Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= br--1861
(633 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC18G6.04c |shm2||serine hydroxymethyltransferase Shm2 |Schizo... 75 9e-15
SPAC24C9.12c |||glycine hydroxymethyltransferase |Schizosaccharo... 69 6e-13
SPAC144.14 |klp8||kinesin-like protein Klp8|Schizosaccharomyces ... 27 1.7
SPCC1450.14c |ero12||ER oxidoreductin Ero1b|Schizosaccharomyces ... 27 3.0
SPAC1F3.05 |||adaptin |Schizosaccharomyces pombe|chr 1|||Manual 27 3.0
SPAC1006.04c |mcp3|mug7|sequence orphan|Schizosaccharomyces pomb... 25 6.9
SPAC11E3.08c |nse6||Smc5-6 complex non-SMC subunit Nse6|Schizosa... 25 6.9
SPAC2G11.02 |urb2||ribosome biogenesis protein Urb2 |Schizosacch... 25 9.1
SPAC167.01 |ppk4||serine/threonine protein kinase Ppk4 |Schizosa... 25 9.1
>SPAC18G6.04c |shm2||serine hydroxymethyltransferase Shm2
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 472
Score = 74.9 bits (176), Expect = 9e-15
Identities = 34/66 (51%), Positives = 44/66 (66%)
Frame = +3
Query: 315 KLLNSNLWEADPELFDIIVKEKDRQRAGLEMIASDNFTSVPVLQCLSSCLHNKYSEGMPN 494
KLL + L E DP ++ I+ EK RQ+ + +IAS+NFTS V+ L S + NKYSEG P
Sbjct: 12 KLLKAPLAECDPTVYKILESEKSRQKESIALIASENFTSRAVMDALGSIMQNKYSEGYPG 71
Query: 495 QRYYGG 512
RYYGG
Sbjct: 72 ARYYGG 77
Score = 51.6 bits (118), Expect = 9e-08
Identities = 22/32 (68%), Positives = 26/32 (81%)
Frame = +1
Query: 532 EILAQNRSLEAYRLKSEEWGVNVQPYSGSPAN 627
E L Q R+LEA+ L E+WGVNVQP+SGSPAN
Sbjct: 85 ERLCQTRALEAFHLDGEKWGVNVQPHSGSPAN 116
>SPAC24C9.12c |||glycine hydroxymethyltransferase
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 467
Score = 68.9 bits (161), Expect = 6e-13
Identities = 33/69 (47%), Positives = 44/69 (63%)
Frame = +3
Query: 333 LWEADPELFDIIVKEKDRQRAGLEMIASDNFTSVPVLQCLSSCLHNKYSEGMPNQRYYGG 512
L E DP + +I+ E DRQR+ + +IAS+NFTS V+ L S + NKYSEG P RYYGG
Sbjct: 12 LKEQDPTVAEIMRHEADRQRSSVVLIASENFTSRAVMDALGSVMSNKYSEGYPGARYYGG 71
Query: 513 MNILMDRDT 539
+ +T
Sbjct: 72 NKFIDQIET 80
Score = 43.6 bits (98), Expect = 2e-05
Identities = 20/33 (60%), Positives = 23/33 (69%)
Frame = +1
Query: 529 IEILAQNRSLEAYRLKSEEWGVNVQPYSGSPAN 627
IE L Q R+L A+ L +WGVNVQ SGSPAN
Sbjct: 78 IETLCQERALAAFNLDPAKWGVNVQCLSGSPAN 110
>SPAC144.14 |klp8||kinesin-like protein Klp8|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 511
Score = 27.5 bits (58), Expect = 1.7
Identities = 19/56 (33%), Positives = 28/56 (50%), Gaps = 6/56 (10%)
Frame = +3
Query: 243 CVIGCYLFETFAAKRYISSTKMSAKLLNSNLWEA-----DPELFDIIVK-EKDRQR 392
CV C +TF+A RY + + + N N EA + EL DI+ E DR++
Sbjct: 334 CVSPCDFDDTFSALRYSEAARRIKNISNINCKEAYSTNNEGELDDILTTLESDREQ 389
>SPCC1450.14c |ero12||ER oxidoreductin Ero1b|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 571
Score = 26.6 bits (56), Expect = 3.0
Identities = 11/25 (44%), Positives = 14/25 (56%)
Frame = -1
Query: 519 YSFPHSTVDWACLQNICYEDMNSGT 445
YS PHST W + N C D ++ T
Sbjct: 196 YSGPHSTRIWEMIYNQCLPDSSAPT 220
>SPAC1F3.05 |||adaptin |Schizosaccharomyces pombe|chr 1|||Manual
Length = 510
Score = 26.6 bits (56), Expect = 3.0
Identities = 15/35 (42%), Positives = 21/35 (60%)
Frame = -3
Query: 412 AIISSPAR*RSFSLTIISKSSGSASQRLLFSNLAL 308
+I+ S + S LT + KSSG AS + FSN +L
Sbjct: 397 SILQSDSLMISTQLTSVQKSSGFASYSVQFSNCSL 431
>SPAC1006.04c |mcp3|mug7|sequence orphan|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 952
Score = 25.4 bits (53), Expect = 6.9
Identities = 16/40 (40%), Positives = 22/40 (55%)
Frame = -2
Query: 269 LKQITADNAIASS*LLSTIRSFLTL*ENVRFFNTMKLVNY 150
L + + DN S LLS +SF TL E ++ N M VN+
Sbjct: 148 LYKFSLDNQTFSQ-LLSRFKSFATLTELLQVHNVMLQVNF 186
>SPAC11E3.08c |nse6||Smc5-6 complex non-SMC subunit
Nse6|Schizosaccharomyces pombe|chr 1|||Manual
Length = 522
Score = 25.4 bits (53), Expect = 6.9
Identities = 9/36 (25%), Positives = 22/36 (61%)
Frame = +3
Query: 519 ILMDRDTGTEQVSGSIQIKIGRMGRQCTAIFRFSGE 626
I ++R + +E + +++IK+ + C ++RFS +
Sbjct: 250 IQLERTSASENLKSNLKIKVINFLKCCGTLYRFSDD 285
>SPAC2G11.02 |urb2||ribosome biogenesis protein Urb2
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1318
Score = 25.0 bits (52), Expect = 9.1
Identities = 6/17 (35%), Positives = 13/17 (76%)
Frame = -1
Query: 510 PHSTVDWACLQNICYED 460
PHS + W C++++ ++D
Sbjct: 379 PHSKILWECIKSVSHQD 395
>SPAC167.01 |ppk4||serine/threonine protein kinase Ppk4
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1072
Score = 25.0 bits (52), Expect = 9.1
Identities = 16/52 (30%), Positives = 28/52 (53%)
Frame = +3
Query: 369 VKEKDRQRAGLEMIASDNFTSVPVLQCLSSCLHNKYSEGMPNQRYYGGMNIL 524
V+E+D L+M+ +N SV + + ++CLH+ + + R Y G IL
Sbjct: 957 VEERDPPSPLLQML-ENNSKSV-IGENWTTCLHSSLVDNLGKYRKYDGSKIL 1006
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,496,590
Number of Sequences: 5004
Number of extensions: 50545
Number of successful extensions: 142
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 136
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 142
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 281707720
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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