Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= br--1646
(691 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC1F5.02 |||protein disulfide isomerase|Schizosaccharomyces po... 82 6e-17
SPAC17H9.14c |||protein disulfide isomerase|Schizosaccharomyces ... 67 2e-12
SPBC577.08c |txl1|trx3|thioredoxin-like I protein Txl1|Schizosac... 47 3e-06
SPAC13F5.05 |||thioredoxin family protein|Schizosaccharomyces po... 43 5e-05
SPAC959.05c |||protein disulfide isomerase |Schizosaccharomyces ... 42 8e-05
SPBC12D12.07c |trx2||mitochondrial thioredoxin Trx2|Schizosaccha... 38 0.001
SPAC7D4.07c |trx1||cytosolic thioredoxin Trx1 |Schizosaccharomyc... 34 0.022
SPBC3D6.13c |||protein disulfide isomerase |Schizosaccharomyces ... 32 0.090
SPBC16G5.02c |||ribokinase |Schizosaccharomyces pombe|chr 2|||Ma... 31 0.12
SPBP16F5.03c |||phosphatidylinositol kinase |Schizosaccharomyces... 26 4.5
SPAC11E3.07 |vma4||V-type ATPase subunit E|Schizosaccharomyces p... 26 5.9
>SPAC1F5.02 |||protein disulfide isomerase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 492
Score = 82.2 bits (194), Expect = 6e-17
Identities = 39/80 (48%), Positives = 53/80 (66%), Gaps = 1/80 (1%)
Frame = +3
Query: 255 GHCKSLAPEYAKAATKLAEEESPIKLAKVDATQEQDLAESYGVRGYPTLKFFRNGSPI-D 431
GHCK+LAPEY AA +L E+ I L +VD T+E DL Y +RGYPTL F+NG I
Sbjct: 52 GHCKALAPEYESAADEL--EKDGISLVEVDCTEEGDLCSEYSIRGYPTLNVFKNGKQISQ 109
Query: 432 YSGGRQADDIISWLKKKTGP 491
YSG R+ D ++ +++K+ P
Sbjct: 110 YSGPRKHDALVKYMRKQLLP 129
Score = 58.0 bits (134), Expect = 1e-09
Identities = 30/78 (38%), Positives = 50/78 (64%), Gaps = 3/78 (3%)
Frame = +3
Query: 255 GHCKSLAPEYAKAATKLAEEESPIKLAKVDATQEQDLAESYGVRGYPTLKFFRNG---SP 425
GHCK+LAP Y K A + + ++S + +AK+DAT E D+ S + G+PT+ FF+ +P
Sbjct: 386 GHCKNLAPTYEKLAEEYS-DDSNVVVAKIDAT-ENDI--SVSISGFPTIMFFKANDKVNP 441
Query: 426 IDYSGGRQADDIISWLKK 479
+ Y G R +D+ +++ K
Sbjct: 442 VRYEGDRTLEDLSAFIDK 459
Score = 33.9 bits (74), Expect = 0.022
Identities = 14/32 (43%), Positives = 24/32 (75%), Gaps = 1/32 (3%)
Frame = +1
Query: 160 TEENVLVLSKANFETVITT-TEYILVEFYAPW 252
++E+++VL NF+ ++ T+ +LVEFYAPW
Sbjct: 353 SQEDLVVLVADNFDDIVMDETKDVLVEFYAPW 384
Score = 27.5 bits (58), Expect = 1.9
Identities = 9/24 (37%), Positives = 16/24 (66%)
Frame = +1
Query: 181 LSKANFETVITTTEYILVEFYAPW 252
++K +IT + ++V+FYAPW
Sbjct: 27 VNKEGLNELITADKVLMVKFYAPW 50
>SPAC17H9.14c |||protein disulfide isomerase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 359
Score = 67.3 bits (157), Expect = 2e-12
Identities = 34/95 (35%), Positives = 55/95 (57%), Gaps = 2/95 (2%)
Frame = +3
Query: 255 GHCKSLAPEYAKAATKLAEEESPIKLAKVDATQEQDLAESYGVRGYPTLKFF-RNGS-PI 428
GHCKSLAP Y + L E+ + + + K+DA D+A+ Y + G+PTL +F +GS P+
Sbjct: 52 GHCKSLAPVYEELGA-LFEDHNDVLIGKIDADTHSDVADKYHITGFPTLIWFPPDGSEPV 110
Query: 429 DYSGGRQADDIISWLKKKTGPPAVEVTLLNRLKNL 533
YS R D + ++ +KTG ++ L + + L
Sbjct: 111 QYSNARDVDSLTQFVSEKTGIKKRKIVLPSNVVEL 145
Score = 51.6 bits (118), Expect = 1e-07
Identities = 26/81 (32%), Positives = 44/81 (54%), Gaps = 3/81 (3%)
Frame = +3
Query: 255 GHCKSLAPEYAKAATKLAEEESPIKLAKVDATQEQDLAESYGVRGYPTLKFF---RNGSP 425
G+CK LAP Y + K+ + E +++ K++A D+ + V +PT+KFF P
Sbjct: 171 GYCKRLAPTY-ETLGKVFKNEPNVEIVKINADVFADIGRLHEVASFPTIKFFPKDDKDKP 229
Query: 426 IDYSGGRQADDIISWLKKKTG 488
Y G R + +I ++ KK+G
Sbjct: 230 ELYEGDRSLESLIEYINKKSG 250
Score = 28.3 bits (60), Expect = 1.1
Identities = 13/29 (44%), Positives = 18/29 (62%), Gaps = 1/29 (3%)
Frame = +1
Query: 169 NVLVLSKANFETVITTTEY-ILVEFYAPW 252
NV+ L NF+ V+ + +LVEFYA W
Sbjct: 141 NVVELDSLNFDKVVMDDKKDVLVEFYADW 169
>SPBC577.08c |txl1|trx3|thioredoxin-like I protein
Txl1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 290
Score = 46.8 bits (106), Expect = 3e-06
Identities = 22/63 (34%), Positives = 36/63 (57%)
Frame = +3
Query: 255 GHCKSLAPEYAKAATKLAEEESPIKLAKVDATQEQDLAESYGVRGYPTLKFFRNGSPIDY 434
G CK+++P +++ A+K A + AKV+ +++ +A GV+ PT FF NG ID
Sbjct: 32 GPCKAISPLFSQLASKYASPK--FVFAKVNVDEQRQIASGLGVKAMPTFVFFENGKQIDM 89
Query: 435 SGG 443
G
Sbjct: 90 LTG 92
>SPAC13F5.05 |||thioredoxin family protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 363
Score = 42.7 bits (96), Expect = 5e-05
Identities = 25/70 (35%), Positives = 35/70 (50%), Gaps = 6/70 (8%)
Frame = +3
Query: 255 GHCKSLAPEYAKAATKLAEEESPIKLAKVDATQEQDLAESYGVRGYPTLKFF---RNGSP 425
G+CK L P Y K A+ L P+ DA Q + + Y V+G+PT+K GS
Sbjct: 61 GYCKKLVPTYQKLASNL-HSLLPVTAVDCDADQNRAVCSQYQVQGFPTIKLVYPSSKGSS 119
Query: 426 I---DYSGGR 446
+ DY+G R
Sbjct: 120 LSSTDYNGDR 129
Score = 26.6 bits (56), Expect = 3.4
Identities = 14/49 (28%), Positives = 19/49 (38%)
Frame = +1
Query: 106 LIFTAIALLGLALGDEVPTEENVLVLSKANFETVITTTEYILVEFYAPW 252
L +L+ G N + L+ NF + LV FYAPW
Sbjct: 11 LFLACFSLVSGVFGYSPMFGSNTIELNSKNFRKFVKAKGPSLVVFYAPW 59
>SPAC959.05c |||protein disulfide isomerase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 632
Score = 41.9 bits (94), Expect = 8e-05
Identities = 22/111 (19%), Positives = 55/111 (49%), Gaps = 2/111 (1%)
Frame = +3
Query: 258 HCKSLAPEYAKAATKLAEEESPIKLAKVDATQEQDLAESYGVRGYPTLKFFRNGSPIDYS 437
HC+ A ++ + +K+A+V+ +E+++ + ++ +PT + F+ I Y+
Sbjct: 208 HCEDCFHWEAVWSSITRNTDERLKMAQVNCDEEKEMCNHFHIKKFPTFRVFQGFDSIQYN 267
Query: 438 GGRQADDIISWLKKKTGPPAVEVTL--LNRLKNLSMPILLLYLVSFRTRAQ 584
G + ++S+ + A+++ + ++N S P+ L L F T ++
Sbjct: 268 GPLKYQQLLSYSNQVASYQAIKIEEGDIESIEN-SHPVFFLVLYDFATTSE 317
Score = 28.7 bits (61), Expect = 0.84
Identities = 22/71 (30%), Positives = 32/71 (45%), Gaps = 7/71 (9%)
Frame = +3
Query: 363 LAESYGVRGYPTLKFFRNGSPIDYSG--GRQADD---IISWLKKKTGPPAVEV--TLLNR 521
LA YG + P++ RNG PI Y R+ D I W+ + P E+ T +
Sbjct: 343 LANKYGAQSQPSIIAVRNGMPIVYQAITPREFRDYKRITEWINIVSSPFITELTPTKCHS 402
Query: 522 LKNLSMPILLL 554
L N + +L L
Sbjct: 403 LLNRKLTVLTL 413
>SPBC12D12.07c |trx2||mitochondrial thioredoxin
Trx2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 121
Score = 38.3 bits (85), Expect = 0.001
Identities = 24/75 (32%), Positives = 33/75 (44%)
Frame = +3
Query: 255 GHCKSLAPEYAKAATKLAEEESPIKLAKVDATQEQDLAESYGVRGYPTLKFFRNGSPIDY 434
G CK L P KL+E+ V+A + D+A+ GV PT+ FR G +D
Sbjct: 48 GPCKYLKP----FLEKLSEQNQKASFIAVNADKFSDIAQKNGVYALPTMVLFRKGQELDR 103
Query: 435 SGGRQADDIISWLKK 479
G + S L K
Sbjct: 104 IVGADVKTLSSLLAK 118
>SPAC7D4.07c |trx1||cytosolic thioredoxin Trx1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 103
Score = 33.9 bits (74), Expect = 0.022
Identities = 17/59 (28%), Positives = 29/59 (49%)
Frame = +3
Query: 255 GHCKSLAPEYAKAATKLAEEESPIKLAKVDATQEQDLAESYGVRGYPTLKFFRNGSPID 431
G CK++AP++ + + S KVD Q ++A GV P+ ++NG I+
Sbjct: 31 GPCKAIAPKFEQ----FSNTYSDATFIKVDVDQLSEIAAEAGVHAMPSFFLYKNGEKIE 85
>SPBC3D6.13c |||protein disulfide isomerase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 726
Score = 31.9 bits (69), Expect = 0.090
Identities = 19/61 (31%), Positives = 29/61 (47%), Gaps = 2/61 (3%)
Frame = +3
Query: 255 GHCKSLAPEYAKAATKLAE--EESPIKLAKVDATQEQDLAESYGVRGYPTLKFFRNGSPI 428
G CK L P + K E E S +VD ++E L+ +R PTL ++NG +
Sbjct: 55 GACKRLGPMWDNMVEKAKEQVEGSNFHFGEVDCSKE--LSSCANIRAVPTLYLYQNGEIV 112
Query: 429 D 431
+
Sbjct: 113 E 113
Score = 31.9 bits (69), Expect = 0.090
Identities = 14/65 (21%), Positives = 33/65 (50%)
Frame = +3
Query: 324 IKLAKVDATQEQDLAESYGVRGYPTLKFFRNGSPIDYSGGRQADDIISWLKKKTGPPAVE 503
+ +A ++ + + Y ++ +PT FF+ + ++Y G D++S+ ++ E
Sbjct: 331 LNVAHINCAVSKRACKQYSIQYFPTFLFFKEEAFVEYVGLPNEGDLVSFAEEAANFEIRE 390
Query: 504 VTLLN 518
V LL+
Sbjct: 391 VELLD 395
>SPBC16G5.02c |||ribokinase |Schizosaccharomyces pombe|chr
2|||Manual
Length = 318
Score = 31.5 bits (68), Expect = 0.12
Identities = 21/68 (30%), Positives = 31/68 (45%)
Frame = +3
Query: 249 MVGHCKSLAPEYAKAATKLAEEESPIKLAKVDATQEQDLAESYGVRGYPTLKFFRNGSPI 428
M+ +C L P +AA L + +SP L VDA + L S+GVR + G+
Sbjct: 180 MISYCAYLVPNEHEAAILLNQADSPATLENVDAYASKLL--SFGVRKAVIITLGSQGAYY 237
Query: 429 DYSGGRQA 452
+ G A
Sbjct: 238 KSANGESA 245
>SPBP16F5.03c |||phosphatidylinositol kinase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 3699
Score = 26.2 bits (55), Expect = 4.5
Identities = 12/31 (38%), Positives = 17/31 (54%)
Frame = -1
Query: 349 VASTFASLIGDSSSASFVAALAYSGARDLQW 257
V++ F SL+G S FVA + R L+W
Sbjct: 1039 VSNIFKSLVGSMSCVEFVAEARETINRSLEW 1069
>SPAC11E3.07 |vma4||V-type ATPase subunit E|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 227
Score = 25.8 bits (54), Expect = 5.9
Identities = 15/46 (32%), Positives = 24/46 (52%)
Frame = +3
Query: 249 MVGHCKSLAPEYAKAATKLAEEESPIKLAKVDATQEQDLAESYGVR 386
MV K A E AK L+EEE ++ AK+ Q + ++Y ++
Sbjct: 15 MVSFIKQEALEKAKEIHTLSEEEFQVEKAKIVREQCDAIDQTYDMK 60
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,689,431
Number of Sequences: 5004
Number of extensions: 51737
Number of successful extensions: 183
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 163
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 177
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 319939482
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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