Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= br--1642
(662 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At4g29010.1 68417.m04147 abnormal inflorescence meristem 1 / fat... 55 4e-08
At3g06860.1 68416.m00814 fatty acid multifunctional protein (MFP... 54 1e-07
At4g16800.1 68417.m02537 enoyl-CoA hydratase, putative similar t... 50 1e-06
At5g43280.1 68418.m05290 enoyl-CoA hydratase/isomerase family pr... 37 0.014
At2g41750.1 68415.m05161 DTW domain-containing protein contains ... 32 0.30
At3g19320.1 68416.m02450 leucine-rich repeat family protein cont... 32 0.39
At5g65940.1 68418.m08301 3-hydroxyisobutyryl-coenzyme A hydrolas... 31 0.68
At4g36420.1 68417.m05174 ribosomal protein L12 family protein 30 1.2
At2g30650.1 68415.m03738 3-hydroxyisobutyryl-coenzyme A hydrolas... 30 1.6
At3g29810.1 68416.m03794 phytochelatin synthetase family protein... 29 2.8
At2g30660.1 68415.m03739 3-hydroxyisobutyryl-coenzyme A hydrolas... 29 2.8
At4g33420.1 68417.m04749 peroxidase, putative identical to class... 29 3.6
At4g08113.1 68417.m01331 myosin heavy chain-related similar to M... 29 3.6
At2g19930.1 68415.m02329 RNA-dependent RNA polymerase family pro... 29 3.6
At4g08780.1 68417.m01447 peroxidase, putative similar to peroxid... 28 4.8
At2g39280.1 68415.m04823 RabGAP/TBC domain-containing protein co... 28 6.4
At2g15880.1 68415.m01820 leucine-rich repeat family protein / ex... 27 8.4
At1g60190.1 68414.m06780 armadillo/beta-catenin repeat family pr... 27 8.4
>At4g29010.1 68417.m04147 abnormal inflorescence meristem 1 / fatty
acid multifunctional protein (AIM1) identical to
gi:4337025; contains Pfam profiles PF02737
(3-hydroxyacyl-CoA dehydrogenase, NAD binding domain),
PF00378 (enoyl-CoA hydratase/isomerase family protein),
PF00725 (3-hydroxyacyl-CoA dehydrogenase)
Length = 721
Score = 55.2 bits (127), Expect = 4e-08
Identities = 27/49 (55%), Positives = 33/49 (67%)
Frame = +3
Query: 516 LGGGLETALACKYRIAVKDSKTGFGLPEVMLGLLPGGGRTQRLPALTSI 662
LGGGLE A+AC R+A K GLPE+ LG++PG G TQRLP L +
Sbjct: 111 LGGGLELAMACHARVAAP--KAQLGLPELTLGVIPGFGGTQRLPRLVGL 157
Score = 37.5 bits (83), Expect = 0.008
Identities = 23/88 (26%), Positives = 45/88 (51%), Gaps = 1/88 (1%)
Frame = +1
Query: 247 LSNVKVNSLNTQVMEEVSNIVNEIETNSGIEAAVIISGKPGCFIAGADISMIENCKTKEE 426
+SN VNSL + ++ + + + ++A V+I G G F G DI++ + +
Sbjct: 21 ISNPPVNSLASPIISGLKEKFRDANQRNDVKAIVLI-GNNGRFSGGFDINVFQQVHKTGD 79
Query: 427 VVSLSKRGHE-IFRRIEQSRKPYIAAIQ 507
+ + + E + +E SRKP +AA++
Sbjct: 80 LSLMPEVSVELVCNLMEDSRKPVVAAVE 107
>At3g06860.1 68416.m00814 fatty acid multifunctional protein (MFP2)
identical to fatty acid multifunctional protein (AtMFP2)
GB:AF123254 [gi:4337027] (Arabidopsis thaliana) (fatty
acid beta-oxidation); contains Pfam profiles PF02737
(3-hydroxyacyl-CoA dehydrogenase, NAD binding domain),
PF00378 (enoyl-CoA hydratase/isomerase family protein),
PF00725 (3-hydroxyacyl-CoA dehydrogenase)
Length = 725
Score = 53.6 bits (123), Expect = 1e-07
Identities = 26/49 (53%), Positives = 34/49 (69%)
Frame = +3
Query: 516 LGGGLETALACKYRIAVKDSKTGFGLPEVMLGLLPGGGRTQRLPALTSI 662
LGGGLE A+AC RI+ ++ GLPE+ LG++PG G TQRLP L +
Sbjct: 114 LGGGLELAMACHARISAPAAQ--LGLPELQLGVIPGFGGTQRLPRLVGL 160
>At4g16800.1 68417.m02537 enoyl-CoA hydratase, putative similar to
AU-binding protein/Enoyl-CoA hydratase [Homo sapiens]
GI:780241, [Mus musculus]GI:6840920; contains Pfam
profile PF00378 enoyl-CoA hydratase/isomerase family
protein
Length = 229
Score = 50.4 bits (115), Expect = 1e-06
Identities = 26/48 (54%), Positives = 32/48 (66%)
Frame = +3
Query: 510 SCLGGGLETALACKYRIAVKDSKTGFGLPEVMLGLLPGGGRTQRLPAL 653
+ LGGGLE ALAC RI +++ FGLPE L ++PG G TQRL L
Sbjct: 76 AALGGGLEMALACDLRICGENAV--FGLPETGLAIIPGAGGTQRLSRL 121
Score = 30.7 bits (66), Expect = 0.90
Identities = 22/77 (28%), Positives = 34/77 (44%)
Frame = +1
Query: 283 VMEEVSNIVNEIETNSGIEAAVIISGKPGCFIAGADISMIENCKTKEEVVSLSKRGHEIF 462
+++ + N I ++ +I S PG F AGAD+ + EV + +F
Sbjct: 1 MLKSLQNAFESIHQDNSARVVMIRSLVPGVFCAGADLKE-RRTMSPSEVHTYVNSLRYMF 59
Query: 463 RRIEQSRKPYIAAIQAA 513
IE P IAAI+ A
Sbjct: 60 SFIEALSIPTIAAIEGA 76
>At5g43280.1 68418.m05290 enoyl-CoA hydratase/isomerase family
protein similar to Delta 3,5-delta2,4-dienoyl-CoA
isomerase, mitochondrial (ECH1) from Rattus norvegicus
[SP|Q62651], from Homo sapiens [SP|Q13011]; contains
Pfam profile PF00378 enoyl-CoA hydratase/isomerase
family protein
Length = 278
Score = 36.7 bits (81), Expect = 0.014
Identities = 25/86 (29%), Positives = 44/86 (51%), Gaps = 6/86 (6%)
Frame = +3
Query: 414 NKGRSCQSV*KRT*NIQKNRTITETVYCRNT------SSCLGGGLETALACKYRIAVKDS 575
++GRS + + ++ ++Q IT CR +C+GGG++ AC R +D+
Sbjct: 88 DRGRSSEQLRRKIKSMQA--AITAIEQCRKPVIAAIHGACIGGGVDLITACDIRYCSEDA 145
Query: 576 KTGFGLPEVMLGLLPGGGRTQRLPAL 653
F + EV L ++ G QRLP++
Sbjct: 146 --FFSIKEVDLAIVADLGTLQRLPSI 169
>At2g41750.1 68415.m05161 DTW domain-containing protein contains
Pfam domain, PF03942: DTW domain
Length = 253
Score = 32.3 bits (70), Expect = 0.30
Identities = 19/51 (37%), Positives = 27/51 (52%), Gaps = 2/51 (3%)
Frame = -2
Query: 649 AGNRCVRPPPG--KSPSMTSGSPNPVLESFTAMRYLHARAVSNPPPRQLLV 503
AG + PP PS S SP + F ++ L+ R +SNPPP +L+V
Sbjct: 82 AGGTALPAPPTIYLFPSSPS-SPAVTISEFKSLNLLNHREISNPPPLRLIV 131
>At3g19320.1 68416.m02450 leucine-rich repeat family protein
contains leucine-rich repeats, Pfam:PF00560;
Length = 493
Score = 31.9 bits (69), Expect = 0.39
Identities = 16/40 (40%), Positives = 19/40 (47%)
Frame = -2
Query: 628 PPPGKSPSMTSGSPNPVLESFTAMRYLHARAVSNPPPRQL 509
PPP S+ SP+P E + Y H S PPPR L
Sbjct: 70 PPPPPPQSLPPPSPSPEPEHYPPPPYHHYITPSPPPPRPL 109
>At5g65940.1 68418.m08301 3-hydroxyisobutyryl-coenzyme A hydrolase /
CoA-thioester hydrolase (CHY1) identical to gi:8572760;
contains Pfam profile PF00388 enoyl-CoA
hydratase/isomerase family protein
Length = 378
Score = 31.1 bits (67), Expect = 0.68
Identities = 17/46 (36%), Positives = 26/46 (56%)
Frame = +3
Query: 516 LGGGLETALACKYRIAVKDSKTGFGLPEVMLGLLPGGGRTQRLPAL 653
+GGG ++ ++RIA ++ T F +PE LGL P G + L L
Sbjct: 117 MGGGAGVSVHGRFRIATEN--TVFAMPETALGLFPDVGASYFLSRL 160
>At4g36420.1 68417.m05174 ribosomal protein L12 family protein
Length = 179
Score = 30.3 bits (65), Expect = 1.2
Identities = 18/57 (31%), Positives = 34/57 (59%), Gaps = 2/57 (3%)
Frame = +2
Query: 80 NLKVMAN--SKILSALKILRSRKELFISGVHSRKYAVPASQVHTKCKLVNGVYVVTL 244
NL+++++ S++L + + S +LF + SR Y+ PA+Q K+VN + +TL
Sbjct: 3 NLRIISSHFSRVLKSTETRSSSVQLFT--IQSRSYSSPATQSENVSKIVNELSNLTL 57
>At2g30650.1 68415.m03738 3-hydroxyisobutyryl-coenzyme A hydrolase,
putative / CoA-thioester hydrolase, putative strong
similarity to gi:8572760; contains Pfam profile PF00388
enoyl-CoA hydratase/isomerase family protein
Length = 422
Score = 29.9 bits (64), Expect = 1.6
Identities = 17/46 (36%), Positives = 25/46 (54%)
Frame = +3
Query: 516 LGGGLETALACKYRIAVKDSKTGFGLPEVMLGLLPGGGRTQRLPAL 653
+GGG + ++RIA ++ T F +PE LGL P G + L L
Sbjct: 157 MGGGAGLSTNGRFRIATEN--TVFAMPETALGLFPDVGASYFLSRL 200
>At3g29810.1 68416.m03794 phytochelatin synthetase family protein /
COBRA cell expansion protein COBL2 similar to
phytochelatin synthetase [Hordeum vulgare subsp.
vulgare] GI:29570314; identified in Roudier, et al,
Plant Phys. (2002) 130:538-548 (PMID:12376623); contains
Pfam profile PF04833: Phytochelatin synthetase-like
conserved region
Length = 441
Score = 29.1 bits (62), Expect = 2.8
Identities = 11/30 (36%), Positives = 15/30 (50%)
Frame = -2
Query: 646 GNRCVRPPPGKSPSMTSGSPNPVLESFTAM 557
G+ CV PPP P + + SPN F +
Sbjct: 401 GDNCVMPPPDSYPWLPNASPNIATSPFVIL 430
>At2g30660.1 68415.m03739 3-hydroxyisobutyryl-coenzyme A hydrolase,
putative / CoA-thioester hydrolase, putative strong
similarity to gi:8572760; contains Pfam profile PF00388
enoyl-CoA hydratase/isomerase family protein
Length = 378
Score = 29.1 bits (62), Expect = 2.8
Identities = 16/46 (34%), Positives = 25/46 (54%)
Frame = +3
Query: 516 LGGGLETALACKYRIAVKDSKTGFGLPEVMLGLLPGGGRTQRLPAL 653
+G G ++ ++RIA ++ T F +PE LGL P G + L L
Sbjct: 113 MGAGAGVSIHGRFRIATEN--TVFAMPETSLGLFPDVGASYFLSRL 156
>At4g33420.1 68417.m04749 peroxidase, putative identical to class
III peroxidase ATP32 [Arabidopsis thaliana]
gi|17530547|gb|AAL40837; identical to cDNA class III
peroxidase ATP32 GI:17530546
Length = 325
Score = 28.7 bits (61), Expect = 3.6
Identities = 17/59 (28%), Positives = 31/59 (52%), Gaps = 5/59 (8%)
Frame = +1
Query: 298 SNIVNEIETNSGIEAAVIISGKPGCFIAGADISMI-----ENCKTKEEVVSLSKRGHEI 459
+++ N ++ + + A +I CFI G D S++ +N K+ +LS RG+EI
Sbjct: 55 NSVNNALQADPTLAAGLIRMLFHDCFIEGCDASILLDSTKDNTAEKDSPANLSLRGYEI 113
>At4g08113.1 68417.m01331 myosin heavy chain-related similar to
Myosin heavy chain, skeletal muscle, extraocular
(MyHC-eo) (SP:Q9UKX3) {Homo sapiens}
Length = 764
Score = 28.7 bits (61), Expect = 3.6
Identities = 19/68 (27%), Positives = 35/68 (51%), Gaps = 1/68 (1%)
Frame = +1
Query: 313 EIETNS-GIEAAVIISGKPGCFIAGADISMIENCKTKEEVVSLSKRGHEIFRRIEQSRKP 489
+++TN+ + IS +PG + GA + +T+ E+++L +RG + SR
Sbjct: 173 QVKTNARSDQGTYYISARPGSLVLGARTKEVTLSETELELLTLLQRGQTNWSTF--SRNR 230
Query: 490 YIAAIQAA 513
IAA+ A
Sbjct: 231 IIAALVVA 238
>At2g19930.1 68415.m02329 RNA-dependent RNA polymerase family
protein contains Pfam domain, PF05183: RNA dependent RNA
polymerase
Length = 977
Score = 28.7 bits (61), Expect = 3.6
Identities = 14/35 (40%), Positives = 17/35 (48%)
Frame = -2
Query: 610 PSMTSGSPNPVLESFTAMRYLHARAVSNPPPRQLL 506
PSM S +P L +F+ L SNPP R L
Sbjct: 470 PSMIKVSKDPSLSNFSTFNALEVVTTSNPPKRTKL 504
>At4g08780.1 68417.m01447 peroxidase, putative similar to peroxidase
isozyme [Armoracia rusticana] gi|217932|dbj|BAA14143
Length = 346
Score = 28.3 bits (60), Expect = 4.8
Identities = 18/57 (31%), Positives = 30/57 (52%), Gaps = 1/57 (1%)
Frame = +1
Query: 235 SHFGLSNVKVNSLNTQVMEEVSN-IVNEIETNSGIEAAVIISGKPGCFIAGADISMI 402
SH LS + QV + V+N IVN + ++ I A+++ CF+ G D S++
Sbjct: 20 SHAQLSPSFYDKTCPQVFDIVTNTIVNALRSDPRIAASILRLHFHDCFVNGCDASIL 76
>At2g39280.1 68415.m04823 RabGAP/TBC domain-containing protein
contains Pfam profile PF00566: TBC domain
Length = 771
Score = 27.9 bits (59), Expect = 6.4
Identities = 24/88 (27%), Positives = 43/88 (48%)
Frame = +1
Query: 244 GLSNVKVNSLNTQVMEEVSNIVNEIETNSGIEAAVIISGKPGCFIAGADISMIENCKTKE 423
G+S +S NT V S+ E E N G E + + KP G+D++ N + ++
Sbjct: 57 GVSPTNGSSENTHVNPSESDKKKEKELNKGAERKDLNADKP-----GSDLTP-GNAREED 110
Query: 424 EVVSLSKRGHEIFRRIEQSRKPYIAAIQ 507
EV + K H++ ++ +P + AI+
Sbjct: 111 EVPNREKNVHKV--QLWAEIRPSLQAIE 136
>At2g15880.1 68415.m01820 leucine-rich repeat family protein /
extensin family protein similar to extensin-like protein
[Lycopersicon esculentum] gi|5917664|gb|AAD55979;
contains leucine-rich repeats, Pfam:PF00560; contains
proline rich extensin domains, INTERPRO:IPR002965
Length = 727
Score = 27.5 bits (58), Expect = 8.4
Identities = 14/39 (35%), Positives = 20/39 (51%)
Frame = -2
Query: 634 VRPPPGKSPSMTSGSPNPVLESFTAMRYLHARAVSNPPP 518
V PP ++PS T +P P E F ++ + S PPP
Sbjct: 685 VNSPPPRTPSQTVEAP-PPSEEFIIPPFIGHQYASPPPP 722
>At1g60190.1 68414.m06780 armadillo/beta-catenin repeat family
protein / U-box domain-containing protein contains Pfam
domain, PF00514: Armadillo/beta-catenin-like repeats and
Pfam, PF04564: U-box domain
Length = 686
Score = 27.5 bits (58), Expect = 8.4
Identities = 14/47 (29%), Positives = 26/47 (55%)
Frame = +3
Query: 456 NIQKNRTITETVYCRNTSSCLGGGLETALACKYRIAVKDSKTGFGLP 596
++ KN +I ++Y +++ LGGG + + K ++ KTG G P
Sbjct: 629 SLAKNPSIMGSLYTASSNGELGGGKKASALIKMIHEFQERKTGPGEP 675
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 13,446,638
Number of Sequences: 28952
Number of extensions: 283090
Number of successful extensions: 947
Number of sequences better than 10.0: 18
Number of HSP's better than 10.0 without gapping: 902
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 942
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1393347168
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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