BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= br--1644
(750 letters)
Database: mosquito
2352 sequences; 563,979 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
AY255856-1|AAP13482.1| 248|Anopheles gambiae glutathione transf... 91 4e-20
AF515522-1|AAM61889.1| 222|Anopheles gambiae glutathione S-tran... 39 1e-04
AY070234-1|AAL58538.1| 223|Anopheles gambiae glutathione S-tran... 26 1.1
>AY255856-1|AAP13482.1| 248|Anopheles gambiae glutathione
transferase o1 protein.
Length = 248
Score = 90.6 bits (215), Expect = 4e-20
Identities = 45/84 (53%), Positives = 58/84 (69%), Gaps = 4/84 (4%)
Frame = +3
Query: 273 NTKHLRKGDPLP--PFNGKLRVYNMRYCPYAQRTILALNAKQIDYEVVNIDLIDKPEWLT 446
N KHL KG P P +GKLR+Y+MR+CPYAQR L L+AK+I Y + I+L +KPEW
Sbjct: 3 NGKHLAKGSSPPSLPDDGKLRLYSMRFCPYAQRVHLMLDAKKIPYHAIYINLSEKPEWYL 62
Query: 447 TKSAFAKVPAIEI--AEDVTIYEA 512
K+ KVPA+EI E VT+YE+
Sbjct: 63 EKNPLGKVPALEIPGKEGVTLYES 86
Score = 42.7 bits (96), Expect = 1e-05
Identities = 24/75 (32%), Positives = 41/75 (54%), Gaps = 3/75 (4%)
Frame = +2
Query: 509 SLVTVEYLDEVYP--KRPLLPQDPLKKALDKIIVEASA-PIQSLFIKILKFSDTVNEEHV 679
SLV +Y++E Y +R L P DP KA D+I++E A + + +IL +D + +
Sbjct: 86 SLVLSDYIEEAYSAQQRKLYPADPFSKAQDRILIERFAGSVIGPYYRILFAADGIPPGAI 145
Query: 680 AAYHKALDFIQEQLK 724
+ LD +++LK
Sbjct: 146 TEFGAGLDIFEKELK 160
>AF515522-1|AAM61889.1| 222|Anopheles gambiae glutathione
S-transferase protein.
Length = 222
Score = 39.1 bits (87), Expect = 1e-04
Identities = 19/51 (37%), Positives = 32/51 (62%), Gaps = 4/51 (7%)
Frame = +2
Query: 503 LRSLVTVEYLDEVYPKRPLLPQDPLKKA----LDKIIVEASAPIQSLFIKI 643
+ S+ + YL+E P+RPL+PQD LK+A + ++I P+Q+L + I
Sbjct: 76 IESVSIMYYLEETRPQRPLMPQDVLKRAKVREICEVIASGVQPLQNLIVLI 126
Score = 23.8 bits (49), Expect = 5.8
Identities = 16/43 (37%), Positives = 21/43 (48%)
Frame = +3
Query: 297 DPLPPFNGKLRVYNMRYCPYAQRTILALNAKQIDYEVVNIDLI 425
D LP L Y C + R +ALN K+I Y++ I LI
Sbjct: 5 DILPESQPILYSYWRSSCSWRVR--IALNLKEIPYDIKPISLI 45
>AY070234-1|AAL58538.1| 223|Anopheles gambiae glutathione
S-transferase E3 protein.
Length = 223
Score = 26.2 bits (55), Expect = 1.1
Identities = 17/66 (25%), Positives = 31/66 (46%), Gaps = 1/66 (1%)
Frame = +2
Query: 533 DEVYPKRPLLPQDPLKKALDKIIVEASAPIQSLFIKILKFSDT-VNEEHVAAYHKALDFI 709
D +YP + L+ Q + L A ++ + + + T V +E + + HKA D +
Sbjct: 83 DTLYPAKDLVKQANINALLHFESGVLFARLRWILEPVFYWGQTEVPQEKIDSVHKAYDLL 142
Query: 710 QEQLKT 727
+ LKT
Sbjct: 143 EATLKT 148
Database: mosquito
Posted date: Oct 23, 2007 1:18 PM
Number of letters in database: 563,979
Number of sequences in database: 2352
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 743,036
Number of Sequences: 2352
Number of extensions: 15167
Number of successful extensions: 23
Number of sequences better than 10.0: 3
Number of HSP's better than 10.0 without gapping: 21
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 23
length of database: 563,979
effective HSP length: 63
effective length of database: 415,803
effective search space used: 77339358
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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