sbjct: 1320 NLFSGFMIPYKRIPLWWRWYYW 1341
>At5g61510.1 68418.m07718 NADP-dependent oxidoreductase, putative
similar to zeta-crystallin homolog TED2 from Zinnia
elegans [gi:531096]; contains Pfam zinc-binding
dehydrogenase domain PF00107
Length = 406
Score = 28.7 bits (61), Expect = 4.4
Identities = 19/61 (31%), Positives = 28/61 (45%)
Frame = +1
Query: 148 TMTSSNTLQKAIDLVTKATEEDKNKNYEEALRLYEHGVEYFLHAVKYEAQGERAKESIRA 327
++++ T+ L T A EE K + +R+YEHG L E GE + IR
Sbjct: 57 SLSTRGTVFAVRALSTVAVEESSEKKMVKGIRVYEHGGPEVLKWEDVEV-GEPKEGEIRV 115
Query: 328 K 330
K
Sbjct: 116 K 116
>At3g19740.1 68416.m02499 AAA-type ATPase family protein contains
Pfam domain, PF00004: ATPase, AAA family
Length = 439
Score = 28.3 bits (60), Expect = 5.8
Identities = 10/13 (76%), Positives = 12/13 (92%)
Frame = +3
Query: 651 FGPPGTGKSILGK 689
FGPPGTGK++L K
Sbjct: 191 FGPPGTGKTLLAK 203
>At1g64110.2 68414.m07264 AAA-type ATPase family protein contains
Pfam domain, PF00004: ATPase, AAA family
Length = 829
Score = 28.3 bits (60), Expect = 5.8
Identities = 11/18 (61%), Positives = 13/18 (72%)
Frame = +3
Query: 636 RASYYFGPPGTGKSILGK 689
R FGPPGTGK++L K
Sbjct: 556 RGILLFGPPGTGKTMLAK 573
>At1g64110.1 68414.m07263 AAA-type ATPase family protein contains
Pfam domain, PF00004: ATPase, AAA family
Length = 824
Score = 28.3 bits (60), Expect = 5.8
Identities = 11/18 (61%), Positives = 13/18 (72%)
Frame = +3
Query: 636 RASYYFGPPGTGKSILGK 689
R FGPPGTGK++L K
Sbjct: 551 RGILLFGPPGTGKTMLAK 568
>At1g50140.1 68414.m05623 AAA-type ATPase family protein contains
Pfam domain, PF00004: ATPase, AAA family
Length = 640
Score = 28.3 bits (60), Expect = 5.8
Identities = 10/13 (76%), Positives = 12/13 (92%)
Frame = +3
Query: 651 FGPPGTGKSILGK 689
FGPPGTGK++L K
Sbjct: 392 FGPPGTGKTLLAK 404
>At4g24860.1 68417.m03559 AAA-type ATPase family protein contains
Pfam profile PF00004: ATPase, AAA family
Length = 1122
Score = 27.9 bits (59), Expect = 7.6
Identities = 10/13 (76%), Positives = 12/13 (92%)
Frame = +3
Query: 651 FGPPGTGKSILGK 689
FGPPGTGK++L K
Sbjct: 861 FGPPGTGKTMLAK 873
>At4g02480.1 68417.m00335 AAA-type ATPase family protein contains Pfam
domain, PF00004: ATPase, AAA family; similar to Spastin
(Swiss-Prot:Q9UBP0) [Homo sapiens] and Spastin (Fragment)
(Swiss-Prot:Q9QYY8) [Mus musculus]; similar to
mitochondrial sorting protein 1 (MSP1) protein
(TAT-binding homolog 4) (Swiss-Prot:P28737)
[Saccharomyces cerevisiae]
Length = 1265
Score = 27.9 bits (59), Expect = 7.6
Identities = 10/13 (76%), Positives = 12/13 (92%)
Frame = +3
Query: 651 FGPPGTGKSILGK 689
FGPPGTGK++L K
Sbjct: 1004 FGPPGTGKTMLAK 1016
>At3g60160.1 68416.m06717 ABC transporter family protein similar to
ATP-binding cassette transporter MRP8 GI:18031899 from
[Arabidopsis thaliana]
Length = 1490
Score = 27.9 bits (59), Expect = 7.6
Identities = 22/61 (36%), Positives = 29/61 (47%), Gaps = 8/61 (13%)
Frame = -3
Query: 421 FWIPRPLLVFSFDLF*DI----PLIFQLYQDIEGILHEYFLLPFHL----GPHILLHEEN 266
+W+ +L FSFD PL FQ Y D+ G+L FLL + G H+L N
Sbjct: 154 WWLCSFILSFSFDAHFITAKHEPLEFQDYADLTGLLASLFLLAVSIRGKTGFHLLESSGN 213
Query: 265 T 263
T
Sbjct: 214 T 214
>At1g02890.1 68414.m00256 AAA-type ATPase family protein contains Pfam
domain, PF00004: ATPase, AAA family; similar to
mitochondrial sorting protein 1 (MSP1) (TAT-binding
homolog 4) (Swiss-Prot:P28737) [Saccharomyces cerevisiae]
Length = 1252
Score = 27.9 bits (59), Expect = 7.6
Identities = 10/13 (76%), Positives = 12/13 (92%)
Frame = +3
Query: 651 FGPPGTGKSILGK 689
FGPPGTGK++L K
Sbjct: 991 FGPPGTGKTMLAK 1003
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 13,298,748
Number of Sequences: 28952
Number of extensions: 235768
Number of successful extensions: 748
Number of sequences better than 10.0: 18
Number of HSP's better than 10.0 without gapping: 708
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 744
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1663169840
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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