BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= br--1654 (655 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At4g22220.1 68417.m03214 iron-sulfur cluster assembly complex pr... 149 1e-36 At4g04080.1 68417.m00577 iron-sulfur cluster assembly complex pr... 147 6e-36 At3g01020.1 68416.m00003 iron-sulfur cluster assembly complex pr... 146 1e-35 At2g14920.1 68415.m01697 sulfotransferase family protein similar... 30 1.5 At5g66630.1 68418.m08398 LIM domain-containing protein contains ... 29 3.6 At3g50100.1 68416.m05477 exonuclease family protein contains exo... 28 4.7 At3g61590.1 68416.m06898 F-box family protein contains weak hit ... 27 8.2 >At4g22220.1 68417.m03214 iron-sulfur cluster assembly complex protein, putative similar to iron-sulfur cluster assembly complex ISCU1 (GI:11545705) [Homo sapiens]; nifU protein homolog YPL135w (GI:15619823) [Saccharomyces cerevisiae] PIR2:S69049 Length = 167 Score = 149 bits (362), Expect = 1e-36 Identities = 71/86 (82%), Positives = 79/86 (91%), Gaps = 1/86 (1%) Frame = +1 Query: 256 LVGAPACGDVMKLQIXVDEN-GKIVDAKFKTFGCGSAIASSSLATEWVKGKTVDEALKLK 432 LVGAPACGDVMKLQI VDE G+IVDA+FKTFGCGSAIASSS+ATEWVKGK +++ L +K Sbjct: 57 LVGAPACGDVMKLQIKVDEKTGQIVDARFKTFGCGSAIASSSVATEWVKGKAMEDVLTIK 116 Query: 433 NTDIAKELSLPPVKLHCSMLAEDAIK 510 NT+IAK LSLPPVKLHCSMLAEDAIK Sbjct: 117 NTEIAKHLSLPPVKLHCSMLAEDAIK 142 Score = 54.8 bits (126), Expect = 5e-08 Identities = 22/27 (81%), Positives = 23/27 (85%) Frame = +2 Query: 173 YHANVIDHYENPRNVGSLDKKDKNVGT 253 YH NVIDHY+NPRNVGS DK D NVGT Sbjct: 29 YHENVIDHYDNPRNVGSFDKNDPNVGT 55 >At4g04080.1 68417.m00577 iron-sulfur cluster assembly complex protein, putative similar to ISCU2 (GI:11545707) [Homo sapiens] Length = 171 Score = 147 bits (356), Expect = 6e-36 Identities = 67/85 (78%), Positives = 77/85 (90%) Frame = +1 Query: 256 LVGAPACGDVMKLQIXVDENGKIVDAKFKTFGCGSAIASSSLATEWVKGKTVDEALKLKN 435 LVGAP CGDVMKLQ+ D +G+I+DAKFKTFGCGSAIA+SS+ATEWVKGK+V+E L +KN Sbjct: 56 LVGAPQCGDVMKLQVKFDGSGQIIDAKFKTFGCGSAIAASSVATEWVKGKSVEEVLTIKN 115 Query: 436 TDIAKELSLPPVKLHCSMLAEDAIK 510 + IAK LSLPPVKLHCSMLAEDAIK Sbjct: 116 SQIAKHLSLPPVKLHCSMLAEDAIK 140 Score = 56.4 bits (130), Expect = 2e-08 Identities = 23/36 (63%), Positives = 28/36 (77%) Frame = +2 Query: 146 SPNVILAAPYHANVIDHYENPRNVGSLDKKDKNVGT 253 +P +++ YH NVIDHY+NPRNVGS DK D NVGT Sbjct: 19 TPFPVVSRLYHPNVIDHYDNPRNVGSFDKNDPNVGT 54 >At3g01020.1 68416.m00003 iron-sulfur cluster assembly complex protein, putative similar to ISCU2 (GI:11545707) [Homo sapiens]; similar to NIFU-like protein (GI:15919270) [Cowdria ruminantium] Length = 163 Score = 146 bits (353), Expect = 1e-35 Identities = 64/85 (75%), Positives = 77/85 (90%) Frame = +1 Query: 256 LVGAPACGDVMKLQIXVDENGKIVDAKFKTFGCGSAIASSSLATEWVKGKTVDEALKLKN 435 LVGAPACGD+M LQI VD++G+I+D +FKTFGCGSAIASSS+A+EW+KGKT+DE + +KN Sbjct: 55 LVGAPACGDLMSLQIKVDDSGQIIDTRFKTFGCGSAIASSSVASEWIKGKTLDEVMTIKN 114 Query: 436 TDIAKELSLPPVKLHCSMLAEDAIK 510 +IAK L LPPVKLHCSMLAEDAIK Sbjct: 115 AEIAKHLRLPPVKLHCSMLAEDAIK 139 Score = 51.2 bits (117), Expect = 6e-07 Identities = 20/27 (74%), Positives = 23/27 (85%) Frame = +2 Query: 173 YHANVIDHYENPRNVGSLDKKDKNVGT 253 YH NVIDH+ENPRNVGS ++ D NVGT Sbjct: 27 YHENVIDHFENPRNVGSFNRNDPNVGT 53 >At2g14920.1 68415.m01697 sulfotransferase family protein similar to steroid sulfotransferase from [Brassica napus] GI:3420008, GI:3420004; contains Pfam profile PF00685: Sulfotransferase domain Length = 333 Score = 29.9 bits (64), Expect = 1.5 Identities = 20/54 (37%), Positives = 31/54 (57%) Frame = -1 Query: 169 CRKNHIRTSKAFKTHTSTNSVD*KSHFIYFRKTNSDDKRSYILDDVVIDYTLII 8 C +++R+ + KT TS+ VD KS YFRK D +SY+ ++V +II Sbjct: 273 CSLSNLRSVEINKTRTSSR-VDFKS---YFRKGQVGDWKSYMTPEMVDKIDMII 322 >At5g66630.1 68418.m08398 LIM domain-containing protein contains low similarity to Pfam profile PF00412: LIM domain Length = 702 Score = 28.7 bits (61), Expect = 3.6 Identities = 23/89 (25%), Positives = 38/89 (42%), Gaps = 1/89 (1%) Frame = +1 Query: 262 GAPACGDVMKLQIXVDENGKIVDAKFKTFGCG-SAIASSSLATEWVKGKTVDEALKLKNT 438 G P C +L+ + GK+ D ++ CG SA+ S + + E+L +K Sbjct: 435 GTPKCCSCERLEPRGTKYGKLSDGRWLCLECGKSAMDSDECQPLYFDMRDFFESLNMK-- 492 Query: 439 DIAKELSLPPVKLHCSMLAEDAIKQRYQI 525 I KE L V+ E+ I Y++ Sbjct: 493 -IEKEFPLILVRKELLNKKEEKIDNHYEV 520 >At3g50100.1 68416.m05477 exonuclease family protein contains exonuclease domain, Pfam:PF00929 Length = 406 Score = 28.3 bits (60), Expect = 4.7 Identities = 14/41 (34%), Positives = 26/41 (63%) Frame = -3 Query: 425 FSASSTVFPLTHSVARELEAIAEPHPKVLNLASTIFPFSST 303 F ++ T+ + HS+ R+LE + HPKV++ A +F + +T Sbjct: 213 FLSTGTIL-VGHSLNRDLEVLKIDHPKVIDTA-LVFKYPNT 251 >At3g61590.1 68416.m06898 F-box family protein contains weak hit to Pfam PF00646: F-box domain; stamina pistilloidia (Stp) - Pisum sativum, EMBL:AF004843 Length = 411 Score = 27.5 bits (58), Expect = 8.2 Identities = 16/46 (34%), Positives = 23/46 (50%) Frame = -1 Query: 166 RKNHIRTSKAFKTHTSTNSVD*KSHFIYFRKTNSDDKRSYILDDVV 29 R N I +S+ F + S NSV + YF T +DD Y D ++ Sbjct: 69 RWNEIVSSRRFLCNFSNNSVSQRPW--YFMFTTTDDPSGYAYDPII 112 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 13,287,692 Number of Sequences: 28952 Number of extensions: 259651 Number of successful extensions: 666 Number of sequences better than 10.0: 7 Number of HSP's better than 10.0 without gapping: 653 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 665 length of database: 12,070,560 effective HSP length: 78 effective length of database: 9,812,304 effective search space used: 1363910256 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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