BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= br--1644 (750 letters) Database: mosquito 2352 sequences; 563,979 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value AY255856-1|AAP13482.1| 248|Anopheles gambiae glutathione transf... 91 4e-20 AF515522-1|AAM61889.1| 222|Anopheles gambiae glutathione S-tran... 39 1e-04 AY070234-1|AAL58538.1| 223|Anopheles gambiae glutathione S-tran... 26 1.1 >AY255856-1|AAP13482.1| 248|Anopheles gambiae glutathione transferase o1 protein. Length = 248 Score = 90.6 bits (215), Expect = 4e-20 Identities = 45/84 (53%), Positives = 58/84 (69%), Gaps = 4/84 (4%) Frame = +3 Query: 273 NTKHLRKGDPLP--PFNGKLRVYNMRYCPYAQRTILALNAKQIDYEVVNIDLIDKPEWLT 446 N KHL KG P P +GKLR+Y+MR+CPYAQR L L+AK+I Y + I+L +KPEW Sbjct: 3 NGKHLAKGSSPPSLPDDGKLRLYSMRFCPYAQRVHLMLDAKKIPYHAIYINLSEKPEWYL 62 Query: 447 TKSAFAKVPAIEI--AEDVTIYEA 512 K+ KVPA+EI E VT+YE+ Sbjct: 63 EKNPLGKVPALEIPGKEGVTLYES 86 Score = 42.7 bits (96), Expect = 1e-05 Identities = 24/75 (32%), Positives = 41/75 (54%), Gaps = 3/75 (4%) Frame = +2 Query: 509 SLVTVEYLDEVYP--KRPLLPQDPLKKALDKIIVEASA-PIQSLFIKILKFSDTVNEEHV 679 SLV +Y++E Y +R L P DP KA D+I++E A + + +IL +D + + Sbjct: 86 SLVLSDYIEEAYSAQQRKLYPADPFSKAQDRILIERFAGSVIGPYYRILFAADGIPPGAI 145 Query: 680 AAYHKALDFIQEQLK 724 + LD +++LK Sbjct: 146 TEFGAGLDIFEKELK 160 >AF515522-1|AAM61889.1| 222|Anopheles gambiae glutathione S-transferase protein. Length = 222 Score = 39.1 bits (87), Expect = 1e-04 Identities = 19/51 (37%), Positives = 32/51 (62%), Gaps = 4/51 (7%) Frame = +2 Query: 503 LRSLVTVEYLDEVYPKRPLLPQDPLKKA----LDKIIVEASAPIQSLFIKI 643 + S+ + YL+E P+RPL+PQD LK+A + ++I P+Q+L + I Sbjct: 76 IESVSIMYYLEETRPQRPLMPQDVLKRAKVREICEVIASGVQPLQNLIVLI 126 Score = 23.8 bits (49), Expect = 5.8 Identities = 16/43 (37%), Positives = 21/43 (48%) Frame = +3 Query: 297 DPLPPFNGKLRVYNMRYCPYAQRTILALNAKQIDYEVVNIDLI 425 D LP L Y C + R +ALN K+I Y++ I LI Sbjct: 5 DILPESQPILYSYWRSSCSWRVR--IALNLKEIPYDIKPISLI 45 >AY070234-1|AAL58538.1| 223|Anopheles gambiae glutathione S-transferase E3 protein. Length = 223 Score = 26.2 bits (55), Expect = 1.1 Identities = 17/66 (25%), Positives = 31/66 (46%), Gaps = 1/66 (1%) Frame = +2 Query: 533 DEVYPKRPLLPQDPLKKALDKIIVEASAPIQSLFIKILKFSDT-VNEEHVAAYHKALDFI 709 D +YP + L+ Q + L A ++ + + + T V +E + + HKA D + Sbjct: 83 DTLYPAKDLVKQANINALLHFESGVLFARLRWILEPVFYWGQTEVPQEKIDSVHKAYDLL 142 Query: 710 QEQLKT 727 + LKT Sbjct: 143 EATLKT 148 Database: mosquito Posted date: Oct 23, 2007 1:18 PM Number of letters in database: 563,979 Number of sequences in database: 2352 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 743,036 Number of Sequences: 2352 Number of extensions: 15167 Number of successful extensions: 23 Number of sequences better than 10.0: 3 Number of HSP's better than 10.0 without gapping: 21 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 23 length of database: 563,979 effective HSP length: 63 effective length of database: 415,803 effective search space used: 77339358 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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