Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= br--1608
(840 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At2g45790.1 68415.m05695 eukaryotic phosphomannomutase family pr... 99 4e-21
At2g23180.1 68415.m02769 cytochrome P450, putative 30 2.2
At4g12430.1 68417.m01967 trehalose-6-phosphate phosphatase, puta... 29 3.8
At2g25870.1 68415.m03105 haloacid dehalogenase-like hydrolase fa... 29 3.8
At1g17665.1 68414.m02187 expressed protein 29 3.8
At5g50090.2 68418.m06203 expressed protein 29 5.1
At5g50090.1 68418.m06202 expressed protein 29 5.1
At4g22590.1 68417.m03259 trehalose-6-phosphate phosphatase, puta... 29 5.1
At1g74930.1 68414.m08693 AP2 domain-containing transcription fac... 29 5.1
At4g27595.1 68417.m03964 protein transport protein-related low s... 28 6.7
At5g17680.1 68418.m02072 disease resistance protein (TIR-NBS-LRR... 28 8.9
At4g18970.1 68417.m02794 GDSL-motif lipase/hydrolase family prot... 28 8.9
>At2g45790.1 68415.m05695 eukaryotic phosphomannomutase family
protein contains Pfam profile: PF03332 eukaryotic
phosphomannomutase
Length = 246
Score = 98.7 bits (235), Expect = 4e-21
Identities = 40/86 (46%), Positives = 62/86 (72%)
Frame = +1
Query: 493 KKIKFKSIVNHLGEQKLQEVINFAMGYMSNIKLPVKRGNFIEFRSSMLNICPVGRSCNQI 672
K I +S+ HLG+ KL+E+INF + Y++++ +P+KRG FIEFR+ MLN+ P+GR+C+Q
Sbjct: 81 KSIGIQSLKLHLGDDKLKELINFTLHYIADLDIPIKRGTFIEFRNGMLNVSPIGRNCSQE 140
Query: 673 ERDQFSEYDSKHKVRHQFVEALQSKF 750
ERD+F YD +R + V L+ +F
Sbjct: 141 ERDEFERYDKVQNIRPKMVAELRERF 166
Score = 83.4 bits (197), Expect = 2e-16
Identities = 42/78 (53%), Positives = 57/78 (73%)
Frame = +2
Query: 269 VLYLFDVDGTLTKPRQKITEEFRRFILDEVKSKVDVGLVSGSDYMKISEQMGGEDVVSNF 448
V+ LFDVDGTLT PR++ T E FI E++ V +G+V GSD KISEQ+G + V +++
Sbjct: 8 VIALFDVDGTLTAPRKEATPELLDFIR-ELRKVVTIGVVGGSDLSKISEQLG-KTVTNDY 65
Query: 449 NYVFSENGLVHHKNGKKL 502
+Y FSENGLV HK+GK +
Sbjct: 66 DYCFSENGLVAHKDGKSI 83
Score = 39.5 bits (88), Expect = 0.003
Identities = 15/19 (78%), Positives = 16/19 (84%)
Frame = +2
Query: 782 QISIDVFPIGWDKTYCLNH 838
QIS DVFP GWDKTYCL +
Sbjct: 178 QISFDVFPKGWDKTYCLQY 196
>At2g23180.1 68415.m02769 cytochrome P450, putative
Length = 516
Score = 29.9 bits (64), Expect = 2.2
Identities = 20/66 (30%), Positives = 34/66 (51%), Gaps = 7/66 (10%)
Frame = +2
Query: 278 LFDVDGTLTKPRQKITE------EFRRFILDEVKSKVDVGLVSGSDYMKISEQMGG-EDV 436
+F+ D L K +K + +F+RF L + SK++ GLV DY+ +Q+ +DV
Sbjct: 117 IFNADSELWKDLRKSAQSMMTHQDFQRFTLRTIMSKLEKGLVPLLDYVAEKKQVVDLQDV 176
Query: 437 VSNFNY 454
F +
Sbjct: 177 FQRFTF 182
>At4g12430.1 68417.m01967 trehalose-6-phosphate phosphatase,
putative similar to trehalose-6-phosphate phosphatase
(AtTPPB) [Arabidopsis thaliana] GI:2944180; contains
Pfam profile PF02358: Trehalose-phosphatase
Length = 368
Score = 29.1 bits (62), Expect = 3.8
Identities = 19/70 (27%), Positives = 32/70 (45%), Gaps = 4/70 (5%)
Frame = +2
Query: 227 TSFTF*DNYASQKKVLYLFDVDGTLT----KPRQKITEEFRRFILDEVKSKVDVGLVSGS 394
+SF A +KK+ D DGTL+ P + I + R + +V S ++SG
Sbjct: 91 SSFAHIAAQAKKKKIAVFLDYDGTLSPIVDDPDRAIMSDAMRSAVKDVASYFPTAIISGR 150
Query: 395 DYMKISEQMG 424
K+ + +G
Sbjct: 151 SRDKVYQLVG 160
>At2g25870.1 68415.m03105 haloacid dehalogenase-like hydrolase
family protein contains Pfam profiles PF00702: haloacid
dehalogenase-like hydrolase, PF02130: Uncharacterized
protein family UPF0054
Length = 584
Score = 29.1 bits (62), Expect = 3.8
Identities = 21/87 (24%), Positives = 45/87 (51%), Gaps = 8/87 (9%)
Frame = +2
Query: 266 KVLYLF-DVDGTLTKPRQKITEEFRRFILDEVKSKVDVGLVSGSD------YMKISEQMG 424
K Y+F D+DGTL + +I+E + + + + + V + +G +K ++ G
Sbjct: 311 KFSYIFCDMDGTLLNSKSQISEANAKALKEALLRGLKVVIATGKSRPGAIRILKTADLTG 370
Query: 425 GEDVVSNFN-YVFSENGLVHHKNGKKL 502
+ ++S + VF + LV+ + GK++
Sbjct: 371 SDGIISESSPGVFVQGLLVYGRQGKEV 397
>At1g17665.1 68414.m02187 expressed protein
Length = 338
Score = 29.1 bits (62), Expect = 3.8
Identities = 15/39 (38%), Positives = 18/39 (46%)
Frame = -1
Query: 198 SISRTPPENIKCQHFTQTRQTVTVNTKLQYRELINFNNA 82
SIS PE + FT T K QY +L+ NNA
Sbjct: 159 SISENKPEFVCLYPFTSTSSATQRKIKQQYDQLVKCNNA 197
>At5g50090.2 68418.m06203 expressed protein
Length = 153
Score = 28.7 bits (61), Expect = 5.1
Identities = 10/27 (37%), Positives = 19/27 (70%)
Frame = +3
Query: 393 QII*RFPNKWAERTSCPISTTYSVKMD 473
+++ + PN E+ SCP+S +Y +KM+
Sbjct: 11 RVVIQHPNGKEEKLSCPVSASYVMKMN 37
>At5g50090.1 68418.m06202 expressed protein
Length = 159
Score = 28.7 bits (61), Expect = 5.1
Identities = 10/27 (37%), Positives = 19/27 (70%)
Frame = +3
Query: 393 QII*RFPNKWAERTSCPISTTYSVKMD 473
+++ + PN E+ SCP+S +Y +KM+
Sbjct: 11 RVVIQHPNGKEEKLSCPVSASYVMKMN 37
>At4g22590.1 68417.m03259 trehalose-6-phosphate phosphatase,
putative similar to trehalose-6-phosphate phosphatase
(AtTPPA) GI:2944178; contains Pfam profile PF02358:
Trehalose-phosphatase
Length = 377
Score = 28.7 bits (61), Expect = 5.1
Identities = 19/70 (27%), Positives = 30/70 (42%), Gaps = 4/70 (5%)
Frame = +2
Query: 227 TSFTF*DNYASQKKVLYLFDVDGTLT----KPRQKITEEFRRFILDEVKSKVDVGLVSGS 394
TSF A KK+ D DGTL+ P + I + R + +V ++SG
Sbjct: 94 TSFAHIAAQAKNKKIAVFLDYDGTLSPIVDDPDRAIMSDAMRAAVKDVAKYFPTAIISGR 153
Query: 395 DYMKISEQMG 424
K+ + +G
Sbjct: 154 SRDKVYQLVG 163
>At1g74930.1 68414.m08693 AP2 domain-containing transcription
factor, putative similar to AP2 domain containing
protein RAP2.1 GI:2281627 from [Arabidopsis thaliana]
Length = 195
Score = 28.7 bits (61), Expect = 5.1
Identities = 19/60 (31%), Positives = 26/60 (43%), Gaps = 1/60 (1%)
Frame = +2
Query: 287 VDGTLTKPR-QKITEEFRRFILDEVKSKVDVGLVSGSDYMKISEQMGGEDVVSNFNYVFS 463
V+ +LT P Q+ F D VK + + GLV GS+ S S +Y FS
Sbjct: 85 VEKSLTPPEIQEAAARFANTFQDIVKGEEESGLVPGSEIRPESPSTSASVATSTVDYDFS 144
>At4g27595.1 68417.m03964 protein transport protein-related low
similarity to SP|P25386 Intracellular protein transport
protein USO1 {Saccharomyces cerevisiae}
Length = 1212
Score = 28.3 bits (60), Expect = 6.7
Identities = 17/73 (23%), Positives = 37/73 (50%), Gaps = 1/73 (1%)
Frame = +2
Query: 230 SFTF*DNYASQKKVLYLFDVDGTLTKPRQKITEEFRRFILDEVKSKVDVGLVSGSDYMKI 409
+F+ + + K +L+L +V+ K + R+ + +E+K ++ V+ +D MK+
Sbjct: 555 NFSVQEELSKVKNLLHLKEVEACAAKEEDAKMQTNRKELEEEIKDLQEIVEVAKADSMKL 614
Query: 410 SEQM-GGEDVVSN 445
E + ED + N
Sbjct: 615 KESLVEKEDELKN 627
>At5g17680.1 68418.m02072 disease resistance protein (TIR-NBS-LRR
class), putative domain signature TIR-NBS-LRR exists,
suggestive of a disease resistance protein.
Length = 1294
Score = 27.9 bits (59), Expect = 8.9
Identities = 11/34 (32%), Positives = 18/34 (52%)
Frame = -3
Query: 376 YIHF*FYLVKNEPPEFFCNFLARFCQRSVNIEQI 275
Y+ + Y +K P FF FL C + N+E++
Sbjct: 584 YLRWDGYPLKTMPSRFFPEFLVELCMSNSNLEKL 617
>At4g18970.1 68417.m02794 GDSL-motif lipase/hydrolase family protein
similar to family II lipases EXL3 GI:15054386, EXL1
GI:15054382, EXL2 GI:15054384 from [Arabidopsis
thaliana]; contains Pfam profile PF00657: GDSL-like
Lipase/Acylhydrolase
Length = 361
Score = 27.9 bits (59), Expect = 8.9
Identities = 16/53 (30%), Positives = 28/53 (52%), Gaps = 2/53 (3%)
Frame = +2
Query: 359 KSKVDV--GLVSGSDYMKISEQMGGEDVVSNFNYVFSENGLVHHKNGKKLSSR 511
K+ VDV L+ DY+ + GED++ NY + G + + G++L +R
Sbjct: 72 KTTVDVITELLGFDDYITPYSEARGEDILRGVNYASAAAG-IREETGRQLGAR 123
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 18,006,751
Number of Sequences: 28952
Number of extensions: 375007
Number of successful extensions: 915
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 892
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 913
length of database: 12,070,560
effective HSP length: 80
effective length of database: 9,754,400
effective search space used: 1941125600
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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