Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= br--1679
         (657 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC6B1.06c |ubp14|ucp2|ubiquitin C-terminal hydrolase Ubp14|Sch...    45   8e-06
SPAC23G3.08c |ubp7||ubiquitin C-terminal hydrolase Ubp7|Schizosa...    42   1e-04
SPAC16E8.13 |||ubiquitin-protein ligase E3 |Schizosaccharomyces ...    40   3e-04
SPBC577.07 |ubp10||ubiquitin C-terminal hydrolase Ubp10|Schizosa...    36   0.005
SPBC23G7.12c |rpt6|let1|19S proteasome regulatory subunit Rpt6|S...    28   1.0  
SPAC1002.14 |itt1||ubiquitin-protein ligase E3 |Schizosaccharomy...    27   2.4  
SPBC1E8.03c |||conserved fungal protein|Schizosaccharomyces pomb...    27   3.1  
SPAC6F6.11c |||pyridoxine-pyridoxal-pyridoxamine kinase |Schizos...    26   4.2  
SPCC622.13c |||conserved eukaryotic protein|Schizosaccharomyces ...    26   5.5  
SPAC1565.02c |||GTPase activating protein|Schizosaccharomyces po...    26   5.5  
SPBC56F2.09c |arg5||arginine specific carbamoyl-phosphate syntha...    25   9.6  

>SPBC6B1.06c |ubp14|ucp2|ubiquitin C-terminal hydrolase
           Ubp14|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 775

 Score = 45.2 bits (102), Expect = 8e-06
 Identities = 25/61 (40%), Positives = 34/61 (55%), Gaps = 9/61 (14%)
 Frame = +3

Query: 342 SLWMCLRCGTQLCGRTR------NKHALNHF-NTPHSDCYALAANSTTW--EIYCYNCNN 494
           +LWMCL CG   CGR +      N HAL+H+ +T H     L + S     +IYCY+C+ 
Sbjct: 182 NLWMCLTCGALSCGRKQYGGGGGNGHALSHYDDTGHPLAVKLKSISPDGQADIYCYSCDE 241

Query: 495 E 497
           E
Sbjct: 242 E 242



 Score = 29.1 bits (62), Expect = 0.59
 Identities = 12/29 (41%), Positives = 17/29 (58%)
 Frame = +3

Query: 330 EEDLSLWMCLRCGTQLCGRTRNKHALNHF 416
           +E+  + +CL C    CG T  KH+L HF
Sbjct: 33  DEEGGIDLCLTCFQSGCGETGLKHSLVHF 61


>SPAC23G3.08c |ubp7||ubiquitin C-terminal hydrolase
           Ubp7|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 875

 Score = 41.5 bits (93), Expect = 1e-04
 Identities = 18/63 (28%), Positives = 28/63 (44%)
 Frame = +3

Query: 333 EDLSLWMCLRCGTQLCGRTRNKHALNHFNTPHSDCYALAANSTTWEIYCYNCNNEVTASQ 512
           ED  L +C  CG   C    + H+  HF      C  +  N  T + +CY+C+ ++    
Sbjct: 94  EDYELTLCANCGYLFCCNHESDHSRKHFEKNKKHC--VFVNIITLKCHCYSCDADIVIFD 151

Query: 513 PKN 521
            KN
Sbjct: 152 KKN 154


>SPAC16E8.13 |||ubiquitin-protein ligase E3 |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 547

 Score = 39.9 bits (89), Expect = 3e-04
 Identities = 22/56 (39%), Positives = 30/56 (53%), Gaps = 1/56 (1%)
 Frame = +3

Query: 336 DLSLWMCLRCGTQLCGRTRNKHALNHF-NTPHSDCYALAANSTTWEIYCYNCNNEV 500
           D  LW+CL CG   CGR  + HA  H+ +T H  CYA+     T  ++ Y  +N V
Sbjct: 268 DKDLWICLICGNIGCGRYHDAHAKQHYVDTAH--CYAMELE--TQRVWDYAGDNYV 319


>SPBC577.07 |ubp10||ubiquitin C-terminal hydrolase
           Ubp10|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 502

 Score = 35.9 bits (79), Expect = 0.005
 Identities = 32/109 (29%), Positives = 49/109 (44%), Gaps = 7/109 (6%)
 Frame = +3

Query: 309 EVLDPDFEE-------DLSLWMCLRCGTQLCGRTRNKHALNHFNTPHSDCYALAANSTTW 467
           ++LD DFE+       +LS++ CL CG    GR  + HA  H  T +   +    N +T 
Sbjct: 67  KLLDFDFEKVCSVSLTNLSVYACLVCGRYFQGRGPSSHAYFHALTENHHVF---VNCSTL 123

Query: 468 EIYCYNCNNEVTASQPKNXXXXXXXXXXXXWPVALLNHCPLLSYHLKLK 614
           + Y    + +V +S  ++              V  L+H P LSY L LK
Sbjct: 124 KFYVLPESYQVESSALQDIAYVMRPTFTKL-EVQRLDHTPQLSYDLMLK 171


>SPBC23G7.12c |rpt6|let1|19S proteasome regulatory subunit
           Rpt6|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 403

 Score = 28.3 bits (60), Expect = 1.0
 Identities = 20/63 (31%), Positives = 29/63 (46%), Gaps = 3/63 (4%)
 Frame = +3

Query: 258 IGGFEKECSECKKVAKTEVLDPDFEEDLSLWM---CLRCGTQLCGRTRNKHALNHFNTPH 428
           +GG EK+  E K+V +  V  P+  E L +      L  G    G+T    A+ H    H
Sbjct: 147 VGGLEKQIKEIKEVIELPVKHPELFESLGIPQPKGILLYGPPGTGKTLLARAVAH----H 202

Query: 429 SDC 437
           +DC
Sbjct: 203 TDC 205


>SPAC1002.14 |itt1||ubiquitin-protein ligase E3 |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 435

 Score = 27.1 bits (57), Expect = 2.4
 Identities = 15/35 (42%), Positives = 18/35 (51%), Gaps = 4/35 (11%)
 Frame = +3

Query: 354 CLRCGTQ---LCGR-TRNKHALNHFNTPHSDCYAL 446
           CL CGT    LCG     ++   HFN P S CY +
Sbjct: 386 CL-CGTHFCFLCGAYLMEQNPYKHFNDPVSSCYGM 419


>SPBC1E8.03c |||conserved fungal protein|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 477

 Score = 26.6 bits (56), Expect = 3.1
 Identities = 10/45 (22%), Positives = 22/45 (48%)
 Frame = +2

Query: 32  LIIVVSLKPFYCNIYYYFNICKRDYVHKTQWLKKRDRATLEKMET 166
           L++  ++ P+  N YY  +    + + +  W+ K D  +L+   T
Sbjct: 203 LVMFQAINPYLNNNYYTHDTVDTNALEENSWITKLDMQSLQHFRT 247


>SPAC6F6.11c |||pyridoxine-pyridoxal-pyridoxamine kinase
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 309

 Score = 26.2 bits (55), Expect = 4.2
 Identities = 11/27 (40%), Positives = 18/27 (66%)
 Frame = +1

Query: 211 HVAKAVDLTRLKKAIKSEDLKKNVQSA 291
           H+A++ D T    +IK + LKK+V+ A
Sbjct: 231 HIAESPDCTESLASIKEDKLKKSVEMA 257


>SPCC622.13c |||conserved eukaryotic protein|Schizosaccharomyces
            pombe|chr 3|||Manual
          Length = 1098

 Score = 25.8 bits (54), Expect = 5.5
 Identities = 16/68 (23%), Positives = 30/68 (44%)
 Frame = +1

Query: 316  LIQILKKICPCGCVYXVARNSVAEHAINML*ITSTRLILTAMLSLLIPPPGKSTVTTVIM 495
            L+  L+ +C    ++ VAR   +EH +    +  T   +   LS+++          +I 
Sbjct: 915  LLPRLETLCQRWHLFNVARTYSSEHRLQRAMLKVTSACVANKLSIVVYLKLMGITAPIIR 974

Query: 496  KLLHHSQK 519
             + H SQK
Sbjct: 975  AITHMSQK 982


>SPAC1565.02c |||GTPase activating protein|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 374

 Score = 25.8 bits (54), Expect = 5.5
 Identities = 12/30 (40%), Positives = 15/30 (50%)
 Frame = +2

Query: 44  VSLKPFYCNIYYYFNICKRDYVHKTQWLKK 133
           +SLK +Y   YY     K  YV  T W+ K
Sbjct: 75  LSLKAYYGMDYYLHKNVKAVYVIHTDWMSK 104


>SPBC56F2.09c |arg5||arginine specific carbamoyl-phosphate synthase
           Arg5 |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 415

 Score = 25.0 bits (52), Expect = 9.6
 Identities = 11/49 (22%), Positives = 21/49 (42%), Gaps = 3/49 (6%)
 Frame = +3

Query: 318 DPDFEEDLSLW---MCLRCGTQLCGRTRNKHALNHFNTPHSDCYALAAN 455
           DP +++ + ++   +    G   C +      L HF +PH  C  +  N
Sbjct: 86  DPSYKQQILIFTQPLIGNYGVPDCKKRDENGLLRHFESPHIQCAGVVVN 134


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,374,450
Number of Sequences: 5004
Number of extensions: 43323
Number of successful extensions: 141
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 135
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 139
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 297805304
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)