BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= br--1609
(838 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC23A1.12c |||phenylalanine-tRNA ligase beta subunit |Schizosa... 28 1.9
SPBP4H10.07 |||ubiquitin-protein ligase E3 |Schizosaccharomyces ... 27 4.4
SPAC4H3.07c |||protein phosphatase Fmp31 |Schizosaccharomyces po... 26 7.6
>SPAC23A1.12c |||phenylalanine-tRNA ligase beta subunit
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 589
Score = 27.9 bits (59), Expect = 1.9
Identities = 15/51 (29%), Positives = 25/51 (49%)
Frame = +3
Query: 348 TLFRFYLLLPFHISLINVIKY*YALTQLLENSYTSSANASLDFMNIASFIS 500
T+F Y PF I +N+I T++ N + A +D++N A +S
Sbjct: 259 TMFSCYCEEPFTIEPVNIISEHNGCTRVTPNLNPTCFKADIDYLNEACGLS 309
>SPBP4H10.07 |||ubiquitin-protein ligase E3 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 583
Score = 26.6 bits (56), Expect = 4.4
Identities = 20/66 (30%), Positives = 29/66 (43%), Gaps = 2/66 (3%)
Frame = -1
Query: 265 DYVLPTF--IYFPTFFLPQVSTISRKVLRSNRQPGDDSAILIYGILGLLKSTLLSFHYQE 92
D+ LP IY F+PQ + R P + +L+ I+G+ K + S E
Sbjct: 451 DHQLPRSWAIYVREAFVPQNHPVLRAPSLFTDSPTYEDMLLLNSIIGIEKPPVASQKDLE 510
Query: 91 KEQGVF 74
K GVF
Sbjct: 511 KAGGVF 516
>SPAC4H3.07c |||protein phosphatase Fmp31 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 171
Score = 25.8 bits (54), Expect = 7.6
Identities = 9/25 (36%), Positives = 14/25 (56%)
Frame = -1
Query: 541 VCNVLWTSIKFTLELMKLAIFIKSK 467
+C+V W + FTL L ++ SK
Sbjct: 44 ICDVFWLKLSFTLRYSTLQVYNLSK 68
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,011,034
Number of Sequences: 5004
Number of extensions: 59220
Number of successful extensions: 146
Number of sequences better than 10.0: 3
Number of HSP's better than 10.0 without gapping: 143
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 146
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 412451140
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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