Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= br--1891
(749 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPCC794.12c |mae2||malic enzyme|Schizosaccharomyces pombe|chr 3|... 77 4e-15
SPAC750.08c |||NAD-dependent malic enzyme|Schizosaccharomyces po... 54 2e-08
SPBC30D10.15 |||snoRNP assembly factor |Schizosaccharomyces pomb... 29 0.94
SPAC20H4.03c |tfs1||transcription elongation factor TFIIS |Schiz... 28 1.6
SPAC4F8.13c |rng2||IQGAP|Schizosaccharomyces pombe|chr 1|||Manual 27 2.2
SPAC1F5.07c |hem14||protoporphyrinogen oxidase|Schizosaccharomyc... 27 2.9
SPAC15A10.03c |rhp54|rad54|Rad54 homolog Rhp54|Schizosaccharomyc... 26 6.6
SPCC70.05c |||serine/threonine protein kinase |Schizosaccharomyc... 25 8.7
SPBC9B6.08 |clc1||clathrin light chain|Schizosaccharomyces pombe... 25 8.7
>SPCC794.12c |mae2||malic enzyme|Schizosaccharomyces pombe|chr
3|||Manual
Length = 565
Score = 76.6 bits (180), Expect = 4e-15
Identities = 39/78 (50%), Positives = 52/78 (66%)
Frame = +1
Query: 256 FNDDIQGTASVAVAGLMAAVRVTRRKLSENIYLFLGAGSAANGIANLTVAAMMADGLSER 435
FNDDIQGT +VA+A ++ A+ VT+ L+E + GAG+A GIAN VA M+ DGLS
Sbjct: 269 FNDDIQGTGAVALAAIIGALHVTKSPLTEQRIMIFGAGTAGVGIANQIVAGMVTDGLSLD 328
Query: 436 QARERVYMFDVDGLLSTR 489
+AR ++M D GLL R
Sbjct: 329 KARGNLFMIDRCGLLLER 346
Score = 75.8 bits (178), Expect = 6e-15
Identities = 35/80 (43%), Positives = 53/80 (66%)
Frame = +2
Query: 2 AGIKPHQCLPITLDVGTDNQDLLEDPLYIGLRQKRARGKEYDEFIDEFMEACVQRYGQNT 181
AG+ P++ LPI LDVGT+N+ ++ Y+GLR+ R RG++YD F+D ++A ++
Sbjct: 185 AGLDPNRFLPIVLDVGTNNETHRKNHQYMGLRKDRVRGEQYDSFLDNVIKA-IREVFPEA 243
Query: 182 LLQFEDFALPNAGRLLKKYR 241
+ FEDF L NA R+L YR
Sbjct: 244 FIHFEDFGLANAKRILDHYR 263
Score = 57.6 bits (133), Expect = 2e-09
Identities = 23/67 (34%), Positives = 41/67 (61%)
Frame = +3
Query: 549 DFEACVAKIKPSCLIGCSTVGGAFTPNVLKQMAMNTERPVIFALSNPTSKAECTAQDAYD 728
+ E+ +A +KP+ L+GCS G FT +++M+ + ERP+IF +SNPT+ E
Sbjct: 374 NLESAIALVKPTILLGCSGQPGKFTEKAIREMSKHVERPIIFPISNPTTLMEAKPDQIDK 433
Query: 729 HTEGRCI 749
++G+ +
Sbjct: 434 WSDGKAL 440
>SPAC750.08c |||NAD-dependent malic enzyme|Schizosaccharomyces
pombe|chr 1||Partial|Manual
Length = 228
Score = 54.4 bits (125), Expect = 2e-08
Identities = 22/67 (32%), Positives = 39/67 (58%)
Frame = +3
Query: 549 DFEACVAKIKPSCLIGCSTVGGAFTPNVLKQMAMNTERPVIFALSNPTSKAECTAQDAYD 728
D E ++ IKP+ L+GCS G FT +++M+ + + P+IF +SNPT+ E +
Sbjct: 37 DLETAISLIKPTVLLGCSGQPGKFTEKAIREMSKHVKHPIIFPISNPTTLMEAKPVQIDE 96
Query: 729 HTEGRCI 749
+ G+ +
Sbjct: 97 WSNGKAL 103
>SPBC30D10.15 |||snoRNP assembly factor |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 516
Score = 28.7 bits (61), Expect = 0.94
Identities = 11/40 (27%), Positives = 22/40 (55%)
Frame = -1
Query: 500 PPSRRVDNSPSTSNMYTLSRACLSESPSAIMAATVKFAMP 381
PP++R P +S++Y+LS + ++ S + M + P
Sbjct: 423 PPAKRFTQEPPSSSLYSLSESSINYSTQSPMYYNYNYPQP 462
>SPAC20H4.03c |tfs1||transcription elongation factor TFIIS
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 293
Score = 27.9 bits (59), Expect = 1.6
Identities = 14/46 (30%), Positives = 27/46 (58%)
Frame = +2
Query: 413 WRTDSPRGRPARECTCSTSTDYCPLDVKEGFRARQGLREGYRTGER 550
W+ D +GRP + T ++ST DV G +A++ +++ +G+R
Sbjct: 74 WKADVSKGRPLKTTTTTSSTPSKHADV--GSQAQKQVQKQSSSGQR 117
>SPAC4F8.13c |rng2||IQGAP|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1489
Score = 27.5 bits (58), Expect = 2.2
Identities = 14/40 (35%), Positives = 24/40 (60%), Gaps = 4/40 (10%)
Frame = +2
Query: 86 IGLRQKRA----RGKEYDEFIDEFMEACVQRYGQNTLLQF 193
+ LR+KRA + K Y+E+I++ ME + G+ L+ F
Sbjct: 1333 LNLREKRAFLDSQLKSYNEYIEQAMETLQSKKGKKKLIPF 1372
>SPAC1F5.07c |hem14||protoporphyrinogen oxidase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 490
Score = 27.1 bits (57), Expect = 2.9
Identities = 16/40 (40%), Positives = 21/40 (52%)
Frame = -1
Query: 479 NSPSTSNMYTLSRACLSESPSAIMAATVKFAMPFAADPAP 360
NSPS N Y L+E PS+I+ ++K M A P P
Sbjct: 94 NSPSAKNRYIYYPDRLNEIPSSIL-GSIKSIMQPALRPMP 132
>SPAC15A10.03c |rhp54|rad54|Rad54 homolog Rhp54|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 852
Score = 25.8 bits (54), Expect = 6.6
Identities = 14/41 (34%), Positives = 18/41 (43%)
Frame = +3
Query: 531 DIEPEKDFEACVAKIKPSCLIGCSTVGGAFTPNVLKQMAMN 653
D EPE D ++CV+ + CST T N K N
Sbjct: 196 DSEPESDHDSCVS---TDTVASCSTEQSLITSNTSKHRRPN 233
>SPCC70.05c |||serine/threonine protein kinase |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 781
Score = 25.4 bits (53), Expect = 8.7
Identities = 11/30 (36%), Positives = 20/30 (66%)
Frame = +3
Query: 633 LKQMAMNTERPVIFALSNPTSKAECTAQDA 722
+ + M+ +R + F L+NPT++ TAQ+A
Sbjct: 708 VNDLPMHMQR-LFFCLANPTAEQRITAQEA 736
>SPBC9B6.08 |clc1||clathrin light chain|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 229
Score = 25.4 bits (53), Expect = 8.7
Identities = 13/31 (41%), Positives = 17/31 (54%)
Frame = +3
Query: 51 RTIRTF*KILYTSV*DKNAPGERNTTSSSMS 143
R+ F ++L + D NAPG TT SS S
Sbjct: 199 RSTERFRELLISLAKDSNAPGAAGTTVSSSS 229
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,302,842
Number of Sequences: 5004
Number of extensions: 72503
Number of successful extensions: 241
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 232
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 241
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 357280532
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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