Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= brP-0017
(800 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 200 2e-52
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 196 2e-51
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 101 2e-22
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 79 1e-15
SPAPB18E9.04c |||sequence orphan|Schizosaccharomyces pombe|chr 1... 32 0.083
SPAPJ760.02c |app1||App1 protein|Schizosaccharomyces pombe|chr 1... 30 0.44
SPAC3C7.09 |set8||lysine methyltransferase Set8 |Schizosaccharom... 26 5.4
SPCC18.03 |||shuttle craft like transcriptional regulator|Schizo... 26 7.2
SPAC30D11.13 |hus5|ubc9|SUMO conjugating enzyme Hus5|Schizosacch... 26 7.2
SPAC222.10c |byr4||two-component GAP Byr4|Schizosaccharomyces po... 26 7.2
SPAC1B3.15c |||membrane transporter|Schizosaccharomyces pombe|ch... 26 7.2
>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 455
Score = 200 bits (488), Expect = 2e-52
Identities = 90/117 (76%), Positives = 104/117 (88%)
Frame = +1
Query: 7 SLLMERLSVDYGKKSKLEFAIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDXEAXY 186
+LL+ERL+++YGKKS L+F++YPAPQVST+VVEPYNS+LTTH TL++SDC FMVD EA Y
Sbjct: 155 ALLLERLNMEYGKKSNLQFSVYPAPQVSTSVVEPYNSVLTTHATLDNSDCTFMVDNEACY 214
Query: 187 DICRRNLDIERPTYTNLNRLIGQIVSSITXSLRXDGAXNVDLTEFQTNLVPYPRXHF 357
DICRRNLDIERPTY NLNRLI Q+VSSIT SLR G+ NVDL EFQTNLVPYPR HF
Sbjct: 215 DICRRNLDIERPTYENLNRLIAQVVSSITASLRFAGSLNVDLNEFQTNLVPYPRIHF 271
Score = 188 bits (459), Expect = 6e-49
Identities = 82/121 (67%), Positives = 97/121 (80%)
Frame = +2
Query: 344 PVXTLPLVTYAPVISAXKAYHEQLSVAXITNACFEPANXMVKCDPRHGKYMACCMLYRGD 523
P PLVTY+P++SA KA+HE SV ITN CFEP N MVKCDPR G+YMA C+LYRGD
Sbjct: 267 PRIHFPLVTYSPIVSAAKAFHESNSVQEITNQCFEPYNQMVKCDPRTGRYMATCLLYRGD 326
Query: 524 VVPXDVNAAIXTIKTKRTIQFVDWCPTGFKVGINYQPPTVVPGGDLAKVQRXVCMLSNTT 703
V+P DV AA+ +IK++RTIQFVDWCPTGFK+GI Y+PP VPG +AKV R VCMLSNTT
Sbjct: 327 VIPRDVQAAVTSIKSRRTIQFVDWCPTGFKIGICYEPPQHVPGSGIAKVNRAVCMLSNTT 386
Query: 704 A 706
+
Sbjct: 387 S 387
Score = 50.4 bits (115), Expect = 3e-07
Identities = 20/25 (80%), Positives = 22/25 (88%)
Frame = +1
Query: 724 ARLDHKFDLMYXKRAFVHWYLRXGM 798
+RLDHKFDLMY KRAFVHWY+ GM
Sbjct: 393 SRLDHKFDLMYSKRAFVHWYVGEGM 417
>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 449
Score = 196 bits (479), Expect = 2e-51
Identities = 88/119 (73%), Positives = 105/119 (88%)
Frame = +1
Query: 1 FTSLLMERLSVDYGKKSKLEFAIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDXEA 180
F +LL+ERL+++Y KKSKL+F++YPAPQVST+VVEPYNS+LTTH TL+ +DC FMVD E+
Sbjct: 149 FGALLLERLAMEYTKKSKLQFSVYPAPQVSTSVVEPYNSVLTTHATLDLADCTFMVDNES 208
Query: 181 XYDICRRNLDIERPTYTNLNRLIGQIVSSITXSLRXDGAXNVDLTEFQTNLVPYPRXHF 357
YDICRRNLDIERP+Y NLNRLI Q+VSSIT SLR +G+ NVDL EFQTNLVPYPR HF
Sbjct: 209 CYDICRRNLDIERPSYENLNRLIAQVVSSITASLRFEGSLNVDLAEFQTNLVPYPRIHF 267
Score = 185 bits (451), Expect = 6e-48
Identities = 82/121 (67%), Positives = 95/121 (78%)
Frame = +2
Query: 344 PVXTLPLVTYAPVISAXKAYHEQLSVAXITNACFEPANXMVKCDPRHGKYMACCMLYRGD 523
P PLVTYAP++SA KA+HE SV ITN CFEP N MVKCDPR G+YMA C+LYRGD
Sbjct: 263 PRIHFPLVTYAPIVSAAKAFHESNSVQEITNQCFEPYNQMVKCDPRAGRYMATCLLYRGD 322
Query: 524 VVPXDVNAAIXTIKTKRTIQFVDWCPTGFKVGINYQPPTVVPGGDLAKVQRXVCMLSNTT 703
V+P DV AA+ TIK KRTIQFVDWCPTGFK+GI +PP + G ++AKV R VCMLSNTT
Sbjct: 323 VIPRDVQAAVTTIKAKRTIQFVDWCPTGFKIGICDRPPQHIEGSEIAKVDRAVCMLSNTT 382
Query: 704 A 706
+
Sbjct: 383 S 383
Score = 50.4 bits (115), Expect = 3e-07
Identities = 20/25 (80%), Positives = 22/25 (88%)
Frame = +1
Query: 724 ARLDHKFDLMYXKRAFVHWYLRXGM 798
+RLDHKFDLMY KRAFVHWY+ GM
Sbjct: 389 SRLDHKFDLMYSKRAFVHWYVGEGM 413
>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 448
Score = 101 bits (241), Expect = 2e-22
Identities = 45/117 (38%), Positives = 70/117 (59%)
Frame = +1
Query: 7 SLLMERLSVDYGKKSKLEFAIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDXEAXY 186
+LL+ ++ +Y + F++ PAP+ S VVEPYN+ L+ H +E+SD F +D EA
Sbjct: 149 TLLLSKIREEYPDRMMATFSVAPAPKSSDTVVEPYNATLSMHQLVENSDETFCIDNEALS 208
Query: 187 DICRRNLDIERPTYTNLNRLIGQIVSSITXSLRXDGAXNVDLTEFQTNLVPYPRXHF 357
I L I+ P+Y +LN L+ +++ +T S R G N DL + N+VP+PR HF
Sbjct: 209 SIFANTLKIKSPSYDDLNHLVSAVMAGVTTSFRFPGELNSDLRKLAVNMVPFPRLHF 265
Score = 67.7 bits (158), Expect = 2e-12
Identities = 30/92 (32%), Positives = 50/92 (54%)
Frame = +2
Query: 362 LVTYAPVISAXKAYHEQLSVAXITNACFEPANXMVKCDPRHGKYMACCMLYRGDVVPXDV 541
+V +AP+ + + + +SV +T F+ N MV DPRHG+Y+ L+RG V +V
Sbjct: 267 MVGFAPLAAIGSSSFQAVSVPELTQQMFDANNMMVAADPRHGRYLTVAALFRGKVSMKEV 326
Query: 542 NAAIXTIKTKRTIQFVDWCPTGFKVGINYQPP 637
+ I +++TK + FV+W P + PP
Sbjct: 327 DEQIRSVQTKNSAYFVEWIPDNVLKAVCSVPP 358
Score = 31.5 bits (68), Expect = 0.14
Identities = 11/24 (45%), Positives = 16/24 (66%)
Frame = +1
Query: 727 RLDHKFDLMYXKRAFVHWYLRXGM 798
RL +F M+ ++AF+HWY GM
Sbjct: 380 RLGDQFSAMFRRKAFLHWYTGEGM 403
>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 446
Score = 78.6 bits (185), Expect = 1e-15
Identities = 42/118 (35%), Positives = 64/118 (54%), Gaps = 1/118 (0%)
Frame = +1
Query: 7 SLLMERLSVDYGKKSKLEFAIYPAPQ-VSTAVVEPYNSILTTHTTLEHSDCAFMVDXEAX 183
S L+ERL+ Y KK ++++P Q VS VV+PYNS+L ++D ++D A
Sbjct: 151 SFLLERLNDRYPKKIIQTYSVFPNSQSVSDVVVQPYNSLLALKRLTLNADSVVVLDNAAL 210
Query: 184 YDICRRNLDIERPTYTNLNRLIGQIVSSITXSLRXDGAXNVDLTEFQTNLVPYPRXHF 357
I L + PT+ N+L+ ++S+ T +LR G N DL +L+P PR HF
Sbjct: 211 AHIAADRLHTQNPTFHQQNQLVSTVMSASTTTLRYPGYMNNDLVSIIASLIPSPRCHF 268
Score = 37.9 bits (84), Expect = 0.002
Identities = 16/66 (24%), Positives = 35/66 (53%), Gaps = 1/66 (1%)
Frame = +2
Query: 449 PANXMVKCDP-RHGKYMACCMLYRGDVVPXDVNAAIXTIKTKRTIQFVDWCPTGFKVGIN 625
P N MV +P + +++ + +G+ P DV+ ++ I+ +R F+ W P +V ++
Sbjct: 302 PKNQMVSVNPSKKSCFISILDIIQGEADPADVHKSLLRIRERRYASFIPWGPASIQVALS 361
Query: 626 YQPPTV 643
+ P +
Sbjct: 362 KKSPYI 367
>SPAPB18E9.04c |||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 800
Score = 32.3 bits (70), Expect = 0.083
Identities = 25/91 (27%), Positives = 38/91 (41%), Gaps = 2/91 (2%)
Frame = +3
Query: 372 TRQSSLPXRPTM--NSFPSPXSQTHASSPPTXW*NATPVMASTWLAVCCTVVTSYPXM*T 545
T S+ P PT+ S +P + T+ ++ + +TPV ++ CT TS P T
Sbjct: 402 TGNSTTPVTPTVPPTSSSTPLTTTNCTTSTSVPYTSTPVTSTPLATTNCTTSTSVPY--T 459
Query: 546 RPSXPSKPSVLSNSSTGVQPVSRSVSTTSHP 638
S P +N +T S TS P
Sbjct: 460 STPVTSTPLTTTNCTTSTSIPYTSTPVTSTP 490
Score = 28.7 bits (61), Expect = 1.0
Identities = 19/71 (26%), Positives = 34/71 (47%)
Frame = +3
Query: 420 SPXSQTHASSPPTXW*NATPVMASTWLAVCCTVVTSYPXM*TRPSXPSKPSVLSNSSTGV 599
+P + T+ ++ + +TPV ++ CT TS P T P S ++ S++
Sbjct: 525 TPVTTTNCTTSTSVLYTSTPVTSTPLATTNCTTSTSVPYTST-PVTSSNYTISSSTPVTS 583
Query: 600 QPVSRSVSTTS 632
PV+ + TTS
Sbjct: 584 TPVTTTNCTTS 594
Score = 28.3 bits (60), Expect = 1.3
Identities = 21/70 (30%), Positives = 34/70 (48%)
Frame = +3
Query: 420 SPXSQTHASSPPTXW*NATPVMASTWLAVCCTVVTSYPXM*TRPSXPSKPSVLSNSSTGV 599
+P + T+ ++ + +TPV +S + T VTS P T + + SVL S+
Sbjct: 548 TPLATTNCTTSTSVPYTSTPVTSSNYTISSSTPVTSTPV--TTTNCTTSTSVLYTSTPIT 605
Query: 600 QPVSRSVSTT 629
P S S S+T
Sbjct: 606 SPNSTSSSST 615
Score = 26.6 bits (56), Expect = 4.1
Identities = 22/87 (25%), Positives = 36/87 (41%)
Frame = +3
Query: 372 TRQSSLPXRPTMNSFPSPXSQTHASSPPTXW*NATPVMASTWLAVCCTVVTSYPXM*TRP 551
T +S+P T + +P + T+ ++ + +TPV ++ CT TS P T
Sbjct: 428 TTSTSVPYTSTPVT-STPLATTNCTTSTSVPYTSTPVTSTPLTTTNCTTSTSIPY--TST 484
Query: 552 SXPSKPSVLSNSSTGVQPVSRSVSTTS 632
S P +N +T S TS
Sbjct: 485 PVTSTPLTTTNCTTSTSVPYTSTPVTS 511
Score = 25.8 bits (54), Expect = 7.2
Identities = 24/88 (27%), Positives = 42/88 (47%), Gaps = 1/88 (1%)
Frame = +3
Query: 372 TRQSSLPXRPTMNSFPSPXSQTHASSPPTXW*NATPVMASTWLAVCCTVVTSYPXM*TRP 551
T +S+P T + +P + T+ ++ + +TPV +S + T VTS P T
Sbjct: 474 TTSTSIPYTSTPVT-STPLTTTNCTTSTSVPYTSTPVTSSNYTISSSTPVTSTPV--TTT 530
Query: 552 SXPSKPSVLSNSSTGVQ-PVSRSVSTTS 632
+ + SVL S+ P++ + TTS
Sbjct: 531 NCTTSTSVLYTSTPVTSTPLATTNCTTS 558
>SPAPJ760.02c |app1||App1 protein|Schizosaccharomyces pombe|chr
1|||Manual
Length = 857
Score = 29.9 bits (64), Expect = 0.44
Identities = 16/45 (35%), Positives = 20/45 (44%)
Frame = +3
Query: 516 VVTSYPXM*TRPSXPSKPSVLSNSSTGVQPVSRSVSTTSHPPWCP 650
VV P + RP+ P P LS V PV+ V + PP P
Sbjct: 552 VVPEAPSVPQRPAVPVVPEALSVPQPPVAPVAPEVPSVPQPPVAP 596
>SPAC3C7.09 |set8||lysine methyltransferase Set8
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 429
Score = 26.2 bits (55), Expect = 5.4
Identities = 7/17 (41%), Positives = 13/17 (76%)
Frame = -1
Query: 683 RRHVEPWPSRLRAPRWV 633
+R+ + WPS++ P+WV
Sbjct: 153 QRYSDSWPSKITLPKWV 169
>SPCC18.03 |||shuttle craft like transcriptional
regulator|Schizosaccharomyces pombe|chr 3|||Manual
Length = 1077
Score = 25.8 bits (54), Expect = 7.2
Identities = 13/42 (30%), Positives = 17/42 (40%)
Frame = +2
Query: 428 ITNACFEPANXMVKCDPRHGKYMACCMLYRGDVVPXDVNAAI 553
+ ACFEP N C H K C L + D N ++
Sbjct: 361 LCGACFEPINAKCYCG-LHSKTYPCSSLPSPSISKKDENGSV 401
>SPAC30D11.13 |hus5|ubc9|SUMO conjugating enzyme
Hus5|Schizosaccharomyces pombe|chr 1|||Manual
Length = 157
Score = 25.8 bits (54), Expect = 7.2
Identities = 10/20 (50%), Positives = 11/20 (55%)
Frame = -1
Query: 533 WVRRHHGTAYSKPCTCHDGG 474
W R H Y+KPC DGG
Sbjct: 16 WRRDHPFGFYAKPCKSSDGG 35
>SPAC222.10c |byr4||two-component GAP Byr4|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 665
Score = 25.8 bits (54), Expect = 7.2
Identities = 13/34 (38%), Positives = 19/34 (55%), Gaps = 2/34 (5%)
Frame = +3
Query: 549 PSXPSKPSVLSNSST--GVQPVSRSVSTTSHPPW 644
P PS+P+++SN ST G+Q V V + W
Sbjct: 531 PISPSRPALISNISTKKGIQVVGNMVYDPTRLRW 564
>SPAC1B3.15c |||membrane transporter|Schizosaccharomyces pombe|chr
1|||Manual
Length = 628
Score = 25.8 bits (54), Expect = 7.2
Identities = 16/33 (48%), Positives = 20/33 (60%), Gaps = 1/33 (3%)
Frame = +3
Query: 543 TRPSXPS-KPSVLSNSSTGVQPVSRSVSTTSHP 638
T P PS PS+ S+ T V+P S STTS+P
Sbjct: 5 TLPPKPSISPSIASSFPT-VKPFSSQNSTTSNP 36
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,983,009
Number of Sequences: 5004
Number of extensions: 55317
Number of successful extensions: 187
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 154
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 185
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 388424860
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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