Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= brP-0035
(750 letters)
Database: bee
438 sequences; 146,343 total letters
Searching......................................................done
Score E
Sequences producing significant alignments: (bits) Value
AF015267-1|AAC38959.1| 461|Apis mellifera elongation factor-1al... 392 e-111
X52884-1|CAA37066.1| 461|Apis mellifera elongation factor 1 alp... 382 e-108
AY208278-1|AAO48970.1| 274|Apis mellifera elongation factor 1-a... 273 1e-75
EF013389-1|ABK54743.1| 172|Apis mellifera elongation factor 1-a... 240 9e-66
AF069739-1|AAC63272.2| 690|Apis mellifera translation initiatio... 33 0.004
AM050259-1|CAJ18340.1| 683|Apis mellifera putative H3K9 methylt... 24 1.3
DQ244074-1|ABB36784.1| 517|Apis mellifera cytochrome P450 monoo... 23 3.1
AB253417-1|BAE86928.1| 567|Apis mellifera alpha-glucosidase pro... 23 3.1
D79208-1|BAA11466.1| 567|Apis mellifera alpha-glucosidase protein. 23 4.0
AB253415-1|BAE86926.1| 588|Apis mellifera alpha-glucosidase pro... 21 9.3
>AF015267-1|AAC38959.1| 461|Apis mellifera elongation factor-1alpha
F2 protein.
Length = 461
Score = 392 bits (964), Expect = e-111
Identities = 187/195 (95%), Positives = 187/195 (95%)
Frame = +2
Query: 38 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 217
MGKEK HINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL
Sbjct: 1 MGKEKIHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 60
Query: 218 DKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVXAGT 397
DKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIV AGT
Sbjct: 61 DKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGT 120
Query: 398 GEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDXTEPPYSEPRFEEIKKEVCSYXKK 577
GEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMD TEPPYSE RFEEIKKEV SY KK
Sbjct: 121 GEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSETRFEEIKKEVSSYIKK 180
Query: 578 IGYNPAAXAFVPIXG 622
IGYNPAA AFVPI G
Sbjct: 181 IGYNPAAVAFVPISG 195
Score = 58.8 bits (136), Expect = 5e-11
Identities = 23/32 (71%), Positives = 27/32 (84%)
Frame = +1
Query: 631 GDNMLEPSTKMPWFRGWQVERKEGKADGNALL 726
GDNMLE S+KMPWF+GW VERKEGK +G L+
Sbjct: 198 GDNMLEVSSKMPWFKGWTVERKEGKVEGKCLI 229
Score = 29.1 bits (62), Expect = 0.047
Identities = 16/46 (34%), Positives = 21/46 (45%)
Frame = +3
Query: 612 PXVGWHGRQHVGAFNQNALVQGMAGGA*GRQSXRKCLIEXLDAXLP 749
P GWHG + ++ +G + KCLIE LDA LP
Sbjct: 192 PISGWHGDNMLEVSSKMPWFKGWTVERKEGKVEGKCLIEALDAILP 237
>X52884-1|CAA37066.1| 461|Apis mellifera elongation factor 1 alpha
protein.
Length = 461
Score = 382 bits (941), Expect = e-108
Identities = 182/195 (93%), Positives = 185/195 (94%)
Frame = +2
Query: 38 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 217
MGKEK HINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL
Sbjct: 1 MGKEKIHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 60
Query: 218 DKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVXAGT 397
DKLKAERERGITIDIALWKFET+KYYVTIIDAPGHRDFIKNMITGTSQADCAVLIV AG
Sbjct: 61 DKLKAERERGITIDIALWKFETAKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGI 120
Query: 398 GEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDXTEPPYSEPRFEEIKKEVCSYXKK 577
GEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMD T+PPYSE RFEEIKKEV SY KK
Sbjct: 121 GEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDMTDPPYSEARFEEIKKEVSSYIKK 180
Query: 578 IGYNPAAXAFVPIXG 622
IGYN A+ AFVPI G
Sbjct: 181 IGYNTASVAFVPISG 195
Score = 58.8 bits (136), Expect = 5e-11
Identities = 22/32 (68%), Positives = 27/32 (84%)
Frame = +1
Query: 631 GDNMLEPSTKMPWFRGWQVERKEGKADGNALL 726
GDNMLEPS K PW++GW+VERK+G ADG L+
Sbjct: 198 GDNMLEPSPKTPWYKGWKVERKDGNADGKTLI 229
>AY208278-1|AAO48970.1| 274|Apis mellifera elongation factor
1-alpha protein.
Length = 274
Score = 273 bits (670), Expect = 1e-75
Identities = 131/138 (94%), Positives = 131/138 (94%)
Frame = +2
Query: 209 WVLDKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVX 388
WVLDKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIV
Sbjct: 1 WVLDKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVA 60
Query: 389 AGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDXTEPPYSEPRFEEIKKEVCSY 568
AGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMD TEPPYSE RFEEIKKEV SY
Sbjct: 61 AGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSETRFEEIKKEVSSY 120
Query: 569 XKKIGYNPAAXAFVPIXG 622
KKIGYNPAA AFVPI G
Sbjct: 121 IKKIGYNPAAVAFVPISG 138
Score = 58.8 bits (136), Expect = 5e-11
Identities = 23/32 (71%), Positives = 27/32 (84%)
Frame = +1
Query: 631 GDNMLEPSTKMPWFRGWQVERKEGKADGNALL 726
GDNMLE S+KMPWF+GW VERKEGK +G L+
Sbjct: 141 GDNMLEVSSKMPWFKGWTVERKEGKVEGKCLI 172
Score = 29.1 bits (62), Expect = 0.047
Identities = 16/46 (34%), Positives = 21/46 (45%)
Frame = +3
Query: 612 PXVGWHGRQHVGAFNQNALVQGMAGGA*GRQSXRKCLIEXLDAXLP 749
P GWHG + ++ +G + KCLIE LDA LP
Sbjct: 135 PISGWHGDNMLEVSSKMPWFKGWTVERKEGKVEGKCLIEALDAILP 180
>EF013389-1|ABK54743.1| 172|Apis mellifera elongation factor
1-alpha protein.
Length = 172
Score = 240 bits (588), Expect = 9e-66
Identities = 115/122 (94%), Positives = 115/122 (94%)
Frame = +2
Query: 257 DIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVXAGTGEFEAGISKNGQT 436
DIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIV AGTGEFEAGISKNGQT
Sbjct: 1 DIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQT 60
Query: 437 REHALLAFTLGVKQLIVGVNKMDXTEPPYSEPRFEEIKKEVCSYXKKIGYNPAAXAFVPI 616
REHALLAFTLGVKQLIVGVNKMD TEPPYSE RFEEIKKEV SY KKIGYNPAA AFVPI
Sbjct: 61 REHALLAFTLGVKQLIVGVNKMDSTEPPYSETRFEEIKKEVSSYIKKIGYNPAAVAFVPI 120
Query: 617 XG 622
G
Sbjct: 121 SG 122
Score = 58.8 bits (136), Expect = 5e-11
Identities = 23/32 (71%), Positives = 27/32 (84%)
Frame = +1
Query: 631 GDNMLEPSTKMPWFRGWQVERKEGKADGNALL 726
GDNMLE S+KMPWF+GW VERKEGK +G L+
Sbjct: 125 GDNMLEVSSKMPWFKGWTVERKEGKVEGKCLI 156
Score = 29.1 bits (62), Expect = 0.047
Identities = 16/46 (34%), Positives = 21/46 (45%)
Frame = +3
Query: 612 PXVGWHGRQHVGAFNQNALVQGMAGGA*GRQSXRKCLIEXLDAXLP 749
P GWHG + ++ +G + KCLIE LDA LP
Sbjct: 119 PISGWHGDNMLEVSSKMPWFKGWTVERKEGKVEGKCLIEALDAILP 164
>AF069739-1|AAC63272.2| 690|Apis mellifera translation initiation
factor 2 protein.
Length = 690
Score = 32.7 bits (71), Expect = 0.004
Identities = 25/70 (35%), Positives = 32/70 (45%)
Frame = +2
Query: 296 VTIIDAPGHRDFIKNMITGTSQADCAVLIVXAGTGEFEAGISKNGQTREHALLAFTLGVK 475
VT +D PGH FI G D VL+V A G E QT + +A V
Sbjct: 195 VTFLDTPGHAAFISMRHRGAHITDIVVLVVAADDGVKE-------QTLQSIEMAKDAKV- 246
Query: 476 QLIVGVNKMD 505
+IV +NK+D
Sbjct: 247 PIIVAINKID 256
Score = 25.4 bits (53), Expect = 0.57
Identities = 9/18 (50%), Positives = 13/18 (72%)
Frame = +2
Query: 50 KTHINIVVIGHVDSGKST 103
K H + ++GHVD GK+T
Sbjct: 143 KRHPIVTIMGHVDHGKTT 160
>AM050259-1|CAJ18340.1| 683|Apis mellifera putative H3K9
methyltransferase protein.
Length = 683
Score = 24.2 bits (50), Expect = 1.3
Identities = 11/15 (73%), Positives = 11/15 (73%)
Frame = +2
Query: 59 INIVVIGHVDSGKST 103
INI IGHV GKST
Sbjct: 43 INIGTIGHVAHGKST 57
>DQ244074-1|ABB36784.1| 517|Apis mellifera cytochrome P450
monooxygenase protein.
Length = 517
Score = 23.0 bits (47), Expect = 3.1
Identities = 10/17 (58%), Positives = 12/17 (70%)
Frame = -1
Query: 51 FSLPIFG*SRITNCV*Y 1
FSLPIFG I +C+ Y
Sbjct: 57 FSLPIFGTRWIFSCIGY 73
>AB253417-1|BAE86928.1| 567|Apis mellifera alpha-glucosidase
protein.
Length = 567
Score = 23.0 bits (47), Expect = 3.1
Identities = 13/34 (38%), Positives = 18/34 (52%)
Frame = +2
Query: 281 TSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLI 382
T KYY D P + FIKN+ ++ +D LI
Sbjct: 294 TMKYYDYGADFPFNFAFIKNVSRDSNSSDFKKLI 327
>D79208-1|BAA11466.1| 567|Apis mellifera alpha-glucosidase protein.
Length = 567
Score = 22.6 bits (46), Expect = 4.0
Identities = 12/34 (35%), Positives = 18/34 (52%)
Frame = +2
Query: 281 TSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLI 382
T KYY D P + FIKN+ ++ +D L+
Sbjct: 294 TMKYYDYGADFPFNFAFIKNVSRDSNSSDFKKLV 327
>AB253415-1|BAE86926.1| 588|Apis mellifera alpha-glucosidase
protein.
Length = 588
Score = 21.4 bits (43), Expect = 9.3
Identities = 8/18 (44%), Positives = 12/18 (66%)
Frame = -3
Query: 553 LLDFLKSGLTVWWFSXIH 500
L F++SG+T W S I+
Sbjct: 56 LSHFIESGITAIWLSPIN 73
Database: bee
Posted date: Oct 23, 2007 1:17 PM
Number of letters in database: 146,343
Number of sequences in database: 438
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 203,604
Number of Sequences: 438
Number of extensions: 3986
Number of successful extensions: 25
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 16
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 25
length of database: 146,343
effective HSP length: 56
effective length of database: 121,815
effective search space used: 23510295
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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