Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= br--1844X
(536 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 154 9e-39
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 152 3e-38
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 99 4e-22
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 69 4e-13
SPBC8D2.17 |||alpha-1,2-galactosyltransferase|Schizosaccharomyce... 29 0.44
SPCC18.01c |adg3|SPCC74.07c|beta-glucosidase Adg3 |Schizosacchar... 28 0.77
SPCC1742.01 ||SPCC1795.13, SPCPB16A4.07c|sequence orphan|Schizos... 28 1.0
SPAC17C9.03 |tif471||translation initiation factor eIF4G |Schizo... 26 3.1
SPCC18.08 |||lysine-tRNA ligase|Schizosaccharomyces pombe|chr 3|... 26 3.1
SPAC23H3.15c ||SPAC25H1.01c|sequence orphan|Schizosaccharomyces ... 26 3.1
SPBC14F5.10c |||ubiquitin-protein ligase E3 |Schizosaccharomyces... 26 4.1
SPAC9E9.05 |||sequence orphan|Schizosaccharomyces pombe|chr 1|||... 25 5.4
SPBC409.09c |mis13|cnl1|kinetochore protein Mis13|Schizosaccharo... 25 5.4
SPAP7G5.04c |lys1||aminoadipate-semialdehyde dehydrogenase |Schi... 25 5.4
SPAC22E12.11c |set3||histone lysine methyltransferase Set3|Schiz... 25 7.2
SPBC21B10.13c |||transcription factor, homeobox type |Schizosacc... 25 9.5
SPAC19A8.15 |trp2||tryptophan synthase|Schizosaccharomyces pombe... 25 9.5
SPCC4G3.08 |psk1||serine/threonine protein kinase Psk1|Schizosac... 25 9.5
SPAC227.01c ||SPAPB21F2.04c|Erd1 homolog|Schizosaccharomyces pom... 25 9.5
>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 449
Score = 154 bits (373), Expect = 9e-39
Identities = 65/88 (73%), Positives = 79/88 (89%)
Frame = +1
Query: 241 RGKARPRAVFVDLEPTVVDEVRTGTYRQLFHPDQLITGKEDAANNYARGHYTIGKEIVDV 420
+GK PR+++VDLEP V+D+VRTG YR LFHP+QLITGKEDA+NNYARGHYT+GKE+VD
Sbjct: 58 QGKYVPRSIYVDLEPNVIDQVRTGPYRDLFHPEQLITGKEDASNNYARGHYTVGKELVDE 117
Query: 421 VLDRITKLADQCTGLQGFLVFHSFGGGT 504
V D+I ++AD C+GLQGFLVFHSFGGGT
Sbjct: 118 VTDKIRRIADNCSGLQGFLVFHSFGGGT 145
Score = 97.5 bits (232), Expect = 1e-21
Identities = 41/61 (67%), Positives = 47/61 (77%)
Frame = +2
Query: 71 MRECISVHIGQAGVQIGNACWELYCLEHGIQPDGQMPSDKTLGGGDDSFNTFFSETGAGK 250
MRE IS+H+GQAG QIGNACWELYCLEHGIQP+G M + D F+TFFSETG GK
Sbjct: 1 MREIISIHVGQAGTQIGNACWELYCLEHGIQPNGYMNPETASQNSDGGFSTFFSETGQGK 60
Query: 251 H 253
+
Sbjct: 61 Y 61
>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 455
Score = 152 bits (369), Expect = 3e-38
Identities = 62/88 (70%), Positives = 80/88 (90%)
Frame = +1
Query: 241 RGKARPRAVFVDLEPTVVDEVRTGTYRQLFHPDQLITGKEDAANNYARGHYTIGKEIVDV 420
+GK PR+++VDLEP V+D+VRTG Y+ LFHP+Q++TGKEDA+NNYARGHYT+GKE++D
Sbjct: 62 QGKFVPRSIYVDLEPNVIDQVRTGPYKDLFHPEQMVTGKEDASNNYARGHYTVGKEMIDS 121
Query: 421 VLDRITKLADQCTGLQGFLVFHSFGGGT 504
VL+RI ++AD C+GLQGFLVFHSFGGGT
Sbjct: 122 VLERIRRMADNCSGLQGFLVFHSFGGGT 149
Score = 89.0 bits (211), Expect = 4e-19
Identities = 44/65 (67%), Positives = 48/65 (73%), Gaps = 5/65 (7%)
Frame = +2
Query: 71 MRECISVHIGQAGVQIGNACWELYCLEHGIQPDGQMPSD-----KTLGGGDDSFNTFFSE 235
MRE ISVH+GQAGVQIGNACWELYCLEHGI PDG P++ K +D F TFFSE
Sbjct: 1 MREVISVHVGQAGVQIGNACWELYCLEHGIGPDG-FPTENSEVHKNNSYLNDGFGTFFSE 59
Query: 236 TGAGK 250
TG GK
Sbjct: 60 TGQGK 64
>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 448
Score = 99.1 bits (236), Expect = 4e-22
Identities = 45/87 (51%), Positives = 58/87 (66%)
Frame = +1
Query: 244 GKARPRAVFVDLEPTVVDEVRTGTYRQLFHPDQLITGKEDAANNYARGHYTIGKEIVDVV 423
GK PRAV VDLEP +D V++G + LF PD +I G+ A N +A+GHYT G E+ D V
Sbjct: 57 GKYVPRAVLVDLEPGTMDAVKSGKFGNLFRPDNIIYGQSGAGNIWAKGHYTEGAELADAV 116
Query: 424 LDRITKLADQCTGLQGFLVFHSFGGGT 504
LD + + A+ C LQGF + HS GGGT
Sbjct: 117 LDVVRREAEACDALQGFQLTHSLGGGT 143
Score = 39.9 bits (89), Expect = 2e-04
Identities = 20/61 (32%), Positives = 28/61 (45%)
Frame = +2
Query: 71 MRECISVHIGQAGVQIGNACWELYCLEHGIQPDGQMPSDKTLGGGDDSFNTFFSETGAGK 250
MRE + + GQ G Q+G A W EHG+ G T + N +F+E GK
Sbjct: 1 MREIVHIQAGQCGNQVGAAFWSTIADEHGLDSAGIYHG--TSEAQHERLNVYFNEAAGGK 58
Query: 251 H 253
+
Sbjct: 59 Y 59
>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 446
Score = 68.9 bits (161), Expect = 4e-13
Identities = 33/85 (38%), Positives = 52/85 (61%), Gaps = 2/85 (2%)
Frame = +1
Query: 256 PRAVFVDLEPTVVDEVRTGTYRQLFHPDQLITGKE--DAANNYARGHYTIGKEIVDVVLD 429
PRA+ +DLEP VV+ + + TY L++P+ ++ K A NN+A G Y+ + I + ++D
Sbjct: 62 PRAILIDLEPRVVNNILSDTYGSLYNPENILITKNGGGAGNNWANG-YSHAERIFEDIMD 120
Query: 430 RITKLADQCTGLQGFLVFHSFGGGT 504
I + AD L+GF + HS GGT
Sbjct: 121 MIDREADGSDSLEGFSLLHSIAGGT 145
Score = 49.6 bits (113), Expect = 3e-07
Identities = 25/55 (45%), Positives = 33/55 (60%)
Frame = +2
Query: 74 RECISVHIGQAGVQIGNACWELYCLEHGIQPDGQMPSDKTLGGGDDSFNTFFSET 238
RE I++ GQ G QIG+ W+ CLEHGI PDG + S T G D + FF ++
Sbjct: 3 REIITLQAGQCGNQIGSQFWQQLCLEHGIGPDGTLESFAT--EGVDRKDVFFYQS 55
>SPBC8D2.17 |||alpha-1,2-galactosyltransferase|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 351
Score = 29.1 bits (62), Expect = 0.44
Identities = 13/34 (38%), Positives = 20/34 (58%)
Frame = -3
Query: 516 VNPSRSTSERVKHEETLKPCALVSQLRDPVQNYV 415
+NPS S +RV E L L++ DP++NY+
Sbjct: 154 MNPSESLKDRVLKPEKLSQHLLLNSPIDPLKNYI 187
>SPCC18.01c |adg3|SPCC74.07c|beta-glucosidase Adg3
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 1131
Score = 28.3 bits (60), Expect = 0.77
Identities = 13/49 (26%), Positives = 24/49 (48%)
Frame = +3
Query: 117 LATPAGSCTASSMESSLMVRCRQIRPSAVETTPLTRSSARPARESTATS 263
++TPA + S+ + + PS+ +TTP + SS ++ TS
Sbjct: 618 VSTPASNSNTGSLNGTSSFNVNSVGPSSSQTTPTSSSSITGSQSLKETS 666
>SPCC1742.01 ||SPCC1795.13, SPCPB16A4.07c|sequence
orphan|Schizosaccharomyces pombe|chr 3|||Manual
Length = 1563
Score = 27.9 bits (59), Expect = 1.0
Identities = 10/25 (40%), Positives = 18/25 (72%)
Frame = +3
Query: 450 PMHRASGFPRVSLVRRWNGSDSHHF 524
P+++ SG S VR+WNGS ++++
Sbjct: 1493 PIYQGSGTSTWSTVRQWNGSATYNY 1517
>SPAC17C9.03 |tif471||translation initiation factor eIF4G
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1403
Score = 26.2 bits (55), Expect = 3.1
Identities = 14/59 (23%), Positives = 28/59 (47%)
Frame = +3
Query: 78 NASPFTSVKPAYRLATPAGSCTASSMESSLMVRCRQIRPSAVETTPLTRSSARPAREST 254
N F+ + + + T +S ++ + ++PS VE T +R++A+ EST
Sbjct: 8 NTPKFSYARALASSQSNKSNSTKASENNTATAEKQAVKPSGVEPTNTSRANAQKKTEST 66
>SPCC18.08 |||lysine-tRNA ligase|Schizosaccharomyces pombe|chr
3|||Manual
Length = 531
Score = 26.2 bits (55), Expect = 3.1
Identities = 11/18 (61%), Positives = 13/18 (72%)
Frame = +1
Query: 475 LVFHSFGGGTARIHITSD 528
++ H FGG TAR ITSD
Sbjct: 230 ILSHHFGGATARPFITSD 247
>SPAC23H3.15c ||SPAC25H1.01c|sequence orphan|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 325
Score = 26.2 bits (55), Expect = 3.1
Identities = 13/28 (46%), Positives = 17/28 (60%)
Frame = +2
Query: 179 PSDKTLGGGDDSFNTFFSETGAGKHGHE 262
PSD T+GGG +++ S T G HG E
Sbjct: 191 PSD-TVGGGAYDYSSSGSHTHGGSHGTE 217
>SPBC14F5.10c |||ubiquitin-protein ligase E3 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 486
Score = 25.8 bits (54), Expect = 4.1
Identities = 7/24 (29%), Positives = 17/24 (70%)
Frame = +1
Query: 373 NYARGHYTIGKEIVDVVLDRITKL 444
N+ + HY++ E+ DV++ ++ +L
Sbjct: 64 NHKKPHYSVETEVKDVIISKVVEL 87
>SPAC9E9.05 |||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 313
Score = 25.4 bits (53), Expect = 5.4
Identities = 12/37 (32%), Positives = 20/37 (54%)
Frame = +2
Query: 263 LSLLIWNQL*STRFVPALTVNCSTPISSSLAKRMLPI 373
LSLL + L + P+ N S P+ S +R++P+
Sbjct: 146 LSLLSTSSLQQRKITPSNLSNTSKPMDSKQLERLIPV 182
>SPBC409.09c |mis13|cnl1|kinetochore protein
Mis13|Schizosaccharomyces pombe|chr 2|||Manual
Length = 329
Score = 25.4 bits (53), Expect = 5.4
Identities = 12/27 (44%), Positives = 15/27 (55%)
Frame = -3
Query: 303 NLVDYSWFQINKDSSWPCFPAPVSLKN 223
N V SW+Q D+ P P P +LKN
Sbjct: 155 NKVSVSWYQRPPDAVIPNKPHPQNLKN 181
>SPAP7G5.04c |lys1||aminoadipate-semialdehyde dehydrogenase
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1419
Score = 25.4 bits (53), Expect = 5.4
Identities = 13/53 (24%), Positives = 25/53 (47%)
Frame = +2
Query: 230 SETGAGKHGHELSLLIWNQL*STRFVPALTVNCSTPISSSLAKRMLPITMPED 388
S+T + L W++ ++ + L + S P+ S L + + T+PED
Sbjct: 2 SQTAPSDTEYNQRLERWSERLKSQTISHLPTDYSRPVPSRLVEAVFERTLPED 54
>SPAC22E12.11c |set3||histone lysine methyltransferase
Set3|Schizosaccharomyces pombe|chr 1|||Manual
Length = 859
Score = 25.0 bits (52), Expect = 7.2
Identities = 17/51 (33%), Positives = 23/51 (45%), Gaps = 2/51 (3%)
Frame = +3
Query: 210 TPLTRSSARPARESTATSCLC*FGTNCSRRGSYRH--LPSTVPPRSAHHWQ 356
+P +S R + A L TN S GSYR+ +T+P A H Q
Sbjct: 96 SPTHSASPRQGNNTGANGALFSQSTNSSNSGSYRNSVTGATLPNAHAPHSQ 146
>SPBC21B10.13c |||transcription factor, homeobox type
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 201
Score = 24.6 bits (51), Expect = 9.5
Identities = 12/32 (37%), Positives = 17/32 (53%), Gaps = 1/32 (3%)
Frame = +2
Query: 443 WLTNA-QGFRVSSCFTRSEVERLGFTSLLMER 535
W N Q R S+C +R+ +E G SLL +
Sbjct: 82 WFQNKRQSLRRSNCLSRNRLEGTGENSLLRRK 113
>SPAC19A8.15 |trp2||tryptophan synthase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 697
Score = 24.6 bits (51), Expect = 9.5
Identities = 11/34 (32%), Positives = 19/34 (55%)
Frame = -2
Query: 298 RRLQLVPNQQRQLVAVLSRAGLAEERVKGVVSTA 197
RR++L+ + + V+SR G GV++TA
Sbjct: 162 RRMELLASVADSFIYVVSRMGSTGSSATGVINTA 195
>SPCC4G3.08 |psk1||serine/threonine protein kinase
Psk1|Schizosaccharomyces pombe|chr 3|||Manual
Length = 436
Score = 24.6 bits (51), Expect = 9.5
Identities = 9/21 (42%), Positives = 13/21 (61%)
Frame = +1
Query: 85 LRSHRSSRRTDWQRLLGAVLP 147
++ HR RR DW +L +LP
Sbjct: 344 IKKHRIYRRIDWNKLEKRMLP 364
>SPAC227.01c ||SPAPB21F2.04c|Erd1 homolog|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 373
Score = 24.6 bits (51), Expect = 9.5
Identities = 10/31 (32%), Positives = 17/31 (54%)
Frame = -3
Query: 315 SAGTNLVDYSWFQINKDSSWPCFPAPVSLKN 223
S+ N W+ ++ D S P F P+S++N
Sbjct: 252 SSAINSTYSFWWDVSMDWSLPFFKQPLSIQN 282
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,238,647
Number of Sequences: 5004
Number of extensions: 45915
Number of successful extensions: 141
Number of sequences better than 10.0: 19
Number of HSP's better than 10.0 without gapping: 127
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 139
length of database: 2,362,478
effective HSP length: 68
effective length of database: 2,022,206
effective search space used: 222442660
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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