Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= br--1834
(710 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC24C9.12c |||glycine hydroxymethyltransferase |Schizosaccharo... 124 1e-29
SPAC18G6.04c |shm2||serine hydroxymethyltransferase Shm2 |Schizo... 120 2e-28
SPAC688.10 |rev3||DNA polymerase zeta catalytic subunit Rev3|Sch... 30 0.38
SPAC167.01 |ppk4||serine/threonine protein kinase Ppk4 |Schizosa... 29 0.66
SPAC4A8.12c |sds22||protein phosphatase regulatory subunit Sds22... 27 2.0
SPAC16A10.01 |||DUF1212 family protein|Schizosaccharomyces pombe... 27 3.5
SPBC4F6.12 |||LIM domain|Schizosaccharomyces pombe|chr 2|||Manual 27 3.5
SPBC16H5.11c |skb1|rmt5|type II protein arginine N-methyltransfe... 26 4.6
SPAC30.01c |sec72|sec7b|Sec7 domain|Schizosaccharomyces pombe|ch... 26 4.6
SPBC11G11.07 ||SPBC18H10.01|karyopherin|Schizosaccharomyces pomb... 26 6.1
SPBC577.06c |||phosphatidylinositol kinase |Schizosaccharomyces ... 26 6.1
SPAC3C7.03c |rhp55||RecA family ATPase Rhp55|Schizosaccharomyces... 25 8.1
>SPAC24C9.12c |||glycine hydroxymethyltransferase
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 467
Score = 124 bits (299), Expect = 1e-29
Identities = 53/84 (63%), Positives = 66/84 (78%)
Frame = +3
Query: 255 NFAVYTGIVEPHGRIMGLDLPDGGHLTHGFFTATKKISATSIFFESMPYKVDPKSGLIDY 434
N VY I+ PHGR+MGLDLP GGHL+HG+ T TKKISA S +FESMPY+VDP +GLIDY
Sbjct: 110 NMQVYQAIMPPHGRLMGLDLPSGGHLSHGYQTDTKKISAVSTYFESMPYRVDPNTGLIDY 169
Query: 435 DKLAETAKLFKPRLIIAGMSCYSR 506
D L A+LF+P++++AG S Y R
Sbjct: 170 DMLEHDAQLFRPKILVAGTSAYCR 193
Score = 111 bits (266), Expect = 1e-25
Identities = 52/95 (54%), Positives = 65/95 (68%)
Frame = +1
Query: 4 EKDRQRAGLEMIASENFTSVPVLQCLSSCLHNKYSEGMPNQRYYGGNEYIDEIEILAQNR 183
E DRQR+ + +IASENFTS V+ L S + NKYSEG P RYYGGN++ID+IE L Q R
Sbjct: 26 EADRQRSSVVLIASENFTSRAVMDALGSVMSNKYSEGYPGARYYGGNKFIDQIETLCQER 85
Query: 184 SLEAYRLKSEEWGVNVQPYSGSPATLPFIPALSNP 288
+L A+ L +WGVNVQ SGSPA + A+ P
Sbjct: 86 ALAAFNLDPAKWGVNVQCLSGSPANMQVYQAIMPP 120
Score = 106 bits (255), Expect = 3e-24
Identities = 45/65 (69%), Positives = 57/65 (87%)
Frame = +2
Query: 503 KMLDYKRFREIADANGAYLMADMAHVSGLVAAGVIPSPFEYCDIVTTTTHKTLRGPRAGV 682
+++DY R R+IAD+ AYL+ DMAH+SGLV+AGVIPSPFEY D+VTTTTHK+LRGPR +
Sbjct: 193 RLIDYARMRQIADSVNAYLVVDMAHISGLVSAGVIPSPFEYADVVTTTTHKSLRGPRGAM 252
Query: 683 IFFRK 697
IFFR+
Sbjct: 253 IFFRR 257
>SPAC18G6.04c |shm2||serine hydroxymethyltransferase Shm2
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 472
Score = 120 bits (289), Expect = 2e-28
Identities = 55/95 (57%), Positives = 67/95 (70%)
Frame = +1
Query: 4 EKDRQRAGLEMIASENFTSVPVLQCLSSCLHNKYSEGMPNQRYYGGNEYIDEIEILAQNR 183
EK RQ+ + +IASENFTS V+ L S + NKYSEG P RYYGGNE+ID+ E L Q R
Sbjct: 32 EKSRQKESIALIASENFTSRAVMDALGSIMQNKYSEGYPGARYYGGNEFIDQAERLCQTR 91
Query: 184 SLEAYRLKSEEWGVNVQPYSGSPATLPFIPALSNP 288
+LEA+ L E+WGVNVQP+SGSPA L A+ P
Sbjct: 92 ALEAFHLDGEKWGVNVQPHSGSPANLQAYQAVMKP 126
Score = 106 bits (254), Expect = 4e-24
Identities = 46/65 (70%), Positives = 56/65 (86%)
Frame = +2
Query: 503 KMLDYKRFREIADANGAYLMADMAHVSGLVAAGVIPSPFEYCDIVTTTTHKTLRGPRAGV 682
+++DYKR R+I + AYL+ DMAH+SGLVAAGVIPSPFEY DIVTTTTHK+LRGPR +
Sbjct: 199 RLVDYKRMRKITEMCNAYLLCDMAHISGLVAAGVIPSPFEYADIVTTTTHKSLRGPRGAM 258
Query: 683 IFFRK 697
IF+RK
Sbjct: 259 IFYRK 263
Score = 105 bits (253), Expect = 5e-24
Identities = 44/84 (52%), Positives = 61/84 (72%)
Frame = +3
Query: 255 NFAVYTGIVEPHGRIMGLDLPDGGHLTHGFFTATKKISATSIFFESMPYKVDPKSGLIDY 434
N Y +++PH R+MGLDLP GGHL+HGF T K ISA S +F +MPY V+ ++G+IDY
Sbjct: 116 NLQAYQAVMKPHDRLMGLDLPHGGHLSHGFSTPQKAISAVSTYFSTMPYNVNKETGIIDY 175
Query: 435 DKLAETAKLFKPRLIIAGMSCYSR 506
D L + A F+P++I+AG S Y+R
Sbjct: 176 DSLEKAAIQFRPKVIVAGASAYAR 199
>SPAC688.10 |rev3||DNA polymerase zeta catalytic subunit
Rev3|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1480
Score = 29.9 bits (64), Expect = 0.38
Identities = 17/53 (32%), Positives = 27/53 (50%), Gaps = 4/53 (7%)
Frame = +3
Query: 387 ESMPYKVDPKSGLIDYDKLAETAKLFKPRLIIAGMSCYSRC----SIIRGSVR 533
E++P ++PKS L + + + P +IIA CYS C I+ G V+
Sbjct: 934 EALPLVMEPKSDLYNNPVVVLDFQSLYPSIIIAYNLCYSTCLGPVKIVNGKVK 986
>SPAC167.01 |ppk4||serine/threonine protein kinase Ppk4
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1072
Score = 29.1 bits (62), Expect = 0.66
Identities = 15/61 (24%), Positives = 32/61 (52%)
Frame = +1
Query: 1 KEKDRQRAGLEMIASENFTSVPVLQCLSSCLHNKYSEGMPNQRYYGGNEYIDEIEILAQN 180
+E+D L+M+ EN + + + ++CLH+ + + R Y G++ +D + +L
Sbjct: 958 EERDPPSPLLQML--ENNSKSVIGENWTTCLHSSLVDNLGKYRKYDGSKILDILRVLRNK 1015
Query: 181 R 183
R
Sbjct: 1016 R 1016
>SPAC4A8.12c |sds22||protein phosphatase regulatory subunit Sds22
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 332
Score = 27.5 bits (58), Expect = 2.0
Identities = 18/54 (33%), Positives = 25/54 (46%), Gaps = 2/54 (3%)
Frame = +1
Query: 16 QRAGLEMIASENFTSVPVLQCLSSCLHNKY--SEGMPNQRYYGGNEYIDEIEIL 171
Q+ L I S T L CLS CL Y G+ + + G E ++ +EIL
Sbjct: 192 QKLSLLSIQSNRITQFENLACLSHCLRELYVSHNGLTS---FSGIEVLENLEIL 242
>SPAC16A10.01 |||DUF1212 family protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 830
Score = 26.6 bits (56), Expect = 3.5
Identities = 16/43 (37%), Positives = 23/43 (53%), Gaps = 2/43 (4%)
Frame = -2
Query: 457 FAVSASLS*SINPLLGSTLYGMLSKNIDVAD--IFLVAVKKPW 335
+AV SL S +GS LYG + K+ AD + ++AV W
Sbjct: 624 YAVIFSLFLSFGITIGSALYGWMDKDATDADTCMSIIAVSPYW 666
>SPBC4F6.12 |||LIM domain|Schizosaccharomyces pombe|chr 2|||Manual
Length = 438
Score = 26.6 bits (56), Expect = 3.5
Identities = 10/32 (31%), Positives = 14/32 (43%)
Frame = +2
Query: 230 YSHIQALRQLCRLYRHCRTPWQDNGVRFT*RW 325
Y H+ Q +HC+TP +D V W
Sbjct: 305 YCHLDYHEQFSPRCKHCKTPIEDQAVHINNDW 336
>SPBC16H5.11c |skb1|rmt5|type II protein arginine
N-methyltransferase Skb1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 645
Score = 26.2 bits (55), Expect = 4.6
Identities = 13/52 (25%), Positives = 26/52 (50%)
Frame = -2
Query: 265 TAKLPESLNMAVH*RPILPILICMLPETCSVPVSLFHQYIHSPHSTVDWACL 110
T K+ +++ A P L + + LP CS P+ L +++ P + +C+
Sbjct: 175 TWKMWDTIRSACGYHPRLKVAL-ELPPACSPPIELVNRWYAEPIEMITMSCM 225
>SPAC30.01c |sec72|sec7b|Sec7 domain|Schizosaccharomyces pombe|chr
1|||Manual
Length = 1822
Score = 26.2 bits (55), Expect = 4.6
Identities = 14/55 (25%), Positives = 32/55 (58%)
Frame = -2
Query: 211 PILICMLPETCSVPVSLFHQYIHSPHSTVDWACLQNICYEDMNSGTEVQELM*NF 47
P++ +L ++P+ LF + IH T+ ++C I E+++ +++EL+ N+
Sbjct: 1758 PVIATILDSILNLPLELFSENIH----TLYFSCCSMIAKENLDD--QLRELLKNY 1806
>SPBC11G11.07 ||SPBC18H10.01|karyopherin|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 955
Score = 25.8 bits (54), Expect = 6.1
Identities = 14/41 (34%), Positives = 22/41 (53%)
Frame = -2
Query: 391 LSKNIDVADIFLVAVKKPWVRCPPSGKSNPIILPWGSTMPV 269
L+ NID+ IFL V P P+G+ +PI+ + P+
Sbjct: 620 LADNIDIVTIFLSLVHPP----SPAGELHPIVRLFQDIWPI 656
>SPBC577.06c |||phosphatidylinositol kinase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 1877
Score = 25.8 bits (54), Expect = 6.1
Identities = 16/51 (31%), Positives = 27/51 (52%), Gaps = 1/51 (1%)
Frame = +3
Query: 258 FAVYTGIVEPHGRIMG-LDLPDGGHLTHGFFTATKKISATSIFFESMPYKV 407
++V T +++ R G + + D GH+ H F I+ I FES P+K+
Sbjct: 1720 YSVITYLLQFKDRHNGNIMIDDQGHILHIDFGFIFDIAPGGITFESAPFKL 1770
>SPAC3C7.03c |rhp55||RecA family ATPase Rhp55|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 350
Score = 25.4 bits (53), Expect = 8.1
Identities = 10/23 (43%), Positives = 14/23 (60%)
Frame = -1
Query: 602 HLQQPVQIREPYQPXDKLHLRLR 534
+L P+Q+ P P D +LRLR
Sbjct: 150 NLSMPIQLAYPTSPEDYAYLRLR 172
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,075,748
Number of Sequences: 5004
Number of extensions: 66168
Number of successful extensions: 198
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 190
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 198
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 331187010
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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