BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= br--1782X
(330 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC20G4.06c |adf1|cof1|cofilin|Schizosaccharomyces pombe|chr 1|... 52 2e-08
SPAC9.07c |||GTPase Rbg1 |Schizosaccharomyces pombe|chr 1|||Manual 25 2.2
SPBP35G2.03c |sgo1||shugoshin Sgo1|Schizosaccharomyces pombe|chr... 25 3.0
SPAC6F6.12 |||autophagy associated protein Atg24|Schizosaccharom... 25 3.9
SPAC343.15 |||tRNA isopentenyltransferase|Schizosaccharomyces po... 25 3.9
SPAC23C4.05c |||LEA domain protein|Schizosaccharomyces pombe|chr... 24 5.2
SPAC23D3.14c |aah2||alpha-amylase homolog Aah2|Schizosaccharomyc... 24 5.2
SPAC12B10.16c |mug157||conserved protein |Schizosaccharomyces po... 24 5.2
SPBC3B9.17 |isa2||iron-sulfur protein Isa2|Schizosaccharomyces p... 24 6.8
SPAC637.12c |mst1||histone acetyltransferase Mst1|Schizosaccharo... 24 6.8
SPAC26F1.14c |aif1|SPAC29A4.01c|apoptosis-inducing factor homolo... 23 9.0
SPAC821.06 |spn2||septin Spn2|Schizosaccharomyces pombe|chr 1|||... 23 9.0
>SPAC20G4.06c |adf1|cof1|cofilin|Schizosaccharomyces pombe|chr
1|||Manual
Length = 137
Score = 52.0 bits (119), Expect = 2e-08
Identities = 27/71 (38%), Positives = 40/71 (56%)
Frame = +3
Query: 33 SGVTVSDACKTTYEEIKNDKKHRYVVFYIRDEKQIDVETVGERNAEYEQFLEDLQKGGTG 212
SGV VS C ++E+K K RYVVF + D K V + +++ FL DL +
Sbjct: 4 SGVKVSPECLEAFQELKLGKSLRYVVFKMNDTKTEIVVEKKSTDKDFDTFLGDLPE---K 60
Query: 213 ECRYGLFDFEY 245
+CRY ++DFE+
Sbjct: 61 DCRYAIYDFEF 71
>SPAC9.07c |||GTPase Rbg1 |Schizosaccharomyces pombe|chr 1|||Manual
Length = 366
Score = 25.4 bits (53), Expect = 2.2
Identities = 10/22 (45%), Positives = 12/22 (54%)
Frame = +3
Query: 42 TVSDACKTTYEEIKNDKKHRYV 107
TV D C + IK+ KH YV
Sbjct: 316 TVEDFCNNIHSSIKSQFKHAYV 337
>SPBP35G2.03c |sgo1||shugoshin Sgo1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 319
Score = 25.0 bits (52), Expect = 3.0
Identities = 14/61 (22%), Positives = 31/61 (50%)
Frame = +3
Query: 60 KTTYEEIKNDKKHRYVVFYIRDEKQIDVETVGERNAEYEQFLEDLQKGGTGECRYGLFDF 239
K + + ++ + K ++ +D K+ D + + + EY F ++L+ TG+ Y DF
Sbjct: 108 KCSSDFLEANVKSQFTTCENKDSKE-DYQILHNKRLEYVSFNDELKSLETGQPLYCFQDF 166
Query: 240 E 242
+
Sbjct: 167 Q 167
>SPAC6F6.12 |||autophagy associated protein
Atg24|Schizosaccharomyces pombe|chr 1|||Manual
Length = 401
Score = 24.6 bits (51), Expect = 3.9
Identities = 11/30 (36%), Positives = 18/30 (60%)
Frame = +3
Query: 120 RDEKQIDVETVGERNAEYEQFLEDLQKGGT 209
RD+KQ DVE + E +A+ + + GG+
Sbjct: 277 RDQKQQDVEALQEYSAKLSLERDKISSGGS 306
>SPAC343.15 |||tRNA isopentenyltransferase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 434
Score = 24.6 bits (51), Expect = 3.9
Identities = 10/36 (27%), Positives = 21/36 (58%)
Frame = -3
Query: 244 YSKSNRPYLHSPVPPFCRSSRNCSYSALRSPTVSTS 137
Y+KS + ++ S P C + ++ S S++ T +T+
Sbjct: 289 YAKSQKKWIQSRFLPMCLAQQDLSPSSILFSTTNTT 324
>SPAC23C4.05c |||LEA domain protein|Schizosaccharomyces pombe|chr
1|||Manual
Length = 431
Score = 24.2 bits (50), Expect = 5.2
Identities = 9/18 (50%), Positives = 12/18 (66%)
Frame = +3
Query: 45 VSDACKTTYEEIKNDKKH 98
V+D CK+ YE+ KN H
Sbjct: 109 VTDKCKSFYEKEKNHASH 126
>SPAC23D3.14c |aah2||alpha-amylase homolog Aah2|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 581
Score = 24.2 bits (50), Expect = 5.2
Identities = 9/22 (40%), Positives = 14/22 (63%)
Frame = +3
Query: 93 KHRYVVFYIRDEKQIDVETVGE 158
KH + F++R K D+ T+GE
Sbjct: 236 KHAPIEFWLRMSKAADIFTIGE 257
>SPAC12B10.16c |mug157||conserved protein |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 509
Score = 24.2 bits (50), Expect = 5.2
Identities = 8/13 (61%), Positives = 12/13 (92%)
Frame = -2
Query: 176 FVFGVAFANRLDV 138
F+FG+AFAN L++
Sbjct: 10 FLFGIAFANNLNI 22
>SPBC3B9.17 |isa2||iron-sulfur protein Isa2|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 205
Score = 23.8 bits (49), Expect = 6.8
Identities = 11/40 (27%), Positives = 18/40 (45%)
Frame = +3
Query: 87 DKKHRYVVFYIRDEKQIDVETVGERNAEYEQFLEDLQKGG 206
D+K + Y++D + +E + ER E L GG
Sbjct: 90 DEKGIPFILYVKDSAKKQLEKIAERKPEENSVLRVTVDGG 129
>SPAC637.12c |mst1||histone acetyltransferase
Mst1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 463
Score = 23.8 bits (49), Expect = 6.8
Identities = 8/21 (38%), Positives = 13/21 (61%)
Frame = +3
Query: 39 VTVSDACKTTYEEIKNDKKHR 101
+ +SD + YE +KN K+ R
Sbjct: 420 ICISDGIEQQYERLKNKKRRR 440
>SPAC26F1.14c |aif1|SPAC29A4.01c|apoptosis-inducing factor homolog
Aif1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 575
Score = 23.4 bits (48), Expect = 9.0
Identities = 7/14 (50%), Positives = 9/14 (64%)
Frame = -1
Query: 261 PWPGACIQSQTGHI 220
PW GAC + TG +
Sbjct: 82 PWHGACFNAATGDV 95
>SPAC821.06 |spn2||septin Spn2|Schizosaccharomyces pombe|chr
1|||Manual
Length = 331
Score = 23.4 bits (48), Expect = 9.0
Identities = 13/40 (32%), Positives = 22/40 (55%)
Frame = +3
Query: 72 EEIKNDKKHRYVVFYIRDEKQIDVETVGERNAEYEQFLED 191
++I NDK ++ YIRD+ + E N+ E+ L+D
Sbjct: 102 DQINNDKCWEPIIKYIRDQHSSYLRR--ELNSHREKRLQD 139
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,299,603
Number of Sequences: 5004
Number of extensions: 22045
Number of successful extensions: 90
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 88
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 89
length of database: 2,362,478
effective HSP length: 64
effective length of database: 2,042,222
effective search space used: 91899990
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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