Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= br--1864X
(540 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC186.08c |||L-lactate dehydrogenase |Schizosaccharomyces pomb... 80 2e-16
SPBC1539.07c |||glutathione-dependent formaldehyde dehydrogenase... 29 0.58
SPBC1347.04 |tim54||TIM22 inner membrane protein import complex ... 28 1.0
SPAPB1A10.07c |||sphingolipid biosynthesis protein|Schizosacchar... 26 4.1
SPAC821.09 |eng1||endo-1,3-beta-glucanase Eng1|Schizosaccharomyc... 25 5.4
SPAC2E1P3.04 |||copper amine oxidase |Schizosaccharomyces pombe|... 25 7.2
SPCC24B10.20 |||short chain dehydrogenase |Schizosaccharomyces p... 25 7.2
SPBC146.12 |coq6||monooxygenase Coq6|Schizosaccharomyces pombe|c... 25 7.2
SPBC16A3.07c |nrm1||negative regulator of MBF|Schizosaccharomyce... 25 7.2
SPAC664.10 |klp2||kinesin-like protein Klp2|Schizosaccharomyces ... 25 7.2
SPBC28E12.06c |lvs1|SPBC3H7.16|beige protein homolog|Schizosacch... 25 9.5
SPAC631.01c |acp2||F-actin capping protein beta subunit |Schizos... 25 9.5
SPBC32H8.02c |nep2|mug120|nedd8 protease Nep2|Schizosaccharomyce... 25 9.5
>SPAC186.08c |||L-lactate dehydrogenase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 330
Score = 79.8 bits (188), Expect = 2e-16
Identities = 34/79 (43%), Positives = 53/79 (67%)
Frame = +2
Query: 281 DYSITAGSKICVVTAGVRQREGESRLDLVQRNTDVLKQIIPQLIKYSPDTILVIASNPVD 460
DY + V+TAG Q+ GE+R+DL++ N + K+I+ ++ KY+ D IL++A+NPVD
Sbjct: 81 DYKDCKDATAVVITAGKNQKPGETRMDLLKANISIFKEILREVTKYTKDAILLVATNPVD 140
Query: 461 ILTYVTWKISGXPKHRSSG 517
+LTY T K++G P R G
Sbjct: 141 VLTYATLKLTGFPAERVIG 159
Score = 46.0 bits (104), Expect = 4e-06
Identities = 19/48 (39%), Positives = 28/48 (58%)
Frame = +3
Query: 102 KVTIVGVGQVGMAAAFSMLTQNVTNNIALVDMMADKLKGEMMDLQHGS 245
K+ IVG G VG AF++L + I ++D+ K +GE MDL H +
Sbjct: 22 KIVIVGAGNVGSTTAFTLLLSGLAAEIVIIDLNKKKAEGEAMDLNHAA 69
>SPBC1539.07c |||glutathione-dependent formaldehyde dehydrogenase
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 378
Score = 28.7 bits (61), Expect = 0.58
Identities = 17/45 (37%), Positives = 24/45 (53%)
Frame = +3
Query: 75 HEKVDETWSKVTIVGVGQVGMAAAFSMLTQNVTNNIALVDMMADK 209
H E+ S V +VG G VG+AA + + IA +D+ ADK
Sbjct: 187 HSAKVESGSTVAVVGCGCVGLAAMQGAVAAGASRIIA-IDINADK 230
>SPBC1347.04 |tim54||TIM22 inner membrane protein import complex
subunit Tim54|Schizosaccharomyces pombe|chr 2|||Manual
Length = 347
Score = 27.9 bits (59), Expect = 1.0
Identities = 12/48 (25%), Positives = 21/48 (43%)
Frame = -3
Query: 493 PANLPRNIRENIHGVTGDHQYCVRTVLYQLRDYLFKNIGVSLHEIETR 350
P LPR ++ +HG GD Y R + +F + +E++
Sbjct: 56 PLELPRKLKVYLHGPPGDGIYVAREEFEEYIRPIFNAAAIEFETVESK 103
>SPAPB1A10.07c |||sphingolipid biosynthesis
protein|Schizosaccharomyces pombe|chr 1|||Manual
Length = 441
Score = 25.8 bits (54), Expect = 4.1
Identities = 10/21 (47%), Positives = 14/21 (66%)
Frame = -2
Query: 251 EC*SVLQVHHLSFQFVSHHVY 189
+C SV+ VH LSF V H++
Sbjct: 87 KCYSVIAVHRLSFTLVMFHLF 107
>SPAC821.09 |eng1||endo-1,3-beta-glucanase Eng1|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1016
Score = 25.4 bits (53), Expect = 5.4
Identities = 12/40 (30%), Positives = 20/40 (50%)
Frame = -1
Query: 432 IVSGLYFISCGIICLRTSVFLCTRSRRDSPSRCRTPAVTT 313
I+ G+ ++SC C S ++C+ SP+ T TT
Sbjct: 901 IIDGVDYLSCNGACYNPSQYVCSDGSL-SPNTVTTTKATT 939
>SPAC2E1P3.04 |||copper amine oxidase |Schizosaccharomyces pombe|chr
1|||Manual
Length = 712
Score = 25.0 bits (52), Expect = 7.2
Identities = 15/61 (24%), Positives = 29/61 (47%)
Frame = -2
Query: 272 IGSWRSHEC*SVLQVHHLSFQFVSHHVY*SDVIRNILRQHRESCGHPNLTDPDNGHLAPS 93
+G R + SV Q ++F+ ++ + +I +RE +++ DNGH+ P
Sbjct: 235 LGKMREVKPISVTQPEGVNFRMKGRYIEWQNFRMHIGFNYREGIVLSDISYNDNGHIRPL 294
Query: 92 F 90
F
Sbjct: 295 F 295
>SPCC24B10.20 |||short chain dehydrogenase |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 254
Score = 25.0 bits (52), Expect = 7.2
Identities = 18/58 (31%), Positives = 26/58 (44%), Gaps = 2/58 (3%)
Frame = +3
Query: 51 LKKLFQPVHEKVDETW-SKVTIVGVGQVGMAAAF-SMLTQNVTNNIALVDMMADKLKG 218
+ K FQPV + DE W S +G + + AF +L + NI MA + G
Sbjct: 93 ISKSFQPVLKTSDELWMSHYQTNVLGPIHVYQAFYHLLKEGKLKNIVFTSSMAACMGG 150
>SPBC146.12 |coq6||monooxygenase Coq6|Schizosaccharomyces pombe|chr
2|||Manual
Length = 466
Score = 25.0 bits (52), Expect = 7.2
Identities = 16/48 (33%), Positives = 27/48 (56%), Gaps = 2/48 (4%)
Frame = +3
Query: 78 EKVDETWSKVTIVGVGQVGMAAAFSMLTQNVTNN--IALVDMMADKLK 215
+K+ + V IVG G VG+A A + + VT + + L+D+ D +K
Sbjct: 18 QKILQRQFDVVIVGSGPVGLALAAGLQSNPVTQSLKVGLLDIQ-DTMK 64
>SPBC16A3.07c |nrm1||negative regulator of MBF|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 342
Score = 25.0 bits (52), Expect = 7.2
Identities = 10/26 (38%), Positives = 17/26 (65%)
Frame = -3
Query: 334 SNTSSNHADLRASCYRIIRTRLDLGV 257
+NT++NH D C + +R RL+L +
Sbjct: 120 TNTNNNHLDDIQCCAKNLRLRLELAM 145
>SPAC664.10 |klp2||kinesin-like protein Klp2|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 817
Score = 25.0 bits (52), Expect = 7.2
Identities = 12/28 (42%), Positives = 15/28 (53%)
Frame = +2
Query: 428 TILVIASNPVDILTYVTWKISGXPKHRS 511
TI I S P+D VTW + K+RS
Sbjct: 644 TITNITSEPLDTPEQVTWLLDQASKNRS 671
>SPBC28E12.06c |lvs1|SPBC3H7.16|beige protein
homolog|Schizosaccharomyces pombe|chr 2|||Manual
Length = 2609
Score = 24.6 bits (51), Expect = 9.5
Identities = 10/36 (27%), Positives = 19/36 (52%)
Frame = -3
Query: 346 TFALSNTSSNHADLRASCYRIIRTRLDLGVPMNADP 239
+ A+ + SN+ ++ S Y I +DL + N +P
Sbjct: 935 SIAVDRSDSNYISIKESGYAICEEFIDLLIKSNGEP 970
>SPAC631.01c |acp2||F-actin capping protein beta subunit
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 268
Score = 24.6 bits (51), Expect = 9.5
Identities = 11/29 (37%), Positives = 20/29 (68%)
Frame = +2
Query: 380 DVLKQIIPQLIKYSPDTILVIASNPVDIL 466
D+L+++ P+ I + DTIL +A + D+L
Sbjct: 9 DLLRRLNPKDISKNLDTILSVAPDLADVL 37
>SPBC32H8.02c |nep2|mug120|nedd8 protease Nep2|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 415
Score = 24.6 bits (51), Expect = 9.5
Identities = 12/26 (46%), Positives = 17/26 (65%)
Frame = -1
Query: 108 SPCSKFRPPSRARAGTASSGTPSCGS 31
SP K + R+ G++SSG+ SCGS
Sbjct: 39 SPKKKKKGFFRSLFGSSSSGSKSCGS 64
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,301,575
Number of Sequences: 5004
Number of extensions: 46215
Number of successful extensions: 136
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 131
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 136
length of database: 2,362,478
effective HSP length: 69
effective length of database: 2,017,202
effective search space used: 221892220
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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